C-mannosylation supports folding and enhances stability of thrombospondin repeats
Abstract
Previous studies demonstrated importance of C-mannosylation for efficient protein secretion. To study its impact on protein folding and stability, we analyzed both C-mannosylated and non-C-mannosylated thrombospondin type 1 repeats (TSRs) of netrin receptor UNC-5. In absence of C-mannosylation, UNC-5 TSRs could only be obtained at low temperature and a significant proportion displayed incorrect intermolecular disulfide bridging, which was hardly observed when C-mannosylated. Glycosylated TSRs exhibited higher resistance to thermal and reductive denaturation processes, and the presence of C-mannoses promoted the oxidative folding of a reduced and denatured TSR in vitro. Molecular dynamics simulations supported the experimental studies and showed that C-mannoses can be involved in intramolecular hydrogen bonding and limit the flexibility of the TSR tryptophan-arginine ladder. We propose that in the endoplasmic reticulum folding process, C-mannoses orient the underlying tryptophan residues and facilitate the formation of the tryptophan-arginine ladder, thereby influencing the positioning of cysteines and disulfide bridging.
Introduction
Protein glycosylation is a major form of co- and post-translational modification, affecting the majority of secreted and cell-surface proteins. It can influence the folding of a protein as well as its physical properties, activity and ability to interact with other macromolecules, thus playing an important role in a great variety of cellular processes (Varki, 2017). C-mannosylation is presently one of the less well characterized glycosylation types. It takes place in the endoplasmic reticulum (ER), presumably co-translationally (Doucey et al., 1998; Krieg et al., 1998), and is performed by C-mannosyltransferase enzymes of the DPY(dumpy)-19 family (Buettner et al., 2013; Niwa et al., 2016; Shcherbakova et al., 2017). C-mannosylation has been found on tryptophans of WXXW/C motifs and involves a carbon-carbon linkage between the sugar and the protein: the C1 atom of the α-mannose is thereby attached to the indole C2 atom of the tryptophan (Figure 1A) (de Beer et al., 1995; Hofsteenge et al., 1994). However, the function of C-mannosylation remains elusive.
C-mannosylation has been predicted on 18% of human secreted and transmembrane proteins (Julenius, 2007). There are two main protein groups bearing conserved C-mannosylation sites – proteins with thrombospondin type 1 repeats (TSRs) and type I cytokine receptors. TSRs are small protein domains consisting of around 60 amino acids. They possess a conserved structure of three antiparallel strands typically linked by three disulfide bridges (Figure 1B) (Tan et al., 2002). The first strand contains the C-mannosylation motif with up to three tryptophan residues (WXXWXXWXXC), which all can be C-mannosylated (Hofsteenge et al., 1999). The tryptophans intercalate with conserved arginine residues from the second strand building a so-called tryptophan-arginine (Trp-Arg) ladder that forms the core of the TSR fold (Figure 1B) (Tan et al., 2002; Tossavainen et al., 2006). Due to stabilizing cation-π interactions between the arginine and tryptophan side chains (Gallivan and Dougherty, 1999), the Trp-Arg ladder is deduced to have an important structural function in the TSRs.
Several studies have shown that C-mannosylation is important for proper secretion of TSR-containing and other proteins. Mutagenesis of the C-mannosylation sites usually resulted in reduced secretion or cell-surface expression of the proteins and their retention in the ER (Fujiwara et al., 2016; Gouyer et al., 2018; Hilton et al., 1996; Niwa et al., 2016; Okamoto et al., 2017; Sasazawa et al., 2015; Taylor et al., 1997). The same effects were observed in cells with reduced availability of the donor substrate dolichol-P-mannose (Perez-Vilar et al., 2004; Wang et al., 2009), suggesting a direct influence of C-mannose on secretion efficiency; however, other glycosylation processes were affected in these cells as well. With the discovery of the enzyme catalyzing C-mannosylation – the C-mannosyltransferase (Buettner et al., 2013) – specific genetic intervention affecting C-mannosylation became possible, enabling to study the effects of C-mannosylation without affecting the target protein sequence or other cellular pathways. This indeed allowed to demonstrate that lack of C-mannosylation alone was responsible for reduced secretion of TSR-containing proteins (Buettner et al., 2013; Niwa et al., 2016; Shcherbakova et al., 2017). In this study, we utilized the possibility to produce a single TSR with and without C-mannoses in the same expression system to directly evaluate the effects of C-mannosylation beyond secretion.
