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Identification of TMEM206 proteins as pore of PAORAC/ASOR acid-sensitive chloride channels

  1. Florian Ullrich
  2. Sandy Blin
  3. Katina Lazarow
  4. Tony Daubitz
  5. Jens Peter von Kries
  6. Thomas J Jentsch  Is a corresponding author
  1. Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Germany
  2. Max-Delbrück-Centrum für Molekulare Medizin (MDC), Germany
  3. Charité Universitätsmedizin, Germany
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Cite this article as: eLife 2019;8:e49187 doi: 10.7554/eLife.49187


Acid-sensing ion channels have important functions in physiology and pathology, but the molecular composition of acid-activated chloride channels had remained unclear. We now used a genome-wide siRNA screen to molecularly identify the widely expressed acid-sensitive outwardly-rectifying anion channel PAORAC/ASOR. ASOR is formed by TMEM206 proteins which display two transmembrane domains (TMs) and are expressed at the plasma membrane. Ion permeation-changing mutations along the length of TM2 and at the end of TM1 suggest that these segments line ASOR’s pore. While not belonging to a gene family, TMEM206 has orthologs in probably all vertebrates. Currents from evolutionarily distant orthologs share activation by protons, a feature essential for ASOR’s role in acid-induced cell death. TMEM206 defines a novel class of ion channels. Its identification will help to understand its physiological roles and the diverse ways by which anion-selective pores can be formed.



Chloride is by far the most abundant anion in animals. Its concentration can vary substantially between the extracellular space, the cytoplasm and various intracellular organelles, resulting in concentration gradients across membranes separating these compartments. Negatively charged chloride can cross biological membranes only with the help of membrane-spanning proteins such as Cl- channels, which allow passive diffusion of Cl- along its electrochemical gradient, or transporter proteins that couple the movement of Cl- to that of other ions and can thereby establish electrochemical gradients.

Chloride channels fulfill a broad range of biological functions, including the homeostasis of cell volume, vesicular acidification, transepithelial transport and cellular signaling (Jentsch and Pusch, 2018; Jentsch et al., 2002). Elucidation of these roles has been greatly facilitated by the molecular identification of the underlying channel proteins, a discovery process that began in the late 1980’s and is still ongoing. Chloride channels are molecularly and structurally very diverse and lack a defining ‘signature’ pattern. More than six unrelated Cl- channel families are known, prominently including voltage-regulated CLC channels (Jentsch and Pusch, 2018; Jentsch et al., 1990), bestrophin (Hartzell et al., 2008; Sun et al., 2002) and TMEM16 (Caputo et al., 2008; Schroeder et al., 2008; Yang et al., 2008) Ca2+-activated Cl- channels, as well as LRRC8/VRAC volume-regulated anion channels (Qiu et al., 2014; Voss et al., 2014). However, several Cl- currents biophysically characterized in mammalian cells still lack molecular correlates, severely hindering the elucidation of their cellular and organismal functions.

We set out to identify the protein(s) mediating a widely expressed, strongly outwardly-rectifying plasma membrane Cl- current ICl,H that is detectable only upon marked extracellular acidification and which may play a role in acid-induced cell death (Auzanneau et al., 2003; Capurro et al., 2015; Lambert and Oberwinkler, 2005; Sato-Numata et al., 2013; Wang et al., 2007). The underlying channel is most frequently called ASOR (for Acid-Sensitive Outwardly Rectifying anion channel; Wang et al., 2007), although the earlier name PAORAC (Proton-Activated Outwardly Rectifying Anion Channel; Lambert and Oberwinkler, 2005; Ma et al., 2008) provides a better description. At room temperature, ASOR currents are only observable when external pH (pHo) drops below 5.5, but the threshold of activation shifts to ~6.0 at 37°C (Sato-Numata et al., 2013). Analysis of the steep pH-dependence suggests that 3–4 protons are required to open the channel (Capurro et al., 2015; Lambert and Oberwinkler, 2005). ASOR currents are strongly outwardly rectifying with almost no currents being observable at negative-inside voltages. This rectification results both from voltage-dependent gating that operates on a 100 ms time scale and from an outwardly-rectifying single channel conductance (Lambert and Oberwinkler, 2005) (although others described that single ASOR channels are not rectifying, for example Wang et al., 2007). ASOR currents (ICl,H) display an SCN->I->NO3->Br->Cl- permeability sequence. The channel can be blocked by various Cl- transport inhibitors such as DIDS (4,4'-diisothiocyano-2,2'-stilbenedisulfonic acid) and niflumic acid (Capurro et al., 2015; Lambert and Oberwinkler, 2005) and by other compounds such as phloretin (Wang et al., 2007) and pregnenolone sulfate (PS) (Drews et al., 2014). However, none of these inhibitors is specific for ASOR.

The observation of ICl,H currents in every investigated mammalian cell type (Auzanneau et al., 2003; Capurro et al., 2015; Fu et al., 2013; Lambert and Oberwinkler, 2005; Ma et al., 2008; Nobles et al., 2004; Sato-Numata et al., 2013; Valinsky et al., 2017; Wang et al., 2007; Yamamoto and Ehara, 2006) suggests that ASOR may be expressed in all tissues. Such a wide expression pattern indicates that this channel has important physiological functions. However, only few mammalian cells are physiologically exposed to an extracellular pH that is acidic enough to open ASOR. It was therefore proposed (Wang et al., 2007) that the channel rather plays a role in pathologies such as cancer or ischemic stroke in which pHo can drop to pH 6.5 or below (Gillies et al., 2018; Kato et al., 2013; Nedergaard et al., 1991; Thews and Riemann, 2019; Xiong et al., 2004). Indeed, ASOR inhibitors blunted cell swelling and cell death provoked by prolonged exposure to acidic pHo (Sato-Numata et al., 2014; Wang et al., 2007), suggesting that ASOR-mediated chloride influx may worsen the outcome of ischemic stroke. It seems, however, counterintuitive that a channel that enhances cell death under pathological conditions confers an evolutionary advantage. Alternatively, ASOR might not only be present at the plasma membrane, but also in intracellular compartments such as lysosomes where it would be exposed to an appropriate acidic pH (Lambert and Oberwinkler, 2005).

The molecular identity of ASOR had remained unknown, with several candidates such as LRRC8/VRAC anion channels or ClC-3 Cl-/H+ exchangers having been excluded previously (Lambert and Oberwinkler, 2005; Sato-Numata et al., 2016; Sato-Numata et al., 2013). Using a genome-wide siRNA screen we now identified TMEM206 as essential ASOR component. TMEM206, which has two transmembrane domains, was necessary and sufficient for the formation of functional ASOR channels. All tested TMEM206 orthologs from other vertebrates form acid-activated channels which, however, differ moderately in their biophysical properties. Ion-selectivity changing mutations suggest that TMEM206’s transmembrane domains, in particular TM2, form ASOR’s pore. Finally, partial protection of TMEM206−/− cells from acid-induced cell death indicates that ASOR/TMEM206 channels play a detrimental role in pathological conditions that are associated with tissue acidosis.


Identification of TMEM206 as crucial ASOR component

To identify the protein(s) constituting ASOR, we performed a genome-wide siRNA screen using an optical assay for acute ASOR-mediated iodide influx into HeLa cells (Figure 1A). Intracellular iodide was detected by fluorescence quenching of inducibly expressed, halide-sensitive and relatively pH-insensitive E2GFP (Arosio et al., 2007). We activated ASOR by exposing cells to acidic extracellular pH (5.0) and simultaneously depolarized their plasma membrane to maximize ASOR currents. To this end, we used an approach which we had used previously (Scheel et al., 2005) to activate ClC-5, a Cl-/H+-exchanger that displays a similarly strong outward rectification. In HeLa cells we stably co-expressed E2GFP with FaNaC (Lingueglia et al., 1995), a ligand-gated Na+ channel from the snail Cornu aspersum that is closed until it is activated by the cognate neurotransmitter peptide Phe-Met-Arg-Phe-NH2 (FMRFamide). FMRFamide application leads to Na+ influx that acutely depolarizes the membrane to inside-positive voltages. Concomitant application of 100 mM iodide, pH 5.0 and 20 µM FMRFamide to our engineered HeLa cells induced fast quenching of E2GFP fluorescence (Figure 1B). Control experiments omitting either iodide, acidic pHo, or FMRFamide indicated that a major component of quenching is owed to iodide influx through an acidic pH- and depolarization-dependent process such as ASOR (Figure 1B, Figure 1—figure supplement 1A–D). This notion was further supported by applying the inhibitors pregnenolone sulfate and DIDS (Drews et al., 2014) (Figure 1—figure supplement 1E,F).

Figure 1 with 1 supplement see all
Identification of TMEM206 as ASOR component.

