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Phasic oxygen dynamics confounds fast choline-sensitive biosensor signals in the brain of behaving rodents

  1. Ricardo M Santos  Is a corresponding author
  2. Anton Sirota  Is a corresponding author
  1. Bernstein Center for Computational Neuroscience, Faculty of Medicine, Ludwig-Maximilians Universität München, Germany
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Cite this article as: eLife 2021;10:e61940 doi: 10.7554/eLife.61940

Abstract

Cholinergic fast time-scale modulation of cortical physiology is critical for cognition, but direct local measurement of neuromodulators in vivo is challenging. Choline oxidase (ChOx)-based electrochemical biosensors have been used to capture fast cholinergic signals in behaving animals. However, these transients might be biased by local field potential and O2-evoked enzymatic responses. Using a novel Tetrode-based Amperometric ChOx (TACO) sensor, we performed highly sensitive and selective simultaneous measurement of ChOx activity (COA) and O2. In vitro and in vivo experiments, supported by mathematical modeling, revealed that non-steady-state enzyme responses to O2 give rise to phasic COA dynamics. This mechanism accounts for most of COA transients in the hippocampus, including those following locomotion bouts and sharp-wave/ripples. Our results suggest that it is unfeasible to probe phasic cholinergic signals under most behavioral paradigms with current ChOx biosensors. This confound is generalizable to any oxidase-based biosensor, entailing rigorous controls and new biosensor designs.

Introduction

Acetylcholine (ACh) is an essential modulator of neuronal circuits engaged in high order cognitive operations. During aroused states, high extracellular ACh sets cortico-hippocampal circuits toward memory encoding by enhancing sensory processing, synaptic plasticity and neuronal network rhythmicity (Hasselmo and McGaughy, 2004; Marrosu et al., 1995; Steriade, 2004; Teles-Grilo Ruivo and Mellor, 2013). The latter is particularly relevant in the hippocampus, where ACh plays an important role in the processing of episodic and emotional information via modulation of theta oscillations (Buzsáki, 2002; Gu et al., 2017; Li et al., 2007; Mikulovic et al., 2018; Vandecasteele et al., 2014). Contrarily, low tonic ACh during non-REM sleep permits the occurrence of hippocampal sharp-wave/ripple complexes (SWRs), which are critical for memory consolidation (Buzsáki, 2015; Hasselmo and McGaughy, 2004; Norimoto et al., 2012; Vandecasteele et al., 2014).

The current theory on the functional role of ACh in cortical and hippocampal circuits has been mainly derived from brain-state-related correlations of tonic ACh levels with underlying network dynamics, or from strong manipulations of the cholinergic system (Gu et al., 2017; Hasselmo and McGaughy, 2004; Li et al., 2007; Marrosu et al., 1995; Norimoto et al., 2012; Vandecasteele et al., 2014). However, such crude analytic and experimental approaches cannot account for the spontaneous non-stationary interactions between ACh, behavior and neuronal network activity. Recently, fine time-scale measurements of cholinergic activity have provided new insights into this interplay. Fast cholinergic transients in cortical and hippocampal regions have been described in response to sensory sampling, unexpected events, negative reinforcements, and reward-related behavior (Eggermann et al., 2014; Hangya et al., 2015; Howe et al., 2017; Lovett-Barron et al., 2014; Parikh et al., 2007; Teles-Grilo Ruivo et al., 2017). The latter have been captured using electrochemical biosensors in response to detection of cues to rewards and reward approach or retrieval in freely moving rodents (Howe et al., 2017; Parikh et al., 2007; Teles-Grilo Ruivo et al., 2017). The temporally precise alignment of phasic ACh signals to these events hints for a critical role of ACh on the formation of reward-related memories and on the guiding of learned reward-oriented actions.

The above-mentioned studies highlight the suitability of enzyme-based electrochemical biosensors to capture phasic release of neurotransmitters and neuromodulators (Chatard et al., 2018). Additionally, amperometric measurements pick-up currents generated by the local field potential (LFP) (Santos et al., 2015; Viggiano et al., 2012; Zhang et al., 2009), making these sensors ideal for studying the interplay between neuromodulatory tone and neuronal network dynamics. The most successful electrochemical ACh-sensing strategy in vivo has relied on the Choline Oxidase (ChOx)-mediated measurement of extracellular choline (Ch), a product of ACh hydrolysis by acetylcholinesterase. The enzyme catalyzes Ch oxidation in the presence of O2, generating H2O2, which is oxidized on the electrode surface (Burmeister et al., 2003; Parikh et al., 2004; Parikh et al., 2007; Teles-Grilo Ruivo et al., 2017; Zhang et al., 2010).

However, despite the apparent success of ChOx-biosensors, the factors that can confound their response in vivo at the fast time-scale, such as LFP-related artifacts and O2-evoked enzyme transients, have not been thoroughly addressed.

Chemical modification of the electrode surface has been a common approach used to effectively reduce electrodes’ response to electroactive substances (e.g. ascorbate or dopamine) (Burmeister et al., 2003; Zhang et al., 2010; Baker et al., 2015). Cancellation of neurochemical artifacts generating faradaic currents has been further improved in multi-site sensor designs (Burmeister et al., 2003; Santos et al., 2015; Zhang et al., 2010). By differentially coating the recording sites with matrices that contain or lack the enzyme, sites can be rendered Ch-sensitive or not (sentinel sites), enabling differential measurements with improved selectivity. Although the combination of these two strategies has proven effective on neurochemical artifact removal, the differential coating of the recording sites is not optimal to remove capacitive currents arising from fluctuations in the local field potential (Zhang et al., 2009). Although differential measurements are essential to clean biosensor signals (Santos et al., 2015; Zhang et al., 2010), cross-talk caused by H2O2 diffusion from enzyme-coated to sentinel sites poses important constraints on the sensor design. The inter-site spacing required to avoid diffusional cross-talk (typically >150 μm, depending on enzyme loadings) leads to uncontrolled differences in the amplitude and phase of LFP across sites, compromising common-mode rejection.

Furthermore, the strategies devised to reduce artifacts that directly generate electrochemical currents (chemical surface modifications or common-mode rejection) and are unrelated to enzymatic response to Ch, do not control for factors influencing immobilized ChOx activity (COA). Given that O2 is a co-substrate of the enzyme, it is crucial to control whether physiological O2 variations can contribute to biosensor responses in vivo leading to distortion of true and detection of false-positive Ch signals. Previous studies have only shown that O2 steady-state responses of ChOx-based biosensors in vitro follow apparent Michaelis-Menten saturation kinetics (Baker et al., 2017; Burmeister et al., 2003; Santos et al., 2015). The relatively narrow linear range of O2-dependent biosensor responses, as compared with estimates of average O2 levels in the brain, has motivated the assumption that ChOx-based biosensors are not affected by in vivo O2 dynamics (Baker et al., 2017; Burmeister et al., 2003). That might, however, oversimplify the effect of O2 on enzymatic activity since its basal levels and activity-related phasic dynamics widely vary across brain regions and experimental conditions (Lyons et al., 2016; Murr et al., 1994; Nair et al., 1987). Furthermore, previous literature has ignored possible non-steady-state (phasic) biosensor responses to O2, which might arise from local consumption and diffusion of enzyme substrates and reaction products in the sensor coating. These putative transient sensor responses to O2 are particularly relevant as they might temporally overlap with fast cholinergic transients. Therefore, the full assessment of biosensors’ O2 dependence requires the characterization of tonic and phasic O2-evoked responses and the simultaneous in vivo measurement of COA (biosensor response) and O2 within the sensor substrate. Yet, when addressed, O2 levels in the tissue have been measured using a separate electrode, often of different geometry and/or having a surface material or modification that differs from the Choline-sensing site (Baker et al., 2015; Dixon et al., 2002; Santos et al., 2015). This approach is therefore prone to bias from heterogeneous tissue O2 dynamics and differential kinetics of electrode responses to O2. Importantly, these studies have only characterized effect of very slow, tonic changes in O2 levels on sensor response.

Here, we have implemented a novel sensing approach based on differential modification of recording sites’ electrocatalytic properties toward H2O2, resulting in Ch-sensitive and pseudo-sentinel sites. As Ch (or COA) responses depended solely on the intrinsic properties of the metal surface, we could dramatically reduce the size and increase the spatial density of recording sites by using tetrodes as the electrode support. The Tetrode-based Amperometric ChOx (TACO) sensor provides differential responses to changes in COA, interferents and LFP across four bundled 17 μm diameter Pt/Ir wires. Importantly, this multichannel configuration allows highly sensitive measurement of COA and O2 in the same brain spot by using a tetrode site to directly measure the latter. This has not been possible to achieve with conventional enzyme-based biosensors, whose design was constrained by diffusional cross-talk.

We show that the TACO sensor provides a highly selective and sensitive measurement of COA when recording from the brain of behaving animals, effectively suppressing artifacts caused by neurochemicals, LFP and movement. But remarkably, a detailed in vitro characterization and mathematical modeling of biosensors revealed a novel phasic component of sensor’s O2 dependence caused by non-stationary enzyme responses to phasic O2 changes. Accordingly, measurements with the TACO sensor in behaving animals revealed fast temporally- and amplitude-correlated O2 and COA dynamics following locomotion bouts and hippocampal SWRs. Causal analysis of this correlation via local or systemic manipulation of O2 dynamics in vivo demonstrated that O2 transients can cause phasic COA responses. Our results demonstrate that the biosensor’s phasic O2 dependence causes transient biosensor signals in response to physiological fluctuations in O2. The extent and complexity of O2-related confounds is such that extraction of authentic cholinergic dynamics from the signal is not feasible with currently methodology. Importantly, this O2 ChOx-confounding dynamics is associated with behaviorally and physiologically relevant events and warrants important implications for the interpretation of previous studies relying on ChOx and other oxidase-based sensors as well as for the design of future enzyme-based sensors.

Results

The TACO sensor provides a highly selective differential measurement of COA

The TACO sensor is built around Pt/Ir wire tetrode, providing four disc-shaped recording sites with 17 μm diameter in close proximity, resulting in an entire sensor diameter of approximately 60 μm (Figure 1A). Such spatial density of recording sites is ideal for common-mode rejection of LFP-related currents and neurochemical dynamics. At this spatial scale, diffusional crosstalk would preclude the use of sentinel sites by differential coatings, as done in conventional biosensors, including our previous ChOx biosensor design (Burmeister et al., 2003; Santos et al., 2015). Instead, we created pseudo-sentinel and Ch-sensing sites by differentially plating the tetrode wires, modifying their electrocatalytic response toward H2O2. This step was followed by coating the tetrode surface with a common matrix containing ChOx entrapped in chitosan (Santos et al., 2015; Figure 1A). As for the initial plating steps, all tetrode sites were first mildly plated with gold, which marginally increased the electrode surface area, as inferred from impedances at 1 kHz (419 ± 33 kΩ, n = 28 before vs. 370 ± 16 kΩ, n = 44 after gold plating). Despite the slight decrease in impedance, gold-plating significantly decreased the electrocatalysis of H2O2 reduction/oxidation at the metal surface. In enzyme-coated electrodes, gold-plated sites exhibited a nearly fivefold smaller H2O2 sensitivity than an unplated Pt/Ir surface (Figure 1—figure supplement 1). Remarkably, following this first step, gold-plated sites could be rendered H2O2-sensitive upon mild platinization. In enzyme-coated electrodes, there was a nearly 10-fold difference in H2O2 oxidation currents between Au and Au/Pt sites at 0.4–0.6 V vs. Ag/AgCl (Figure 1B). The increase in Au sites’ response to H2O2 above +0.7 V is in agreement with the electrochemical behavior of a pure Au electrode and probably results from the formation of surface oxides (Burke and Nugent, 1997; O'Neill et al., 2004). Interestingly, the response of Au/Pt electrodes to H2O2 was higher than that of unplated electrodes (Figure 1—figure supplement 1), possibly reflecting an increase in the electrode surface area and/or an electrocatalytic effect caused by the deposition of nanostructured platinum over the gold surface (Burke and Buckley, 1996; Domínguez-Domínguez et al., 2008). Even when using common-mode rejection, decreasing the magnitude of interferences in individual sites is desirable. Thus, after plating and coating the tetrode, we electropolymerized m-PD in two of the recording sites, in order to reduce responses to electroactive compounds larger than H2O2 (e.g. ascorbate or dopamine) (Hascup et al., 2013; Santos et al., 2008). The final TACO sensor configuration consisted of all possible combinations of Pt and m-PD modifications of Au-plated recording sites (Figure 1A).

Figure 1 with 1 supplement see all
TACO sensor design and response properties.

(A) Schematics depicting the multichannel biosensor design, including the assembly used in in vitro and head-fixed recordings (bottom). HS: head-stage. (B) Voltammogram showing H2O2 sensitivities of gold-plated and platinized sites upon amperometric calibrations at different DC potentials (n = 10). Prior to calibrations, tetrodes were coated with a matrix of chitosan/ChOx. (C) Top shows a representative calibration of a sensor showing the response of different types of sites to step additions of Ch, H2O2, dopamine (DA). and ascorbate (AA). Bottom shows an example of current responses to a sinusoidal 12 mV AC voltage at 0.2 Hz overlaid on top of +0.6 V vs. Ag/AgCl DC voltage. (D) Sensitivities of different sites toward Ch (n = 10 biosensors). Unlike m-PD electropolymerization, platinization significantly increased sensitivity (p<0.0001 and F1,33 = 115 for platinization effect and p=0.87 and F1,33 = 0.03 for m-PD, by two-way ANOVA for unbalanced data). (E) Normalized responses of each tetrode site to Ch, interferent molecules and AC voltage, presented as impedance at 0.2 Hz (n = 5–10). Magnitudes significantly depended on the site modification and on the factor tested (p<0.0001 and F1,113 = 22 for platinization, F1,113 = 109 for m-PD and F3,113 = 12.9 for factors, by three-way ANOVA for unbalanced data). Platinization selectively increased responses to Ch (p<0.0001) while m-PD decreased responses only to DA and AA (p<0.0001). Impedances did not significantly differ across different types of electrode modifications (p>0.99). The rightmost column shows COA signal responses computed from the difference between Au/Pt/m-PD and Au/m-PD sites. Groups were compared by three-way ANOVA followed by Tukey-Kramer post-hoc tests. Data are represented as mean ± CI.