Results
C-mannosylation becomes critical for secretion of UNC-5 TSRs with increasing temperature
Secretion of TSRs from C. elegans netrin receptor UNC-5 (Figure 1C) was analyzed at 20, 24°C and 28°C in naturally C-mannose-negative Drosophila S2 cells (Hofsteenge et al., 2001; Krieg et al., 1997). C-mannosylation of the TSRs was obtained by co-expression of the C. elegans DPY-19 C-mannosyltransferase. A C-mannosylation-independent Notch EGF16–20 fragment was used as transfection and secretion control.
At 20°C, secretion of UNC-5 TSRs was higher from cells co-expressing DPY-19 than from C-mannosylation-negative cells (Figure 2). At increasing temperatures, secretion of C-mannosylated TSRs was not affected, whereas secretion of non-mannosylated TSRs further declined gradually. Intracellular UNC-5 TSR levels were comparable at all conditions confirming that the cells were able to produce C- and non-mannosylated TSRs per se (Figure 2). Thus, impaired temperature-dependent secretion of non-mannosylated TSRs implied a role of C-mannoses in protein stability and/or protein folding.
C-mannosylation increases resistance of UNC-5 TSR2 to thermal denaturation
To explore the impact of C-mannosylation on TSR stability, the second TSR (TSR2) of UNC-5 (Figure 1B,C) was produced with and without C-mannoses in Drosophila S2 cells. Presence of C-mannoses was verified by mass spectrometry, showing that the major fraction of the protein was modified on the first two tryptophans of the WXXWXXW motif (Figure 3—figure supplement 1). The correct folding of both TSR forms was confirmed by circular dichroism (CD) spectroscopy showing TSR-typical spectra with characteristic maxima near 230 and 212 nm and a minimum in the 195–200 nm region (Figure 3A) (Huwiler et al., 2002; Roszmusz et al., 2002; Smith et al., 1984). Disappearance of the 230 nm maximum was used to monitor thermal unfolding of mannosylated and non-mannosylated TSRs (Figure 3B). The C-mannosylated TSR revealed a significantly higher melting temperature (Tm = 59.5°C) compared to the non-mannosylated form (Tm = 50.4°C) showing that C-mannosylation on W1 and W2 of the WXXWXXW motif strongly increases the resistance of the TSR to thermal denaturation.
Molecular dynamics simulation of the thermal denaturation
To understand the structural dynamics behind the increased resistance of the C-mannosylated TSR during thermal denaturation, we performed molecular dynamics simulations of a modeled UNC-5 TSR2 structure with and without C-mannosylation at elevated temperature of 75°C for 200 ns. Each simulation was repeated three times.
In both TSR forms, the Trp-Arg ladder scaffold was mostly maintained during the simulation, presumably because of restricted dynamics due to the three disulfide bridges. However, positions of arginines and tryptophans in the C-mannosylated TSR remained more constrained in all three simulation replicates (Figure 4A) indicating that the Trp-Arg ladder structure in the C-mannosylated TSR is more rigid compared to the non-modified TSR. Particularly Trp5 – the first tryptophan of the WXXWXXW motif – appeared highly flexible and diverged from the residual Trp-Arg ladder organization in the non-mannosylated form (Figure 4—videos 1 and 2). This was confirmed by calculated root-mean-square fluctuations (RMSF) of single residues as depicted by the blue-white-red color gradient of the representative TSR structures from each simulation replicate (Figure 4B, Figure 4—figure supplement 1). Correspondingly, calculated cation-π interactions between Trp5 and Arg24 were found to be maintained in the C-mannosylated TSR but not in the non-modified TSR (Figure 4—figure supplement 2).
