Understanding and predicting evolutionary dynamics is an ongoing challenge across all fields of biology. Microbial populations offer relatively simple model systems for investigating adaptation on multiple length scales, from the molecular to the population level, on timescales ranging from a few generations to decades. Yet even these simplest of systems exhibit rich and often counterintuitive evolutionary dynamics. Despite enormous progress, both theoretical and experimental, predicting evolution remains exceedingly difficult, in part because it is challenging to identify the phenotypes, selective gradients, and environmental factors shaping adaptation. In turn, controlling those dynamics – for example, by judicious manipulation of environmental conditions – is often impossible. These challenges represent open theoretical questions but also underlie practical public health threats – exemplified by the rapid rise of antibiotic resistance (Davies and Davies, 2010; Levy and Marshall, 2004) – where evolutionary dynamics are fundamental to the challenge, and perhaps, to the solution.

In recent years, evolution-based strategies for impeding drug resistance have gained significant attention. These approaches have identified a number of different factors that could modulate resistance evolution, including spatial heterogeneity (Zhang et al., 2011; Baym et al., 2016a; Greulich et al., 2012; Hermsen et al., 2012; Moreno-Gamez et al., 2015; Gokhale et al., 2018; De Jong and Wood, 2018; Santos-Lopez et al., 2019); competitive (Read et al., 2011; Hansen et al., 2017; Hansen et al., 2020), cooperative (Meredith et al., 2015b; Artemova et al., 2015; Sorg et al., 2016; Tan et al., 2012; Karslake et al., 2016; Yurtsev et al., 2016; Hallinen et al., 2020), or metabolic (Adamowicz et al., 2018; Adamowicz et al., 2020) interactions between bacterial cells; synergy with the immune system, especially in the context of adaptive treatment (Gjini and Brito, 2016); epistasis between resistance mutations (Trindade et al., 2009; Borrell et al., 2013; Lukačišinová et al., 2020); plasmid dynamics (Lopatkin et al., 2016; Lopatkin et al., 2017; Cooper et al., 2017); precise tuning of drug doses (Lipsitch and Levin, 1997; Yoshida et al., 2017; Meredith et al., 2015a; Nichol et al., 2015; Fuentes-Hernandez et al., 2015; Coates et al., 2018; Iram et al., 2021); cycling or mixing drugs at the hospital level (Bergstrom et al., 2004; Beardmore et al., 2017); and statistical correlations between resistance profiles for different drugs (Imamovic and Sommer, 2013; Kim et al., 2014; Pál et al., 2015; Barbosa et al., 2017; Rodriguez de Evgrafov et al., 2015; Nichol et al., 2019; Podnecky et al., 2018; Imamovic et al., 2018; Barbosa et al., 2019; Rosenkilde et al., 2019; Apjok et al., 2019; Maltas and Wood, 2019; Maltas et al., 2020; Hernando-Amado et al., 2020; Roemhild et al., 2020; Ardell and Kryazhimskiy, 2020).

Drug combinations are a particularly promising approach for slowing resistance (Baym et al., 2016b), but the evolutionary impacts of combination therapy remain difficult to predict, especially in a clinical setting (Podolsky, 2015; Woods and Read, 2015). Antibiotics are said to interact when the combined effect of the drugs is greater than (synergy) or less than (antagonism) expected based on the effects of the drugs alone (Greco et al., 1995). These interactions may be leveraged to improve treatments – for example, by offering enhanced antimicrobial effects at reduced concentrations. But these interactions can also accelerate, reduce, or even reverse the evolution of resistance (Chait et al., 2007; Michel et al., 2008; Hegreness et al., 2008; Pena-Miller et al., 2013; Dean et al., 2020), leading to tradeoffs between short-term inhibitory effects and long-term evolutionary potential (Torella et al., 2010). In addition, resistance to one drug may be associated with modulated resistance to other drugs. This cross-resistance (or collateral sensitivity) between drugs in a combination has also been shown to significantly modulate resistance evolution (Barbosa et al., 2018; Rodriguez de Evgrafov et al., 2015; Munck et al., 2014).

Collateral effects (Pál et al., 2015; Roemhild et al., 2020) and drug interactions (Bollenbach et al., 2009; Chevereau et al., 2015; Lukačišin and Bollenbach, 2019; Chevereau et al., 2015), even in isolation, reflect interactions – between genetic loci, between competing evolutionary trajectories, between chemical stressors – that are often poorly understood at a mechanistic or molecular level. Yet adaptation to a drug combination may often reflect both phenomena, with the pleiotropic effects that couple resistance to individual drugs constraining, or constrained by, the interactions that occur when those drugs are used simultaneously. In addition, the underlying genotype space is high-dimensional and potentially rugged, rendering the genotypic trajectories prohibitively complex (de Visser and Krug, 2014).

In this work, we attempt to navigate these obstacles by modeling evolutionary dynamics on lower-dimensional phenotype spaces that leverage scaling relations between the drug-response surfaces of ancestral and mutant populations. Our approach is inspired by the fact that multiobjective evolutionary optimization may occur on surprisingly low-dimensional phenotypic spaces (Shoval et al., 2012; Hart et al., 2015). To develop a similarly coarse-grained picture of multidrug resistance, we associate selectable resistance traits with changes in effective drug concentrations, formalizing the geometric rescaling assumptions originally pioneered in Chait et al., 2007; Hegreness et al., 2008; Michel et al., 2008 and connecting evolutionary dynamics with a simple measurable property of ancestral populations. We show that evolved resistance to the component drugs – and in turn, the adaptation of growth rate – is governed by a Price equation whose covariance terms encode geometric features of both (1) the two-drug-response surface in ancestral populations (the drug interaction) and (2) the correlations between resistance levels to those drugs (collateral effects). In addition, we show how evolutionary trajectories within this framework reduce to a type of weighted gradient dynamics on the two-drug landscape, with the drug interaction dictating the shape of the underlying landscape and the collateral effects constraining the motion on those landscapes, leading to deviations from a simple gradient descent. We also illustrate two straightforward extensions of the basic framework, allowing us to investigate the effects of both constrained mutational pathways and sequential multidrug treatments. Our results clarify the complex relationship between drug interactions and collateral effects in multidrug environments and illustrate how specific dosage combinations can shift the weighting of these two effects, leading to different selective outcomes even when the available genetic routes to resistance are unchanged.