The responses of TACO sensors’ sites to Ch, H2O2 and to compounds that can potentially interfere during in vivo measurements were tested by step additions in the beaker at +0.6V vs. Ag/AgCl (Figure 1C, top). In accordance with the voltammograms of H2O2 sensitivities (Figure 1B), the responses of TACO sensors’ gold-plated sites to Ch and H2O2 were much lower than those of platinized sites. On average, like for H2O2, this difference was about 10-fold for Ch, regardless of m-PD electropolymerization (Figure 1D). Contrasting with its lack of effect on Ch sensitivity, m-PD dramatically decreased responses to ascorbate and dopamine, regardless of the site’s metal composition (Figure 1E). In addition, we have also calibrated the impedance of recording sites at low frequency by applying a low-amplitude 0.2 Hz AC voltage on top of the DC offset. This low frequency is of particular relevance, as it overlaps with putative phasic cholinergic dynamics previously reported by us and other groups in anesthetized and freely moving rodents (Howe et al., 2017; Parikh et al., 2007; Santos et al., 2015; Teles-Grilo Ruivo et al., 2017). Impedances calculated from the current oscillations generated by the AC voltage (Figure 1C, bottom) were comparable across all sites (Figure 1E). Collectively, the results summarized in Figure 1E and Table 1 validate the gold-plating approach to produce pseudo-sentinel sites, as it selectively reduces electrode’s response to H2O2. The COA signal most consistently used throughout this study was computed from the differential of m-PD-electropolymerized sites, to exploit the advantages of both differential platings and m-PD electropolymerization, resulting in a high selectivity for Ch (or changes in COA) (Figure 1E and Table 1). As compared to our previous stereotrode design using 50 μm diameter wires, these sensors keep the same Ch response performance, with a limit of detection (LOD) in the low nanomolar range, remarkable for such small electrode surfaces. The TACO sensor response is stable, without significant drop upon in vivo head-fixed recordings (2.45 ± 1.11 pA/μM before and 2.46 ± 0.80 pA/μM after implantation, n = 8, p=0.99, paired t-test), shows high linearity within the physiological range and a T50 response time around 1.5 s (Table 1). Noteworthy, though the response of our sensors is expected to be mostly shaped by Ch diffusion in the coating (Santos et al., 2015), the presented response times are in fact slightly overestimated due to a delay caused by mixing of the analyte in the stirred calibration buffer. Additionally, while m-PD abrogates the response of electropolymerized sites to large electroactive molecules, the differential site modifications provide further information on the signal identity. As the Au site (without m-PD) is more responsive to interferents than to Ch, the neurochemical confounds (NCC) signal (please see Materials and methods section) enables to further infer contaminations by neurochemical confounds in vivo. The differential sites’ responses to different factors can potentially be further exploited by multivariate methods of analysis, and the electrochemical tetrode design employed in TACO sensor can be generalized to other types of sensors in the future.

Table 1
Analytical properties of TACO sensors.
Individual sites’ analytical properties
Channel typeCh sensitivity (pA/µM)Ch sensitivity (nA µM−1 cm−2)H2O2 sensitivity (pA/µM)DA sensitivity (pA/µM)AA sensitivity (pA/µM)Impedance (GΩ)
Au/Pt/m-PD2.45 ± 0.70
(n = 10)
1081 ± 309
(n = 10)
2.48 ± 0.93
(n = 8)
0.15 ± 0.23(n = 10)0.02 ± 0.013(n = 10)2.90 ± 0.48
(n = 5)
Au/Pt2.54 ± 0.64
(n = 8)
1118 ± 283
(n = 8)
2.86 ± 1.10
(n = 6)
7.48 ± 1.86(n = 8)0.22 ± 0.20(n = 8)3.38 ± 0.56
(n = 5)
Au/m-PD0.27 ± 0.12
(n = 10)
118.2 ± 53
(n = 10)
0.22 ± 0.16
(n = 8)
0.13 ± 0.23
(n = 10)
0.007 ± 0.004 (n = 10)3.11 ± 0.77
(n = 5)
Au0.25 ± 0.086
(n = 9)
111.5 ± 38
(n = 10)
0.29 ± 0.08
(n = 7)
8.24 ± 1.84
(n = 9)
0.19 ± 0.16
(n = 9)
3.65 ± 0.59
(n = 5)
Analytical properties for COA measurement (Au/Pt/m-PD - Au/m-PD)
Ch sensitivity (pA/µM)Limit of detection (nM)Linearity,
[Ch]<30 µM (R2)
Response time (s)DA sensitivity (pA/µM), selectivity ratioAA sensitivity (pA/µM), selectivity ratio
2.18 ± 0.73
(n = 10)
28 ± 0.011
(n = 10)
0.9996 ± 0.0003
(n = 10)
1.4 ± 0.4
(n = 10)
0.022 ± 0.38
(n = 10),
101:1
0.013 ± 0.012
(n = 10),
169:1
  1. The data are given as the mean ± CI (95%).

    The number of sensors tested is given in parentheses. Data were collected from calibrations on the day after m-PD electropolymerization.

Freely moving recordings validate the TACO sensor suppression of current-generating artifacts and suggest a potential O2 modulation of COA in vivo

In order to test whether the TACO sensor can measure fast COA transients devoid of artifacts that directly generate electrode currents in behaving animals (regardless of a possibly underlying O2 modulation of COA), we have first performed recordings in freely moving animals. We simultaneously recoded from the CA1 pyramidal layer using the TACO sensor and LFP across hippocampal depth using a 32-channel linear silicon probe implanted in the proximity of the biosensor (Figure 2A). Extracellular electrophysiology allowed us to directly validate the amperometric measurement of high-frequency LFP (Figure 2—figure supplement 1). The COA signal was cleaned by subtraction of the m-PD-electropolymerized pseudo-sentinel from the Ch-sensing sites’ signal, upon frequency-domain correction of the pseudo-sentinel amplitude and phase (please see Materials and methods) (Santos et al., 2015). This procedure led to substantial removal of fast current fluctuations ascribed to LFP (example recording in Figure 2B, top). Accordingly, the median spectral power at ~1–20 Hz of the signal derived from the Au/Pt/m-PD site during both NREM sleep and wake periods decreased more than two orders of magnitude after the signal cleaning procedure (Figure 2B, bottom). The cleaned COA signal tonically changed across different brain states reaching higher values during active wakefulness and REM sleep than during NREM sleep (Figure 2—figure supplement 2). This dynamics is compatible with expected brain state-dependent ACh changes (Hasselmo and McGaughy, 2004; Marrosu et al., 1995), but remarkably, COA also fluctuated on the time-scale of seconds within brain states.

Figure 2 with 2 supplements see all
TACO sensor provides a highly sensitive and selective measurement of COA in freely behaving animals.

(A) Left panel shows a simplified diagram of the electronic circuits in freely moving recordings. The amperometric measurement (one channel for simplicity) was based on a voltage clamp circuit. Rf: feedback resistor (1 GOhm); A1, A2: OP amps; Vcmd: command voltage; Im: measured current (acquired as analog voltage signal). Vcmd is set on the MHS-BR4-VA box and sent to the head-stage. Its output is acquired by an Open-Ephys system. Electrophysiological setup is depicted in bottom. Vm: measured voltage output. The amplified LFP signal is acquired by the Neuralynx system. Right panel depicts the arrangement of a TACO sensor and a 32-channel silicon probe chronically implanted in the hippocampus of a rat. Both probes were attached to microdrives. (B) Top, segment of NREM sleep recording, a raw signal (low-pass filtered at 1 Hz), cleaned COA and confound components. Bottom, spectrum of the raw and clean COA signal during wake (n = 10) and NREM sleep periods (n = 19). Data shown as medians ± CI. (C) Average low frequency (1 Hz low-pass filtered) biosensor signals and high frequency power spectrograms triggered to SWRs detected from a silicon probe channel in CA1 pyramidal layer (top quartile of all ripples sorted by power, n = 2019). Average raw (top), cleaned (middle) COA responses and LFP spectrogram triggered on SWRs (bottom). (D) Average raw (top) cleaned COA responses (middle), average speed (middle) and LFP spectrogram (bottom) triggered to peaks in rat speed in an open-field arena (n = 127). Data are represented as mean ± CI, except in B.

Hippocampal SWRs and arousal-related locomotion bouts are discrete events associated with major phasic changes in hippocampal network activity, occurring during sleep or wakefulness respectively (Buzsáki, 2002; Buzsáki, 2015; Sirota et al., 2003). Thus, we tested whether these events could correlate with phasic COA dynamics. Hippocampal SWRs were detected from a silicon probe site in the CA1 pyramidal layer during NREM sleep. Average raw biosensor signals triggered on the peak of SWRs showed a prominent peak in all TACO sensor’s sites (Figure 2C, top). The similarity of peak amplitudes suggests an LFP-related origin of these currents, which was virtually absent in the cleaned signals (Figure, 2C, middle), and reflects the slow dynamics of the sharp wave. The high magnitude of this LFP artifact emphasizes the importance of the common-mode rejection approach in revealing COA dynamics that would otherwise be masked. Interestingly, regardless of m-PD electropolymerization, the cleaned COA signal showed a peak lagging the SWR by ~3 s. Importantly, the slow and small amplitude dip in the neurochemical confounds signal excludes the role of interferents (e.g. ascorbate or a monoamine) in the COA transient (Figure 2C, middle).

Bouts in locomotion, detected as peaks in the rat running speed, were associated with transient increases in theta power (Figure 2D, bottom) and with a transient current deflection in all TACO sensor sites (Figure 2D, top). Slow time scale currents that might have neuronal, muscle or movement artifact origin were cleaned, similar to SWRs, revealing a slow peak in COA signals following locomotion bouts, but not in the neurochemical confound signal assuring negligible contribution of these interferences in the COA signal (Figure 2D middle).

These results highlight the usefulness of our multichannel pseudo-sentinel approach to discriminate between authentic changes in COA and different sorts of current-inducing interferents, including LFP- and movement-related artifacts and neurochemical dynamics. Nevertheless, it is noteworthy that phasic changes in COA were associated with the most salient sources of non-stationarity in hippocampus, namely arousal/locomotion and SWRs (Buzsáki, 2002; Buzsáki, 2015; Sirota et al., 2003). These phenomena can potentially correlate with changes in both ChOx substrates, Ch (Norimoto et al., 2012; Vandecasteele et al., 2014; Hasselmo and McGaughy, 2004; Marrosu et al., 1995; Reimer et al., 2016; Teles-Grilo Ruivo and Mellor, 2013) and O2 (Zhang et al., 2019; Ramirez-Villegas et al., 2015), leading to transient modulation of COA. Thus, our freely moving results further emphasized the need for a detailed investigation of the O2 effect on the COA signal.

Biosensor’s COA responses to oxygen have tonic and phasic components

Since O2 is a co-substrate of oxidases, physiological O2 variations potentially affect the response of oxidase-based biosensors. However, this issue has not been thoroughly addressed in previous studies (Baker et al., 2015; Chatard et al., 2018; Dixon et al., 2002; McMahon et al., 2007; Santos et al., 2015). Here, we sought a detailed investigation of the biosensor O2-dependence in vitro, enabling the assessment of O2 effect on COA at multiple time scales (i.e. tonic and phasic dynamics). In these experiments, we took advantage of the TACO sensor configuration to sample COA and O2 dynamics from the same spot, necessary for accurate evaluation of O2-dependence of the COA signal. These recordings were performed using a new customized head-stage allowing independent control of the potential applied on each recording site. Oxygen measurement was achieved at a negative potential by O2 reduction on a gold-plated site, which was typically maximal at −0.4 V vs. Ag/AgCl (Figure 3A). In these experiments, clean COA and O2 signals were obtained by subtraction of Au/Pt/m-PD (at +0.6 V) and Au (at −0.2 V) by the pseudo-sentinel m-PD site, respectively.

Figure 3 with 2 supplements see all
Biosensors generate tonic and phasic COA components in response to O2.

(A) The voltammogram shows the DC voltage-related sensitivity of gold-plated sites toward H2O2 (n = 10) and O2 (n = 5). (B) An example of an in vitro O2 calibration. Upon removal of O2 from PBS containing 5 μM Ch, 5 μM O2 additions (arrows) were performed until the tonic sensor response saturated. (C) Representative cumulative tonic and phasic responses as a function of O2 baseline after each addition. Tonic data were fitted with Michaelis-Menten equation, resulting in a KmO2app of 0.97 μM (CI = 0.818–1.126 μM) and Imax = 13.2 pA (CI = 13.04–13.37 pA), with RMSE = 0.08 pA. Phasic responses were fitted to the Hill equation, yielding K0.5O2 = 8.6 μM (CI = 7.58–9.62 μM), Hill coefficient n = 2.31 (CI = 1.74–2.88) and Imax = 22.4 pA (CI = 20.6–24.1), with rmse = 0.50 pA. (D) K0.5O2app values of tonic and phasic components from all biosensors (n = 14). Averages and error bars are medians and CIs. Groups were significantly different (sign test, p<0.0005). (E) T90 rise times of O2 steps and COA peaks following O2 additions (n = 72–95). Bars and error bars represent medians and 95% CIs, groups were significantly different (p<0.0001, Wilcoxon rank sum test). (F) Biosensor efficiency (Ch/H2O2 sensitivity ratio) as a function of KmO2app (n = 6). For illustrative purposes, data were fitted with an exponential function. Spearman correlation between variables was −0.9 (p=0.028). (G) Maximal phasic response to O2 (from each biosensor calibration) as a function of KmO2app (n = 14, rspearman = −0.86, p<0.0001). Data were fitted with an exponential decay curve (decay constant k = 0.4 μM−1, CI = 0.031–0.78 μM−1, rmse = 0.68 μM). (H) Amplitudes of phasic ChOx responses divided into low and high-KmO2app groups (n = 7 per group) following O2 additions. Control phasic O2 using the same algorithm is plotted for the same groups (n = 6 for high-KmO2app and n = 4 for low-KmO2app). Low-KmO2app COA transients were higher than those from the high-KmO2app group (p<0.0001) and the amplitudes from both COA groups were higher than any O2 group (p<0.005). Oxygen transients from low-KmO2app vs. high-KmO2app groups did not significantly differ (p=0.998). Group comparisons by two-way ANOVA for unbalanced data followed by Tukey-Kramer post-hoc tests. Data are means ± CI. (I) T50 decay of transients from low- and high-KmO2app groups as a function of cumulative O2. The decay of peaks at 10 μM O2 was the longest (p<0.0005, two-way ANOVA for unbalanced data followed by Tukey-Kramer post-hoc tests). Data are means ± CI. (J) Hill coefficient from the fits of cumulative COA phasic component vs. cumulative O2 (as in C) as a function of KmO2app (n = 14). The two variables negatively correlate (rspearman = −0.64, p=0.015), showing an exponential-like relationship (fitted initial amplitude of 1.27 ± 0.75, decay constant of 0.33 ± 0.62 μM−1 and offset of 0.97 ± 0.67). ***p<0.001.

The in vitro tests were based on step additions of known O2 concentrations in the presence of a background Ch concentration (5 μM) representative of average brain extracellular Ch tonic levels (Brehm et al., 1987; Garguilo and Michael, 1996; Parikh et al., 2004). Importantly, unlike previous studies, this allowed us to distinguish and probe the contribution of phasic and steady-state (tonic) COA responses to O2.

Notably, upon removal of O2 from solution and in the presence of background Ch, most biosensors responded to consecutive O2 steps with a fast transient (phasic component) before reaching a steady-state (Figure 3B). Both phasic and tonic components decreased with O2 baseline, but not equally. The phasic response was usually still prominent upon the exhaustion of the tonic component (Figure 3B). We quantified these differences by fitting the Michaelis-Menten equation to the tonic changes and the Hill equation to the cumulative phasic peaks, as the latter did not appear to follow a pure Michaelis-Menten profile (Figure 3C). The resulting phasic K0.5O2 values for phasic responses were, on average, one order of magnitude larger than tonic KmO2, reinforcing that the phasic component vanishes at much larger O2 baselines than tonic responses (p<0.0005, Figure 3D). Importantly, the phasic ChOx response was not matched by a fast O2 transient following each addition, as O2 raised considerably slower than the peak in COA (Figure 3E).