(A) Screening assay. Engineered HeLa cells inducibly expressing iodide-sensitive E2GFP and FRMFamide-gated Na+ channel FaNaC were acutely exposed to an acidic solution (pH 5) containing 20 μM FMRFamide and 100 mM I-. Iodide influx through ASOR is stimulated both by acidic pH and the depolarization caused by FaNaC-mediated Na+-influx and induces quenching of E2GFP fluorescence. Fluorescence quenching is reduced by ASOR knock-down. (B) Assay verification under the conditions used for screening. Addition of I- and FRMFamide at pH 5 (arrow) induces rapid quenching of fluorescence. Less rapid quenching upon omission of either I-, FMRFamide or acidic pH suggests that it is caused by I- influx through ASOR, as further supported by inhibition by PS (pregnenolone sulfate) and DIDS (4,4'-diisothiocyano-2,2'-stilbenedisulfonic acid) (Figure 1—figure supplement 1). (C) Fluorescence curves from a 384-well plate treated with siRNA against 280 genes, including TMEM206. Fluorescence quenching is specifically slowed by siRNA against TMEM206. (D) Distribution of median Z-scores (mean of 3 replicates) from filtered hits (see Materials and methods). TMEM206 was the top hit. (E) Tissue expression of TMEM206 extracted from the GTEx database (https://gtexportal.org/home/; TPM, transcripts per million). (F) Dendrogram depicting similarity between TMEM206 orthologs from human (Homo sapiens), African naked mole-rat (Heterocephalus glaber), chicken (Gallus gallus), green anole lizard (Anolis carolinensis), zebrafish (Danio rerio) and from the hemichordate acorn worm (Saccoglossus kowalewski). Amino-acid sequence identity to human TMEM206 given in brackets. Dendrogram based on a Clustal Omega protein alignment fed into the Simple Phylogeny tool at EMBL-EBI (https://www.ebi.ac.uk/services).


Having established a sensitive assay for ASOR function, we performed a genome-wide siRNA screen using a commercial library containing pools of four siRNAs per gene (Figure 1C). The screen was carried out in triplicate and results were ranked according to the maximal slope of quenching. The top hit was TMEM206, a so far uncharacterized membrane protein (Figure 1D). The wide tissue expression pattern of TMEM206 indicated by public databases (Figure 1E) agreed with the finding that ICl,H has been found in every cell studied so far (Auzanneau et al., 2003; Capurro et al., 2015; Fu et al., 2013; Lambert and Oberwinkler, 2005; Ma et al., 2008; Nobles et al., 2004; Sato-Numata et al., 2013; Valinsky et al., 2017; Wang et al., 2007). TMEM206 has orthologs in vertebrates and in the hemichordate Saccoglossus kowalevskii (acorn worm) (Figure 1F), but there are no homologs in simpler animals or other kingdoms of life. In contrast to many other ion channels, TMEM206 does not form a gene family because it lacks paralogs within the same species (i.e. it is a ‘single gene’).

After transfection of GFP-tagged human TMEM206 (hTMEM206) into HeLa cells a significant fraction of the protein was found at the plasma membrane (Figure 2A). Additionally, TMEM206 appeared to be expressed in intracellular membranes, which, however, might result from heterologous overexpression. Hydropathy analysis suggested the presence of two transmembrane spans (Figure 2B). To determine the transmembrane topology of TMEM206, we tested the accessibility of added epitopes by immunofluorescence. Detection of both N- or C-terminally added GFP required permeabilization of the plasma membrane (Figure 2C,D), suggesting that both the amino- and the carboxy-terminus of TMEM206 face the cytosol.

Subcellular localization and transmembrane topology of TMEM206.

(A) Subcellular localization of TMEM206 (fused to GFP at the C-terminus) in transfected HeLa cells. A similar localization was observed when the tag was attached to the N-terminus. Scale bar: 10 μm (B) Hydropathy analysis of TMEM206 using the TMpred server (https://embnet.vital-it.ch/software/TMPRED_form.html) suggests the presence of two transmembrane domains. (C, D) Detection of GFP by its fluorescence (green) or immunocytochemistry (red) in cells transfected with GFP-TMEM206 (C) or TMEM206-GFP (D), without (top panels) or with (lower panels) plasma membrane permeabilization. Scale bars: 10 μm. (E) A HA-epitope inserted after residue 271 between TM1 and TM2 (G) in the TMEM206-exHA mutant was detected in non-permeabilized cells. (F) Western blot of membranes from HEK cells transfected with TMEM206-GFP or TMEM206(Δglyc)-GFP in which all four predicted N-linked glycosylation sites between TM1 and TM2 were disrupted by mutagenesis. Note the lower molecular weight of TMEM206(Δglyc)-GFP. Deglycosylation of membrane proteins by PNGaseF reduced the molecular weight of the WT, but not the TMEM206(Δglyc)-GFP protein. (G) Schematic topology of TMEM206. Predicted glycosylation sites (N), and phosphorylated sites (P) identified in mass spectrometry (https://www.phosphosite.org/) are indicated, as well as the location of added epitopes.


A cytosolic localization of the N-terminus is further supported by public databases showing that the amino-terminus of human and rodent TMEM206 can be phosphorylated (Figure 2G). An extracellular localization of the TM1-TM2 stretch was suggested by the immunofluorescent detection in non-permeabilized cells of an HA-epitope inserted at position 271 (Figure 2E,G). The observation that this stretch is glycosylated further supports its extracellular localization (Figure 2F). Only this segment displays consensus sites (N148, N155, N162, N190) for N-linked glycosylation (Figure 2G, Figure 6—figure supplement 2). Deglycosylation of WT and mutant TMEM206(Δglyc), in which all four consensus sites were eliminated, demonstrated that at least one of these sites are used (Figure 2F) and hence face the lumen of the ER during biogenesis. We conclude that both N- and C-termini of TMEM206 reside in the cytosol, whereas the TM1-TM2 loop faces the extracellular space (Figure 2G).

Human TMEM206 mediates typical ASOR currents

Overexpression of human TMEM206 in HEK cells increased acid-activated Cl- currents about 10-fold (Figure 3A,B). Addition of GFP to either the amino- or carboxy-terminus of TMEM206 only moderately decreased current amplitudes compared to the untagged construct (Figure 3B). Currents from overexpressed hTMEM206, irrespective of whether fused to GFP, resembled native ICl,H. To confirm that TMEM206 is essential for the generation of ICl,H, we disrupted the TMEM206 gene in both HEK and HeLa cells using CRISPR-Cas9 genomic editing. To exclude off-target effects, we used three different gRNAs to generate independent KO clones. None of the clonal HEK or HeLa KO cell lines displayed measurable ICl,H currents (Figure 3C–E, Figure 3—figure supplement 1). Hence, TMEM206 is an indispensable component of ASOR.

Figure 3 with 1 supplement see all
TMEM206 mediates ICl,H ASOR currents.

(A) Voltage-clamp traces of non-transfected (n.t.) and TMEM206-GFP transfected HEK cells at pHo = 4.8, using the protocol shown at the top. (B) Current densities (I/C, at indicated pHo) at +80 and −80 mV for non-transfected (n.t.) HEK cells and cells transfected with human TMEM206 (T6) fused N- or C-terminally to GFP, or co-expressing untagged TMEM206 with GFP from a separate pEGFP-N1 vector. Transfection increased current levels by 5- to 15-fold (bars, mean). (C) CRISPR-Cas9 mediated genomic disruption of TMEM206 in HEK cells (by guide RNA g1) abolished native acid-activated ASOR currents (ICl,H) as determined at pHo 4.8. Clamp protocol as in (A). (D) Outward and (smaller) inward ICl,H currents rapidly activate in WT, but not TMEM206−/− HEK cells when pHo is lowered from 7.4 to 4.8. (E) Native ICl,H of HEK cells is abolished in three independent TMEM206−/− cell lines generated with different guide RNAs (g1 – g3). (bars, mean; ***, p<0.001, one-way ANOVA with Bonferroni correction) (F) Voltage-clamp traces of TMEM206−/− HEK cells transfected with human TMEM206 reveal large ICl,H. Clamp protocol as in (A). (G) Rapid activation and deactivation of ICl,H in TMEM206-transfected TMEM206−/− HEK cells when pHo is changed between 7.4 and 4.8. Currents monitored using a ramp protocol. (H) ICl,H densities at indicated voltages and pHo of non-transfected (n.t.) or TMEM206-transfected TMEM206−/− HEK cells (bars, mean; ***, p<0.001, one-way ANOVA with Bonferroni correction). (I) pHo-dependence of ICl,H (at +80 mV) from native HEK cells and TMEM206-transfected TMEM206−/− HEK cells. pH50, pHo at which current is half maximal.

Table 1
TMEM206 Knockout Cell Lines.
Cell lineClonesgRNA sequenceGenetic modificationProtein modificationFigures
1D4CAGCTGTAAGCACCATTACGa1: duplication of a395
a2: Δa395
1: Y132stop between TMD1 and TMD2
2: Y132S-fs between TMD1 and TMD2
2E5GGACCGAGAAGACGTTCTTCHomozygous Δ2nt (g189-a190)N64R-fs before TMD13 C-H; 4; 5; 6; 6-S1; 7; 7-S1; 8;
3D9AAGGAGACGGTCAGAGTCCAHomozygous duplication of t110Q38P-fs before TMD13E; 8A-B
1G1CAGCTGTAAGCACCATTACGduplication of a395Y132stop between TMD1 and TMD23-S1
2C6GGACCGAGAAGACGTTCTTCa1: Δ11nt (g178-a188) a2: duplication of g1891: A60E-fs
2: N64E-fs before TMD1
3F8AAGGAGACGGTCAGAGTCCAduplication of t110Q38P-fs before TMD12A,C,D; 8C,D; 3-S1
  1. a = allele; fs = frameshift; nt = nucleotide; TMD = transmembrane domain; Δ deletion.

Transfection of hTMEM206 rescued ICl,H currents of TMEM206−/− (KO) HEK and HeLa cells (Figure 3F–H, Figure 3—figure supplement 1E). Current characteristics did not differ between transfected WT or KO cells (Figure 3A,F). TMEM206−/− HEK cells were used to further characterize hTMEM206 in transient overexpression.

