1. Developmental Biology and Stem Cells
  2. Plant Biology
Download icon

Cell type boundaries organize plant development

  1. Monica Pia Caggiano
  2. Xiulian Yu
  3. Neha Bhatia
  4. André Larsson
  5. Hasthi Ram
  6. Carolyn K Ohno
  7. Pia Sappl
  8. Elliot M Meyerowitz
  9. Henrik Jönsson
  10. Marcus G Heisler Is a corresponding author
  1. European Molecular Biology Laboratory, Germany
  2. Lund University, Sweden
  3. California Institute of Technology, United States
Research Article
Cited
0
Views
913
Comments
0
Cite as: eLife 2017;6:e27421 doi: 10.7554/eLife.27421

Abstract

In plants the dorsoventral boundary of leaves defines an axis of symmetry through the centre of the organ separating the top (dorsal) and bottom (ventral) tissues. Although the positioning of this boundary is critical for leaf morphogenesis, how the boundary is established and how it influences development remains unclear. Using live-imaging and perturbation experiments we show that leaf orientation, morphology and position are pre-patterned by HD-ZIPIII and KAN gene expression in the shoot, leading to a model in which dorsoventral genes coordinate to regulate plant development by localizing auxin response between their expression domains. However we also find that auxin levels feedback on dorsoventral patterning by spatially organizing HD-ZIPIII and KAN expression in the shoot periphery. By demonstrating that the regulation of these genes by auxin also governs their response to wounds, our results also provide a parsimonious explanation for the influence of wounds on leaf dorsoventrality.

Article and author information

Author details

  1. Monica Pia Caggiano

    European Molecular Biology Laboratory, Heidelberg, Germany
    Competing interests
    The authors declare that no competing interests exist.
  2. Xiulian Yu

    European Molecular Biology Laboratory, Heidelberg, Germany
    Competing interests
    The authors declare that no competing interests exist.
  3. Neha Bhatia

    European Molecular Biology Laboratory, Heidelberg, Germany
    Competing interests
    The authors declare that no competing interests exist.
  4. André Larsson

    Computational Biology and Biological Physics, Department of Astronomy and Theoretical Physics, Lund University, Lund, Sweden
    Competing interests
    The authors declare that no competing interests exist.
  5. Hasthi Ram

    European Molecular Biology Laboratory, Heidelberg, Germany
    Competing interests
    The authors declare that no competing interests exist.
  6. Carolyn K Ohno

    European Molecular Biology Laboratory, Heidelberg, Germany
    Competing interests
    The authors declare that no competing interests exist.
  7. Pia Sappl

    European Molecular Biology Laboratory, Heidelberg, Germany
    Competing interests
    The authors declare that no competing interests exist.
  8. Elliot M Meyerowitz

    Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon 0000-0003-4798-5153
  9. Henrik Jönsson

    Computational Biology and Biological Physics, Department of Astronomy and Theoretical Physics, Lund University, Lund, Sweden
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon 0000-0003-2340-588X
  10. Marcus G Heisler

    European Molecular Biology Laboratory, Heidelberg, Germany
    For correspondence
    marcus.heisler@sydney.edu.au
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon 0000-0001-5644-8398

Funding

H2020 European Research Council (261081)

  • Marcus G Heisler

Marie Curie Actions (255089)

  • Pia Sappl

Gordon and Betty Moore Foundation (GBMF3406)

  • Elliot M Meyerowitz

Gatsby Charitable Foundation (GAT3395/PR4)

  • Henrik Jönsson

Swedish Research Council (VR2013-4632)

  • Henrik Jönsson

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Dominique C Bergmann, Reviewing Editor, Stanford University/HHMI, United States

Publication history

  1. Received: April 3, 2017
  2. Accepted: September 11, 2017
  3. Accepted Manuscript published: September 12, 2017 (version 1)

Copyright

© 2017, Caggiano et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

Metrics

  • 913
    Page views
  • 239
    Downloads
  • 0
    Citations

Article citation count generated by polling the highest count across the following sources: PubMed Central, Scopus, Crossref.

Comments

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Downloads (link to download the article as PDF)

Download citations (links to download the citations from this article in formats compatible with various reference manager tools)

Open citations (links to open the citations from this article in various online reference manager services)

Further reading

    1. Cell Biology
    2. Genes and Chromosomes
    Wahid A Mulla et al.
    Research Article Updated