Our goal is to understand evolutionary dynamics of a cellular population in the presence of two drugs, drug 1 and drug 2. These dynamics reflect a potentially complex interplay between drug interactions and collateral evolutionary tradeoffs, and our aim is to formalize these effects in a simple model. To do so, we assume that the per capita growth rate of the ancestral population is given by a function $G(x,y)$, where $x$ and $y$ are the concentrations of drugs 1 and 2, respectively. We limit our analysis to two-drug combinations, but it could be extended to higher-order drug combinations, though this would require empirical or theoretical estimates for higher-dimensional drug-response surfaces (see, e.g., Wood et al., 2012; Zimmer et al., 2016; Zimmer et al., 2017; Russ and Kishony, 2018; Tekin et al., 2016; Tekin et al., 2017; Tekin et al., 2018). At this stage, we do not specify the functional form of $G(x,y)$, though we assume that this function can be derived from pharmacodynamic or mechanistic considerations (Engelstädter, 2014; Bollenbach et al., 2009; Wood and Cluzel, 2012) or otherwise estimated from experimental data (Greco et al., 1995; Wood et al., 2014). In classical pharmacology models (Loewe, 1953; Greco et al., 1995), the shape of these surfaces – specifically, the convexity of the corresponding contours of constant growth (‘isoboles’) – determines the type of drug interaction, with linear isoboles corresponding to additive drug pairs. In this framework, deviations from linearity indicate synergy (concave up) or antagonism (concave down). While there are multiple conventions for assigning geometric features of the response surface to an interactions type – and there has been considerable debate about the appropriate null model for additive interactions (Greco et al., 1995) – the response surfaces contain complete information about the phenotypic response. The manner in which this response data is mapped to a qualitative interaction type – and therefore used to label the interaction as synergistic, for example – is somewhat subjective, though in what follows we adopt the isobole-based labeling scheme because it is more directly related to the geometry of the response surface than competing models (e.g., Bliss independence; Greco et al., 1995).

Resistance as a continuous trait and rescaling in a simple model

The primary assumption of the model is that the phenotypic response (e.g., growth rate) of drug-resistant mutants, which may be present in the initial population or arise through mutation, corresponds to a simple rescaling of the growth rate function $G(x,y)$ for the ancestral population. As we will see, this scheme provides an explicit link between a cell’s level of antibiotic resistance and its fitness in a given multidrug environment. Specifically, we assume that the growth rate (g_i) of mutant $i$ is given by

(1) ${\displaystyle g_i=G({\alpha }_{i}x,{\beta }_{i}y),}$

where ${\alpha }_i$ and ${\beta }_i$ are rescaling parameters that reflect the physiological effects of mutations on the growth rate. In some cases – for example, resistance due to efflux pumps (Wood and Cluzel, 2012) or drug degrading enzymes (Yurtsev et al., 2013) – this effective concentration change corresponds to a physical change in intracellular drug concentration. More generally, though, this hypothesis assumes that resistant cells growing in external drug concentration $x$ behave similarly to wild-type (drug-sensitive) cells experiencing a reduced effective concentration $\alpha x$. Similar rescaling arguments were originally proposed in Chait et al., 2007, where they were used to predict correlations between the rate of resistance evolution and the type of drug interaction. These arguments have also been used to describe fitness tradeoffs during adaptation (Das et al., 2020) and to account for more general changes in the dose-response curves, though in many cases the original two-parameter rescaling was sufficient to describe the growth surface in mutants (Wood et al., 2014).

When only a single drug is used, this rescaling leads to a simple relationship between the characteristic inhibitory concentrations – for example, the half-maximal inhibitory concentration (IC₅₀) or the minimum inhibitory concentration (MIC) – of the ancestral (sensitive) and mutant (resistant) populations. In what follows, we refer to these reference concentrations as $K_i^j$, where $i$ labels the cell type and, when there is more than one drug, $j$ labels the drug. Conceptually, this means that dose-response curves for both populations have the same basic shape, with resistance (or sensitivity) in the mutant corresponding only to a shape-preserving rescaling of the drug concentration ($D\to D/K_i$; Figure 1A). In the presence of two drugs, the dose-response curves become dose-response surfaces, and rescaling now corresponds to a shape-preserving rescaling of the contours of constant growth. There are now two scaling parameters, one for each drug, and in general they are not equal. For example, in Figure 1B, the mutant shows increased sensitivity to drug 1 ($\alpha \equiv K_{\text{WT}}^1/K_{\text{Mut}}^1>1$) and increased resistance to drug 2 ( $\beta \equiv K_{\text{WT}}^2/K_{\text{Mut}}^2<1$), where superscripts label the drug (1 or 2) and subscripts label the cell type (wild type, WT; mutant, Mut).

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The power of this rescaling approach is that it directly links growth of the mutant populations to measurable properties of the ancestral population (the two-drug-response surface) via traits of the mutants (the rescaling parameters). Each mutant is characterized by a pair of scaling parameters, $({\alpha }_i,{\beta }_i)$, which one might think of as a type of coarse-grained genotype (Figure 1C). When paired with the ancestral growth surface, these traits fully determine the per capita growth rate of the mutant at any external dosage combination $(x,y)$ via Equation 1. While the scaling parameters are intrinsic properties of each mutant, they contribute to the phenotype (growth) in a context-dependent manner, leading to selection dynamics that depend in predictable ways on the external environment (Figure 1).

We assume a finite set of $M$ subpopulations (mutants), (${\displaystyle i=1,\dots M}$), with each subpopulation corresponding to a single pair of scaling parameters. For simplicity, initially we assume each of these mutants is present in the original population at low frequency and neglect mutations that could give rise to new phenotypes, though later we show that it is straightforward to incorporate them into the same framework. We do not specify the mechanisms by which this standing variation is initially generated or maintained; instead, we simply assume that such standing variation exists, and our goal is to predict how selection will act on this variation for different choices of external (true) drug concentrations. As we will see, statistical properties of this variation combine with the local geometry of the response surface to determine the selection dynamics of these traits.

