Microtubule plus-end dynamics link wound repair to the innate immune response
- Aix Marseille Université, INSERM, CNRS, France
- Aix Marseille Université, CNRS UMR7332, France
Abstract
The skin protects animals from infection and physical damage. In C. elegans, wounding the epidermis triggers an immune reaction and a repair response, but it is not clear how these are coordinated. Previous work implicated the microtubule cytoskeleton in the maintenance of epidermal integrity (Chuang et al, 2016). Here, by establishing a simple wounding system, we show that wounding provokes a reorganisation of plasma membrane subdomains. This is followed by recruitment of the microtubule plus end-binding protein EB1/EBP-2 around the wound and actin ring formation, dependant on ARP2/3 branched actin polymerisation. We show that microtubule dynamics are required for the recruitment and closure of the actin ring, and for the trafficking of the key signalling protein SLC6/SNF-12 towards the injury site. Without SNF-12 recruitment, there is an abrogation of the immune response. Our results suggest that microtubule dynamics coordinate the cytoskeletal changes required for wound repair and the concomitant activation of innate immunity.
Data availability
All data generated or analysed during this study are included in the manuscript and supporting files. Source data files have been provided for all quantitative Figures.
Article and author information
Author details
Sébastien Mailfert
Jean-François Rupprecht
Funding
Agence Nationale de la Recherche (ANR-16-CE15-0001-01)
- Clara Taffoni
- Sébastien Mailfert
- Matthieu Fallet
- Jonathan Ewbank
- Nathalie Pujol
Institut National de la Santé et de la Recherche Médicale
- Jonathan Ewbank
Centre National de la Recherche Scientifique
- Shizue Omi
- Sébastien Mailfert
- Matthieu Fallet
- Jean-François Rupprecht
- Nathalie Pujol
Aix-Marseille Université
- Clara Taffoni
- Caroline Huber
Agence Nationale de la Recherche (ANR-12-BSV3-0001-01)
- Clara Taffoni
- Sébastien Mailfert
- Matthieu Fallet
- Jonathan Ewbank
- Nathalie Pujol
Agence Nationale de la Recherche (ANR-11-LABX-0054)
- Clara Taffoni
- Sébastien Mailfert
- Matthieu Fallet
- Jonathan Ewbank
- Nathalie Pujol
Agence Nationale de la Recherche (ANR-11-IDEX-0001-02)
- Clara Taffoni
- Sébastien Mailfert
- Matthieu Fallet
- Jonathan Ewbank
- Nathalie Pujol
Agence Nationale de la Recherche (ANR-10-INBS-04-01)
- Clara Taffoni
- Sébastien Mailfert
- Matthieu Fallet
- Jonathan Ewbank
- Nathalie Pujol
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Terry Lechler, Duke University, United States
Version history
- Received: January 11, 2019
- Accepted: January 27, 2020
- Accepted Manuscript published: January 29, 2020 (version 1)
- Version of Record published: February 26, 2020 (version 2)
Copyright
© 2020, Taffoni et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Microtubule plus-end dynamics link wound repair to the innate immune response
eLife 9:e45047.
https://doi.org/10.7554/eLife.45047
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