Currents of overexpressed hTMEM206 (measured at acidic pHo) showed the typical outward-rectification and activation kinetics of ASOR (Capurro et al., 2015; Lambert and Oberwinkler, 2005; Wang et al., 2007) after depolarizing voltage steps (Figure 3C,F). However, I/V curves for these measurements must be interpreted with some care since the very large currents (>15 nA) and the absence of series resistance compensation may have resulted in confounding voltage clamp errors. The pHo-dependence of native ICl,H was indistinguishable from that of TMEM206-transfected KO HEK cells (Figure 3I,H). The extent of acidification needed for half-activation of ICl,H (pHo ~5.3) agrees with previous studies (Capurro et al., 2015; Lambert and Oberwinkler, 2005; Sato-Numata et al., 2013; Wang et al., 2007).

Measurements of reversal potentials of hTMEM206-transfected cells (Figure 4A,B) revealed a SCN->I->NO3->Br->Cl- permeability sequence that is typical for ASOR (Capurro et al., 2015; Lambert and Oberwinkler, 2005). Replacement of extracellular Cl- by either gluconate- (Figure 4C) or SO42- (Figure 4D) not only abolished outward, but also inward currents which are carried by anion efflux. Since we had replaced extracellular, but not intracellular Cl-, these observations suggest that gluconate- and SO42- (or HSO4-, a minor species with which SO42- is at equilibrium and whose abundance will be increased by the acidic pH) block the outward movement of Cl- through ASOR/TMEM206 channels. Alternatively, ASOR may require activation by extracellular chloride. Native ICl,H is inhibited by several compounds (Capurro et al., 2015; Drews et al., 2014; Lambert and Oberwinkler, 2005), but none of these is specific for ASOR. Acid-activated currents from overexpressed TMEM206 were efficiently blocked by DIDS, niflumic acid, and pregnenolone sulfate (PS, Figure 4E–H). As described for native ICl,H (Drews et al., 2014), the block by PS was fast and reversible (Figure 4F) and affected both outward and inward currents (Figure 4G). We conclude that overexpressed TMEM206 shares all essential properties with native ASOR currents (ICl,H).

Ion selectivity and inhibitor sensitivity of TMEM206/ASOR channels.

(A) Example traces of ICl,H (elicited by voltage ramps) from TMEM206-transfected TMEM206−/− HEK cells upon equimolar replacement of extracellular NaCl (150 mM) by the sodium salts of the indicated anions. The intracellular solution contained 150 mM CsCl. The voltage at which I = 0 defines the reversal potential. (B) Reversal potentials Erev. (C) Upon replacement of extracellular Cl- by gluconate, or (D) by HSO4-/SO42-, no currents discernible from background were detected (at pHo 4.8). (E) Mean inhibition (%) of ICl,H from TMEM206-transfected TMEM206−/− HEK cells (measured at +80 mV, pHo 4.8) by various inhibitors of ASOR. PS, pregnenolone sulfate; DIDS, 4,4'-diisothiocyano-2,2'-stilbenedisulfonic acid; NFA, niflumic acid. (F) Fast and reversible block of ICl,H by PS. (G) Example I/V curves of ICl,H from TMEM206-transfected TMEM206−/− HEK cells exposed to different PS concentrations. (H) Same for block by DIDS.


TMEM206 proteins from other species mediate moderately different ICl,H currents

We asked whether TMEM206 proteins from other clades also function as ASORs and transfected orthologs from the green anole lizard (Anolis carolinensis), chicken (Gallus gallus), zebrafish (Danio rerio) and from the hemichordate acorn worm (Saccoglossus kowalewski) into TMEM206−/− HEK cells. We also tested TMEM206 from naked mole-rats (Heterocephalus glaber), mammals displaying various unusual properties including insensitivity to acidic pain (Browe et al., 2018; Edrey et al., 2011; Park et al., 2008) and decreased acid-induced neuronal cell death (Husson and Smith, 2018).

Except for the evolutionarily distant hemichordate protein, which was retained in the endoplasmic reticulum (ER), all orthologs were expressed at the plasma membrane (Figure 5—figure supplement 1). All plasma-membrane expressed orthologs mediated robust acid-activated outwardly rectifying ICl,H currents (Figure 5A,C). These currents, however, differed in detail. Strikingly, TMEM206 from the reptile Anolis carolinensis mediated outwardly-rectifying currents already at pHo 7.4. These currents were further steeply activated when pHo dropped below 6 (Figure 5A–D). Whereas currents from all orthologs activated at a threshold of ~pHo 6, their currents decreased differently at pHo <5 (Figure 5D). The current decrease at more acidic pH questions the reliability of determining Hill coefficients for the activation of ASOR by H+ ions (Capurro et al., 2015; Lambert and Oberwinkler, 2005; Yang et al., 2019). Differences between orthologs were also observed in depolarization-activated gating and current rectification (Figure 5A,D), and most importantly in anion permeability (Figure 5E–I). Whereas all orthologs displayed the same Cl- permeability (Figure 5E,I), the mammalian ASORs (human and naked mole-rat) showed a higher iodide/chloride permeability ratio than their chicken, anole and zebrafish orthologs (Figure 5F,I). ASORs from green anole and zebrafish displayed a significant SO42- (or HSO4-) permeability which was undetectable with the other tested orthologs (Figure 5G–I). These results suggest that all vertebrate TMEM206 proteins mediate ASOR currents and participate in forming its ion-selective pore.

Figure 5 with 1 supplement see all
Properties of TMEM206 orthologs.

(A) Typical voltage clamp-traces of indicated TMEM206 orthologs transiently expressed in TMEM206−/− HEK cells at pHo 7.4 (top traces) or pHo 5.0 (bottom traces). Clamp protocol as in Figure 3A. (B) Mean current amplitudes of tested orthologs at pHo7.4 and (C) pHo 5.0. The ortholog from acorn worm failed to give currents because of its retention in the endoplasmic reticulum (Figure 5—figure supplement 1F). (D) pHo-dependence of currents from various orthologs at +80 mV (top) and –80 mV (bottom) as determined from voltage ramps. (E–G) Reversal potentials Erev of indicated orthologs with external NaCl (E), Nal (F) and Na2SO4 (G). Significant currents with Na2SO4 could be measured only for green anole and zebrafish. ΔErev, difference to Erev for NaCl. All currents measured at pHo 5.25. (H) Current densities (at +80 mV, pHo 5.25) with external Na2SO4 (I) Example traces showing determination of Erev (indicated by arrows) for human, chicken and zebrafish orthologs. bg, background current at pHo 7.4 (n = 8–13 cells; *, p<0.033; ***, p<0.001; Kruskal-Wallis test, Dunn’s multiple comparison correction; error bars, SD (B,C) or SEM (D–H)).


Cysteine-scanning mutagenesis reveals pore-lining residues of TMEM206

To identify pore-lining residues of ASOR we used a systematic substituted-cysteine accessibility approach (Holmgren et al., 1996). After having ascertained that the cysteine reagent MTSES lacks significant effects on human WT channels (Figure 6A,B), we generated point mutants in which all residues of both hTMEM206 transmembrane spans were singly replaced by cysteines (Figure 6B). Except for two mutants in TM2 (L309C and K319C), all constructs elicited acid-activated ICl,H currents when transfected into TMEM206−/− HEK cells (Figure 6B,C, Figure 6—figure supplement 1). However, current amplitudes and properties were often changed (Figure 6—figure supplement 1). Only one mutant in TM1 (L84C) showed a marked response to cysteine modification (Figure 6B,C). Application of MTSES to this mutant, which changes a residue close to the predicted external end of TM1, increased currents selectively at negative voltages (Figure 6B,C). TM2, which is less hydrophobic than TM1 (Figure 2B) and may form an amphipathic α-helix (Figure 6—figure supplement 2), contained a larger number of responsive cysteine-substituted residues (Figure 6B,C). These residues were not only found close to the external end of TM2 (W304), but also close to the center (G312) and more towards the cytoplasmic end of TM2 (L315, A316) (Figure 6B,C). These residues failed to clearly cluster on one side of the postulated α-helix (Figure 6—figure supplement 2A). Depending on the mutant, MTSES exposure increased or decreased ICl,H amplitudes (Figure 6A–C) or changed current rectification (Figure 6B–C). Consistent with a covalent modification of cysteine residues, the effects of MTSES appeared irreversible (Figure 6A). Strikingly, several cysteine mutants showed robust outwardly-rectifying currents already at pHo 7.4 (Figure 6D,E). These could be further stimulated by exposure to acidic pHo (Figure 6F).

Figure 6 with 2 supplements see all
Substituted cysteine accessibility scan of TMEM206 transmembrane domains.

(A) Effect of MTSES on currents from WT and mutant TMEM206-transfected TMEM206−/− cells. MTSES had no effect on WT TMEM206, but acutely and irreversibly decreased or increased ICl,H of I307C and L315C mutants, respectively. Note that acidic pHo does not interfere with MTSES reactivity. The protocol was designed to be able to detect potential effects of MTSES at pH 7.4. (B) Ratio of current before (Ictrl) and after (IMTSES) exposure to MTSES, at +80 and −80 mV. Mutants are grouped by location within the two TMDs of TMEM206. For current amplitudes of non-modified cysteine mutants, see Figure 6—figure supplement 1. (C) Current traces of selected mutants elicited by voltage ramps (top) before and after MTSES-application. (D–F) Cysteine mutants showing currents at pHo 7.4. (D) Voltage-clamp traces of WT TMEM206 and indicated mutants at pH 7.4. Clamp protocol as in Figure 3A. (E) Mean current densities at pH 7.4. (F) All mutants still responded to low pHo.