Population dynamics of scaling parameters

The mean resistance trait to drug 1, which in our case is the scaling parameter $\overline{\alpha }(t)\equiv {\sum }_{i=1}^M{\alpha }_i{f}_i(t)$, evolves according to

(2) ${\displaystyle \frac{d\overline{\alpha }}{dt}=\sum _{i=1}^M{\alpha }_i\frac{d{f}_i(t)}{dt},}$

where $f_i(t)$ is the population frequency of mutant $i$ at time $t$ in the population. Assuming that each subpopulation grows exponentially at the per capita growth rate ($d{n}_i/dt=g_i{n}_i$, with n_i the abundance of mutant $i$ and g_i the per capita growth rate given by Equation 1), the frequency $f_i(t)$ changes according to

(3) ${\displaystyle \frac{d{f}_i}{dt}=\frac{d}{dt}\left(\frac{n_i}{\sum _i{n}_i}\right)=f_i(g_i-\overline{g}),}$

where $\overline{g}={\sum }_{i=1}^M{f}_i{g}_i$ is the (time-dependent) mean value of g_i across all $M$ subpopulations (mutants). Combining Equations 2 and 3, we have

(4) ${\displaystyle \frac{d\overline{\alpha }}{dt}={\mathrm{C}\mathrm{o}\mathrm{v}}_{\mathit{x}}(\alpha ,g),}$

where $\mathrm{Cov}{(\alpha ,g)}_{\mathit{𝒙}}\equiv {\sum }_{i=1}^M{\alpha }_i{f}_i(g_i-\overline{g})$ is the covariance between the scaling parameters ${\alpha }_i$ and the corresponding mutant growth rates g_i. The subscript $\mathit{𝒙}$ is a reminder that the growth rates g_i and $\overline{g}$ that appear in the covariance sum depend on the external (true) drug concentration $\mathit{𝒙}\equiv (x,y)$. An identical derivation leads to an analogous equation for the scaling parameter with respect to drug 2, mean susceptibility to drug 2, $\overline{\beta }$, relative to the original population, and the full dynamics are therefore described by

(5) ${\displaystyle \begin{array}{l}{\displaystyle \frac{d\overline{\alpha }}{dt}={\mathrm{C}\mathrm{o}\mathrm{v}}_{\mathit{x}}(\alpha ,g),}\\ \frac{d\overline{\beta }}{dt}={\mathrm{C}\mathrm{o}\mathrm{v}}_{\mathit{x}}(\beta ,g).\end{array}}$

To complete the model described by Equation 5, one must specify the external (true) concentration of each drug ($\mathit{𝒙}$); a finite set of scaling parameter pairs ${\alpha }_i,{\beta }_i$ corresponding to all ‘available’ mutations; and an initial condition $(\overline{\alpha }(0),\overline{\beta }(0))$ for the mean scaling parameters. When combined with the external drug concentrations, the scaling parameters directly determine the effective drug concentrations ($D_1^{\text{eff}},D_2^{\text{eff}}$) experienced by each mutant according to

(6) ${\displaystyle \begin{array}{l}{\displaystyle D_1^{\text{eff}}={\alpha }_{i}x,}\\ D_2^{\text{eff}}={\beta }_{i}y.\end{array}}$

We note that drug concentrations can be above or below the MIC contour, with higher concentrations leading to population collapse ($G<0$) and lower concentrations to population growth ($G>0$) in the ancestral (drug sensitive) cells. However selection dynamics remain the same in both cases as selection depends only on differences in growth rates between different subpopulations.

Equation 5 is an example of the well-known Price equation from evolutionary biology (Price, 1970; Price, 1972; Frank, 1995; Lehtonen et al., 2020), which says that the change in the (population) mean value of a trait is governed by the covariance of traits and fitness. In general, fitness can be difficult to measure and, in some cases, even difficult to define. However, the rescaling assumption of our model replaces fitness with $g$, which can be directly inferred from measurable properties (the two-drug-response surface) in the ancestral population. In what follows, we will sometimes casually refer to Equation 5 as a ‘model,’ but it is important to note that the Price equation is not, in and of itself, a mathematical model in the traditional sense. Instead, it is a simple mathematical statement describing the statistical relationship between variables, which are themselves defined in some underlying model. In this case, the mathematical model consists of a collection of exponentially growing populations whose per capita growth rates are linked by scaling relationships. Equation 5– the Price equation – does not include additional assumptions, mechanistic or otherwise, but merely captures statistical relationships between the model variables. We will see, however, that these relationships provide conceptual insight into the interplay between collateral effects and drug interactions.

Equation 5 encodes deceptively rich dynamics that depend on both the interaction between drugs and the collateral effects of resistance. First, it is important to note that αs and βs vary together in pairs, and the evolution of these two traits is not independent. As a result, constraints on the joint co-occurrence of ${\alpha }_i$ and ${\beta }_i$ among the mutant subpopulations can significantly impact the dynamics. These constraints correspond to correlations between resistance levels to different drugs – that is, to cross-resistance (when pairs of scaling parameters simultaneously increase resistance to both drugs) or to collateral sensitivity (when one scaling parameter leads to increased resistance and the other to increased sensitivity). In addition, $g$ contains information about the dose-response surface and, therefore, about the interaction between drugs. The evolution of the scaling parameters is not determined solely by the drug interaction or by the collateral effects, but instead by both – quantified by the covariance between these rescaled trait values and the ancestral dose-response surface.

As an example, we integrated the model numerically to determine the dynamics of the mean scaling parameters and the mean growth rate for a population exposed to a fixed concentration of two drugs whose growth surface has been fully specified (Figure 2A). The dynamics can be thought of as motion on the two-dimensional response surface; if the initial population is dominated by the ancestral cells, the mean scaling parameters are approximately 1, and the trajectory therefore starts near the point representing the true drug concentration (in this case, $(x_0,y_0)$), where growth is typically small (Figure 2A). Over time, the mean traits evolve, tracing out a trajectory in the space of scaling parameters (Figure 2B). When the external concentration of drug is specified, these dynamics also correspond to a trajectory through the space of effective drug concentrations, which, in turn, can be linked with an average growth rate through the drug-response surface (Figure 2B). The model therefore describes both the dynamics of the scaling parameters, which describe how resistance to each drug changes over time (Figure 2C), and the dynamics of growth rate adaptation in the population as a whole (Figure 2D).

Figure 2

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Selection dynamics depend on drug interaction and collateral effects

This rescaling model indicates that selection is determined by both the drug interaction and the collateral effects, consistent with previous experimental findings. As a simple example, consider the case of a fixed drug interaction but different qualitative types of collateral effects – that is, different statistical relationships between resistance to drug 1 (via α) and resistance to drug 2 (via β). In Figure 3A, we consider cases where resistance is primarily to drug 2 (black), primarily to drug 1 (cyan), strongly positively correlated (cross-resistance, pink), and strongly negatively correlated (collateral sensitivity, green). Using the same drug interaction surface as in Figure 2, we find that a mixture of both drugs leads to significantly different trajectories in the space of scaling parameters (Figure 3B) and, in turn, significantly different rates of growth adaptation. In this example, cross-resistance (pink) leads to rapid increases in resistance to both drugs (rapid decreases in scaling parameters, Figure 3C, D) and the fastest growth adaptation (Figure 3E). By contrast, if resistance is limited primarily to one drug (cyan or black), growth adaptation is slowed (Figure 3E) – intuitively, purely horizontal or purely vertical motion in the space of scaling parameters leads to only a modest change in growth because the underlying response surface is relatively flat in those directions, meaning that the rescaled concentration, in each case, lies near the original contour (Figure 3). As a result of the contour shapes (drug interaction), resistance to both drugs can develop at approximately the same rate (for example), even when collateral structure suggests resistance to one drug will dominate (e.g., cyan case in Figure 3). We note that the dynamics will depend on the (true) external drug concentrations $(x_0,y_0)$, even if the rescaling parameters remain identical, because a given rescaling transformation will lead to different effective drug concentrations, and therefore different growth rates, for different values of $(x_0,y_0)$.