Furthermore, we found hydrogen bond interactions between C-mannoses and the neighboring side chains as well as the backbone of the corresponding tryptophan residues throughout the simulations (Figure 4C,D and Figure 4—figure supplement 4). Thus, C-mannoses appeared to keep the tryptophans in specific orientations not only by affecting their conformational space but also by forming polar interactions with surrounding residues.
We performed the simulations with mannoses in a 4C1-chair conformation according to previously published crystal structures of C-mannosylated TSRs (Aleshin et al., 2012; Hamming et al., 2012). However, because NMR studies (de Beer et al., 1995; Nishikawa et al., 2005) and recently resolved TSR crystal structures (Pedersen et al., 2019; Pronker et al., 2016; van den Bos et al., 2019) suggest that C-mannoses can occur in 4C1 and 1C4 forms, we also performed a simulation with C-mannoses in 1C4-chair arrangement (Figure 4—video 3). For both conformations, we observed similar maintenance of the Trp-Arg ladder and ability to form hydrogen bonds (Figure 4—figure supplements 3 and 4).
C-mannosylation decreases the unfolding rate of UNC-5 TSR2 under reducing conditions
To evaluate the effects of C-mannosylation on the structure of a TSR aside from the impact exerted by the disulfide bonds, we analyzed non- and C-mannosylated UNC-5 TSR2 under reducing conditions by CD spectroscopy. Upon addition of 2 mM DTT, both proteins started to unfold immediately, showing that the disulfides are essential for the TSR structure. Denaturation of the C-mannosylated TSR, however, occurred approximately 1.8 times slower than that of the non-mannosylated repeat (Figure 5A). C-mannosylation might decrease the accessibility of the disulfide bridges by DTT, but could also protect the Trp-Arg ladder organization of the TSR.
To visualize putative structural effects of C-mannosylation, we performed molecular dynamics simulation studies of non- and C-mannosylated UNC-5 TSR2 lacking disulfide bonds at 24°C. As in the thermal denaturation simulation, non-mannosylated TSR revealed an increased flexibility of Trp5 in the Trp-Arg ladder organization in comparison to the C-mannosylated TSR (Figure 5B,C and Figure 5—figure supplement 1). Additionally, Cys26 (marked by *) involved in the upper disulfide bridge showed lower fluctuation values and thus decreased flexibility in the C-mannosylated TSR (Figure 5C, Figure 5—figure supplement 1) which might be relevant for the disulfide bond formation during protein folding.
C-mannosylation supports folding of UNC-5 TSR2
To further investigate the role of C-mannoses in TSR folding, oxidative folding experiments were performed with non- and di-mannosylated UNC-5 TSR2. After reduction and unfolding of the TSRs with DTT and guanidine hydrochloride, the refolding of the proteins was monitored by CD spectroscopy in the presence of 1 mM reduced and 0.5 mM oxidized glutathione (GSH and GSSG) for three hours. Both TSRs were able to form native-like conformations as demonstrated by the appearance of the characteristic TSR peak in CD spectra (Figure 6A). Still, folding initiation of the C-mannosylated TSR occured significantly faster compared to the non-modified protein (Figure 6A, Figure 6—figure supplement 1), confirming that C-mannosylation plays a role in the folding of UNC-5 TSR2.
Indeed, purification of non- and C-mannosylated TSRs from Drosophila S2 cells showed that the folding of non-mannosylated TSRs was affected. Next to monomeric TSRs, a fraction with higher nickel affinity was secreted (Figure 6B). This fraction was interpreted as a covalently linked dimer since it showed a higher mobility in gel filtration (Figure 6B) and the double mass of the monomer in the MALDI-TOF spectrum (Figure 6—figure supplement 2). On SDS-PAGE, the mobility became identical to the monomer upon reduction by DTT (Figure 6B) indicating connection by aberrant disulfides. In cells co-expressing DPY-19 C-mannosyltransferase, dimeric TSR formation was significantly reduced. These results argue for an accelerated folding initiation of the C-mannosylated TSR in the ER and a critical role of C-mannosylated tryptophans for the native disulfide connections.