Of note, some cysteine mutants displayed changed ion selectivity (Figure 7A,B,F–H). Whereas changes in I- permeability were relatively small (most evident for L84C and R87C, Figure 7B,F), the same two mutants in TM1 displayed a small sulfate permeability that was undetectable in WT hTMEM206, allowing us to determine reversal potentials (Figure 7A,B,G–H). Residues giving an interesting effect upon cysteine modification were further mutated to other, mostly charged, residues. This yielded detectable ICl,H in many cases (Figure 7—figure supplement 1), with a number of mutants displaying slightly changed iodide permeability (Figure 7C,D,F) or marked increases in SO42-/HSO4- conductance (Figure 7C,G–H). Strikingly, replacement of L315 by negatively charged aspartate introduced a large ~22 mV shift of the reversal potential in NaCl medium (Figure 7D,E), indicative of a loss of anion selectivity. Together with the strong MTSES effects on the L315C mutant (Figure 6A–C) these data suggest that this TM2 residue importantly determines pore properties of ASOR. Of note, with the exception of R87, all functionally important residues identified here in TM1 and TM2 are highly conserved (Figure 6—figure supplement 2B). In conclusion, residues along the length of TM2 and towards the external end of TM1 may be close to, or line, the pore of ASOR channels.

Figure 7 with 1 supplement see all
Ion selectivity changes of mutants in transmembrane domains.

(A–D) Current traces of WT (A), L84C (B), I307W (C) and L315D (D) TMEM206 expressed in TMEM206−/− HEK cells measured with indicated extracellular solutions at pH 5.25. Reversal potentials Erev indicated by arrows. With WT and L315D TMEM206 currents in Na2SO4 were too small for Erev determination. Currents were elicited by voltage ramps as in Figure 6C. (E) Reversal potential Erev with extracellular NaCl (***, p<0.001 vs. WT; one-way ANOVA, Bonferroni correction). (F, G) Shift of reversal potentials (ΔErev) with extracellular NaI (F) or Na2SO4 (G) relative to that measured with NaCl (NA, not applicable (currents not significantly above background or Erev not stable over time (I307C)). (H) Current densities at +80 mV with extracellular Na2SO4 at pH 5.25. *, p<0.033 and ***, p<0.001 vs. WT; one-way ANOVA, Bonferroni correction. No statistical analysis was performed on the data in (G).


Role of TMEM206/ASOR channels in acidotoxicity

ASOR was suggested (Sato-Numata et al., 2014; Wang et al., 2007) to play a role in acidotoxicity because DIDS and phloretin, compounds inhibiting ASOR but also several other channels or transporters, reduced acid-induced necrotic cell death of HeLa cells (Wang et al., 2007) and cultured cortical neurons (Sato-Numata et al., 2014). We exposed both WT HEK cells, and two separately derived TMEM206−/− clones, for 2 hr to acidic medium (pHo 4.5) in the absence and presence of PS. Cell death was assessed by propidium iodide staining and normalized to the total number of cells as determined by Hoechst 33342 labeling. Indeed, disruption of TMEM206 partially protected against acid-induced cell death (Figure 8A,B). A roughly similar protection was seen with PS in WT but not in TMEM206−/− cells (Figure 8A,B), suggesting that the effect of PS on cell viability results from its ability to block ASOR (Drews et al., 2014). In conclusion, ASOR/TMEM206 channels enhance acidotoxicity.

Figure 8 with 1 supplement see all
Role of TMEM206/ASOR channels in acid-induced cell death and volume regulation.

(A) Representative pictures of WT and TMEM206−/− HEK293 cells (clone 2E5) double stained with propidium iodide (PI, red) and Hoechst 33342 (blue) after incubation for 2 hr with control (pH 7.4) or acidic (pH 4.5) solution, also containing the potent inhibitor of TMEM206 pregnenolone sulfate (PS) at 100 µM or not. In this assay, dead cells are stained with PI, membrane impermeant, whereas Hoechst 33342, which is permeant, stains nuclei of all cells. Scale bars: 100 µm (B) Quantification of PI positive cells over the total number of cells, determined by Hoechst staining, in WT and two independent TMEM206−/− HEK clones generated with different guide RNAs (g2, g3). For quantification, 10x objective microscopic fields were randomly chosen. Bars represent mean ± SEM. Data were acquired in three independent experiments and analyzed by using one-way ANOVA with post hoc multiple comparisons using the Tukey’s test: ***p<0.001 (C,D) Influence of TMEM206 ablation (C) or block by 100 µM pregnenolone sulfate (PS) (D) on acid-induced cell volume changes of HEK293 cells as monitored by calcein fluorescence. Mean of eight measurements; error range, SEM. Similar results were obtained in three experiments, and similar effects were observed with HeLa cells (Figure 8—figure supplement 1).


This form of cell death had been attributed to cell swelling caused by osmotic gradients generated by ASOR-mediated Cl- influx (Sato-Numata et al., 2014; Wang et al., 2007). When exposed to pHo 4.2, both WT and TMEM206−/− HEK cells increased their volume within the first three minutes as indicated by increased calcein fluorescence (Figure 8C). Agreeing with the hypothesis (Sato-Numata et al., 2014; Wang et al., 2007) that the parallel opening of ASOR and ASICs (acid-sensitive Na+-channels; Gründer and Pusch, 2015; Waldmann et al., 1997) leads to an electrically coupled influx of osmotically active Na+ and Cl-, WT cells swelled faster than TMEM206−/− cells. Whereas WT cells subsequently decreased their volume, TMEM206−/− cells failed to shrink (Figure 8C). Likewise, the ASOR inhibitor pregnenolone sulfate (100 µM) (Drews et al., 2014) slowed the initial swelling and inhibited the subsequent shrinkage (Figure 8D). Similar effects of ASOR disruption or block were observed with HeLa cells (Figure 8—figure supplement 1). Hence, contrasting with the conclusion of Wang et al. (2007), our data suggest that the role of ASOR/TMEM206 in acidotoxicity cannot be explained by a sustained volume increase of acid-exposed cells.


We have identified TMEM206 as essential, pore-forming subunit of ASOR, a widely expressed acid-sensitive outwardly rectifying anion channel. TMEM206 lacks homologous proteins within any given species. Both transmembrane domains of TMEM206, most importantly TM2, likely participate in forming its anion-selective pore. TMEM206/ASOR channels are apparently present in all vertebrates and share activation by markedly acidic pH as prominent feature. TMEM206/ASOR channels play a role in pathologies associated with a marked decrease in extracellular pH. Although their wide expression pattern across tissues and vertebrates suggests important physiological functions for all cells, these roles remain to be determined. The identification of TMEM206 as ASOR channel-forming protein in this work and in a recently published independent study by Qiu and coworkers (Yang et al., 2019) is an essential step forward towards the elucidation of these roles.

TMEM206 fully constitutes ASOR

The dissimilar biophysical characteristics of currents elicited by TMEM206 orthologs and the changes observed with mutants demonstrate that TMEM206 proteins constitute the pore of ASOR channels. With only two exceptions, all cysteine-substituted channels gave currents. However, their amplitudes were reduced by several mutations which were distributed along the length of either TM1 or TM2 (Figure 6—figure supplement 2). Whereas this result only shows that TMEM206 is (somehow) important for ASOR currents, the observed functional effects of MTSES on various cysteine mutants additionally indicate that the respective cysteines are accessible from the aqueous phase. If such residues are located in a membrane-embedded region, they are probably close to, or line, the pore. More convincing evidence that TMEM206 proteins form the channel comes from mutations that change intrinsic channel properties such as rectification or ion selectivity (a property of the pore). Several mutations, both at the extracellular end of TM1 and at various positions along TM2, moderately changed ASOR’s I- permeability. Moreover, whereas WT hTMEM206 lacked measurable currents with extracellular Na2SO4, TMEM206 from anole and zebrafish, and a surprisingly large number of mutants, displayed robust currents with extracellular sulfate. These mutations may markedly increase the channel’s SO42- (or HSO4-) permeability, or relieve a channel block that was indicated by the absence of inward currents (efflux of Cl-) in the presence of extracellular sulfate. The most striking evidence for TMEM206 forming ASOR’s pore comes from the loss of anion selectivity with the L315D mutant. The ~22 mV positive shift of the reversal potential indicates a marked increase in cation conductance (Erev for Na+ or H+ are >+120 mV and +113 mV, respectively) that might be due to electrostatic interactions of the newly introduced negatively charged aspartate with the permeant ion. Our data suggest that the whole length of TM2, with a participation of the outer end of TM1, lines the pore of ASOR. This is reminiscent of crystallographically resolved pore structures of ASIC channels with which ASOR shares the transmembrane topology, but no obvious sequence homology. Intriguingly, ASICs and other ENaC/Deg channels display a glycine residue (in the ‘GxS’ motif) in TM2 that plays an important role in determining pore properties and gating (Baconguis et al., 2014; Li et al., 2011). Whether G212, which is located at a similar distances from the ends of TM2, plays a comparable role must await determination of TMEM206/ASOR channel structures.