Next, we assessed how the coating composition and its physical properties could modulate the sensor O2-dependence. First, we calculated the biosensor efficiency (ratio of Choline vs. H2O2 sensitivities), as a proxy to the enzyme loading in the coating and plotted it against tonic KmO2app (Figure 3F). The result revealed a decreasing trend (Figure 3F), suggesting that biosensors with a high enzyme loading have low sensitivity to tonic O2 changes (low-KmO2app). Strikingly, the biosensors with the lowest tonic KmO2app exhibited the highest phasic peaks (Figure 3—figure supplement 2), with maximal phasic responses to O2 decreasing exponentially as a function of KmO2app (Figure 3G).

In order to further detail on the effect of O2 baseline on non-stationary COA, we split the sensor calibrations into two groups according to their tonic O2-dependence. As already suggested in Figure 3E, we confirmed that the larger phasic responses in the low-KmO2app vs. high-KmO2app groups could not be attributed to differences in the underlying O2 dynamics. Oxygen transients after each addition were negligible and did not significantly differ across KmO2app groups (p=0.998, Figure 3H). Noteworthy, in the low-KmO2app group, the highest COA peak was achieved in response to the second O2 addition (10 μM of cumulative [O2]) rather than to the first in six out of seven calibrations (marginally significant difference between 5 and 10 μM O2 responses, p=0.076, paired t-test). Furthermore, the same COA peaks had the longest decay across the two KmO2app groups (p<0.0005, Figure 3I). These non-monotonic profiles with respect to [O2] reflected the deviation of the cumulative phasic COA vs. O2 curves from a Michaelis-Menten kinetics. Accordingly, the Hill coefficients extracted from calibration fittings (e.g. Figure 3C) were above two for sensors with low KmO2app and significantly decreased toward one as KmO2app increased (p<0.05, Figure 3J). These observations suggest a cooperative mechanism that enhances phasic responses as the O2 baseline increases, in biosensors with low tonic O2 dependence.

In summary, we show that, under a physiological Ch background, ChOx biosensors respond to O2 with a transient increase in enzyme activity before reaching a steady-state. Importantly, phasic and tonic components were apparently mutually exclusive and their relative magnitude was sensitive to the properties of the enzyme coating, namely enzyme loading. Sensors with high enzyme loading have a low tonic O2-dependence but show large phasic responses whose amplitude is modulated by the O2 baseline.

Modeling in vitro biosensor responses reveals the mechanisms underlying tonic and phasic oxygen-dependence

In order to provide a theoretical ground for our in vitro observations and further understand the mechanisms underlying tonic and phasic COA responses to O2, we have simulated the behavior of biosensors in calibration conditions. We numerically solved a system of partial differential equations describing the diffusion of Ch and O2 in the coating and their interaction with the enzyme, leading to H2O2 generation.

To mimic our experimental calibrations, we simulated sensor responses to 5 μM step increases in O2 (starting from zero) under a constant level of 5 μM Ch in the bulk solution. Remarkably, in line with our experimental findings, the model predicted phasic and tonic components of sensor response to O2 whose magnitude depended on O2 baseline (Figure 4A). Sensors with high enzyme loading showed higher phasic peaks and tonic responses that saturate at lower O2 baselines than sensors with a low enzyme amount (Figure 4A). To get a more resolved characterization of biosensor’s O2 dependence, we next generated response curves at 1 μM O2 steps, until saturation of enzymatic H2O2 generation was nearly reached (see Materials and methods). By simulating a range of coating thicknesses and enzyme concentrations, we found that both parameters decreased KmO2app of tonic responses (Figure 4B) and increased the magnitude of phasic peaks (Figure 4C). Interestingly, our model predicts that particular combinations of coating thickness and enzyme concentration can be used to optimize sensor sensitivity to Ch (at saturating O2 levels) (Figure 4—figure supplement 1A). Yet, such a strategy is not expected to concomitantly reduce phasic and tonic O2 dependence, which seem to be mutually exclusive. In agreement with the experimental data, our model anticipated that sensors with the lowest KmO2app exhibit the highest transient responses to O2 (Figure 4B–D). Across the simulated coatings, the highest phasic peaks occurred when sensor cumulative tonic responses were close to maximal and progressively decreased in sensor tonic saturation level as a function of tonic KmO2app (Figure 4D). This observation is in agreement with our experimental observation of highest phasic peaks at a 10 μM O2 baseline (Figure 3H–J).

Figure 4 with 2 supplements see all
Mathematical model explains ChOx-based biosensor COA responses to oxygen.

(A) Simulated calibrations of biosensors with different enzyme concentrations in the coating (coating thickness of 30 μm). Choline in bulk solution was kept constant at 5 μM and O2 was incremented in 5 μM steps from zero to 30 μM (arrows). (B) KmO2app of tonic responses as a function of coating thickness and enzyme concentration in the coating. (C) Normalized maximal phasic responses of sensors with different coating thicknesses and enzyme concentrations. Magnitudes refer to the highest phasic response divided by the maximal cumulative tonic response (Imax) from the respective simulated sensor calibration. (D) Blue trace represents the normalized maximal phasic response vs. tonic KmO2app and the orange trace refers to the level of saturation of the sensor's tonic response at which the maximal phasic peak occurs. Data were obtained from all combinations of coating thickness and enzyme concentration in B and C. (E) Concentration dynamics of Ch in the sensor coating as a function of distance from the electrode surface during a simulated calibration of a high enzyme-loaded sensor (coating thickness of 30 μm and enzyme concentration of 560 μM, same as the blue in A). Arrows indicate 5 μM O2 step increments in solution. (F) and (G) show time-courses of Ch and O2 concentration, respectively, at the electrode surface of sensors with different enzyme concentrations during simulated calibrations. Arrows indicate 5 μM O2 increments in solution. (H) Normalized profiles of phasic and tonic sensor responses as well as of concentrations of enzyme substrates and total reduced enzyme-bound complexes at the electrode surface as a function of O2 in solution. Data are from a simulated calibration of a sensor with a 30 μm coating and an enzyme concentration of 560 μM, upon 1 μM O2 step increases in solution. The ΔO2 is the initial rise in O2 following each O2 increment in solution (at a lag of 0.3 s).

To get further clues into the factors shaping sensors’ O2-evoked responses, we analyzed the concentration dynamics of Ch and O2 in the coatings during simulated calibrations. We observed that, under high enzyme loading, Ch is rapidly depleted in the coating as O2 levels in solution increase (Figure 4E). This effect is less pronounced in sensors with low enzyme loading (Figure 4F). Interestingly, significant O2 consumption, observed mainly in coatings highly loaded with enzyme, was stronger for low O2 levels before reaching saturation of the sensor tonic response (Figure 4G). These observations suggest that depletion of Ch in the enzyme coating is the limiting factor that shapes sensors’ tonic responses to O2.

To further investigate phasic responses under high enzyme loading, in addition to substrate dynamics, we assessed the levels enzyme-bound reduced intermediate species vs. buffer O2 concentration, as those are direct precursors of H2O2. We observed that, under non-saturating O2 levels, the concentrations of EredBA and EredGB change oppositely as O2 is increased, which maintains the sum of both intermediates relatively stable (Figure 4—figure supplement 1B). At 1 μM buffer O2 steps, the profiles of reduced intermediates, Ch and O2 at the electrode surface, suggest that phasic biosensor peaks result from a combination of multiple factors (Figure 4H). As expected, the sensor’s tonic response steeply decays upon Ch depletion in the coating. This decrease is accompanied by a sharp rise in the instantaneous ΔO2 at the electrode surface evoked by O2 increments in the bulk solution. As the rate of O2 consumption depends on the concentration of reduced enzyme-bound complexes, the increasing profile of ΔO2 results, in a first stage, from the depletion of the EredBA complex, followed by the decrease in EredGB (Figure 4—figure supplement 1B). In turn, the amount of EredBA and EredGB depends on both Ch and O2, which leads to a sum that is shifted to the right relative to the sensor’s tonic response. Thus, phasic sensor peaks reach maximal levels at the offset of tonic responses, when there is a combination of relatively large concentrations of the direct H2O2 precursors, namely enzyme-bound reduced complexes and O2, and very low levels of Ch in the coating (Figure 4H).

Overall, our biosensor simulations corroborate the in vitro results, firstly converging toward the notion that Ch consumption in the coating is a major factor governing sensor tonic O2 dependence. Secondly, the results suggest that non-steady state phasic responses depend on the relative concentrations of Ch, reduced intermediate enzyme complexes and O2 in the coating. This biochemical mechanism is expected to convert physiological O2 changes into spurious COA phasic signals and calls for careful investigation of the O2 modulation of COA dynamics in vivo.

Phasic COA during locomotion bouts correlates with oxygen transients

The TACO sensor offers the opportunity to probe the activity of immobilized ChOx, as a putative index of cholinergic tone, with unprecedented spatial resolution and selectivity in behaving animals. However, our in vitro data and mathematical simulations hint toward a possible confounding effect of a phasic biosensor O2-dependence on fast time-scale measurements of Ch in vivo. To directly address this question in the brain of behaving rodents, we have exploited the advantages of the tetrode configuration, ideally suited to measure multiple electroactive compounds at the same brain spot. In vivo recordings were performed using the same electrode configuration as previously described in vitro (Figure 5A, top). In this case, clean COA and O2 signals were obtained by frequency-domain-corrected subtraction of Au/Pt/m-PD (at +0.6 V) and Au (at −0.2 V) by the pseudo-sentinel m-PD site, respectively, as described in Materials and methods section.

Locomotion-related correlated changes in COA and local oxygen concentration in head-fixed mice.

(A) The schematics of the tetrode configuration used to simultaneously measure COA (H2O2) and extracellular O2.in head-fixed mice. Values on each recording site indicate the applied DC voltage vs. Ag/AgCl (+0.6 V for H2O2 and −0.2 V for O2 measurement). (B) Representative segment of simultaneous recording of COA and O2 (top), locomotion speed (middle) and LFP spectrogram (bottom). Dashed lines indicate times of detected locomotion bouts. (C) Average speed, COA and O2 signals (top) and LFP power spectrogram (bottom) triggered on locomotion bouts (n = 41 from one recording session). (D) Event counts across different categories of locomotion bouts, distinguished by the occurrence or absence of COA and/or O2 peaks. Data were collected from five recordings in three mice. (E) Amplitude of COA (shown as calibrated Ch concentrations) vs O2 transients following locomotion bouts (data from the events with increases in both signals, n = 146). Each color represents one recording session (n = 5–74 events per recording). Amplitudes were significantly correlated (rspearman = 0.77, p<0.001). (F) Spearman correlation coefficients between COA amplitudes and O2, theta power or speed (n = 5). Correlation across recordings was significant for ChOx vs O2 data (p<0.01, t-test). Each point represents a single recording session. (G) Peak lags of COA vs. O2 relative to the peak in speed (rspearman = 0.81, p<0.001, n = 146). Colors represent recording sessions (n = 5–74 events per recording). The diagonal line was plotted to ease comparison between lags. Data were obtained from five recordings in three mice and are represented as mean ± CI.

In order to obtain more controlled experimental conditions and overcome technical constraints posed by the size of the head-stage, the remaining experiments were performed in head-fixed mice spontaneously running on a treadmill (Figure 5A bottom). Strikingly, we found that phasic COA dynamics typically matched the simultaneously recorded O2 fluctuations, which were generally related to changes in behavioral state (Figure 5B). In line with the freely moving data (Figure 2D), head-fixed running bouts were temporally correlated with an increase in the power of theta oscillations as well as with delayed phasic increases in both COA and O2, peaking a few seconds later (Figure 5B and C). This lag was significantly greater than zero in all recording sessions (one-sample t-test, p<0.01), averaging 3.85 ± 2.04 s (n = 5) for COA and 5.04 ± 3.12 s (n = 5) for O2. Notably, running-related isolated COA or O2 peaks were rare, with the vast majority of the events showing either no identifiable change or co-occurrence of the two transients (Figure 5D). Amplitudes of COA peaks were significantly correlated with those of O2 (p<0.001) when pooling together the events from all recordings, which allowed sampling across the full O2 amplitude range (Figure 5E). Phasic COA correlated more consistently with O2 than with theta power or speed (Figure 5F). Moreover, running-bout-related COA and O2 peak lags were significantly correlated (p<0.001, Figure 5G) and, interestingly, within most sessions (three out of five), COA peaked significantly earlier than O2 (p=0.029, p<0.0001 and p<0.0001 for significant sessions, paired t-test).

In summary, these results indicate a strong correlation between phasic COA and O2 in the hippocampus of head-fixed mice following locomotion bouts.

Phasic COA and oxygen signals follow clusters of sharp-wave/ripples during immobility

Hippocampal SWRs are critical for memory consolidation and their occurrence has been proposed to be anti-correlated with cholinergic activity in the hippocampus (Hasselmo and McGaughy, 2004; Norimoto et al., 2012; Vandecasteele et al., 2014). However, our freely moving data showing COA transients following SWRs contradicts this prediction, posing questions on the factors driving the biosensor response during these events. Thus, we investigated whether the SWR-related response of immobilized ChOx was correlated with extracellular O2 in head-fixed mice during periods of quiescence.

Remarkably, on average, SWR events were followed by fast transients in both COA and O2 (Figure 6A). Both COA and O2 peak amplitudes correlated best with ripple power integrated over a period of ~2 s lagging them by 3–4 s (Figure 6B and C). Similarly, both SWR count and summed ripple power integrated in a 2 s window positively correlated with the delayed amplitude of both COA and O2 transients (Figure 6D–E and Figure 6—figure supplement 1).

Figure 6 with 1 supplement see all
Correlated COA and oxygen transients follow hippocampal SWRs.

(A) Average COA and O2 dynamics triggered on hippocampal SWRs (n = 1067, top) and average LFP spectrogram (bottom) from a recording session in the head-fixed setup. (B) Pseudo-color-coded Spearman correlation between COA (top) or O2 (bottom) amplitude at different time lags from SWRs (y-axis) and integrated ripple band power computed in windows of varying size (x-axis) for a representative session. White areas represent non-significant correlations (p>0.05). (C) Normalized peak correlations, obtained from the difference between maximal and minimal correlations for each integration time in B, between integrated ripple power and COA or O2. Colors represent recording sessions in different mice. (D) Average COA, O2 and ripple power dynamics triggered to SWRs bursts containing variable number of SWRs (1-3) in a 2 s window (n = 34–591 SWRs in each group, respectively from one representative session). Data are represented as median ± CI. (E) Peak amplitude of COA or O2 transients as a function of SWR burst size (as shown in D) sorted by different percentile ranges of summed ripple power. Both SWR count and total ripple power significantly affected the amplitude of COA and O2 (two-way ANOVA for unbalanced data following ART, p<0.0001 and F2,803 > 12 for both factors in COA and O2 data). (F) Amplitude of COA vs. O2 transients following SWRs from one recording session (rspearman = 0.53, p<0.0001, n = 1067). (G) Group statistics on the lags of COA and O2 peaks relative to SWRs. Each dot is the average from one recording. Bars represent means ± CI. (H) Lags of COA peaks as a function of O2 peak lags relative to SWRs. The parameters were significantly correlated (rspearman = 0.56, p<0.0001, n = 1067). (I) Summary of correlations between sensor signals and ripples. Each point represents one recording session, and error bars are CIs computed using bootstrap. Data were obtained from three recording sessions in three mice.