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When both collateral effects and drug interactions vary, the dynamics can be considerably more complex, and the dominant driver of adaptation can be drug interaction, collateral effects, or a combination of both. Previous studies support this picture as adaptation has been observed to be driven primarily by drug interactions (Chait et al., 2007; Michel et al., 2008; Hegreness et al., 2008), primarily by collateral effects (Munck et al., 2014; Barbosa et al., 2018), or by combinations of both (Baym et al., 2016b; Barbosa et al., 2018; Dean et al., 2020). Figure 4 shows schematic examples of growth rate adaptation for different types of collateral effects (rows, ranging from cross-resistance [top] to collateral sensitivity [bottom]) and drug interactions (columns, ranging from synergy [left] to antagonism [right]). The growth adaptation may also depend sensitively on the external environment – that is, on the true external drug concentration (blue, cyan, and red). In the absence of both drug interactions (linear isoboles) and collateral effects (uncorrelated scaling parameters), adaptation is slower when the drugs are used in combination than when they are used alone, consistent with the fact that adaptation to multiple stressors is expected to take longer than adaptation to each stressor alone (Figure 4; middle row, middle column). As in Figure 3, modulating collateral effects with drug interaction fixed can have dramatic impacts on adaptation (Figure 4, columns). On the other hand, modulating the drug interaction in the absence of collateral effects will also significantly impact adaptation, with synergistic interactions leading to accelerated adaptation relative to other types of drug interaction (Figure 4, middle row; compare green curves across row). Similar interaction-driven adaptation has been observed in multiple bacterial species (Chait et al., 2007; Michel et al., 2008; Hegreness et al., 2008; Dean et al., 2020).

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Model predicts experimentally observed adaptation of growth and resistance

Our model makes testable predictions for adaptation dynamics of both the population growth rate and the population-averaged resistance levels to each drug (i.e., the mean scaling parameters) for a given drug-response surface, a given set of available mutants, and a specific combination of (external) drug dosages. To compare predictions of the model with experiment, we solved Equation 5 for the 11 different dosage combinations of tigecycline (TGC) and ciprofloxacin (CIP) used to select drug-resistance mutants in Dean et al., 2020. We assumed that the initial population was dominated by ancestral cells ($\alpha =\beta =1$) but also included a subpopulation of resistant mutants whose scaling parameters were uniformly sampled from those measured across multiple days of the evolution (see Materials and methods). The model predicts different trajectories for different external doses (selective regimes: red to blue, Figure 5, top panel), leading to dramatically different changes in resistance (IC₅₀) and growth rate adaptation (Figure 5, bottom panels), similar to those observed in experiment. Specifically, the model predicts (and experiments confirm) a dramatic decrease in CIP resistance as TGC concentration is increased along a contour of constant growth. As TGC eclipses acritical concentration of approximately 0.025 g/mL, selection for both CIP resistance and increased growth is eliminated. We note that comparisons between the model and experiment involve no adjustable parameters, and while the model captures the qualitative trends, it slightly but systematically underestimates the growth across TGC concentrations – perhaps suggesting additional evolutionary dynamics not captured by the model. Intuitively, the experimental results can be explained by the strongly antagonistic interaction between the two drugs, which reverses the selection for resistance to CIP at sufficiently high TGC concentrations (compare CIP resistance at TGC = 0 and TGC = 0.04 g/mL, which both involve CIP ≈ 0.2 g/mL; similar results have been seen in other species and for other drug combinations; Chait et al., 2007). We also compared model predictions with experimental adaptation to two additional drug pairs (Figure 5—figure supplements 1 and 2) and again found that the model captures the qualitative features of both resistance changes to the component drugs and growth adaptation.

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Effects of mutations

While we have focused on selection dynamics, the model can be extended to include mutation events linking different phenotypes (i.e., linking different pairs of scaling parameters). These mutational pathways may be necessary to capture certain evolutionary features – for example, historical contingencies between sequentially acquired mutations (Barbosa et al., 2017; Card et al., 2019; Das et al., 2020). To incorporate mutational structure and processes, we modify the equation for each subpopulation $n_i$ to

(12) ${\displaystyle \frac{d{n}_i}{dt}=g_i{n}_i-\mu n_i+\mu \sum _j^M{m}_{ji}{n}_j}$

where μ is the mutation rate and $m_{ji}$ is the probability that strain $j$ mutates to strain $i$, given that there is a mutation in strain $j$. We will refer to the matrix formed by the parameters $m_{ji}$ as the mutation matrix as it contains information about allowed mutational trajectories linking different phenotypes (Day and Gandon, 2006). The Price equation for the mean traits (the mean scaling parameters) now becomes

(13) ${\displaystyle \begin{array}{l}{\displaystyle \frac{d\overline{\alpha }}{dt}={\mathrm{C}\mathrm{o}\mathrm{v}}_{\mathit{x}}(\alpha ,g)-\mu (\overline{\alpha }-{\overline{\alpha }}_m),}\\ \frac{d\overline{\beta }}{dt}={\mathrm{C}\mathrm{o}\mathrm{v}}_{\mathit{x}}(\beta ,g)-\mu (\overline{\beta }-{\overline{\beta }}_m),\end{array}}$

where ${\overline{\alpha }}_m$ and ${\overline{\beta }}_m$ are the mean trait values in all mutants that arise, and are given by

(14) ${\displaystyle \begin{array}{l}{\overline{\alpha }}_m=\sum _{ij}{\alpha }_i{m}_{ji}{f}_j,\\ {\overline{\beta }}_m=\sum _{ij}{\beta }_i{m}_{ji}{f}_j.ll\end{array}}$

When the mutation matrix has a particularly simple structure, the effects of mutation will be similar to those of selection. For example, when mutations occur with uniform probability between the ancestral strain and each mutant, the evolutionary dynamics are qualitatively similar to those in the absence of mutation (Figure 6—figure supplement 1, compare to Figure 4). On the other hand, certain mutational structures can lead to new behavior. As an example, we consider a toy model consisting of four phenotypes defined by the level of resistance (sensitive, S, or resistant, R) to one of two drugs. The phenotypes are designated SS $(\alpha =1,\beta =1)$, RS $(\alpha =1/5,\beta =1)$, SR $(\alpha =1,\beta =1/5)$, or RR $(\alpha =1/5,\beta =1/5)$. Note that these phenotypes do not assume, a priori, any particular relationships between the underlying genotypes. For example, each phenotype could correspond to a single mutation – in which case the RR phenotype would be said to exhibit strong cross-resistance – or alternatively, the phenotypes could correspond to single drug mutants (SR and RS) and double mutants (RR), which implies a particular sequence in which the mutations can be acquired. These two situations would lead to significant differences in the expected structure of the mutational matrix and, as we will see, in the evolutionary dynamics.