Heterologous expression of all tested vertebrate orthologs led to large acid-induced currents. As shown with human TMEM206, biophysical properties of these currents did not differ significantly from native ICl,H. Hence completely functional ASOR channels may only require TMEM206. However, our results cannot strictly exclude that TMEM206 lines the pore together with other, unknown proteins. This scenario would require that such proteins are produced in excess endogenously since overexpression of hTMEM206 or its orthologs elicited currents > 10 fold larger than native ICl,H. It further requires that such a putative additional pore-forming protein from HEK cells is able to associate with all the vertebrate orthologs tested. If ASOR’s pore is exclusively constituted by TMEM206 proteins that cross the membrane only twice, TMEM206 must form at least a dimer to build a protein-enclosed pore. ASICs and other members of the DEG/ENaC channel family, which display a transmembrane topology similar to TMEM206, form trimeric homo- or heteromeric channels (Jasti et al., 2007). Likewise ASOR channels might be formed by TMEM206 trimers.

Role of acid-activated chloride current

With the exception of the hemichordate TMEM206, which shows marked sequence divergence and was retained in the ER, all tested TMEM206 proteins gave strongly outwardly rectifying Cl- currents that were activated by extracellular acidification in excess of ~pHo 6. The strong rectification might be physiologically irrelevant since all orthologs gave currents also at negative voltages and because inside-positive voltages are rarely reached in vivo, in particular in non-excitable cells. By contrast, the conserved activation by acidic pHo appears crucial and poses the question where and when such pH values might be reached. Mammalian ASORs require slightly less acidic pH for activation at physiological temperatures (37°C) (Sato-Numata et al., 2013; Sato-Numata et al., 2014), but this consideration seems irrelevant for the reptile and fish orthologs.

Only a few cell types in the mammalian organism are physiologically exposed to an extracellular pH that is more acidic than pH 6.0. These include cells in the stomach and duodenum, renal medullary collecting duct (Bengele et al., 1983) and acid-secreting osteoclasts and macrophages (Silver et al., 1988), but it is unclear whether ASOR is expressed in the relevant membranes of these polarized cells. Moreover, it is unlikely that an important function in a small subset of cells has resulted in an almost ubiquitous expression of ASOR during evolution. On the other hand, intracellular compartments such as endosomes and lysosomes, which can be acidified down to pH 4.5, would provide an ideal environment for ASOR activity. Here ASOR might facilitate luminal acidification by shunting currents of the proton-ATPase as described for endosomal CLC Cl- transporters (Günther et al., 1998; Jentsch, 2007; Piwon et al., 2000). Interestingly, vesicular CLCs also show strong outward-rectification, but unlike ASOR are 2Cl-/H+-exchangers that are inhibited by luminal (or extracellular) acidification (Jentsch, 2007; Jentsch and Pusch, 2018; Scheel et al., 2005). Hence presence of both ASOR and CLCs on endolysosomes might allow for a differential regulation of vesicular membrane voltage and ion concentrations. However, ClC-7 is believed to be the main Cl- transporter of lysosomes (Graves et al., 2008; Kornak et al., 2001) and immunocytochemistry showed prominent plasma membrane expression of all examined vertebrate ASOR orthologs. Although the variable cytoplasmic labeling observed upon TMEM206 overexpression may have resulted from overexpression and showed no obvious co-localization with the lysosomal marker lamp-1 in preliminary experiments, we feel that a more thorough investigation of an additional localization of ASOR in intracellular compartments may be warranted.

When speculating about biological roles of ASOR it is instructive to compare it to acid-sensitive ASIC channels (Waldmann et al., 1997; Wemmie et al., 2013). Besides being cation channels, ASICs differ from ASOR by their larger molecular diversity (four different subunits can assemble to various homo- and hetero-trimers), rapid inactivation and the degree of pH-sensitivity. Depending on the isoform, their pH50 for activation ranges between ~4.8 and ~6.6 (Gründer and Pusch, 2015; Wemmie et al., 2013), values that are up to more than 1 pH unit more alkaline than that of ASOR (pH50 ~5.3). Hence several physiological functions of ASICs are unlikely to have equivalents with ASOR. For instance, postsynaptic ASICs may enhance excitatory synaptic transmission by opening in response to a drop in pH in the synaptic cleft that is caused by exocytosis of the acidic contents of synaptic vesicles (Chu and Xiong, 2012; Gründer and Pusch, 2015; Huang et al., 2015). Even if ASOR would be expressed postsynaptically, the estimated drop in pHo (Gründer and Pusch, 2015) seems to be too small for its activation. ASICs also have roles in peripheral pain sensation (Waldmann et al., 1997; Wemmie et al., 2013). If expressed on appropriate sensory neurons, such a function is imaginable also for ASOR because the high intracellular Cl- concentration of peripheral neurons would allow for a depolarizing Cl--efflux (Funk et al., 2008). Strikingly, naked mole-rats are insensitive to acid-induced pain, but their ASIC channels show normal pH-sensitivity (Smith et al., 2011). We likewise found here that TMEM206/ASOR channels of that species display normal pH-sensitivity. The pH-insensitivity of mole-rats may rather be due to a changed pH-sensitivity of the Na+-channel Nav1.7 (Eigenbrod et al., 2019; Smith et al., 2011).

Of note, ASIC channels are involved in various neurological pathologies that are associated with acidic pHo (Huang et al., 2015; Wemmie et al., 2013), including stroke (Xiong et al., 2004) and multiple sclerosis (Friese et al., 2007). Likewise, based on protective effects of non-specific ASOR inhibitors, Okada and colleagues proposed that ASOR plays a role in acid-induced cell death of epithelial cells (Wang et al., 2007) and neurons (Sato-Numata et al., 2014). Using TMEM206−/− cells, we now ascertained that genetic ablation of TMEM206/ASOR channels partially protects cells from cell death provoked by strongly acidic pHo (4.5). Interestingly, when compared to mice, cortical neurons of naked mole rats are resistant to acid-induced cell death (at pH 5.0) (Husson and Smith, 2018). Since neither the pH-response of ASICs (Smith et al., 2011) nor of ASOR (this work) is changed in that species, the explanation for this resistance may be due to channel expression levels or other factors.

It has been proposed (Wang et al., 2007) that ASOR-dependent, acid-induced cell death results from sustained cell swelling owed to the parallel influx of Na+ and Cl- through acid-activated ASIC and ASOR channels, respectively. Whereas in our experiments WT cells initially swelled faster than TMEM206−/− cells, they subsequently recovered their volume and even shrank. Initial swelling may indeed be owed to the opening of both ASIC and ASOR, with ASIC-mediated Na+-influx mediating the depolarization required for passive Cl- influx. In contrast to ASOR, however, ASIC channels inactivate (Gründer and Pusch, 2015), leading to a reversal of the Cl- electrochemical potential, passive efflux of Cl- through ASOR, and subsequent osmotic cell shrinkage. Although there is now little doubt that TMEM206/ASOR channels foster acid-induced cell death, the underlying signal transduction cascade likely is complex.

Recently, while the present work was being completed, Yang et al. reported on their independent identification and characterization of TMEM206 as integral component of the ASOR channel (which the authors renamed PAC for Proton-Activated Channel) (Yang et al., 2019). They likewise reported protective effects of TMEM206 disruption on acid-induced cell death of HEK cells or cultured primary cortical neurons. Intriguingly, these authors showed that Tmem206−/− mice showed smaller stroke areas in a middle cerebral artery occlusion model (Yang et al., 2019), although the previously reported decrease of pHo in the stroke area (down to ~6.4) (Nedergaard et al., 1991) is slightly above the value needed for ASOR activation. In a cysteine modification scan restricted to the extracellular half of TM2, they also found that reaction of I307C with MTSES reduces ASOR currents, and showed that the I307A mutant displayed moderately decreased iodide permeability. These results agree with our conclusion that the entire TM2 and the extracellular end of TM1 line ASOR’s pore in an oligomeric complex.


We have identified TMEM206 as essential subunit of the acid-sensitive outwardly rectifying anion channel ASOR. TMEM206 proteins constitute the pore of ASOR, probably in a homo-oligomeric complex. TMEM206, which lacks paralogs in any given species, defines a structurally novel class of ion channels. TMEM206/ASOR channels are involved in acid-induced cell death, but this role in pathology cannot explain its wide and possibly ubiquitous expression across tissues and vertebrate species. Its molecular identification in this work and in the parallel study of Yang et al. (2019) is an important step to identify its physiological roles and to mechanistically understand the diverse ways by which anion-selective channels can be formed.