Discussion
C-mannosylation is a unique protein glycosylation type with a still unclear function. In the present study, we compared non- and C-mannosylated UNC-5 TSRs and were able to show that C-mannoses added on the first two tryptophans of the WXXWXXW motif affect the dynamics of a TSR and play an important role in its folding, stability and secretion.
Assuming that C-mannosylation is co-translational (Krieg et al., 1998) implies that it precedes and thereby assists correct Cys pairing to form the native disulfide bridges. Indeed, we observed increased formation of covalent dimers (Figure 6B) for TSR2 of C. elegans UNC-5 in the absence of C-mannosylation, which were caused by incorrect disulfide bond connections. Our molecular dynamics simulations of UNC-5 TSR2 lacking disulfide bridges showed that the Trp-Arg ladder was maintained when C-mannosylation was present (Figure 5B). Accordingly, we observed slower unfolding of the C-mannosylated TSR during protein denaturation under reductive conditions (Figure 5A). The simulation also showed that Cys26 fluctuated less if the tryptophans were C-mannosylated. In the process of protein folding, cysteines have to come in close vicinity to form bridges. The Trp-Arg ladder most likely plays an essential role in the correct positioning of cysteines. It is conceivable that the mannoses assist in the formation of the ladder and stabilize it before disulfide bridges are made. Our oxidative folding experiments indeed showed that the refolding initiation (appearance of the Trp-Arg ladder) was accelerated by the presence of C-mannoses (Figure 6A). This is also consistent with the view that protein domain folding precedes disulfide bridge formation (Robinson et al., 2017).
Once the TSR is folded, it is further stabilized by the three disulfide bridges, and although the shift of the maximum in the CD spectrum suggests that the tryptophan and arginine residues are in slightly different environments, the overall CD profiles are comparable in both TSR forms (Figure 3A). Nevertheless, C-mannosylated TSR was more resistant to thermal denaturation than non-mannosylated TSR (Figure 3B) revealing the importance of C-mannosylation also after folding of the TSR. Molecular dynamics simulations at elevated temperature showed that C-mannoses were able to built hydrogen bonds to the surrounding residues (Figure 4C,D) and kept especially the tryptophan closest to the N-terminus in place. Without C-mannose, this tryptophan was consistently losing its proper orientation in the ladder in repeated simulations, resulting in higher flexibility of the upper TSR region (Figure 4A,B). Existence of hydrogen bonds between C-mannoses and surrounding amino acids was also suggested in recent structural studies on properdin (Pedersen et al., 2019; Pronker et al., 2016; van den Bos et al., 2019).
The area around the third tryptophan was less affected. Since the C. elegans C-mannosyltransferase DPY-19 is not targeting this tryptophan, it remains unmodified in C. elegans UNC-5. In mammals, the third tryptophan of the WXXWXXWXXC sequence can be mannosylated by the DPY19L3 homolog (Hofsteenge et al., 1999; Shcherbakova et al., 2017). The requirement for C-mannosylation at this position, as well as on the first two tryptophans, might thus vary in different organisms and different proteins. Secretion of human R-spondin1 was highly reduced in DPY19L3-restricted cells (Niwa et al., 2016), whereas mouse UNC5A was not affected (Shcherbakova et al., 2017). Moreover, many TSRs have less than three tryptophans, and some might not be C-mannosylated at all (Verbij et al., 2016). In C. elegans MIG-21, the first TSR lacks two of the six cysteines and might, therefore, require C-mannosylation since it cannot be secreted without C-mannoses even at low temperatures (Buettner et al., 2013). On the other hand, multiple TSRs have been produced in native conformations in bacteria or insect cells without glycosylation (Klenotic et al., 2011; Tan et al., 2002; Tossavainen et al., 2006), showing that C-mannosylation is probably not an absolute requirement for some TSRs, but a critical factor for others.