For simplicity, we consider two different mutational structures: one corresponding to direct and uniform pathways between the ancestor (SS) and all mutant phenotypes (SR, RS, and RR), and the second corresponding to sequential pathways from ancestor to single mutants (SR, RS) and then from single mutants to double mutants (Figure 6). To illustrate the impact of mutational structure, we consider two drugs that interact antagonistically and choose the external drug concentrations such that $D_1>D_2$ (Figure 6C). While both mutational structures lead eventually to a population of double-resistant phenotypes (RR), the sequential pathway leads to slower adaptation of growth as the population evolves first toward the most fit single mutant (in this case, RS because the drug combination contains a higher concentration of drug 1) before eventually arriving at RR (Figure 6C–E). The specific trajectory will of course depend on both the drug interaction (the shape of the growth contours) and the specific dosage combination (see Figure 6—figure supplement 2).

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There are other types of mutational constraints that can be implemented in the formalism, including explicit functional dependencies between different antibiotic resistance phenotypes. As an example, we assumed a distance-based mutation matrix where the mutation probability between different strains depends on the Euclidean distance between their scaling parameter pairs (${\alpha }_i,{\beta }_i$) according to $m_{ji}\sim e^{-d_{ji}}$, where $d_{ji}=\sqrt{{({\alpha }_i-{\alpha }_j)}^2+{({\beta }_i-{\beta }_j)}^2}$. Intuitively, this structure means that mutations are more likely between strains with similar rescaling parameters (and therefore similar levels of resistance to the component drugs). As expected, this constrained mutational structure leads to slower growth rate adaptation than a model with a simple uniform mutation model (Figure 6—figure supplement 3). These effects are further compounded by statistical properties of the collateral structures (e.g., positive or negative correlations between the possible αs and βs; Figure 3), the specific (stochastic) realization of those scaling parameters (Figure 6—figure supplement 4), and the precise shape of two-drug growth surface. Note, however, that these dynamics depend fundamentally on the global mutation rate μ, which modulates the relative balance between selection and mutation in a given environment.

Evolutionary dynamics under temporal sequences of drug combinations

Evolutionary dynamics in the presence of multiple drugs can be extremely complex. While past work has focused primarily on the use of either temporal sequences or (simultaneous) combinations of antibiotics, more complex scenarios are possible, in principle, but difficult to analyze even in theoretical models. The simplicity of the Price equation framework allows us to investigate evolutionary dynamics in response to a more complex scenario: temporal sequences of antibiotic combinations. As proof of principle, we numerically studied time-dependent therapies consisting of two sequential regimes (treatment A and treatment B; Figure 7) characterized by different dosage combinations of tigecycline (drug 1) and ciprofloxacin (drug 2) (the growth surface was measured in Dean et al., 2020). The two dosage combinations were chosen to lie along a contour of constant growth (Figure 7A), meaning that the net inhibitory effects of A and B are the same when applied to ancestral cells. Using experimental estimates for $\alpha ,\beta$ (Figure 3—figure supplement 1C), we find that both the resistance levels to the two drugs and the growth rate increase during treatment, as one might expect. However, the dynamics of these changes depend on both the relative duration of each treatment and total treatment length (Figure 7—figure supplements 1 and 2). For example, consider a treatment of total length $T$ consisting of an initial period of treatment A followed by a final period of treatment B. If we vary the relative length of the two epochs while keeping the total treatment length fixed, we find that the resistance to each drug and the growth rate increase monotonically as the fraction of time in B increases (Figure 7H–J). This is a consequence of the interplay between the distribution of available mutants and the shape of the growth surface under these particular two drugs; the mutants tend to feature higher levels of resistance to drug 2 than drug 1, yet the benefits of this resistance – that is, the increase in growth rate due to rescaling the concentration of drug 2 – are favored much more so under condition B than condition A (where rescaling tends to produce effective drug concentrations that lie near the original growth contour). It is notable, however, that effects (both resistance levels and final growth) change nonlinearly as the fraction of time in B is increased – that is, even in this simple model, the effects of a two-epoch (A then B) treatment cannot be inferred as a simple linear interpolation between the effects of A-only and B-only treatments.

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Antibiotic resistance is a growing threat to modern medicine and public health. Multidrug therapies are a promising approach to both increase efficacy of treatment and prevent evolution of resistance, but the two effects can be coupled in counterintuitive ways through the drug interactions and collateral effects linking the component drugs. Our results provide a unified framework for incorporating both drug interactions and collateral effects to predict phenotypic adaptation on coarse-grained fitness landscapes that are measurable features of the ancestral population (Table 1). Special cases of the model reproduce previous experimental results that appear, on the surface, to be contradictory; indeed, adaptation can be driven primarily by drug interactions, primarily by collateral effects, or by a combination of both, and the balance of these effects can be shifted using tunable properties of the system (i.e., the ratio of drug dosages). Our model was inspired by rescaling arguments that were originally introduced in Chait et al., 2007 and have since been shown to capture phenotypic features of dose-response surfaces or adaptation in multiple bacterial species (Michel et al., 2008; Hegreness et al., 2008; Torella et al., 2010; Wood and Cluzel, 2012; Wood et al., 2014; Dean et al., 2020; Das et al., 2020). Our results complement these studies by showing how similar rescaling assumptions, when formalized in a population dynamics model, lead to testable predictions for the dynamics of both growth adaptation and phenotype (resistance) evolution. Importantly, the model also has a simple intuitive explanation, with evolutionary trajectories driven by weighted gradient dynamics on two-dimensional landscapes, where the local geometry of the landscape reflects the drug interaction and collateral effects constrain the direction of motion. We have also illustrated how de novo mutation can be integrated in the same framework, provided the mutational structure among a pool of possible phenotypes is known or if specific assumptions are made regarding functional links and constraints for shifts between different resistance levels. Mutation at constant rate is indeed a classic version of the complete Price equation. However, more complex mutational effects – including stress-dependent modulation of mutation rate (Kohanski et al., 2010; Vasse et al., 2020) – could be included as a more flexible tunable term in Equation 5.