Materials and methods

Key resources table
Reagent type
(species) or resource
DesignationSource or referenceIdentifiersAdditional
Cell line (Homo sapiens)HEK293 WTDeutsche Sammlung von Mikroorganismen und Zellkulturen, GermanyACC No. 305; RRID: CVCL_0045
Cell line (Homo sapiens)HEK293 TMEM206-/-this papergenerated from WT HEK293,
different clones (Table 1)
Cell line (Homo sapiens)HeLa WTDeutsche Sammlung von Mikroorganismen und Zellkulturen, GermanyACC No. 57; RRID: CVCL_0030
Cell line (Homo sapiens)HeLa TRExThermo FisherCat. No. R71407; RRID: CVCL_D587
Cell line (Homo sapiens)HeLa-E2GFP-2A-FaNaCthis papersee below for FaNaC/E2GFPgenerated from HeLa TREx, clone E2F-5
Cell line (Homo sapiens)HeLa TMEM206-/-this papergenerated from HeLa-E2GFP-2A-FaNaC, different clones (Table 1)
Transfected construct (S. pyogenes)pSpCas9(BB)−2A-GFP (PX458)PMID: 24157548RRID: Addgene_48138
Gene (Homo sapiens)Human TMEM206this paperRefSeq: NM_018252.2; UniProtKB: Q9H813Cloned from human brain cDNA library (Clontech)
Gene (Heterocephalus glaber)Naked mole-rat TMEM206this paperRefSeq: XM_004853543.3; UniProtKB: A0A0N8ETT8human codon-optimized, synthesized by Life Technologies
Gene (Gallus gallus)Chicken TMEM206this paperRefSeq: XM_419431.6; UniProtKB: A0A1D5PSZ0human codon-optimized, synthesized by Life Technologies
Gene (Anolis carolinensis)Green anole TMEM206this paperRefSeq: XM_003216011.3; UniProtKB: G1KFB8human codon-optimized,
synthesized by Life Technologies
Gene (Danio rerio)Zebrafish TMEM206this paperRefSeq: NM_001291762.1; UniProtKB: X1WG42human codon-optimized, synthesized by Life Technologies
Gene (Saccoglossus kowalevskii)Acorn worm TMEM206-likethis paperRefSeq: XM_006811230.1human codon-optimized, synthesized by Life Technologies
Gene (Cornu aspersum)FaNaCPMID: 7501021; PMID: 16034422GenBank: X92113.1; UniProtKB: Q25011
Gene (Aequorea victoria)E2GFP (GFP S65T/T203Y)PMID: 17434942, PMID: 20581829subcloned from ClopHensor (Addgene #25938)
Antibodyanti-GFP (chicken polyclonal)Aves LabCat. No. GFP-1020; RRID: AB_10000240(1/1,000)
Antibodyanti-HA (rabbit monoclonal)Cell SignalingCat. No. 3724;RRID: AB_1549585(1/1,000)
Antibodyanti-GFP (rabbit polyclonal)Thermo FisherCat. No. A-11122; RRID: AB_221569(1/1,000)
Antibodyanti-Na/K-ATPase (mouse monoclonal)MilliporeCat. No. 05–369; RRID: AB_309699(1/10,000)
Antibodyanti-rabbit AlexaFluor 633 (goat polyclonal)InvitrogenCat. No. A21071; RRID: AB_2535732(1/2,000)
Antibodyanti-chicken HRP (rabbit polyclonal)SigmaCat. No. A-9792; RRID: AB_258473(1/10,000)
Antibodyanti-mouse HRP Affinipure (goat polyclonal)Jackson ImmunoResearchCat. No. 115-035-003; RRID: AB_10015289(1/10,000)
Sequence-based reagentDharmacon
ON-TARGETplus human siRNA library
Horizon DiscoveryCat. No. G-105005-E2-025arrayed in 384-well plates
Chemical compound, drugFMRFamide (H-Phe-Met-Arg-Phe-NH₂)BachemCat. No. 40015665 mM stock in ddH2O, stored at −20°C for up to 6 months
Chemical compound, drugMTSES (2-Sulfonatoethyl methanethiosulfonate)BiotiumCat. No. 91020250 mM stock in ddH2O, freshly prepared, kept on ice for up to 4 hr
Chemical compound, drugPregnenolone sulfate (PS)SigmaCat. No. P16250 mM stock in DMSO (stored at −20°C for up to 6 months) for electrophysiology
experiments or freshly diluted in PBS for cell death analysis
Chemical compound, drug4,4′-Diisothiocyanatostilbene-2,2′-disulfonic acid (DIDS)SigmaCat. No. D3514100 mM stock in DMSO, stored at −20°C for up to 6 months
Chemical compound, drugNiflumic acid (NFA)SigmaCat. No. N0630300 mM stock in DMSO, stored at −20°C for up to 6 months
Chemical compound, drugHoechst 33342ImmunoChemistry TechnologiesCat. No. ICT-639
Chemical compound, drugPropidium iodideThermo FisherCat. No. P3566
Chemical compound, drugCalcein AMThermo FisherCat. No. 65-0853-3910 mM stock in DMSO, stored at −20°C for up to 6 months

HeLa-E2GFP-2A-FaNaC Cell Line used in the siRNA Screen

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The T-REx system (Life Technologies) was used to generate a stable HeLa cell line inducibly co-expressing the halide-sensitive E2GFP variant (GFP S65T/T203Y) (Arosio et al., 2007) and the FMRFamide-gated cation channel FaNaC (Lingueglia et al., 1995) (UniProtKB Q25011). A pcDNA5/FRT/TO-based plasmid containing the cDNAs encoding E2GFP and FaNaC, separated by a self-cleaving 2A peptide was generated for this purpose. Resulting clones were selected using 200 µg/ml hygromycin B (Santa Cruz) and 4 µg/ml blasticidin (InvivoGen). Monoclonal cell lines were tested for robust expression of E2GFP and FaNaC by fluorescence microscopy and patch clamp electrophysiology, respectively. Clone E2F-5 was chosen for the genome-wide screen. Cells were kept in DMEM supplemented with 10% tetracycline-free Hyclone FCS (Fisher Scientific) and the above-mentioned antibiotics.

Genome-wide siRNA screen

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The genome-wide siRNA screen was performed at the FMP Screening Unit using the Dharmacon ON-TARGETplus human siRNA library (Horizon Discovery) arrayed in 66 384-well plates, targeting 18090 genes by four pooled siRNAs each. The screen was performed three times.

For siRNA transfection, on a Freedom EVO 200 workstation (Tecan) 4 μl of a 500 nM library-siRNA-OptiMEM solution was mixed in each well of the 384-well assay-plate with 0.125 μl Lipofectamine RNAiMAX transfection reagent (Life Technologies) previously diluted in 5.875 μl OptiMEM (Life Technologies). Subsequently, 3,000 cells/well in phenol red-free DMEM (PAN Biotech) were seeded onto the pre-dispensed transfection mixture using an EL406TM dispenser (BioTek) resulting in a final concentration of 50 nM siRNA SMARTpool in a total volume of 40 μl per well. To induce E2GFP-2A-FaNaC expression after 24 hr, 10 µl phenol red-free DMEM containing 5 μg/ml doxycycline were added resulting in a final concentration of 1 μg/ml doxycycline.

The quenching assay was performed 72 hr post-transfection. First, using an EL406 washer dispenser (BioTek), cells were washed three times with 120 µl/well of a bath solution containing (in mM): 145 NaCl, 5 KCl, 1 MgCl2, 2 CaCl2, 10 glucose, 10 HEPES, pH 7.4 with NaOH, 320 mOsm/kg. Subsequently, wells were aspirated to 10 µl residual volume. The plates were transferred into the FLIPR Tetra High Throughput Cellular Screening System (Molecular Devices) for measurements. All wells of the plate were simultaneously illuminated at λ = 470–549 nm with an exposure time of 0.53 s and E2GFP-fluorescence was measured at λ = 515–575 nm. Measurements were taken in intervals of 1 s for 300 s. After recording the baseline fluorescence for 10 s, parallel pipetting added 25 μl acidic iodide-containing solution with the following composition (in mM): 145 NaCI, 5 KCl, 1 MgCl2, 2 CaCl2, 10 glucose, 5 Na3citrate and 30 µM FMRFamide (H-Phe-Met-Arg-Phe-NH2 acetate salt, Bachem), pH 4.83, 320 mOsm/kg. The mixture of this solution with remaining bath solution resulted in approximately 100 mM iodide, 20 µM FMRFamide and pH 5. Control wells received the same solution without FMRFamide.

Analysis and processing of data obtained in the genome-wide screen were performed with the KNIME Analytics Platform (KNIME). The maximal slope of fluorescence change was determined by linear regression of 10 points in a sliding window between 20 s and 100 s. Slope was normalized to the averaged baseline and background fluorescence.

For plate-wise normalization a Z-score was calculated from the normalized slope value as follows:

Z=xi-x~ MADx

where xi is the normalized slope from well i, x~ is the median and MADx the median absolute deviation of all candidate wells in the plate.

The median of the three replicate Z-scores per target gene was used to rank the data. As a measure of cell viability, an additional Z-score was calculated from the baseline fluorescence. We filtered out all candidates in which the median Baseline START Z-score was lower than −0.5, indicating a significantly reduced cell number.


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Cells were plated onto poly-L-lysine-coated coverslips and transfected using the Xfect (Clontech) transfection reagent. Whole-cell patch-clamp recordings were performed 1 day after transfection using an EPC-10 USB patch-clamp amplifier and PatchMaster software (HEKA Elektronik). All experiments were performed at 20–22°C. Patch pipettes had a resistance of 1–3 MΩ in NaCl/CsCl-based solutions. The holding potential was −30 mV. Cells with a series resistance above 8 MΩ were discarded, and series resistance was not compensated for. Currents were sampled at 1 kHz and low-pass filtered at 10 kHz. In some cases, a notch filter was applied to current traces post-recording to minimize line noise.