C. elegans dpy-19 mutants deficient in C-mannosylation develop a temperature-sensitive dumpy phenotype (Honigberg and Kenyon, 2000) that we could reproduce (Figure 2—figure supplement 1). We assume that in these mutants, secretion and stability of various TSR-containing and possibly other proteins are affected. Since impairment of UNC-5 alone is not sufficient for the development of the dumpy phenotype (Hedgecock et al., 1990), further C-mannosylation-dependent proteins must be involved.
In conclusion, our experiments support a model in which C-mannosylation of tryptophans in the Trp-Arg ladder of TSRs can provide assistance in the folding process, but also greatly enhance the stability of the folded protein.
Materials and methods
Plasmids and protein constructs
Request a detailed protocolThe pIB vector (Invitrogen) was used for constitutive expression of the C. elegans DPY-19 protein (pIB-DPY-19) (Buettner et al., 2013). The pMT/BiP/V5-His A vector (Invitrogen) was used for expression of V5- and His-tagged C. elegans UNC-5 TSRs 1+2 and Drosophila Notch EGF repeats as well as His-tagged C. elegans UNC-5 TSR2 (corresponding protein sequences are depicted in Supplementary file 1).
Protein expression in S2 cells for western blot analysis
Request a detailed protocolTransfection of Drosophila S2 cells in Insect-XPRESS Protein-free Medium (Lonza) was performed using FuGENE HD (Promega) and Opti-MEM (Invitrogen) – 2 ml cells were transfected using 2 µg pMT-UNC-5-TSR1+2 plasmid and 1 µg pMT-Notch-EGF16–20. After 6 hr at 24°C, pMT-vector expression was induced with 0.2 mM CuSO4. Induced cells were sub-divided and incubated at 20, 24°C and 28°C for 3 days. 1/10th of the cell media was mixed with 5 × Laemmli (300 mM Tris-HCl [pH 6.8], 10% SDS, 50% glycerol, 25% β-mercaptoethanol, 0.05% bromophenol blue). 1/10th of cells was resuspended in 20 µl 1 × Laemmli. All samples were heated for 10 min at 95°C and separated by SDS-PAGE (5%/15%). Proteins were detected on a nitrocellulose membrane with mouse anti-V5 antibody (1:5000; Acris) and IRDye 800 conjugated goat anti-mouse secondary antibody (1:20000; LI-COR). Blots were scanned on a LI-COR Odyssey Infrared Scanner. Prestained marker bands (Page-Ruler, Fermentas) were detected at 700 nm. The experiment was repeated five times with independently transfected cells.
Protein expression in S2 cells for CD spectroscopy
Request a detailed protocol20 ml S2 cells were transfected either with 20 µg pMT-UNC-5-TSR2 plasmid or with 10 µg pMT-UNC-5-TSR2 and 10 µg pIB-DPY-19 as described above. Upon induction, cells expressing UNC-5 TSR2 were incubated at 24°C, and cells expressing UNC-5 TSR2 and DPY-19 at 28°C (to avoid secretion of non-mannosylated UNC-5 TSR2). After 4 days of incubation, cell media were centrifuged for 3 min at 300 × g and 4.500 × g, filtered through 0.2 µm cellulose acetate membrane (Waters) and dialyzed against 500 ml 20 mM Tris-HCl pH 8, 500 mM NaCl using 2000 MWCO dialysis tubes (Carl Roth). For nickel affinity chromatography, samples were mixed with 20 mM imidazole and loaded on 1 ml HisTrap HP columns (GE Healthcare). Proteins were eluted with a linear gradient of 20–350 mM imidazole over 7 ml and detected at 280 nm. Fractions containing monomeric UNC-5 TSR2 were applied to a 30 ml gel filtration column (Bio-Gel P-10 fine, Bio-Rad) in 10 mM KPi pH 7.6, 5 mM NaCl. Protein-containing fractions were concentrated using Vivaspin 6 MWCO 3000 (GE Healthcare).