These findings might indicate the contribution of a time-constant related to the sensor response and/or to a relatively slow physiological process by which SWRs recruit cholinergic activity or a local hemodynamic response leading to O2 increase. Indeed, functional MRI has reported SWR-triggered increases in BOLD signal in the primate hippocampus, reflecting a local tissue hemodynamic response at a time-scale matching O2 transients observed here (Ramirez-Villegas et al., 2015). Importantly, the amplitudes of SWR-associated COA and O2 phasic transients were consistently correlated within all recordings (n = 3, p<0.001, Figure 6F). Similarly, lags of these transients to SWR were significantly correlated (Figure 6G,H,I).

Together, the data indicate correlated phasic changes in COA and extracellular O2 in response to SWRs (Figure 6I), especially when they happen in clusters. As in the case of locomotion bouts, at this stage one could not rule out the contribution of neither phasic Ch nor O2 as the trigger for the peaks in COA. The putative cholinergic origin of this response would, nevertheless, be surprising given the suppressive effect of ACh on SWR occurrence (Norimoto et al., 2012; Vandecasteele et al., 2014). Thus, in light of the O2 transients that accompany the rise in COA and of our in vitro findings of the phasic O2 dependence in this type of biosensors, these observations cast doubt on the validity of the putative SWR-triggered cholinergic response.

Interactions between COA and oxygen are not sensitive to ongoing hippocampal dynamics and depend on the time-scale

The correlation between COA and O2 following locomotion and SWRs may reflect interaction between the two signals or result from the coincident recruitment of cholinergic and hemodynamic responses. While a consistent relationship between the two variables is expected in the first case, irrespective of ongoing network dynamics, the same may not happen in the latter. To get insights into this question, we analyzed an additional category of events, consisting of fast O2 transients detected outside the time-windows surrounding SWRs and peaks in locomotion. Remarkably, virtually all (>95%) of these events had an associated COA transient. The dynamics of COA and O2 were typically similar (Figure 7A), with COA peaks lagging, on average, from −0.09 s to 0.48 s (n = 3 recordings) relative to O2. Signal amplitudes were significantly correlated both when pooling together all events (p<0.0001, Figure 7B) or within all individual sessions (p<0.006). The amplitude correlation coefficients were in the range of those obtained for O2 peaks associated with locomotion and SWRs (Figure 7C), but the average amplitudes of O2 and corresponding COA signals were in the sub-micromolar range, comparable to those associated with SWRs (Figure 7D). Overall, although the amplitude range of locomotion-related peaks was wider, it is notable that the whole data fit to a COA/O2 relationship that seems to follow the same model across recordings and event types (Figure 7D). These observations are therefore compatible with an interaction between COA and O2, although contribution of third-party factors could not be definitely excluded at this stage.

Figure 7 with 1 supplement see all
Correlation between COA and spontaneous O2 transients suggests O2-COA directionality.

(A) Average COA and O2 dynamics triggered to fast O2 transients (duration ~5 s) detected outside periods when SWRs or locomotion bouts occurred. Data is from one recording session (n = 42 events). (B) Relationship between amplitudes of O2 and associated COA transients outside SWRs/locomotion bouts. Colors represent different recording sessions (n = 10–45 from three recordings, rspearman = 0.58, p<0.0001). (C) Group summary of COA vs. O2 amplitude correlations under different behavioral and/or electrophysiological contexts. (D) COA vs. O2 amplitude across animals and states. Each point represents the median of events from a recording session (error bars are CIs). Data were fitted to the Michaelis-Menten equation (Vmax = 1.58, Km = 4.84). (E) Lag of COA peaks relative to O2 peaks (red) and slope of COA vs. O2 amplitude (blue) for O2 transients with varying rise time for a single session; medians with CIs (left). Right shows the relationship between amplitudes of O2 and associated COA transients for the O2 rise time corresponding to largest slope marked with * on the left panel. (F) Group statistics on maximal COA lags, lags at maximal COA/O2 slope and lags associated with longest O2 transients (ANOVA, F2,12 = 15.22, with post-hoc Tukey test, p=0.51 for max COA lag vs. lag at max COA/O2, p=0.0006 for max COA lag vs. lag at max O2 rise and p=0.0038 for lag at maximal COA/O2 slope vs. lag at max O2 rise). (G) Group statistics on O2 rise times corresponding to maximal COA lags and COA/O2 slope in (E). Values from both groups were significantly lower than the longest O2 rise time observed in a given recording (p<0.0001, one-sample t-test). (H) Group statistics on the slopes of COA vs. O2 amplitude at its maximum value and at maximum O2 rise. Differences between groups were significant (p=0.002, paired t-test). ***p<0.001, **p<0.01. Data were obtained from five recording sessions in three mice and are represented as mean ± CI, except in D.

Besides the amplitude of O2 transients, the temporal profile of O2 rise might influence the shape and amplitude of associated ChOx responses and thus provide further hints on the causality and directionality of COA-O2 interactions. This analysis is of particular relevance in light of the phasic component of biosensor’s O2 dependence that we have uncovered by our in vitro tests and mathematical modeling. In vivo we detected spontaneous O2 peaks at different frequency bands (ignoring their correlation with SWRs or speed), resulting in a spectrum of O2 rise times from less than 2 to 14 s (please see Materials and methods section for details). Across all recording sessions, we consistently observed that the COA peak anticipated O2 (negative lag) as O2 rise time increased (Figure 7E,F and Figure 7—figure supplement 1A). Maximal COA lags averaged 0.38 ± 0.42 s and were associated with fast O2 rises, lasting 1.4–2.6 s (Figure 7F and G). Furthermore, the slope of COA vs. O2 amplitudes was time-scale dependent, peaking for O2 transients that took 2.3 to 6.2 s to rise (Figure 7E,G and Figure 7—figure supplement 1B) and progressively decreasing for longer O2 rises (Figure 7E,H and Figure 7—figure supplement 1B). The time-scale dependence of the COA vs. O2 slope is apparently related to the non-linear interaction between COA and O2, as a function of O2 rise time. Contrasting with the approximately linear increase in O2 amplitude as a function of O2 rise (Figure 7—figure supplement 1C), the amplitude of corresponding COA peaks was, on average, nearly time-scale independent for O2 rise times in the range of 3–14 s (Figure 7—figure supplement 1D).

These results provide important insights into the interaction between COA and extracellular O2 in the hippocampus. The advancement of COA relative to O2 could be compatible with a Ch-O2 directionality, possibly caused by ACh-evoked changes in local blood flow (Takata et al., 2013). However, the diffusional delay underlying such a mechanism is expected to be constant as a function of time-scale. Moreover, the time-scale dependence of the relative amplitude of enzyme response is hard to interpret in light of a Ch-O2 directionality. In contrast, these in vivo observations are fully compatible with the phasic component of biosensor’s O2 dependence that we have found in vitro and supported by a mathematical model of the sensor. In agreement with in vivo interplay between COA and O2, the discovered phasic O2 dependence predicts that both tonic (or slow) and phasic changes in O2 would evoke a transient increase in COA preceding the peak in O2. Such modulation is expected to be time-scale-dependent, being maximal at relatively short time-windows. Additionally, in line with in vivo COA and O2 dynamics, this phenomenon predicts a continuous drift in COA-O2 peak lags as O2 rise time increases.

Together, these results converge to the hypothesis that the observed changes in COA are mainly caused by phasic non-steady-state responses of ChOx to O2 fluctuations in vivo.

Exogenous oxygen transients in the hippocampus elicit phasic COA responses

Our correlational analysis in vivo, supported by in vitro characterization of biosensor O2 dependence and mathematical modeling of the biosensor, points toward an effect of O2 transients on phasic COA when recording from the hippocampus. We tested the causality of this interaction first by evoking changes in O2 by local application of small volumes of O2-saturated saline through a glass micropipette, positioned at a few hundred microns from the biosensor tip. To evoke different O2 dynamics, we varied ejection parameters such as time and pressure.

Remarkably, immobilized ChOx exhibited robust phasic responses to exogenous O2 transients, regardless of the time-scale of O2 change (Figure 8A and Figure 8—figure supplement 1A). Amplitudes of COA and O2 were significantly correlated (p<0.0001, Figure 8B). However, similarly to spontaneous dynamics, the COA/O2 amplitude ratio of single events (equivalent to COA/O2 slope in spontaneous data) significantly decreased with O2 rise time in all experiments (negative correlation, p<0.05, Figure 8C). The decrease in COA amplitude was accompanied by the advancement of its peak relative to O2 (p<0.001 in all recordings, Figure 8D). Thus, these results qualitatively recapitulate our observations for the spontaneous COA-O2 interactions, reinforcing the putative O2-COA directionality.

Figure 8 with 1 supplement see all
Exogeneous O2 elicits phasic COA responses in the hippocampus in vivo.

(A) Representative examples of slow and fast O2 transients and associated COA responses evoked by local application of exogenous O2 from a glass micropipette. (B) Amplitudes of COA vs. locally evoked O2 transients. Data are from three recordings (color-coded, n = 13–40 per session). Amplitudes were significantly correlated when pooling all data (rspearman = 0.81, p<0.0001) and within two sessions (p=0.0017 and p<0.0001, analysis not performed in one session due to the narrow range of O2 amplitudes covered). (C) Ratio of COA vs. evoked O2 peak amplitudes as a function of O2 signal rise time. Colors denote recording sessions and trendlines are linear fits performed on data from each session. In all cases, the variables were negatively correlated (p=0.037, p<0.0001, and p<0.001 for each session). (D) Lag of COA relative to locally evoked O2 transient peaks as a function of O2 rise time. Lags significantly decreased with O2 rise time in all sessions (rspearman for each recording ranged from −0.76 to −0.88, p<0.001). (E) Representative traces showing slow and fast transients evoked by O2 inhalation. E-H plots are analogous to A-D, but for O2-inhalation-induced O2 transients. (F) Amplitudes of COA vs. O2 transients. Data were obtained from three recordings (color-coded, n = 8–40 per session). Whole data as well as data from individual experiments were significantly correlated (p<0.0001 and p<0.0005, respectively). (G) Amplitude ratio of COA vs. O2 transients as a function of O2 rise time. Variables were negatively correlated (red and yellow sessions, with rspearman of −0.66 and −0.86 respectively, p<0.0001 and p=0.011). (H) Lags of COA relative to O2 peaks significantly decreased as a function of O2 rise time (red and yellow sessions, rspearman of −0.58 and −0.90 respectively, p<0.0001 and p=0.0046). Analysis in F-H not performed in one session (blue dots) due to insufficient coverage of O2 amplitudes and rise times. (I) Summary of amplitude and COA-O2 lags correlations for COA and O2 transients evoked by local O2 application and O2 inhalation. Data were obtained from six recording sessions in five mice and are presented as means ± CIs.

Next, we manipulated hippocampal O2 levels non-invasively, through inhalation, to further confirm O2 causality. We exposed mice to a pure O2 stream during variable periods (4–30 s) in order to generate different O2 transients. Like in the case of local O2 delivery, we observed reproducible changes in COA in response to exogenous O2 (Figure 8E and Figure 8—figure supplement 1). The data showed a significant correlation between peak amplitudes (p<0.0001, Figure 8F). Importantly, both the COA/O2 amplitude ratio and COA peak lag significantly decreased as a function of O2 rise time (p<0.05), corroborating the conclusions from local O2 manipulation.

Overall, O2 inhalation tended to generate slower and smaller O2 transients (and perhaps more physiological) than those by local application. Despite the differences in magnitudes and time-scales, the tested correlations are consistent across the two paradigms (Figure 8I) as well as with the spontaneous interactions between COA and O2 (Figure 7E–H). Both spontaneous and evoked O2-COA interactions are therefore fully compatible with our in vitro characterization of a novel phasic O2-dependence of this type of sensors caused by non-steady-state enzyme activity in response to O2. Our results converge to the conclusion that the putative cholinergic transients observed in the hippocampus of behaving rodents rather result from phasic modulation of COA by physiological O2 fluctuations.

Discussion

We have developed a novel multi-site tetrode-based amperometric ChOx (TACO) sensor optimized for the highly sensitive and unbiased simultaneous measurement of immobilized ChOx activity (COA), as an index of cholinergic activity, and O2 dynamics in the brain. Our approach, based on the differential plating of recording sites to create pseudo-sentinel channels outperforms previous common-mode rejection strategies, which were limited by large distance between sensor and sentinel sites, impedance mismatch and diffusional cross-talk (Burmeister et al., 2003; Parikh et al., 2007; Santos et al., 2015). This strategy allowed us to substantially reduce the size and increase the spatial confinement of recording sites by using a 17 μm wire tetrode as the biosensor electrode support. Our recordings in freely moving and head-fixed rodents reveal the usefulness of this compact multi-site design to clean artifacts from sensor signals and assess the correlation between the activity of the immobilized enzyme and brain extracellular O2 on a fast time-scale. Importantly, this method can be generalized to improve the selectivity and address the in vivo O2-dependence of any oxidase-based biosensor.

By simultaneously measuring COA and extracellular O2 in the hippocampus of behaving mice, we found that fast biosensor signals correlate in amplitude and time with O2 transients evoked by behavioral and network dynamics events exemplified by locomotion bouts and SWRs. Notably, the relationship between COA and O2 dynamics was apparently not sensitive to the underlying neurophysiological or behavioral context and was preserved during periods without appreciable SWR incidence or locomotion. By using two different methods to manipulate extracellular O2, we show that O2 fluctuations in the physiological range can drive phasic COA. Remarkably, the time-scale dependence of biosensor response amplitude and lag relative to exogenous O2 qualitatively matched that of spontaneous dynamics, suggesting that the same directionality takes place in the spontaneous conditions.

Locomotion-related O2 elevations in head-fixed mice have been recently shown to be modulated mainly by respiration rate (Zhang et al., 2019), whereas SWR-evoked O2 peaks have been indirectly inferred by fMRI and likely result from neurovascular coupling (Leithner and Royl, 2014; Ramirez-Villegas et al., 2015). Thus, our study provides a link between the neurophysiological or systemic mechanisms that modulate brain O2 levels and the response of ChOx-based biosensors in vivo. As it is an intrinsic component of any behavioral task, our results highlight the importance of controlling for O2-evoked biosensor signals related to locomotion or movement. It is thus likely that, in reward-related tasks, locomotion related to reward retrieval elicits few seconds delayed phasic changes in O2 that drive transient increases in COA. Likewise, a high incidence of SWRs in reward locations, reflecting a consummatory state (Buzsáki, 2015), might trigger O2 transients and, in turn, phasic ChOx biosensor responses. These two examples provide alternative explanations for previously reported cholinergic transients inferred from COA signals in the prefrontal cortex and hippocampus of rodents engaged in cognitive tasks (Howe et al., 2017; Parikh et al., 2007; Teles-Grilo Ruivo et al., 2017). Importantly, since the rate of the enzymatic reaction depends on both substrates, biosensor COA responses caused by O2 transients are expected to decrease following experimental controls that have been aimed at inhibiting or removing cholinergic inputs, making this control an inadequate demonstration of the nature of COA signal (Parikh et al., 2007).