The standard pipette solution contained (in mM): 150 CsCl, 10 EGTA, 10 HEPES, 5 MgCl2, pH 7.2 with CsOH (320 mOsm/kg). The standard pH 7.4 bath solution in which cells were kept before activation of acid-induced currents contained (in mM): 145 NaCl, 5 KCl, 1 MgCl2, 2 CaCl2, 10 glucose, 10 HEPES, pH 7.4 with NaOH (320 mOsm/kg). Solutions with a pH between 4.0 and 5.25 were identical in composition, but buffered with 5 mM Na3citrate instead of HEPES. pH of these solutions was adjusted using citrate. Likewise, solutions with a pH between 5.5 and 6.0 were buffered with 10 mM MES and adjusted with NaOH. To determine anion selectivities (Figure 4A), NaCl in the pH 4.8 bath solution was replaced by equimolar amounts of NaBr, NaI, NaNO3 and NaSCN. The control bath solution for the ion selectivity experiments shown in Figure 5E–I and Figure 7 contained (in mM) 150 NaCl, 2 Ca(OH)2, 1 EGTA, five citric acid, pH 5.25 with NMDG-OH (320 mOsm/kg). To determine relative permeabilities, NaCl was replaced by an equimolar amount of NaI, or with 100 mM Na2SO4. In these experiments the pipette solution contained (in mM): 140 NMDG-Cl, 10 EGTA, 10 HEPES, 5 MgCl2, pH 7.2 with NMDG-OH (320 mOsm/kg). Osmolarities of all solutions were assessed with an Osmomat 030 freezing point osmometer (Gonotec) and adjusted using mannitol.

MTSES was purchased from Biotium and stored at −20°C as powder. MTSES stock solutions (250 mM in water) were freshly prepared on each day and kept on ice until final dilution in recording solutions right before experiments.

The standard protocol for measuring the time course of acid activated currents, applied every 4 s after membrane rupture, consisted of a 100 ms step to −80 mV followed by a 500 ms ramp from −80 to +80 mV. The read-out for current amplitudes was the current at −80 mV and +80 mV normalized to cell capacitance (current density, I/C). The voltage protocol, applied after complete activation of ASOR, consisted of 1 s steps starting from −80 mV to +80 mV in 20 mV intervals preceded and followed by a 0.5 s step to −80 mV every 4 s.

An agar bridge was used in all electrophysiological experiments. Solution was exchanged in the entire bath chamber using a custom gravity-fed perfusion system under TTL control, achieving full exchange in <5 s. Liquid junction potentials (LJPs) were only considered in ion selectivity experiments (Figures 5 and 7). LJPs were measured experimentally for all solutions used in these experiments with reference to NMDG-Cl pipette solution according to Neher (1992) and corrected for after data acquisition. The measured values (mean ± SD) were: 4.5 ± 0.3 mV (NaCl, n = 4), 4.3 ± 0.3 mV (NaI, n = 4), and 9.5 ± 0.1 mV (Na2SO4, n = 4).

Molecular biology

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Human TMEM206 was cloned from cDNA obtained from a human brain cDNA library (Clontech) using the following primers:



HindIII and BamHI restriction sites on the primers were used to clone the PCR product into pcDNA3.1(+) (Invitrogen). Sequencing of the entire ORF confirmed the sequence was identical to NCBI RefSeq NM_018252.2. Subsequently, the cDNA was subcloned into other vectors such as pEGFP-N1/C1 using PCR-based strategies. Point mutations were introduced using the QuikChange Kit (Agilent). All clones were verified by sequencing the entire ORF. TMEM206 ortholog cDNA clones (human codon-optimized) were synthesized by Life Technologies. The cDNAs were subcloned into pEGFP-C1 for fusion of an N-terminal GFP tag.

Cell culture

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HEK293 and HeLa cells were maintained in DMEM (PAN Biotech) supplemented with 10% FBS (PAN Biotech) and 1% penicillin/streptomycin at 37°C and 5% CO2. Cells were regularly tested for mycoplasma contamination.

Generation of monoclonal knockout cell lines

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Cell lines with disruptions in the TMEM206 gene were generated using CRISPR-Cas9 as described previously (Voss et al., 2014). Briefly, target sgRNAs were cloned into the pSpCas9(BB)−2A-GFP (PX458, gift from Feng Zhang, MIT) vector and transfected into HEK293 and HeLa cells. 2–5 days post-transfection single GFP-positive cells were FACS-sorted into 96-well plates containing pre-conditioned DMEM medium. Monoclonal cell lines were raised and tested for sequence alterations using target-site-specific PCR on genomic DNA followed by Sanger-sequencing. sgRNA sequences, genetic modifications and resulting protein mutations are listed for each clone in Table 1.


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To determine the transmembrane topology of TMEM206, TMEM206−/− HeLa cells (clone 3F8) were transfected with plasmids encoding GFP-TMEM206, TMEM206-GFP or TMEM206 containing a HA tag (YPYDVPDYA) after residue 271 using Lipofectamine 2000. 24 hr after transfection, cells were fixed with 2% PFA for 10 min and permeabilized with 0.1% Triton X-100 as indicated. Cells were incubated with chicken anti-GFP (1/1000, Aves Lab) or rabbit anti-HA (1/1000, Cell Signaling) for 2 hr and subsequently with secondary antibodies coupled to AlexaFluor633 for 1 hr. For the non-permeabilized conditions, no Triton X-100 was added to the antibody solutions.

Images were acquired with a confocal microscope with a 63x NA1.4 oil-immersion lens (LSM880, Zeiss).

Deglycosylation and western blot

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To determine glycosylation of TMEM206, TMEM206−/− HEK cells (clone 2E5) were transfected with plasmids coding for GFP-TMEM206 or mutants of putative glycosylation sites (GFP-TMEM206 N148A, N155A, N162A, N190A) using polyethylenimine (PEI). One day after transfection, cells were scrapped in homogenization buffer containing (in mM): 140 NaCl, 20 Tris HCl pH 7.4, 2 EDTA, 4 Pefabloc (Roth) and Complete proteinase inhibitor cocktail (Roche). Cell debris were removed by centrifugation at 1000 g for 10 min and the supernatant was ultracentrifuged at 269,000 g for 30 min. Pellets containing the membrane fractions were resuspended in lysis buffer containing (in mM): 140 NaCl, 50 Tris HCl pH 6.8, 0.05 EDTA, 1% SDS, 1% Triton X-100, 2 EDTA, 4 Pefabloc (Roth) and Complete proteinase inhibitor cocktail (Roche). Protein concentration of the membrane fractions was determined by BCA.

40 μg of membrane protein were denaturated by adding 2 μl of denaturation buffer (New England Biolabs), for a final volume of 20 μl, and heating the reaction at 55°C for 15 min. 4 μl of 10xG7 Buffer (NEB), 4 μl of 10% NP-40 (NEB) and 8 μl of PNGase F (NEB) were added, for a total volume of 40 μl. After 2 hr incubation at 37°C, the deglycosylation reaction was terminated by adding 10 μl of Lämmli sample buffer. Samples were separated by SDS-PAGE and analyzed by Western blot using rabbit anti-GFP antibody (1/1000, Thermo Fisher). Na/K-ATPase was used as loading control and detected using a mouse antibody (1/10,000, Millipore).

Acid-Induced cell death assay

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Acid-induced cell death of WT and TMEM206−/− HEK293 cells (clones 2E5 and 3D9) was assessed by double staining with Hoechst 33342 and propidium iodide (PI). WT and KO cells were incubated for 2 hr at 37°C with solution at pH 7.4 (HEPES) or 4.5 (citrate, see Electrophysiology). Experimental solutions were subsequently replaced with pH 7.4 solution containing Hoechst 33342 (1 µg/ml, ImmunoChemistry Technologies) and PI (5 µg/ml, Thermo Fischer) and cells were incubated for 15 min at 37°C. After washing, cells were fixed and analyzed using a confocal microscope (LSM880, Zeiss). For quantification, several 10x microscopic fields were randomly chosen. Percentage of PI positive cells over the total number of cells, identified with Hoechst 33342, was determined using Fiji.

Cell volume measurements

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Cell volume was measured semi-quantitatively using the calcein fluorescence method (Capó-Aponte et al., 2005). HEK293 cells were plated 2 days before measurements in a 96-well-plate at a density of 45,000 cells per well, HeLa cells were plated 1 day before measurements at a density of 35,000 cells per well. Cells were loaded with 10 μM calcein-AM (eBioscience) in DMEM for 1 hr at 37°C. Subsequently, cells were washed 3 times with 100 μl of a pH 7.4 (HEPES, see Electrophysiology) bath solution. After washing, each well contained 50 μl bath solution. Following a 5 min incubation period the plate was transferred into Safire² Multimode Microplate Reader (Tecan) and fluorescence measurements at λ = 495 nm were initiated. After baseline recording for 30 s, 125 μl of a pH 4.0 (citrate, see Electrophysiology) solution, or pH 7.4 bath solution were added to the wells resulting in a final pH of 4.2 or 7.4 respectively. Calcein fluorescence was measured every 10 s for 10 min. Wells containing cells of the respective cell line not loaded with calcein-AM (but otherwise treated equally) were used for background subtraction, and fluorescence values were normalized to t = 0 s (after the pipetting procedure).

Data availability

Raw data are in part presented in the manuscript (e.g. IHC, Western, clamp traces), and as source data files where data points (such as current densities, ratios of permeability etc) have been extracted from original electrophysiological recordings.


Decision letter

  1. Reinhard Jahn
    Reviewing Editor; Max Planck Institute for Biophysical Chemistry, Germany
  2. Olga Boudker
    Senior Editor; Weill Cornell Medicine, United States
  3. Reinhard Jahn
    Reviewer; Max Planck Institute for Biophysical Chemistry, Germany
  4. Cecilia M Canessa
    Reviewer; Tsinghua University, China
  5. Johannes Oberwinkler
    Reviewer; Philipps-Universität Marburg, Germany

In the interests of transparency, eLife includes the editorial decision letter and accompanying author responses. A lightly edited version of the letter sent to the authors after peer review is shown, indicating the most substantive concerns; minor comments are not usually included.