Protein expression in S2 cells for oxidative folding
Request a detailed protocol200 ml of stably transfected S2 cells expressing either pMT-UNC-5-TSR2 alone or in combination with pIB-DPY-19 at a density of 3 × 106 cells/ml were induced using 4 µM CdCl2 and incubated for 4 days at 24 or 28°C, respectively. The cell media were centrifuged, filtered, dialyzed and purified by nickel affinity chromatography as described above. The monomeric proteins were additionally purified by C18 chromatography (Xbridge TM Prep C18 5 µm, 10 × 50 mm column, Waters) to separate di-, mono- and non-mannosylated TSR2 forms (Figure 6—figure supplement 3) prior to gel filtration in 100 mM Tris pH 8 and concentration.
CD spectroscopy, thermal and reductive denaturation
Request a detailed protocolMeasurements were performed with a 3 mm high precision quartz cuvette (Hellma Analytics) using protein solutions of 0.1 mg/ml (in 10 mM KPi pH 7.6, 5 mM NaCl) in a PiStar-180 (Applied Photophysics) system with the Equilibrium Sampling Handling Unit (ESHU). To obtain native CD spectra, scans with 5 nm bandwidth of 260–178 nm with 1 nm steps and 16 s/step sampling time were performed at 24°C. Three spectra were averaged and smoothed using the Savitsky-Golay algorithm (3-point smooth). For thermal denaturation, 24–95°C temperature ramping with 1°C steps and 1.5 °C/min ramping rate was used. For reductive denaturation, 2 mM DTT was added to the samples, and after 2 min mixing time, the kinetics were detected with 16 s/step sampling time for 3600 s at 24°C. In both denaturation experiments, the ellipticity changes were monitored at 228 nm for the C-mannosylated and at 229 nm for non-mannosylated TSR. To determine the melting temperatures, the average values from measurements of three protein samples, independently produced by transient transfection of S2 cells, were calculated and fitted by a Boltzmann function (OriginLab). For calculations of the half-time values, a first-order exponential decay function was used for the fitting (ExpDec1, OriginLab).
Molecular dynamics simulations
Request a detailed protocolA structural model of UNC-5 TSR2 was based on the available crystal structure of the human UNC5A TSR (PDB 4V2A, 62% amino acid positives and no gaps) using MODELLER (Sali and Blundell, 1993). Positions of C-mannoses on Trp5 and Trp8 were derived from the positions of C-mannoses in complement component C6 (3T5O), IL-21 receptor (3TGX) and ADAMTS13 (3VN4). Force field parameters for C-mannoses were created manually. Prior to MD simulations, the structural models were energy minimized using Schrödinger MacroModel and the OPLS3 force field (Schrodinger Suite, available online; Watts et al., 2014), and fully solvated with the TIP3P water model (Jorgensen et al., 1983). The net charge of the systems was neutralized by adding counter ions. All simulations were performed using NAMD 2.11 (Phillips et al., 2005) and the CHARMM27 force field (MacKerell et al., 1998). A time-step of 1 fs was used. Long-range electrostatics were treated with the particle mesh Ewald method (Darden et al., 1993) and a 12 Å cutoff was used for nonbonded short-range interactions. First, an additional energy minimization and a 5 ns equilibration were performed at a constant temperature of 310 K and pressure (1 atm), followed by 200 ns MD simulations. For the simulation at elevated temperature, mimicking thermal denaturation conditions, MD simulations were carried out at constant temperatures of 348 K for 200 ns. For the simulation of the reductive denaturation, all disulfide bonds between cysteine residues were removed. The simulations were performed for 200 ns at 297 K. For both conditions, three independent simulations were performed and compared.
Hydrogen bonds between tryptophan residues and the protein were determined at distances below 3.5 Å and the angle cutoff of 30°. RMSF values of side chain atoms were calculated using an RMSF script, averaged for each residue and used in PyMOL for the coloring of the protein structures (data2bfactor script, color_b script, The PyMOL Molecular Graphics System, Version 1.2r3pre, Schrödinger, LLC). Clusters of most frequent conformations of the Trp-Arg ladder were determined by hierarchical clustering (Kelley et al., 1996) of the simulation trajectories at elevated temperatures. The calculated representative structures from the largest cluster of each simulation were used for RMSF-coloring. For the simulation of reducing conditions, the last structure of each simulation (after 200 ns) was used to demonstrate the extent of denaturation in non- and C-mannosylated TSRs.