Our in vitro characterization of the biosensor O2 dependence provided critical insights to interpret the in vivo relationship between COA and O2. We found robust O2-evoked phasic responses whose amplitude was anti-correlated with sensors’ tonic O2 dependence. Interestingly, the phasic peaks decreased with O2 baseline, but were detected even under relatively high O2 levels, suggesting a high likelihood of such responses to occur in vivo. Thus, our data emphasize the impact of O2-evoked non-steady-state biosensor dynamics on fast time-scale in vivo measurements. This has not been described in previous studies partly because the experimental procedures used to study O2-dependence were unable to unmix tonic and phasic components of sensor response (Baker et al., 2017; Burmeister et al., 2003; Dixon et al., 2002). Instead of generating a continuous O2 increase, we induced step increments in O2, allowing temporal deconvolution of sensor response components and unbiased estimation of the corresponding K0.5O2app values.

Remarkably, most of our observations related to sensor O2-dependence were predicted by mathematical simulation of biosensor responses in vitro. The model incorporated the kinetics of the enzyme-catalyzed reaction, including enzyme-bound intermediate species, and simulated substrates’ diffusion into the coating and interaction with the enzyme leading to H2O2 formation. In agreement with the experimental data, the model predicted that tonic and phasic components of O2 dependence are mutually exclusive. Furthermore, our simulations suggest that increasing the enzyme loading, either by changing enzyme concentration or the coating thickness, amplifies sensor phasic responses and reduces tonic KmO2app. It is therefore apparent that there is no perfect combination of these two parameters that can concomitantly minimize the two components of biosensor O2 dependence. In addition to reinforcing our experimental conclusions, the biosensor model provided important insights into the factors that determine phasic and tonic components of O2 dependence. Simulations showed that tonic responses to O2 are largely shaped by Ch depletion in the coating, which limits the linearity of the sensor response. On the other hand, phasic responses depended on the instantaneous balance between the concentration of substrates and reduced enzyme-bound intermediates, which lead to the formation of H2O2. Simulated transients were highest when the concentration of reduced intermediates and O2 were in relative excess in comparison to Ch. Since variations in enzyme concentrations, coating thickness and diffusional components have mainly a quantitative effect in our estimates, these conclusions can be extrapolated to sensor geometries and enzyme coating compositions that we have not covered. Furthermore, the mathematical model of the biosensor described here provides a rigorous approach for exploration and optimization of the design of any future enzymatic biosensors and investigation of their behavior under non-stationary in vivo conditions.

The phasic O2-evoked COA signals described in vitro and predicted by mathematical modeling provide crucial information to interpret the time-scale dependence of in vivo sensor dynamics following either spontaneous or exogenously evoked O2 peaks. In light of those results, the temporal advancement and amplitude drop of biosensor transients relative to O2, as O2 rises for longer periods, is compatible with a major contribution of the phasic component of biosensor’s O2 dependence. This observation suggests that, in vivo, our biosensors operated in a regime close to saturation of the tonic response and highlight the effect of non-steady-state ChOx biosensor responses to O2, which can be erroneously attributed to Ch.

Our observations disfavor the quantitative optimization of coating properties as a strategy to reduce sensors’ O2 dependence. Instead, we anticipate that strategies that increase O2 accumulation in the enzyme coating (Njagi et al., 2008) might have relative success, although the high O2 levels required to cancel phasic responses are hard to reach passively. Alternatively, some oxidase-based biosensors for in vitro applications have incorporated an electrochemical actuator that enables local manipulation of O2 concentration based on water electrolysis (Park et al., 2006). However, applying such design in vivo would require miniaturization and separation between the O2 generation compartment and the brain to avoid electrolytic tissue damage. Furthermore, in addition to the main O2 confound, one cannot completely rule out a possible modulation of COA in vivo by factors that affect enzyme conformation, including temperature and pH (Hekmat et al., 2008). Although the enzyme is poorly sensitive to the modest variations of these factors in vivo (Csernai et al., 2019; Venton et al., 2003), it would be relevant to characterize their potential contribution to COA dynamics in future studies. A further validation of ChOx-based measurements could be achieved by confronting the dynamics of COA with that of cholinergic signals measured with other sensing approaches, under the same experimental conditions. The latter technics include optogenetically taged single unit recordings or fluorescence reporters, which have previously revealed fast cholinergic dynamics related to arousal, sensory sampling, negative reinforcements, and unexpected events (Eggermann et al., 2014; Hangya et al., 2015; Lovett-Barron et al., 2014; Reimer et al., 2016).

In summary, our results suggest that ChOx biosensor signals in vivo are composed of a mixture of O2-related artifacts and true cholinergic dynamics. The weight of each factor depends on the time-scale, with slow state-related changes reflecting cholinergic dynamics with low O2-related contamination and fast transients, except for a minor fraction (less than ca. 5% in our data), being caused by phasic O2 fluctuations. We show that O2 transients can be triggered by cognitively relevant events, such as locomotion and periods of high SWR incidence, and confound the ChOx-based measurement of cholinergic activity. Thus, our study reveals a previously ignored phasic O2-dependence of ChOx-based biosensors, which is critical to control for, particularly in the case of fine time-scale measurements of ACh. Importantly, the extent of the interplay between COA and O2, and its non-linear dependence on the dynamics of enzyme substrates’ concentrations at multiple time-scales made extraction of authentic phasic Ch from the in vivo COA signal unfeasible.

Our conclusions, supported by a generic mathematical modeling, can be generalized to other oxidase-based biosensors that have been used to measure neurotransmitters or metabolically relevant molecules in the brain (Chatard et al., 2018; Dixon et al., 2002; Hascup et al., 2013; McMahon et al., 2007). The exact extent of phasic and tonic O2 dependence would depend on the particular enzyme kinetics and on the basal extracellular concentrations of analyte relative to the magnitude of changes in the brain.

Materials and methods

Key resources table
Reagent type (species) or resourceDesignationSource or referenceIdentifiersAdditional information
Chemical compound, drugChitosanSigma-Aldrich448869
Chemical compound, drugChloroplatinic acid hydrateSigma-Aldrich520896
Chemical compound, drugCholine chlorideSigma-AldrichC7017
Chemical compound, drugCholine oxidase from Alcaligenes sp.Sigma-AldrichC5896
Chemical compound, drugDopamine hydrochlorideSigma-AldrichH8502
Chemical compound, drugGold chloride solutionSigma-AldrichHT1004
Chemical compound, drugHydrogen peroxideSigma-Aldrich216763
Chemical compound, drugm-PhenylenediamineSigma-AldrichP23954
Chemical compound, drugp-BenzoquinoneSigma-AldrichB10358
Chemical compound, drugSodium-L-AscorbateSigma-AldrichA7631

Chemicals and solutions

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All chemicals were of analytical grade, purchased from Sigma-Aldrich and used as received. Solutions were prepared in ultra-pure deionized water (≥18 MΩ.cm) from a Milli-Q water purification system.

Tetrode fabrication and platings

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The microelectrode support material was a 17 μm diameter Platinum/Iridium (90/10) wire insulated by a polyimide coating (California Fine Wire Company). Tetrodes were fabricated using standard methods (Gray et al., 1995). Briefly, four wires were twisted together and heated to melt the insulation, creating a stiff bundle of twisted wires with a total diameter of approximately 60 μm. The wires’ insulation at the untwisted ending of the tetrode was then removed and the tetrode was inserted in a silica tube (150 μm inner diameter), which was glued to a holder that allowed easy manipulation of the tetrode. Next, the untwisted endings of the tetrode wires were soldered to the pins of an adapter fixed to the tetrode holder, allowing connection to the potentiostat’s head-stage. Finally, the twisted ending of the tetrode was cut using micro-serrated stainless-steel scissors, leaving 1–2 cm of tetrode wire protruding out of the silica tube.

Tetrode surface treatments and platings were performed with a portable potentiostat (EmStat 3, PalmSens BV), using a freshly prepared Ag/AgCl wire (125 μm diameter, WPI inc) as pseudo-reference electrode. Prior to platings, electrode surfaces were cleaned by swirling the tetrode tip in isopropanol followed by an electrochemical treatment in PBS. For that purpose, we applied 70 cycles of a square wave with a first step at +1.2 V for 20 s followed by a 4 s step at −0.7 V. All tetrode sites were then gold-plated in a 3.76 μM aqueous solution of tetrachloroauric acid by applying 20 cycles of a square wave that alternated between +0.6 V for 10 s and −1.0 V for 10 s. Sites’ impedances were checked after gold-plating using a nanoZ impedance tester (Multichannel Systems, GmbH). The pair of sites with the highest impedance was then platinized in a 10 mM chloroplatinic acid solution in 0.1 M sulfuric acid by DC amperometry at −0.1 V, until a current of −30 nA was reached.

TACO sensor coatings

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Choline oxidase immobilization was performed as previously described (Santos et al., 2015). Briefly, a 0.5% (w/v) chitosan stock solution was solubilized in saline (0.9% NaCl) under stirring at pH 4–5, adjusted by addition of HCl. After solubilisation, the pH was set to 5–5.6 by stepwise addition of NaOH.

To form a chitosan/ChOx cross-linked matrix, 1.5 mg of p-benzoquinone was added to 100 μL of 0.2% chitosan. Four μL of this solution were then mixed with a 4 μL aliquot of ChOx at 50 mg/mL in saline. The tetrode tip was coated by multiple dips (10-15) in a small drop of ChOx immobilization mixture, created using a microliter syringe (Hamilton Co.). The microelectrode and syringe were micromanipulated under a stereomicroscope. The coating procedure was stopped when the chitosan/protein matrix was clearly visible under the microscope.

Following enzyme immobilization, tetrode site’s response to Ch was tested and meta-phenylenediamine (m-PD) was electropolymerized on the pair of sites with the highest sensitivity (please see Biosensor calibrations sub-section). Electropolymerization was performed in a nitrogen bubbled oxygen-free PBS solution of 5 mM m-PD by DC amperometry at +0.6 V during 1500s. The biosensors were stored in air and calibrated on the day after m-PD electropolymerization.

Biosensor calibrations

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All in vitro tests were done in a stirred calibration buffer kept at 37°C using a circulating water pump (Gaymar heating/cooling pump, Braintree Scientific, Inc, USA) connected to the calibration beaker. Routine calibrations after enzyme immobilization and m-PD electropolymerization steps were performed by amperometry at a DC potential of +0.6 V vs. Ag/AgCl pseudo-reference electrode. After stabilization of background current in PBS, sensors were calibrated by three consecutive additions of 10 µM Ch followed by 4.9 µM H2O2. In the case of complete (m-PD polymerized) biosensors, the response to 1 µM DA and 100 µM AA was also tested. Voltammograms of H2O2 were done by consecutive additions of 4.9 µM H2O2 at different applied DC voltages.

In vitro O2 tests were carried out in a sealed beaker. After addition of 5 µM Ch, the calibration buffer was bubbled with nitrogen during approximately 30 min. Then, known O2 concentrations (5 or 10 µM) were added to the medium from an O2-saturated PBS solution previously bubbled with pure O2 during 20 min. Biosensor response to O2 additions was measured at +0.6 V vs. Ag/AgCl. In a set of calibrations, O2 was concurrently measured by polarizing a gold-plated channel at negative voltage, while keeping the remaining at +0.6 V. The electrode response to O2 was characterized by obtaining O2 voltammograms by calibrations that consisted in three additions of 5 µM O2, performed at different applied DC potentials (Figure 3A). In these experiments, O2 was purged from the solution after each voltage step. Although the gold-plated site could also reduce H2O2, the very low response magnitude as compared to O2 (Figure 3A) assured a negligible contribution of H2O2 generated by immobilized ChOx to the O2 signal. To avoid electrical cross-talk between tetrode sites, sporadically observed when we applied a high negative voltage to one site, we set −0.2 V vs. Ag/AgCl as the standard voltage for O2 measurements both in vitro and in vivo. At this holding potential, neither current cross-talks nor a detectable effect on local O2 levels (that could affect COA signal) were observed (Figure 3—figure supplement 1).

Sensor response times to Ch and O2 were estimated using the above-mentioned calibration setups.

Experimental model and subject details

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Freely moving recordings were performed on a 6 months old Long-Evans rat and head-fixed recordings were done in a total of six 3–7 month old C57BL/6J mice. Variability from posthoc characterization of the sensor performance, as well as from inferred electrode localization made sample size predetermination unreliable. We set a minimal biological sample size of 3 for any analyzed parameter, which was found to be appropriate given the consistency of the in vivo data. Additionally, we performed a large sample study in vitro. All experimental procedures were established, and have been approved in accordance with the stipulations of the German animal welfare law (Tierschutzgesetz)(ROB-55.2–2532.Vet_02-16-170).

Surgeries

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For freely moving recordings, we chronically implanted a tetrode biosensor and a 32-channel linear silicon probe array (A1 × 32-7mm-100–1250 H32, NeuroNexus Technologies, Inc) in the rat brain. The general procedures for chronic implantations of electrode arrays have been described in detail (Vandecasteele et al., 2012). Prior to surgery, the tetrode biosensor and the silicon probe array were attached to home-made microdrives. Silicon probe’s sites were then gold-plated until impedances at 1 kHz decreased below 200 kΩ (Ferguson et al., 2009). Anesthesia was induced with a mixture of Fentanyl 0.005 mg/kg, Midazolam 2 mg/kg and Medetomidine 0.15 mg/kg (MMF), administered intramuscularly. The rat was continuously monitored for the depth of anesthesia (MouseStat, Kent Scientific Corporation, Inc). After the MMF effect washed out, anesthesia was maintained with 0.5–2% isoflurane via a mask, and metamizol was then subcutaneously administered (110 mg/kg) for analgesia. The tetrode biosensor was implanted in the cortex above the right dorsal hippocampus (AP −3.7 mm, ML −2.5 mm, DV −1.2 mm, relative to bregma) and the silicon probe array was implanted at 0.8 mm posterior from it, spanning most cortical and hippocampal layers (AP −4.5, ML −2.4, DV −3.4). The microdrives were secured to the skull with a prosthetic resin (Paladur, Kulzer GmbH). An Ag/AgCl (125 µm thick) silver wire coated with Nafion (Hashemi et al., 2011) was inserted in the cerebellum and served as the pseudo-reference electrode for electrochemical recordings. The ground for electrophysiology was a stainless-steel screw implanted at the surface of the cerebellum. To reduce line noise, this Ag/AgCl wire was shorted with the electrophysiology ground at the input of the electrochemical head-stage.