It is important to keep in mind several limitations of our approach. The primary rescaling assumption of the model is that growth of a drug-resistant mutant is well approximated by growth of the ancestral strain at a new ‘effective’ drug concentration – one that differs from the true external concentration. This approximation has considerable empirical support (Chait et al., 2007; Wood and Cluzel, 2012; Wood et al., 2014; Das et al., 2020) but is not expected to always hold; indeed, there are examples where mutations lead to more complex nonlinear transformations of the drug-response surface (Wood et al., 2014; Munck et al., 2014). In addition, it is possible that selection can act on some other feature of the dose-response curve characterizing single-drug effects – modulating, for example, its steepness (rather than merely its scale). While these effects could in principle be incorporated into our model – for example, by assuming transformations of the ancestral surface, perhaps occurring on a slower timescale, that go beyond simple rescaling – we have not focused on those cases. For simplicity, we have also neglected a number of features that may impact microbial evolution. For example, we have assumed that different subpopulations grow exponentially, neglecting potential interactions including clonal interference (Gerrish and Lenski, 1998), intercellular (Koch et al., 2014; Hansen et al., 2017; Hansen et al., 2020) or intra-lineage (Ogbunugafor and Eppstein, 2017) competition, and cooperation (Yurtsev et al., 2013; Sorg et al., 2016; Estrela and Brown, 2018; Frost et al., 2018; Hallinen et al., 2020), as well as potential effects of demographic noise and population extinction (Coates et al., 2018). These complexities could also be incorporated in our model, perhaps at the expense of some intuitive interpretations (e.g., weighted gradient dynamics) that currently apply. In addition, we have not explicitly included a fitness cost of resistance (Andersson and Hughes, 2010) – that is, we assume that growth rates of mutants and ancestral cells are identical in the absence of drug. This assumption could be relaxed by including a prefactor to the growth function, $g_i\to (1-{\gamma }_i({\alpha }_i,{\beta }_i))g_i$, where ${\gamma }_i({\alpha }_i,{\beta }_i)$ is the cost of resistance, which in general depends on the scaling parameters (if not, it can be easily incorporated as a constant). While such fitness costs have traditionally been seen as essential for reversing resistance (with, e.g., drug-free ‘holidays’; Dunai et al., 2019), our results underscore the idea that reversing adaptation relies on differential fitness along a multidimensional continuum of environments, not merely binary (drug/no drug) conditions. Our results indicate resistance and bacterial growth can be significantly constrained by optimal tuning of multidrug environments, even in the absence of fitness cost. Finally, our model deals only with heritable resistance and therefore may not capture phenotypic affects associated with, for example, transient resistance (El Meouche and Dunlop, 2018) or cellular hysteresis (Roemhild et al., 2018).

Our goal was to strike a balance between analytical tractability and generality vs. biological realism and system specificity. But we stress that the predictions of this model do not come for free; they depend, for example, on properties of the dose-response surfaces, the collection of scaling parameters, and the specific mutational structure. In many cases, these features can be determined empirically; in other cases, some or all of these properties may be unknown. The evolutionary predictions of the model will ultimately depend on these inputs, and it is difficult to draw general conclusions (e.g., 'synergistic combinations always accelerate resistance') that apply across all classes of drug combinations, collateral effects, and mutational structures. But we believe the framework is valuable precisely because it generates testable predictions in situations that might otherwise seem intractably complex.

Our approach also has pedagogical value as it connects evolutionary effects of drug combinations and collateral effects with well-established concepts in evolutionary biology and population genetics (Price, 1970; Price, 1972; Day and Gandon, 2006). Approximations similar to Equation 9 have been derived previously in quantitative genetics models (Abrams et al., 1993; Taylor, 1996) and other applications of the Price equation (Lehtonen, 2018). While the gradient approximation does not require that the population be monomorphic with rare mutants or a particular form for the phenotype distribution, it does require that the majority of trait values (here scaling parameters) are contained in a regime where $G(x,y)$ is approximately linear; in our case, that linearity arises by Taylor expansion and neglecting higher-order deviations from the population mean. More generally, the direction of evolutionary change in our model is determined by the gradient of the fitness function $\nabla G$ evaluated at the mean trait $(\overline{\alpha },\overline{\beta })$; when the gradient vanishes, this point corresponds to a singular point (Waxman and Gavrilets, 2005) or an evolutionarily singular strategy (Geritz et al., 1998). Whether such point may be reached (convergence stability) and how much variance in trait values can be maintained around such point (whether evolutionarily stable [ESS]) depend on other features, such as higher-order derivatives (Otto and Day, 2011; Eshel et al., 1997; Lehtonen, 2018; Smith, 1982; Parker and Smith, 1990). In the case of drug interactions, the fitness landscape in $(\alpha ,\beta )$ space will typically have a single maximum at (0, 0) corresponding to effective drug concentrations of zero. However, whether that point is reachable in general or within a given time frame will depend on the available mutants preexisting at very low frequencies in the population, or on the speed and biases in the mutational process itself, if such mutants are to be generated de novo during treatment. In principle, the model also allows for long-term coexistence between different strains; in that case, the rescaled effective drug concentrations experienced by both strains would fall along a single contour of constant growth. Hence, while variance in the population growth will necessarily decrease over time, variance in the traits (scaling parameters) themselves can change non-monotonically.

The framework is sufficiently flexible to integrate different strands of empirical data, and our results underscore the need for quantitative phenotype data tracking resistance to multiple drugs simultaneously, especially when drug combinations are potentially driving selection dynamics. At an epidemiological level, the dominant approach in describing resistance has been to use fixed breakpoints in MIC and track the percentage of isolates with MIC above (drug-resistant) or below (drug-sensitive) such breakpoint (e.g., ECDC, 2019; Chang et al., 2015). By missing or decoupling patterns of co-occurrence between MICs to different drugs across isolates, this approach remains incomplete for mechanistic predictions. Our framework suggests that going beyond such binary description towards a more continuous and multidimensional phenotype characterization of drug resistance is possible, with applications not just in microbiology but also in the evolutionary epidemiology of drug resistance (Day and Gandon, 2012; Day et al., 2020). In the long run, these advances may yield better and more precise predictions of resistance evolution at multiple scales, and, in turn, optimized treatments that balance short-term inhibitory effects of a drug cocktail with its inseparable, longer-term evolutionary consequences.