For the calculation of putative cation-π interactions in the protein structures, PDB files of single frames from the MD trajectories were analyzed with the CaPTURE program (Gallivan and Dougherty, 1999).
Oxidative folding
Request a detailed protocolNon- and C-mannosylated UNC-5 TSR2 (0.14 mg/ml) were denatured in 200 mM DTT and 6 M guanidine hydrochloride overnight at 24°C. The samples were desalted at 32°C using a 5 ml HiTrap desalting column (GE Healthcare) and the folding buffer (50 mM Tris HCl pH 8.4, 100 mM NaCl). The refolding reaction was performed in the presence of 0.5 mM GSSG and 1 mM GSH (Sigma) at 24°C and was monitored for 3 hr by CD spectroscopy. Scans of 260–190 nm were performed with 1 nm steps and 8 s/step sampling time, whereby 10 min per scan were required. The experiment was performed four times in total, at two temperatures (24°C and 32°C), whereby the same effect was observed.
Data availability
All data generated or analysed during this study are included in the manuscript and supporting files. Source data files have been provided for Figures 3, 4 and 5.
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Article and author information
Author details
Funding
Deutsche Forschungsgemeinschaft (FOR2509 BA 4091/6-1)
- Hans Bakker
Deutsche Forschungsgemeinschaft (BA 4091/5-1)
- Hans Bakker
Deutsche Forschungsgemeinschaft (BU 2920/2-1)
- Falk FR Buettner
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2019, Shcherbakova et al.
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
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- Biochemistry and Chemical Biology
- Structural Biology and Molecular Biophysics
Dynamic conformational and structural changes in proteins and protein complexes play a central and ubiquitous role in the regulation of protein function, yet it is very challenging to study these changes, especially for large protein complexes, under physiological conditions. Here, we introduce a novel isobaric crosslinker, Qlinker, for studying conformational and structural changes in proteins and protein complexes using quantitative crosslinking mass spectrometry. Qlinkers are small and simple, amine-reactive molecules with an optimal extended distance of ~10 Å, which use MS2 reporter ions for relative quantification of Qlinker-modified peptides derived from different samples. We synthesized the 2-plex Q2linker and showed that the Q2linker can provide quantitative crosslinking data that pinpoints key conformational and structural changes in biosensors, binary and ternary complexes composed of the general transcription factors TBP, TFIIA, and TFIIB, and RNA polymerase II complexes.
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- Biochemistry and Chemical Biology
- Stem Cells and Regenerative Medicine
Human induced pluripotent stem cells (hiPSCs) have great potential to be used as alternatives to embryonic stem cells (hESCs) in regenerative medicine and disease modelling. In this study, we characterise the proteomes of multiple hiPSC and hESC lines derived from independent donors and find that while they express a near-identical set of proteins, they show consistent quantitative differences in the abundance of a subset of proteins. hiPSCs have increased total protein content, while maintaining a comparable cell cycle profile to hESCs, with increased abundance of cytoplasmic and mitochondrial proteins required to sustain high growth rates, including nutrient transporters and metabolic proteins. Prominent changes detected in proteins involved in mitochondrial metabolism correlated with enhanced mitochondrial potential, shown using high-resolution respirometry. hiPSCs also produced higher levels of secreted proteins, including growth factors and proteins involved in the inhibition of the immune system. The data indicate that reprogramming of fibroblasts to hiPSCs produces important differences in cytoplasmic and mitochondrial proteins compared to hESCs, with consequences affecting growth and metabolism. This study improves our understanding of the molecular differences between hiPSCs and hESCs, with implications for potential risks and benefits for their use in future disease modelling and therapeutic applications.