Mice used in head-fixed recordings were implanted with a head-post. Anesthesia followed the same procedures as in rats. A mixture of 0.05 mg/kg Fentanyl, 5 mg/kg Midazolam, and 0.5 mg/kg Medetomidine was administered intraperitoneally to induce anesthesia, which was later maintained with isoflurane and Metamizol (200 mg/kg). A craniotomy was made above the dorsal hippocampus and a Nafion-coated Ag/AgCl wire was implanted in the cerebellum. Depending on the head-post configuration, it was cemented either to the back of the skull above the cerebellum or above the hemisphere contralateral to the craniotomy, using UV-curing dental cement (Tetric EvoFlow, Ivoclar Vivadent AG). Finally, the craniotomy and surrounding skull were covered with a silicone elastomer (KWIK-CAST, World Precision Instruments Inc).

Electrochemical and electrophysiological equipment and recordings

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Amperometric measurements were performed using either a four-channel (MHS-BR4-VA) or a 8-channel (MBR08-VA) potentiostat connected to four- or eight-channel miniature head-stages, respectively (npi electronic GmbH, Germany). In addition to providing a higher channel count, the MBR08-VA allowed independent control of the potential applied to each channel. This feature enabled simultaneous measurement of the biosensor signal, arising from ChOx, and O2. The DC analog signal from the head-stage was amplified and digitized at 30 kHz and stored for offline processing using the Open Ephys acquisition board and GUI (Siegle et al., 2017).

Freely moving electrochemical recordings were done using the MHS-BR4-VA potentiostat and the corresponding four-channel miniature head-stage. Electrophysiological signals were pre-amplified using a 32-channel head-stage with 20x gain (HST/32 V-G20, Plexon Inc) which was connected to a multichannel acquisition system (Neuralynx Inc). Data was acquired at 32 kHz and stored for offline processing. Both head-stages were connected to the respective recording systems via light and flexible cables suspended on a pulley so as not to add weight to the animal’s head. The tetrode biosensor was gradually lowered through the cortex until it reached the hippocampal CA1 pyramidal layer. Correct targeting was assessed based on brain atlas coordinates and by the identification of hippocampal ripples. Recordings were performed in a square open-field arena (1.5 m x 1.5 m), where the animal could sleep or explore the environment at will. Chocolate sprinkles were occasionally spread on the maze to enforce exploratory behavior. The position of the rat head was derived from small reflective markers attached to the chronic implant. A motion capture system consisting of multiple infrared cameras (Optitrack, NaturalPoint Inc) was used to 3D-track the markers with high spatio-temporal resolution (data acquired at 120 Hz).

Head-fixed recordings in mice were performed using the MBR08-VA potentiostat and respective head-stage. After fixing the mouse, the layer of silicone elastomer protecting the craniotomy was removed. The dura matter above the target brain region was removed and the tetrode biosensor was slowly inserted through the cortex until the hippocampal CA1 pyramidal layer was reached. Accurate targeting was assessed according to brain atlas coordinates and/or by the online identification of hippocampal ripples in the recording. In 5 out of 10 recording sessions mice were head-fixed on a cylindrical treadmill. Movement was quantified based on the video optical flow arising from treadmill rotation using Bonsai (Lopes et al., 2015). In the remaining sessions, mice were head-fixed on a rotating disc which encoded its turns. The analog signal from the disc encoder was fed into the Open Ephys acquisition board and used to quantify mice locomotion.

Data analysis

Raw recordings were preprocessed by low-pass filtering and resampling at 1 kHz. All data analysis was done in Matlab using custom-made functions (MathWorks).

In vitro sensor responses

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In vitro analysis of biosensor responses was performed on 10 Hz downsampled data, low-pass filtered at 1 Hz. Sensitivities to Ch and O2 were determined by linear-regression of the responses to the first three analyte additions, whereas the sensitivities to H2O2 and interferents were estimated from a single addition. Following pseudo-sentinel subtraction (Au/Pt/m-PD - Au/m-PD sites), selectivity ratios for the COA measurement were estimated by dividing the mean of Ch sensitivity by the mean of interferent responses. The biosensors’ limit of detection (LOD) for Ch, extracted from the COA signal, was calculated as the Ch concentration corresponding to three times the baseline standard deviation (SD). The T50 and T90 response times were defined as the time between the onset of current increase in response to analyte and 50% or 90% of the maximum current, respectively.

Artifact cancellation by common-mode rejection

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In vivo electrochemical signals from sites sensitive to COA were cleaned by subtraction of the respective 1 kHz data by the corresponding pseudo-sentinel channel upon a frequency-domain correction. The latter procedure has been described in detail and optimizes common-mode rejection by correcting phase and amplitude mismatches between channels arising from slight frequency-dependent variations in impedance (Santos et al., 2015), a procedure conceptually analogous to orthogonalization of the EEG signals (Hipp et al., 2012). This correction was based on the estimation of a transfer coefficient (T) describing the transfer function from the currents derived from the pseudo-sentinel to the COA-sensitive channel in the frequency domain, according to the following equation:

(1) COAmPD=iFFT(Au/Pt/mPD(jw)T(jw)Au/mPD(jw)),

where jw is a complex value at frequency w and COAmPD is the clean COA signal obtained from sites with m-PD (note that COAmPD was used as the measure of COA dynamics throughout this study).

Upon applying a fast Fourier transformation (FFT), each signal can be described by its amplitude and a phase across a range of frequencies. The amplitude of T for each FFT frequency bin was then estimated from the square root of ratio between the power of the platinized channel carrying the COA signal and the pseudo-sentinel channel. The phase of T was estimated from the phase shift of the cross-spectrum, reflecting the difference between the phases of the two signals at each frequency, during time-windows with high phase-locking (>0.9) in each frequency bin (corresponding to periods of high consistency of phase-shift values). These estimates were computed for each biosensor used in vivo from average spectra obtained from multiple slow-wave periods devoid of movement artifacts. In the low-frequency range (<0.3 Hz), due to COA contribution to power in the platinized channel, the estimation of the amplitude of T was done by linear extrapolation considering the trend at contiguous higher frequencies. The cleaned COA signal was obtained by inverse FFT of the corrected subtraction in the frequency domain. Cleaned O2 signals were obtained following the same logic:

(2) O2=iFFT(Au0.2V(jw)T(jw)Au/mPD(jw)),

where Au-0.2V represents the O2 measuring channel polarized at −0.2 V vs. Ag/AgCl whereas the Au/mPD channel (at +0.6 V vs. Ag/AgCl) was used as the pseudo-sentinel. To substantiate the validation of the in vivo COA measurement, two additional cleaned signals were computed:

(3) COAnonmPD=iFFT(Au/Pt(jw)T(jw)Au(jw)),
(4) NCC=iFFT(Au(jw)T(jw)Au/mPD(jw)),

The COAnon-mPD signal results from the pair of sites lacking m-PD, whereas the neurochemical confounds signal (NCC) represents the dynamics of the mixed contribution of neurochemical confounds (e.g. ascorbate, dopamine) picked-up by the Au site.

The cleaned signals (Equations 1-4) were then low-pass filtered at 1 Hz and downsampled to 10 Hz for most of the analysis excluding time lags, which were computed on 100 Hz downsampled data.

Brain state separation

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Local-field potential-related power spectrograms were computed using custom-made Matlab functions based on multi-taper analysis methods (Mitra and Pesaran, 1999). Separation of brain states in freely moving recordings was based on LFP spectral features and behavior. Active wake states were defined as periods when the animal moved vigorously and continuously (>30 s) and showed a prominent LFP spectral peak in theta range (6–10 Hz). Quiet wakefulness or immobility was defined as a period without prominent theta and with occasional movements (<30 s between movement bouts). Long periods (>1 min) without movement and without prominent theta, rather showing high delta power (1–4 Hz) were ascribed to NREM sleep. Rapid eye movement sleep was detected as periods showing a sustained theta band (>30 s) and negligible movement.

Sharp-wave/ripples and related biosensor signals

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To detect SWRs, the wide-band electrochemical or electrophysiological signal was band-pass filtered (120–200 Hz), squared and smoothed with a 4.2 ms standard deviation and 42 ms wide Gaussian kernel. The square root of this trace was then used as the power envelope to detect oscillatory bursts. The events exceeding the 98th percentile of the power envelope distribution, having at least five cycles and lasting less than 200 ms were detected as ripples. For the analysis of correlations between integrated ripple power and SWR-triggered sensor signals, a ripple power envelope was obtained upon Hilbert-transforming the band-pass filtered electrochemical signal (ripple band, 120–200 Hz). Different ripple integration times were obtained by smoothing the power envelope with moving average windows of different lengths. Correlations were then computed between smoothed ripple power envelopes at SWR times and the corresponding changes in COA or O2 (relative to their baseline value 1 s prior to SWRs) at different lags from SWRs.

The amplitudes of SWR-related COA and O2 were obtained from the difference between the values at SWR lags corresponding to peaks and onsets, extracted from average SWR-triggered traces.

Locomotion bouts

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To detect locomotion bouts in freely moving recordings, speed was computed from the derivative of low-pass filtered position (0.5 Hz). Speed was then band-pass filtered (0.02–0.2 Hz) and locomotion bouts were detected as peaks in speed that exceeded a manually set threshold. In head-fixed recordings on the disc, mouse locomotion was derived from its rotation in 1 s bins. When head-fixed on the treadmill, mouse locomotion was quantified based on the optic flow from a recorded video, choosing a region of interest that covered only a moving part of the treadmill. The signal was resampled to 1 Hz in order to match the sampling rate of locomotion on the rotating disc. Locomotion bouts were detected as peaks on the band-pass filtered speed (0.02–0.2 Hz) that exceeded a manually defined threshold.

The amplitude of locomotion-bout-associated COA and O2 signal transients was calculated based on the difference between the values at manually determined times of transient onsets and peaks, associated with each event. Likewise, the times of locomotion bout onsets (used to calculate speed change) and the associated peaks in theta power were manually defined based on visual inspection of speed time courses and LFP spectrograms, respectively.

Oxygen-related ChOx transient signals analysis

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The amplitude of broad-band spontaneous and exogenously induced O2 transients and COA transients associated with them was calculated, for each event, as the difference between the peak value and that at semi-automatically defined time of the transient’s onset. For the detection of spontaneous O2 transients occurring outside periods with SWRs and locomotion, the O2 peaks occurring within −5 s to +1 s from SWRs or within −14 s to + 4 s from peaks in speed were excluded.

To capture and separately analyze spontaneous COA and O2 transients of highly variable timescale (1–14 s) and non-harmonic shape we performed peak detection on the two signals band-pass filtered in a frequency range [f/2 f] with corner frequency f, defining each filter, varying from 0.05 to 0.5 Hz. Peaks of the transients were then detected in both band-passed COA and O2 signals, selecting the events that exceeded half of the maximal peak amplitude. In each frequency band, COA transients used for the analysis were restricted to be within 0.75/f of any O2 peak. The amplitudes of O2 and COA transients were defined as peak magnitudes derived from the band-pass filtered signals. For each filter band, the linear relationship between COA and O2 peak amplitudes was estimated as a slope of the linear model fit to all detected event pairs for this filter. To interpret the nonlinear shape of the O2 transients captured by each filter, we extracted rise time (from trough to peak) from the median peak aligned O2 transients and used these values in Figure 7E–H and Figure 7—figure supplement 1.

The transient onsets and peaks of exogenously induced changes in O2 and COA were manually detected.

Modeling biosensor responses in vitro

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We simulated biosensor responses in vitro by numerically solving a system of partial differential equations that describe the diffusion of the substrates Ch and O2 in the enzyme coating and their interaction with the enzyme, leading to product formation.

The buffer solution where the biosensor was placed for calibration is a free-flow environment, in which the concentrations of Ch and O2 are constant over time. Therefore, considering R is the coating thickness, at the boundary between the biosensor coating and the calibration buffer Ch is kept constant at 5 μM during the calibration.

(5) Ch(R,t)=5andCht|R=0

Oxygen is changed in steps (x), starting from 0, during the calibration, so between O2 step increases we have:

(6) O2(R,t)=xandO2t|R=0

Enzyme substrates (Ch and O2) diffuse from the bulk solution into the enzyme layer, eventually reaching the electrode site, whereas H2O2 is locally generated and diffuses within the coating. As the size of our recording sites is very small, this process is better described by a spherical diffusion equation:

(7) Difs=[S]t=Ds1r2r(r2[S]r)

where S represents substrates or H2O2 concentration, DS is the respective diffusion coefficient, and r is the distance to the electrode surface.

In order to simulate realistic two-substrate biosensor responses, we modeled the formation of enzyme intermediate complexes resulting from Ch binding to the enzyme and O2 oxidation reactions, which have been described in detail (Fan and Gadda, 2005; Figure 4—figure supplement 2). Briefly, enzyme-bound Ch (ECh), which is in equilibrium with the free reactant species (E and Ch), undergoes a chemical step leading to the reduction of the FAD enzyme prosthetic group. In this nearly irreversible step, Ch is converted to betaine aldehyde, which remains mostly enzyme-bound (EredBA). The first step in which H2O2 is produced results from the oxidation of FADred by O2 (EoxBA) followed by a second chemical step in which FADox is reduced by betaine aldehyde. The resulting enzyme-bound glycine betaine (EredGB) is then oxidized by O2, producing H2O2. The reaction cycle is completed with release of glycine betaine bound to FAD-oxidized enzyme (EoxGB).

The instantaneous change in the concentration of enzyme substrates, free enzyme, enzyme-bound intermediate complexes and reaction products can then be described by the following system of partial differential equations:

(8) Et=kf[E][Ch]+kr[ECh]+k5[EoxGB][Ch]t=DifChkf[E][Ch]+kr[ECh][ECh]t=kf[E][Ch]kr[ECh]k1[ECh][O2]t=DifO2k2[EredBA][O2]k4[EredGB][O2][EredBA]t=k1[ECh]k2[EredBA][O2][EoxBA]t=k2[EredBA][O2]k3[EoxBA][EredGB]t=k3[EoxBA]k4[EredGB][O2][EoxGB]t=k5[EoxGB]+k4[EredGB][O2][H2O2]t=DifH202+k2[EredBA][O2]+k4[EredGB][O2]

Note that we ignored the kinetics of glycine betaine formation, as it is not relevant regarding the sensor signal transduction and it would not affect the concentrations of any reaction species.

In the beginning of the simulated calibration, there is no Ch in the coating, so all enzyme molecules are free, at a concentration that equals the total enzyme concentration ([E]0).

(9) [E](r,t0)=[E]0r=r1,...,R.

In turn, in the same conditions, there are no enzyme-bound species and no product formed.

(10) [ECh](r,t0)=[EredBA](r,t0)=[EoxBA](r,t0)=[EredGB](r,t0)=[EoxGB](r,t0)=[H2O2](r,t0)=0,r=r1,...,R.

At the electrode surface, there is zero flux of substrates and H2O2 is rapidly oxidized, whereas at the boundary between the coating and bulk solution it is rapidly washed away.

(11) Cht|r1=0,O2t|r1=0, [H2O2](r1)=0and[H2O2](R)=0.