Thank you for submitting your article "Identification of TMEM206 proteins as pore of ASOR acid-sensitive chloride channels" for consideration by eLife. Your article has been reviewed by three peer reviewers, including Reinhard Jahn as the Reviewing Editor and Reviewer #1, and the evaluation has been overseen by Olga Boudker as the Senior Editor. The following individuals involved in review of your submission have agreed to reveal their identity: Cecilia M Canessa (Reviewer #2); Johannes Oberwinkler (Reviewer #3).

The reviewers have discussed the reviews with one another and the Reviewing Editor has drafted this decision to help you prepare a revised submission.


Ullrich et al. present cloning and characterization of a human proton-activated chloride channel ubiquitously expressed in mammalian cells and other distant vertebrates that correspond to the protein TMEM206. This channel previously known as acid-sensitive outward rectifying chloride channel (ASOR) was also recently cloned by Yang et al. who named it proton activated chloride channel or PAC. Both studies report almost identical results with Yang also publishing a knockout mouse – which was viable and did not exhibit apparent abnormalities – but was partially protected form ischemic injury of the brain.

The work of Ullrich et al. is convincing, of high quality, and the manuscript is written in an excellent manner. Most reassuring is that both groups obtained the same findings, only differing in the response of cells exposed to low pH that first swell and later recover their volume in the presence of TMEM206.

Essential revisions:

All three reviewers were enthusiastic about the work, and all recommended acceptance of the manuscript. There are no essential revisions but some minor issues that one of the reviewers would like the authors to consider before submitting the final version.

Reviewer #3:

Minor points:

I personally like rather verbose Materials and methods sections, and I therefore would have wished for more information on a few occasions, specifically:

- How was the Z-score calculated, and what do the authors mean by "robust statistics"?

- How was the solution exchanged in electrophysiological experiments, and did the precise way of exchanging solutions (potentially) have an impact on liquid junction potentials? Did the authors use an agar-bridge for the reference electrode?

- How was the liquid junction potential between different solution measured? As this is not an entirely trivial procedure (with potentially different outcomes depending on the method used), it would be desirable that the authors shared the values measured, especially for those experiments where anions were replaced completely.

Normally, I would consider not employing series resistance compensation an avoidable mistake when measuring currents of several nano-amperes with a series resistance of more than 5 MΩ. In this manuscript, the choice not to use this technique has no impact on the conclusions. It may, however, have affected the shape of some of the I/V curves (for example, the linearity of the curve in Figure 4H is perhaps due to voltage-clamp errors). Maybe the authors want to point this out in an appropriate place.

The authors point out in the Introduction that the name "PAORAC" provides a better description compared to "ASOR" and in addition, it is actually the older name. This, of course, is flattering to me as I coined the term PAORAC many years back. Maybe, the authors want to consider to use the term "PAORAC" also in the title, possibly together with the newer "ASOR".

In Figure 7E, it is hard to identify that the "three stars" of the L315D mutation belong to this panel, as they are plotted between panel E and F. In Figure 7G and H, why is there no statistics reported?


Author response

Reviewer #3:

Minor points:

I personally like rather verbose Materials and methods sections, and I therefore would have wished for more information on a few occasions, specifically:

- How was the Z-score calculated, and what do the authors mean by "robust statistics"?

“Robust statistics” denotes the use of median and MAD instead of mean and SD, but we agree that this is somewhat ambiguous. To clearly explain how the Z-score was calculated, we now added a formula (subsection “Genome-wide siRNA Screen”, fourth paragraph).

- How was the solution exchanged in electrophysiological experiments, and did the precise way of exchanging solutions (potentially) have an impact on liquid junction potentials? Did the authors use an agar-bridge for the reference electrode?

- How was the liquid junction potential between different solution measured? As this is not an entirely trivial procedure (with potentially different outcomes depending on the method used), it would be desirable that the authors shared the values measured, especially for those experiments where anions were replaced completely.

We added more detailed descriptions of these procedures, as well as the values measured for LJPs to the Materiuals and methods section (subsection “Electrophysiology”, last paragraph).

Normally, I would consider not employing series resistance compensation an avoidable mistake when measuring currents of several nano-amperes with a series resistance of more than 5 MΩ. In this manuscript, the choice not to use this technique has no impact on the conclusions. It may, however, have affected the shape of some of the I/V curves (for example, the linearity of the curve in Figure 4H is perhaps due to voltage-clamp errors). Maybe the authors want to point this out in an appropriate place.

We thank the reviewer for pointing out this minor flaw.Series resistance in ourrecordings was generally low (2.5 – 4.5 MΩ) due to our use of patch pipettes with large openings. This should result in small voltage errors (<5 mV) when currents are in the low nA range, even without compensation. However, considering the often very high whole-cell current amplitudes in cells overexpressing TMEM206, there may indeed be a substantial voltage clamp error. We added a sentence in the Results section (subsection “Human TMEM206 mediates typical ASOR currents”, third paragraph) to caution the reader about this possibly confounding factor.

The authors point out in the Introduction that the name "PAORAC" provides a better description compared to "ASOR" and in addition, it is actually the older name. This, of course, is flattering to me as I coined the term PAORAC many years back. Maybe, the authors want to consider to use the term "PAORAC" also in the title, possibly together with the newer "ASOR".

To fairly acknowledge the earlier description of the current, we now added the PAORAC acronym (which in contrast to ASOR refers specifically to anion channels) to the title of our manuscript and to the Abstract.

In Figure 7E, it is hard to identify that the "three stars" of the L315D mutation belong to this panel, as they are plotted between panel E and F.

We changed the figure to clarify what the asterisks mean.

In Figure 7G and H, why is there no statistics reported?

We added statistics of the current densities with Na2SO4 as charge carrier in Figure 7H. However, we prefer not to perform statistical analysis on the data in Figure 7G as we believe this would mislead the reader into inferring meaningful differences between the mutants. Apparent differences are likely to be due to variable current amplitudes in Na2SO4 solutions with some mutants (see panel H), for which background currents (very negative reversal potential in our recording system) contribute more to the observed reversal potential. Also, we believe that a statistical comparison between measured reversal potentials and “not applicable” has little meaning. It is now clearly pointed out in the figure legend that no statistical analysis was performed for this panel to prevent misleading the reader.

In addition to the changes made to accommodate the suggestions of the reviewers, we also added a new figure supplement (Figure 3—figure supplement 1), which shows ICl,H measured in WT and TMEM206-/- HeLa cells. Two additional HeLa KO clones not described in the initial submission have been measured and are now incorporated in Table 1.


Article and author information

Author details

  1. Florian Ullrich

    1. Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin, Germany
    2. Max-Delbrück-Centrum für Molekulare Medizin (MDC), Berlin, Germany
    Conceptualization, Formal analysis, Validation, Investigation, Visualization, Writing—review and editing, Developed the genomic siRNA screening procedure, Optimized, performed and evaluated it together with KL, Performed all electrophysiology experiments
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-1153-2040
  2. Sandy Blin

    1. Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin, Germany
    2. Max-Delbrück-Centrum für Molekulare Medizin (MDC), Berlin, Germany
    Formal analysis, Validation, Investigation, Visualization, Writing—review and editing, Performed IHC, topology, and cell death experiments
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-5762-5149
  3. Katina Lazarow

    Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin, Germany
    Formal analysis, Validation, Investigation, Writing—review and editing, Together with FU evaluated the genomic siRNA screening procedure
    Competing interests
    No competing interests declared
  4. Tony Daubitz

    1. Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin, Germany
    2. Max-Delbrück-Centrum für Molekulare Medizin (MDC), Berlin, Germany
    Formal analysis, Validation, Investigation, Visualization, Writing—review and editing, Performed cell volume measurements
    Competing interests
    No competing interests declared
  5. Jens Peter von Kries

    Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin, Germany
    Resources, Supervision, Funding acquisition, Supervised siRNA screen
    Competing interests
    No competing interests declared
  6. Thomas J Jentsch

    1. Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin, Germany
    2. Max-Delbrück-Centrum für Molekulare Medizin (MDC), Berlin, Germany
    3. NeuroCure Cluster of Excellence, Charité Universitätsmedizin, Berlin, Germany
    Conceptualization, Resources, Formal analysis, Supervision, Funding acquisition, Validation, Writing—original draft, Writing—review and editing, Planned and evaluated experiments, Wrote the paper
    For correspondence
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-3509-2553


H2020 European Research Council (Advanced Grant VOLSIGNAL (#740537))

  • Thomas J Jentsch

EMBO (Long-Term Fellowship ALTF 665-2017)

  • Sandy Blin

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.


We thank Sabrina Kleissle for excellent help with the genome-wide siRNA screen, Marc Wippich for help with data analysis, and Judith von Sivers for technical assistance. This work was supported by the European Research Council (ERC) Advanced Grant VOLSIGNAL (#740537) to TJJ and an EMBO Long-Term Fellowship (ALTF 665-2017) to SB.

Senior Editor

  1. Olga Boudker, Weill Cornell Medicine, United States

Reviewing Editor

  1. Reinhard Jahn, Max Planck Institute for Biophysical Chemistry, Germany


  1. Reinhard Jahn, Max Planck Institute for Biophysical Chemistry, Germany
  2. Cecilia M Canessa, Tsinghua University, China
  3. Johannes Oberwinkler, Philipps-Universität Marburg, Germany

Publication history

  1. Received: June 10, 2019
  2. Accepted: July 17, 2019
  3. Accepted Manuscript published: July 18, 2019 (version 1)
  4. Version of Record published: July 29, 2019 (version 2)


© 2019, Ullrich et al.

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.


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