Perhaps most importantly, our approach provides a low-dimensional approximation to the high-dimensional dynamics governing the evolution of resistance. In contrast to the classical genotype-centric approaches to resistance, our model uses rescaling arguments to connect measurable traits of resistant cells (scaling parameters) to environment-dependent phenotypes (growth). This rescaling dramatically reduces the complexity of the problem as the two-drug-response surfaces – and, effectively, the fitness landscape – can be estimated from only single-drug dose-response curves. Such coarse-grained models can help extract simplifying principles from otherwise intractable complexity (Shoval et al., 2012; Hart et al., 2015). In many ways, the classical Price equation performs a similar function, revealing links between trait-fitness covariance and selection that, at a mathematical level, are already embedded in simple models of population growth. In the case of multidrug resistance, this formalism reveals that drug interactions and collateral effects are not independent features of resistance evolution, and neither, alone, can provide a complete picture. Instead, they are coupled through local geometry of the two-drug-response surface, and we show how specific dosage combinations can shift the weighting of these two effects, providing a framework for systematic optimization of time-dependent multidrug therapies.

Data used in this paper was taken from a public repository: Dean, Ziah; Maltas, Jeff; Wood, Kevin (2020), Antibiotic interactions shape short-term evolution of resistance in Enterococcus faecalis, Dryad, Dataset, https://doi.org/10.5061/dryad.j3tx95x92 There are no restrictions on any new results.

1. 1. Ziah D
   2. Maltas J
   3. Wood K

   (2020) Dryad Digital Repository

   Data from: Antibiotic interactions shape short-term evolution of resistance in Enterococcus faecalis.

   https://doi.org/10.5061/dryad.j3tx95x92

1. 1. Abrams PA
   2. Harada Y
   3. Matsuda H

   (1993) On the relationship between quantitative genetic and ess models

   Evolution 47:982–985.

   https://doi.org/10.2307/2410204

   • Google Scholar
2. 1. Adamowicz EM
   2. Flynn J
   3. Hunter RC
   4. Harcombe WR

   (2018) Cross-feeding modulates antibiotic tolerance in bacterial communities

   The ISME Journal 12:2723–2735.

   https://doi.org/10.1038/s41396-018-0212-z

   • PubMed
   • Google Scholar
3. 1. Adamowicz EM
   2. Muza M
   3. Chacón JM
   4. Harcombe WR

   (2020) Cross-feeding modulates the rate and mechanism of antibiotic resistance evolution in a model microbial community of Escherichia coli and Salmonella enterica

   PLOS Pathogens 16:e1008700.

   https://doi.org/10.1371/journal.ppat.1008700

   • PubMed
   • Google Scholar
4. 1. Andersson DI
   2. Hughes D

   (2010) Antibiotic resistance and its cost: is it possible to reverse resistance?

   Nature Reviews Microbiology 8:260–271.

   https://doi.org/10.1038/nrmicro2319

   • PubMed
   • Google Scholar
5. 1. Apjok G
   2. Boross G
   3. Nyerges Á
   4. Fekete G
   5. Lázár V
   6. Papp B
   7. Pál C
   8. Csörgő B

   (2019) Limited evolutionary conservation of the phenotypic effects of antibiotic resistance mutations

   Molecular Biology and Evolution 36:1601–1611.

   https://doi.org/10.1093/molbev/msz109

   • PubMed
   • Google Scholar
6. Preprint

   1. Ardell SM
   2. Kryazhimskiy S

   (2020) The population genetics of pleiotropy, and the evolution of collateral resistance and sensitivity in Bacteria

   bioRxiv.

   https://doi.org/10.1101/2020.08.25.267484

   • Google Scholar
7. 1. Artemova T
   2. Gerardin Y
   3. Dudley C
   4. Vega NM
   5. Gore J

   (2015) Isolated cell behavior drives the evolution of antibiotic resistance

   Molecular Systems Biology 11:822.

   https://doi.org/10.15252/msb.20145888

   • PubMed
   • Google Scholar
8. 1. Baltekin Ö
   2. Boucharin A
   3. Tano E
   4. Andersson DI
   5. Elf J

   (2017) Antibiotic susceptibility testing in less than 30 min using direct single-cell imaging

   PNAS 114:9170–9175.

   https://doi.org/10.1073/pnas.1708558114

   • PubMed
   • Google Scholar
9. 1. Barbosa C
   2. Trebosc V
   3. Kemmer C
   4. Rosenstiel P
   5. Beardmore R
   6. Schulenburg H
   7. Jansen G

   (2017) Alternative evolutionary paths to bacterial antibiotic resistance cause distinct collateral effects

   Molecular Biology and Evolution 34:2229–2244.

   https://doi.org/10.1093/molbev/msx158

   • PubMed
   • Google Scholar
10. 1. Barbosa C
    2. Beardmore R
    3. Schulenburg H
    4. Jansen G

    (2018) Antibiotic combination efficacy (ACE) networks for a Pseudomonas aeruginosa model

    PLOS Biology 16:e2004356.

    https://doi.org/10.1371/journal.pbio.2004356

    • PubMed
    • Google Scholar
11. 1. Barbosa C
    2. Römhild R
    3. Rosenstiel P
    4. Schulenburg H

    (2019) Evolutionary stability of collateral sensitivity to antibiotics in the model pathogen Pseudomonas aeruginosa

    eLife 8:e51481.

    https://doi.org/10.7554/eLife.51481

    • PubMed
    • Google Scholar
12. 1. Baym M
    2. Lieberman TD
    3. Kelsic ED
    4. Chait R
    5. Gross R
    6. Yelin I
    7. Kishony R

    (2016a) Spatiotemporal microbial evolution on antibiotic landscapes

    Science 353:1147–1151.

    https://doi.org/10.1126/science.aag0822

    • PubMed
    • Google Scholar
13. 1. Baym M
    2. Stone LK
    3. Kishony R

    (2016b) Multidrug evolutionary strategies to reverse antibiotic resistance

    Science 351:aad3292.

    https://doi.org/10.1126/science.aad3292

    • PubMed
    • Google Scholar
14. 1. Beardmore RE
    2. Peña-Miller R
    3. Gori F
    4. Iredell J

    (2017) Antibiotic cycling and antibiotic mixing: which one best mitigates antibiotic resistance?