The sensor response current is then given by the charge of two-electrons per each oxidized H2O2 molecule times the flux of H2O2 at the electrode surface.

(12) I=2F(πre2)JH2O2,JH2O2=DH2O2H2O2r|r1,

where F is the Faraday’s constant and πre2 is the electrode surface area.

Considering the initial conditions described above the system of partial differential equations was numerically solved by discretization in space and time (time and space steps dt = 0.1 ms and dr = 1 μm, respectively) using the finite difference approximation method (Baronas et al., 2009).

The values ascribed to the variables used in the model are summarized in Table 2. The rate constants corresponding to the reaction mechanism in Figure 4—figure supplement 2 were extracted from the corresponding literature (Fan and Gadda, 2005). An enzyme concentration in the coating of 263 uM was estimated from its concentration in the mixture used for coatings, ignoring drying effects upon coating. Variations in enzyme concentration were simulated around that value. We estimated the diffusion coefficients of enzyme substrates and H2O2 in the coating taking into account the free diffusion coefficients in solution multiplied by a hindrance factor α. The latter was set at 0.8, considering the expected effect of macromolecular crowding on diffusion, for protein concentrations in the range of those used in our simulations (Lamers-Lemmers et al., 2000; Santos et al., 2011).

Table 2
Values of constants used in the biosensor model.
ConstantValueReference
kf*2 × 106 M−1 s−1Fan and Gadda, 2005
kr*580 s−1Fan and Gadda, 2005
k193 s−1Fan and Gadda, 2005
k28.64 × 104 M−1 s−1Fan and Gadda, 2005
k3135 s−1Fan and Gadda, 2005
k45.34 × 104 M−1 s−1Fan and Gadda, 2005
k5200 s−1Fan and Gadda, 2005
DCh1197 μm2 s−1Valencia and González, 2012
DO22500 μm2 s−1Santos et al., 2011
DH2O21830 μm2 s−1van Stroe-Biezen et al., 1993
α0.8
  1. * Estimated based on Kd, Kcat and Km values of ChOx for choline.

    Estimated using the Stokes−Einstein Gierer-Wirtz Estimation method.

  2. Extrapolated from room temperature to 37°C using a factor of 2.6% per degree (Han and Bartels, 1996).

Statistical analysis

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All statistical tests were performed using Matlab. Prior to statistical comparisons, the normality of the data was tested by a Anderson-Darling test. In the case of non-normal distributions, comparisons between two groups were performed using a non-parametric two-sided sign test (signtest, Matlab). To test the effect of two factors on non-normal data (effect of ripple count and power on COA or O2 transients), an aligned-rank-transformation (Wobbrock et al., 2011) was applied followed by two-way ANOVA for unbalanced data (nanova, Matlab). Whenever normality could not be discarded, two-sided two-sample t-tests (paired or unpaired) were used to compare two groups. Two-sided one-sample t-tests were used to test deviations from the null hypothesis (zero). Multiple comparisons were done by one-way ANOVA for unbalanced data. The effect of two or more factors was accounted for by two- or three-way ANOVA for unbalanced data.

Data were presented as average ±95% confidence interval (CI) to allow easy assessment of the significance of estimates. Average corresponds to the mean in normal data or to the median otherwise. The CI of medians was computed based on fractional order statistics (Hutson, 1999). Confidence intervals for correlation coefficients and COA vs. O2 slopes were computed from the percentiles 2.5 and 97.5 of bootstrapped data.

Data availability

Source data and code for figures 1,2, 5-8 as well as raw and intermediate data for the analysis in Figure 3 are provided. The code used to model biosensor responses and obtain the plots in Figure 4 is also available. All data are deposited at https://doi.org/10.5281/zenodo.4564638.

The following data sets were generated
    1. Sirota A
    2. Santos RM
    (2021) Zenodo
    Phasic oxygen dynamics confounds fast choline-sensitive biosensor signals in the brain of behaving rodents.
    https://doi.org/10.5281/zenodo.4564638

References

  1. Book
    1. Baronas R
    2. Ivanauskas F
    3. Kulys J
    (2009)
    Mathematical Modeling of Biosensors: An Introduction for Chemists and Mathematicians
    Springer Series on Chemical Sensors and Biosensors.
  2. Conference
    1. Wobbrock JO
    2. Findlater L
    3. Gergle D
    4. Higgins JJ
    (2011)
    The aligned rank transform for nonparametric factorial analyses using only anova procedures
    Proceedings of the 2011 Annual Conference on Human Factors in Computing Systems.

Decision letter

  1. Tianyi Mao
    Reviewing Editor; Oregon Health and Science University, United States
  2. Joshua I Gold
    Senior Editor; University of Pennsylvania, United States
  3. George Wilson
    Reviewer
  4. Shih-Chieh Lin
    Reviewer

In the interests of transparency, eLife publishes the most substantive revision requests and the accompanying author responses.

Acceptance summary:

As one of the major neuromodulators, acetylcholine signaling plays vital roles in shaping cortical functions. Therefore, faithfully monitoring and measuring acetylcholine dynamics in vivo under behavioral relevant context is important for our understanding of cholinergic modulation. In this study, Santos and Sirota built upon previously used choline oxidase-based electrochemical biosensors and developed a novel acetylcholine sensor with much improved properties, including simultaneous measurement of choline oxidase activity and oxygen levels within the same tetrode. Using the new sensor in freely-moving and head-fixed rodents, the authors observed strong correlation between choline oxidase and oxygen in vivo. The authors then obtained experimental evidence both in vitro and in vivo in combination with mathematical modeling to suggest that measurement using electrochemical biosensors based on choline oxidase is confounded by oxygen dynamics itself. These findings challenge the belief that choline oxidase-based sensors can measure sub-second temporal dynamics of choline concentrations in vivo, and also calls for critical re-evaluation of all oxidase-based biosensors literature to determine the contribution of phasic oxygen dynamics in vivo.

Decision letter after peer review:

Thank you for submitting your article "Phasic oxygen dynamics underlies fast choline-sensitive biosensor signals in the brain of behaving rodents" for consideration by eLife. Your article has been reviewed by Joshua Gold as the Senior Editor, a Reviewing Editor, and three reviewers. The following individuals involved in review of your submission have agreed to reveal their identity: George Wilson (Reviewer #2); Shih-Chieh Lin (Reviewer #3).

The reviewers have discussed the reviews with one another and the Reviewing Editor has drafted this decision to help you prepare a revised submission.

We would like to draw your attention to changes in our revision policy that we have made in response to COVID-19 (https://elifesciences.org/articles/57162). Specifically, when editors judge that a submitted work as a whole belongs in eLife but that some conclusions require a modest amount of additional new data, as they do with your paper, we are asking that the manuscript be revised to either limit claims to those supported by data in hand, or to explicitly state that the relevant conclusions require additional supporting data.

Our expectation is that the authors will eventually carry out the additional experiments and report on how they affect the relevant conclusions either in a preprint on bioRxiv or medRxiv, or if appropriate, as a Research Advance in eLife, either of which would be linked to the original paper.

Summary:

In the manuscript, the authors developed a novel tetrode-based amperometric choline oxidase (ChOx) sensor that can simultaneously measure ChOx and O2 levels within the same tetrode. This sensor allowed the authors to observe strong correlations between ChOx and O2 levels in vivo in behaving rats and mice and under several distinct behavioral contexts. The authors further combined in vivo as well as in vitro perturbation experiments to demonstrate that phasic fluctuations in O2 concentration can lead to fluctuations in ChOx measurements. These findings also challenge the long-held belief that ChOx sensors can measure sub-second temporal dynamics of choline concentrations in vivo, and also calls for critical re-evaluation of all oxidase-based biosensors literature to determine the contribution of phasic O2 dynamics in vivo.

Essential revisions:

The following two main concerns need to be addressed:

1) Further characterization and clarification are needed regarding the sensor properties. This is crucial for the potential users in the field to judge and use the sensor, and for interpretation of the biology results using the sensor.

i) Clear statement in prominent places about the improvement of the sensor and new potential for its biologic applications separating from the authors' 2015 paper.

ii) Regarding the choline responses: characterizing the linearity of choline response is important for users to understand the sensor properties. Related, demonstration how to calibrate moving artificial signals in freely-moving rodents will be useful for the future applications. Further, since the COA signal is confounded by phasic O2 fluctuations, the authentic changes in COA are potentially interfered by O2-evoked enzymatic responses. The interpretation of the signal interference needs to be clearly discussed, including O2-evoked changes, and other related signaling changes, like DA.

iii) The dimensions of the sensor head need to be specified and spelled out clearly. It seems to be around 50 um, but the text seems to suggest 150 um. The individual sensing elements are 17 μm in diameter. If this is true, it is very exciting because it exhibits hemispherical diffusion yielding higher response and enhanced sensitivity. This may improve spatial and temporal resolution if this is in indeed a much smaller sensor as a disk-shaped one.

iv) The role of the sentinels with differential plating is very interesting, but the function of the sentinels is not clear (Introduction "canceling LFP-related currents"). They consume oxygen. Why does this not result in overlap of the diffusion layer for the choline sensor and therefore affect choline response? Please explain why differential electroplating was employed.

v) Please explain how time-dependent behavior of the sensor was measured. This process typically leads to the formation of a film on this electrode surface which can affect sensitivity. According to the authors' 2015 paper, the method for measuring the response time seems rather crude, and may overestimate the response time which is related to the mixing of the solution. This needs to be discussed.

vi) The effect of LFP and other perturbations of sensor responses need to be more clearly explained.

2) Re-organization of the manuscript to improve the readability. This manuscript contains the characterization of the TACO sensor and application of this sensor to monitor real-time behavior in freely moving rodents. The design and characterization of the sensor is intermingled with the application of studying the choline biology with the sensor, making the logic flow hard to follow. The arrangement and presentation of the figures need to be improved so readers can appreciate both characterization and applications aspects and how they are tightly linked. This might also involves properly arrange main figures and associated supplementary figures.

Reviewer #1:

Santos and Sirota developed a novel Tetrode-based Amperometric ChOx (TACO) sensor. This multichannel configuration can simultaneously measure the ChOX activity (COA) and O2 in the same brain spot. Using the TACO sensor in freely-moving and head-fixed rodents, they found that COA and O2 dynamics following locomotion in active state and hippocampal sharp-wave/ripple (SWR) complexes during quiescence state. It's interesting that the COA signal can be calibrated by subtraction of the pseudo-sentinel from the Ch-sensing sites signal the TACO sensor. However, the COA signal is confounded by phasic O2 fluctuations, so, the authentic changes in COA are interfered by O2-evoked enzymatic responses. This question isn't addressed in this paper.

1) The author found that the COA readout is confounded by phasic O2 fluctuations in in vitro and in vivo experiments. These results cast doubt on the validity of the authentic cholinergic response in freely-moving or head-fixed rodents. These findings seem to be generalized to other oxidase-based biosensors, although the author has some discussion on how to address this question. However, we can't get authentic cholinergic dynamic in vivo by TACO biosensor if we didn't clear the biosensor O2 dependence. So, the author should try to address this question.

2) The author should demonstrate that how to calibrate moving artificial signals in freely-moving rodents.

3) Concerns on the selectivity. Figure 1E shows the TACO sensor also responses to dopamine and ascorbate. The author should demonstrate the selectivity of TACO sensor on different monoamines at different concentrations.

Reviewer #2:

This is an important piece of work addressing in-vivo measurements where two coupled components are to be measured ideally in the same time and space components. Unfortunately, the impact of this work is likely to be minimized due to its poor organization and the attempt to deal with a number of separate but related issues in the same manuscript. Accordingly, it is suggested that this work be divided into two manuscripts to be published together. The focus of the two might be:

A) A Tetrode-Based Microsensor (TACO) – This work would focus on the criteria for performance that would be expected for a new sensor, presumably with new and unique properties. This work would include differential plating of electrodes (It is unclear whether some or all of this work has been previously reported), dimension considerations, and the simulation of sensor response. (An important consideration but frequently overlooked is that a sensing element with a 17um diameter will exhibit hemispherical diffusion (Equation 4)). Other issues such as interferences, stability, sensor response time and linearity need to be more fully explained. Presumably such a sensor configuration would be useful in other applications involving oxidase-based sensors.

B) Effects of Local Field Potential and Oxygen-evoked ChOx transients in the In-Vivo Measurement of Acetylcholine in Freely Moving Rodents (A better title can surely be presented!) – Here the focus should be on the in-vivo measurements including a qualitative explanation of the LFP and O2 response and how the TACO sensor corrects for this. Presumably the detection of REM and NREM sleep will be detected by EEG. This is not well explained. Also unclear is how the improved performance of the sensor affects the conclusions of the in-vivo studies.

Reviewer #3:

In this manuscript, Santos and Sirota demonstrated that the in vivo fast choline dynamics detected using choline-oxidase based biosensors is strongly correlated with, and likely caused by, phasic oxygen dynamics in vivo. The authors developed a novel tetrode-based amperometric choline oxidase (ChOx) sensor that can simultaneously measure ChOx and O2 levels within the same tetrode, which enabled the authors to observe strong correlations between ChOx and O2 levels in vivo (in behaving rats and mice, and under several distinct behavioral contexts). To dissect the causal relationship and determine the role of phasic O2 transients, the authors further combined in vivo as well as in vitro perturbation experiments to demonstrate that phasic fluctuations in O2 concentration can lead to fluctuations in ChOx measurements. Moreover, mathematical modeling recapitulates the systemic relationship between ChOx and O2, suggesting the source of this coupling stems from non-steady-state enzyme kinetics. Together, these findings challenge the long-held belief that ChOx sensors can measure sub-second temporal dynamics of choline concentrations in vivo, and also calls for critical re-evaluation of all oxidase-based biosensors literature to determine the contribution of phasic O2 dynamics in vivo.

The study provides extensive evidence to support their claim: correlational, causal, analytical and modeling. The authors employed multiple levels of approaches, from the development of novel biosensors that leads to the observed correlation, to careful in vivo and in vitro perturbation experiments to demonstrate causal relationship. The data is carefully analyzed, and elegantly matched with modeling results. The results of this study have broad implications beyond the ChOx literature and in fact challenge the entire literature on oxidase-based biosensors. I recommend the publication of this paper in eLife as it stands.

https://doi.org/10.7554/eLife.61940.sa1

Author response

Essential revisions:

The following two main concerns need to be addressed:

1) Further characterization and clarification are needed regarding the sensor properties. This is crucial for the potential users in the field to judge and use the sensor, and for interpretation of the biology results using the sensor.

We are grateful to the reviewers and editors to raise such important questions regarding the characterization of sensor properties. The feedback surely contributes to clarify important aspects of the sensor.

i) Clear statement in prominent places about the improvement of the sensor and new potential for its biologic applications separating from the authors' 2015 paper.

Previous enzyme-based biosensor designs, including the ChOx biosensor described in our publication on 2015 (Santos et al., 2015), were based on the differential coating of electrode sites with matrices containing or lacking ChOx. This modification render the sites Chsensitive or insensitive, respectively. The latter have been termed “sentinel” sites, as they are designed to respond to any perturbation except to the analyte of interest (Ch in this case). By subtracting the sentinel from the Ch-measuring site, this approach has been useful to decrease the contribution of interferent signals, namely caused by electrochemical oxidation of electroactive compounds or by voltage fluctuations associated with LFP. However, crosstalk caused by H2O2 diffusion from enzyme-coated to sentinel sites poses important constraints on this design. The inter-site spacing required to avoid diffusional cross-talk leads, for example, to uncontrolled differences in the amplitude and phase of LFP across sites, compromising common-mode rejection.

In the current study, we have circumvented diffusional cross-talk-related limitations by implementing a novel sensing approach. Rather than changing the coating composition across recording sites, we have differentially modified their electrocatalytic properties towards H2O2, resulting in Ch-sensitive and pseudo-sentinel sites. As Ch responses depended solely on the intrinsic properties of the metal surface, we could dramatically reduce the size and increase the spatial density of recording sites by using tetrode configuration. Tetrodes, a bundle of four twisted wires glued together, are conventionally used for separating single neuron action potentials based on the spatial structure of their action potentials across wires. Here, the spatial structure of the electrochemical signal is created by electrochemical modification of wires. Importantly this design allows the unbiased measurement of ChOx activity and O2 in the same brain spot by using a tetrode site to directly measure the latter. This has not been possible to achieve with conventional enzyme-based biosensor designs, including our own previous stereotrode design.

We acknowledge that the improvements of the TACO sensor over our previous stereotrode design, published in 2015 (as well as other conventional enzyme-based biosensors in general), were not clearly emphasized in the manuscript. We added new paragraphs/sentences in the Introduction and Results of the revised manuscript highlighting the main difference between the two sensors and advantages of the new design for the unbiased measurement of the signals derived from ChOx activity (COA) and O2.

ii) Regarding the choline responses: characterizing the linearity of choline response is important for users to understand the sensor properties.

Responses to choline were highly linear within the concentration range tested (up to 30 µM). This information was added to Table 1 and mentioned in the text (Results) of the revised manuscript.

Related, demonstration how to calibrate moving artificial signals in freely-moving rodents will be useful for the future applications.

Movement can cause electromagnetic or mechanical perturbations (movement artifacts) that are expected to scale with the impedance of individual recording sites. As the same applies for LFP-related currents, it is not trivial to discriminate both confounds. Nevertheless, our common-mode rejection approach, which is optimized by a frequency-domain correction of electrode impedances (please check Materials and methods section, for detailed explanation), is designed to optimally remove both LFP- and movement-related artifacts.

In our freely-moving recordings we did not have prominent movement-related perturbations, probably due to the proximity of the head-stage to the sensor and the shielding effect of the grounded copper mesh that covers the implant. Nevertheless, candidate events likely caused by movement consisted in current deflections aligned to locomotion bouts, which were completely removed by common-mode rejection. In the revised manuscript we added the average raw traces triggered on locomotion bouts in Figure 2D, highlighting the usefulness of our method to remove putative movement-related artifacts in addition to LFP and other interferents. We have also added a brief mention to this issue in the Results.

Further, since the COA signal is confounded by phasic O2 fluctuations, the authentic changes in COA are potentially interfered by O2-evoked enzymatic responses. The interpretation of the signal interference needs to be clearly discussed, including O2-evoked changes, and other related signaling changes, like DA.

The main focus of our study was to investigate the effect of physiological O2 fluctuations on the ChOx biosensor signal, which is given by the activity of immobilized ChOx, which we abbreviate as COA across the manuscript. In order to address this issue in an unbiased manner it is essential to clean artifacts that directly generate currents on the electrode surface (please see response to point 1vi for details). Our TACO sensor was designed to optimize the removal of such confounds, resulting in a clean COA signal. As this signal reflects the activity of immobilized enzyme, it is sensitive to changes in O2, not only Choline. Thus, the COA signal is not confounded, but rather modulated by changes in O2. Our main finding was that phasic O2 modulation of COA is a major confound of phasic Ch dynamics measurements using ChOx sensors in vivo in the brain. In this sense, the central tenet of the paper is that COA is not reflecting an authentic choline concentration dynamics, but rather a nonlinear function of Ch and O2 dynamics, with no feasible analytical approach to separate the two. We recognize that, in the Materials and methods section, the description of how the COA signal was computed could lead to confusion between authentic COA and authentic Ch measurement. In the revised manuscript we have changed the terms used in the signal cleaning procedure.

Regarding neurochemical confounds (e. g. ascorbate or dopamine and other monoamines), we acknowledge that the description of multichannel sensor properties in Table 1 could be confusing to readers. The table was also not conveying the important information on how sensitive is our COA measurement to these artifacts. In the revised manuscript we have removed the information about selectivity ratios for individual sites. Instead, the table section now called “Analytical properties for COA measurement” was expanded and now shows DA and AA sensitivities and selectivity ratios for the COA signal, computed from the difference between Au/Pt/m-PD and Au/m-PD sites.

Additionally, we added a column in the color plot in Figure 1E describing the relative responses of the COA measurement to the different factors. This addition highlights the high selectivity of the COA signal for Ch, as compared with individual sites.

Finally, we have detailed the interpretation of the freely-moving signals triggered on SWRs and locomotion bouts. In the Materials and methods section of the revised manuscript, we clarify how the differential signals COAnon-mPD and NCC (neurochemical confounds) presented in Figure 2 (revised version) were computed. In the description of these results, we also explain how the response patterns of raw and cleaned signals can be used to infer the contribution of different sorts of artifacts, including movement- and LFP-related and those caused by neurochemicals (Results).

iii) The dimensions of the sensor head need to be specified and spelled out clearly. It seems to be around 50 um, but the text seems to suggest 150 um. The individual sensing elements are 17 μm in diameter. If this is true, it is very exciting because it exhibits hemispherical diffusion yielding higher response and enhanced sensitivity. This may improve spatial and temporal resolution if this is in indeed a much smaller sensor as a disk-shaped one.

We thank the reviewers for referring to this point. It is an important detail that was not clearly stated in the manuscript. In the Materials and methods section, the description of the insertion of the tetrode inside a silica tube might have been misleading. In fact, the tetrode actually protrudes 1-2 cm out of the silica tube. This distance assures that the latter is not in contact with the brain in in vivo recordings. The cutting of the twisted ending of the tetrode results in four disc-shaped sensing elements with 17 µm diameter. The total diameter of the tetrode is approximately 60 µm. In the revised manuscript we have clarified and emphasized these details in the Materials and methods section, in the Results and with an additional cartoon in Figure 1A.

iv) The role of the sentinels with differential plating is very interesting, but the function of the sentinels is not clear (Introduction "canceling LFP-related currents"). They consume oxygen. Why does this not result in overlap of the diffusion layer for the choline sensor and therefore affect choline response? Please explain why differential electroplating was employed.

We further clarified the role of the pseudo-sentinel sites on the removal of LFP-related currents and neurochemical artifacts and expanded the reasoning behind this approach. Please check the Introduction of the revised manuscript.

When polarized at +0.6 V vs. Ag/AgCl, the pseudo-sentinel channels display a residual activity towards electrochemical oxidation of H2O2. This electrochemical reaction generates O2, but the effect on the local O2 concentration is negligible due to the poor sensitivity and very small electrode surface area (17 µm diameter disc). We measured O2 (head-fixed mice and in vitro) by electrochemical reduction at -0.2 V vs. Ag/AgCl at a pseudo-sentinel site (gold-plated without m-PD). In this case O2 is consumed, but at a very limited extent that does not affect the local O2 level in the sensor. In accordance with the expected lack of effect on O2 levels, we have confirmed that switching the applied potential on a gold-plated site between +0.6 V and -0.2 V vs. Ag/AgCl has no effect on the COA signal. In the revised manuscript we added a supplementary figure (Figure 3—figure supplement 1) describing this observation. Accordingly, we extended the discussion of this topic in the Results section.

v) Please explain how time-dependent behavior of the sensor was measured. This process typically leads to the formation of a film on this electrode surface which can affect sensitivity. According to the authors' 2015 paper, the method for measuring the response time seems rather crude, and may overestimate the response time which is related to the mixing of the solution. This needs to be discussed.

The sensor response times were estimated from the rise of the current in response to analyte additions in a stirred buffer solution, as described in the Materials and methods section. In the revised manuscript, we added a sentence to further clarify the use of this setup to estimate response times. Indeed, this setup is not the most appropriate to precisely determine response times due to the bias introduced by the analyte mixing time after its addition to the buffer. Our previous study (Santos et al., 2015) suggests however that the biggest contribution to the estimated response time is due to diffusion of Ch in the sensor coating. Besides the fact that we cannot precisely determine response times, it is noteworthy that real response times are faster than the values we report. This further highlights the high temporal resolution of the TACO sensor. We added a paragraph discussing this topic in the revised manuscript (Results).

vi) The effect of LFP and other perturbations of sensor responses need to be more clearly explained.

Two main types of artifacts affect the response of enzyme-based electrochemical biosensors: electromagnetic or electrochemical sources that directly generate currents at the electrode surface and biochemical factors that affect the activity of the immobilized enzyme. The first group can be sub-divided into: (a) artifacts that generate faradaic currents, arising from oxidation/reduction of electrochemically active molecules, such as ascorbate or dopamine; (b) artifacts that change the charge distributions at the electrode surface, generating capacitive currents, which in the brain are mainly caused by local fluctuations in field potentials (LFP) generated by the trans-membrane current sources of the surrounding neural tissue. Effectively, LFP causes potential changes at the electrode surface who’s voltage is clamped by the potentiostat circuit, giving rise to apparent current, similar to voltage clamp measurement of the intracellular current. The second group, consisting in biochemical artifacts, comprises mainly the effect of oxygen on enzymatic activity (although other factors such as temperature and pH might have a minor effect, as discussed in the manuscript, Discussion).

Importantly, the strategies devised to reduce artifacts that directly generate electrochemical currents (chemical surface modifications or common-mode rejection) do not control for factors influencing immobilized ChOx activity.

Since O2 interference was the main focus of the paper and is thoroughly described throughout the manuscript, in the Introduction of revised manuscript we extended the description of the factors directly generating currents on the electrode surface (Introduction).

2) Re-organization of the manuscript to improve the readability. This manuscript contains the characterization of the TACO sensor and application of this sensor to monitor real-time behavior in freely moving rodents. The design and characterization of the sensor is intermingled with the application of studying the choline biology with the sensor, making the logic flow hard to follow. The arrangement and presentation of the figures need to be improved so readers can appreciate both characterization and applications aspects and how they are tightly linked. This might also involves properly arrange main figures and associated supplementary figures.

We believe this suggestion stems from the expectation that we may have conveyed to the readers regarding the possibility of measuring authentic Ch dynamics in behaving animals with our TACO sensor. Indeed, the TACO sensor design makes it ideally suited for the unbiased measurement of brain Ch dynamics based on ChOx, while controlling for O2 changes that might modulate immobilized enzyme activity. However, our data shows that phasic ChOx activity (COA) is dominated by O2 fluctuations in the brain of behaving animals. The complexity of the nonlinear interplay between COA and O2, which depends on multiple time-scale concentration dynamics of both enzyme substrates made it impossible to extract authentic Ch from the in vivo COA signal.

Following the logic of data presentation in our manuscript, the initial description of TACO sensor design and properties towards COA measurement was followed by its in vivo application in freely-moving and head-fixed rodents, which led to the discovery of the possible O2 confound. This, in turn, prompted the next in vivo experiments with causal manipulations to prove the hypothetical confound effect. Next, in vitro experiments were used for more systematic investigation of the details of the confound and its underlying causes guided by the prior in vivo observations. Finally, we used a detailed mathematical model to quantitatively uncover the mechanism of the oxygen confound of the choline-oxidase-based biosensor.

We think this logic of exposition is guiding the reader through our thought process and progresses consistently from the development of novel methodology to evaluation and identification of the confound, and then to unraveling the mechanism in vivo, in vitro and in the model. Reversing the order of presentation would break this logic and hurt the presentation of the story.

We would like to ask the editor for her consent not to follow the suggested major reorganization. Instead, we clarified the internal logic at the end of the Introduction, as well as throughout exposition of the results. Moreover, throughout the revised manuscript we emphasize the focus of our study on phasic COA dynamics instead of putative Ch by replacing terms alluding to the latter by “COA”. Accordingly, we better articulated the motivation for assessing SWR- and locomotion-related signals in freely moving animals (Figure 2) and the interpretation of these results to avoid a biased expectation of the reader that COA signals provide authentic Ch readout. The revised manuscript now provides an unbiased perspective on motivation and interpretation of the in vivo experiments (Results). The bias of COA by O2 and the issues associated with derivation of authentic Ch dynamics from our measurements were also further explained in the Discussion. Along the same lines, we have trimmed Figure 2 in order to keep the focus of the paper on phasic dynamics of the COA signal. Namely, we moved panels B and C describing tonic COA dynamics in the original manuscript to a supplementary figure in the revised version (Figure 2—figure supplement 2).

https://doi.org/10.7554/eLife.61940.sa2

Article and author information

Author details

  1. Ricardo M Santos

    Bernstein Center for Computational Neuroscience, Faculty of Medicine, Ludwig-Maximilians Universität München, Planegg-Martinsried, Germany
    Contribution
    Conceptualization, Data curation, Software, Formal analysis, Investigation, Visualization, Methodology, Writing - original draft, Writing - review and editing
    For correspondence
    santos@bio.lmu.de
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-6182-1708
  2. Anton Sirota

    Bernstein Center for Computational Neuroscience, Faculty of Medicine, Ludwig-Maximilians Universität München, Planegg-Martinsried, Germany
    Contribution
    Conceptualization, Supervision, Funding acquisition, Project administration, Writing - review and editing
    For correspondence
    sirota@bio.lmu.de
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-4700-6587

Funding

Bundesministerium für Bildung und Forschung (01GQ0440)

  • Anton Sirota

Deutsche Forschungsgemeinschaft (Munich Cluster for Systems Neurology (SyNergy EXC 1010))

  • Anton Sirota

European Union Framework Programme for Research and Innovation (FETPROACT program via grant agreement no.723032 (BrainCom))

  • Anton Sirota

Deutsche Forschungsgemeinschaft (Priority Programs 1665 and 1392)

  • Anton Sirota

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Acknowledgements

We thank Francisco Almeida and Kenneth Klau for technical assistance.

Ethics

Animal experimentation: All experimental procedures were established, and have been approved in accordance with the stipulations of the German animal welfare law (Tierschutzgesetz ) (ROB-55.2-2532.Vet_02-16-170).

Senior Editor

  1. Joshua I Gold, University of Pennsylvania, United States

Reviewing Editor

  1. Tianyi Mao, Oregon Health and Science University, United States

Reviewers

  1. George Wilson
  2. Shih-Chieh Lin

Publication history

  1. Received: August 9, 2020
  2. Accepted: February 12, 2021
  3. Accepted Manuscript published: February 15, 2021 (version 1)
  4. Version of Record published: March 4, 2021 (version 2)

Copyright

© 2021, Santos and Sirota

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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