    Molecular Biology and Evolution 34:msw292–817.

    https://doi.org/10.1093/molbev/msw292

    • PubMed
    • Google Scholar
15. 1. Bergstrom CT
    2. Lo M
    3. Lipsitch M

    (2004) Ecological theory suggests that antimicrobial cycling will not reduce antimicrobial resistance in hospitals

    PNAS 101:13285–13290.

    https://doi.org/10.1073/pnas.0402298101

    • PubMed
    • Google Scholar
16. 1. Bollenbach T
    2. Quan S
    3. Chait R
    4. Kishony R

    (2009) Nonoptimal microbial response to antibiotics underlies suppressive drug interactions

    Cell 139:707–718.

    https://doi.org/10.1016/j.cell.2009.10.025

    • PubMed
    • Google Scholar
17. 1. Borrell S
    2. Teo Y
    3. Giardina F
    4. Streicher EM
    5. Klopper M
    6. Feldmann J
    7. Müller B
    8. Victor TC
    9. Gagneux S

    (2013) Epistasis between antibiotic resistance mutations drives the evolution of extensively drug-resistant tuberculosis

    Evolution, Medicine, and Public Health 2013:65–74.

    https://doi.org/10.1093/emph/eot003

    • PubMed
    • Google Scholar
18. 1. Card KJ
    2. LaBar T
    3. Gomez JB
    4. Lenski RE

    (2019) Historical contingency in the evolution of antibiotic resistance after decades of relaxed selection

    PLOS Biology 17:e3000397.

    https://doi.org/10.1371/journal.pbio.3000397

    • PubMed
    • Google Scholar
19. 1. Chait R
    2. Craney A
    3. Kishony R

    (2007) Antibiotic interactions that select against resistance

    Nature 446:668–671.

    https://doi.org/10.1038/nature05685

    • PubMed
    • Google Scholar
20. 1. Chang HH
    2. Cohen T
    3. Grad YH
    4. Hanage WP
    5. O'Brien TF
    6. Lipsitch M

    (2015) Origin and proliferation of multiple-drug resistance in bacterial pathogens

    Microbiology and Molecular Biology Reviews 79:101–116.

    https://doi.org/10.1128/MMBR.00039-14

    • PubMed
    • Google Scholar
21. 1. Chevereau G
    2. Dravecká M
    3. Batur T
    4. Guvenek A
    5. Ayhan DH
    6. Toprak E
    7. Bollenbach T

    (2015) Quantifying the determinants of evolutionary dynamics leading to drug resistance

    PLOS Biology 13:e1002299.

    https://doi.org/10.1371/journal.pbio.1002299

    • PubMed
    • Google Scholar
22. 1. Coates J
    2. Park BR
    3. Le D
    4. Şimşek E
    5. Chaudhry W
    6. Kim M

    (2018) Antibiotic-induced population fluctuations and stochastic clearance of Bacteria

    eLife 7:e32976.

    https://doi.org/10.7554/eLife.32976

    • Google Scholar
23. 1. Cooper RM
    2. Tsimring L
    3. Hasty J

    (2017) Inter-species population dynamics enhance microbial horizontal gene transfer and spread of antibiotic resistance

    eLife 6:e25950.

    https://doi.org/10.7554/eLife.25950

    • PubMed
    • Google Scholar
24. 1. Das SG
    2. Direito SOL
    3. Waclaw B
    4. Allen RJ
    5. Krug J

    (2020) Predictable properties of fitness landscapes induced by adaptational tradeoffs

    eLife 9:e55155.

    https://doi.org/10.7554/eLife.55155

    • Google Scholar
25. 1. Davies J
    2. Davies D

    (2010) Origins and evolution of antibiotic resistance

    Microbiology and Molecular Biology Reviews 74:417–433.

    https://doi.org/10.1128/MMBR.00016-10

    • PubMed
    • Google Scholar
26. 1. Day T
    2. Parsons T
    3. Lambert A
    4. Gandon S

    (2020) The price equation and evolutionary epidemiology

    Philosophical Transactions of the Royal Society B: Biological Sciences 375:20190357.

    https://doi.org/10.1098/rstb.2019.0357

    • Google Scholar
27. 1. Day T
    2. Gandon S

    (2006) Insights from price's equation into evolutionary

    Disease Evolution: Models, Concepts, and Data Analyses 71:23.

    https://doi.org/10.1090/dimacs/071/02

    • Google Scholar
28. 1. Day T
    2. Gandon S

    (2012) The evolutionary epidemiology of multilocus drug resistance

    Evolution 66:1582–1597.

    https://doi.org/10.1111/j.1558-5646.2011.01533.x

    • Google Scholar
29. 1. De Jong MG
    2. Wood KB

    (2018) Tuning spatial profiles of selection pressure to modulate the evolution of drug resistance

    Physical Review Letters 120:238102.

    https://doi.org/10.1103/PhysRevLett.120.238102

    • PubMed
    • Google Scholar
30. 1. de Visser JA
    2. Krug J

    (2014) Empirical fitness landscapes and the predictability of evolution

    Nature Reviews Genetics 15:480–490.

    https://doi.org/10.1038/nrg3744

    • PubMed
    • Google Scholar
31. 1. Dean Z
    2. Maltas J
    3. Wood KB

    (2020) Antibiotic interactions shape short-term evolution of resistance in E. faecalis

    PLOS Pathogens 16:e1008278.

    https://doi.org/10.1371/journal.ppat.1008278

    • PubMed
    • Google Scholar
32. 1. Dunai A
    2. Spohn R
    3. Farkas Z
    4. Lázár V
    5. Györkei Ádám
    6. Apjok G
    7. Boross G
    8. Szappanos B
    9. Grézal G
    10. Faragó A
    11. Bodai L
    12. Papp B
    13. Pál C

    (2019) Rapid decline of bacterial drug-resistance in an antibiotic-free environment through phenotypic reversion

    eLife 8:e47088.

    https://doi.org/10.7554/eLife.47088

    • Google Scholar
33. Report

    1. ECDC

    (2019)

    Surveillance of Antimicrobial Resistance in Europe 2018

    ECDC.

    • Google Scholar
34. 1. El Meouche I
    2. Dunlop MJ

    (2018) Heterogeneity in efflux pump expression predisposes antibiotic-resistant cells to mutation

    Science 362:686–690.

    https://doi.org/10.1126/science.aar7981

    • PubMed
    • Google Scholar
35. 1. Engelstädter J

    (2014) Fitness landscapes emerging from pharmacodynamic functions in the evolution of multidrug resistance

    Journal of Evolutionary Biology 27:840–853.

    https://doi.org/10.1111/jeb.12355

    • PubMed
    • Google Scholar
36. 1. Eshel I
    2. Motro U
    3. Sansone E

    (1997) Continuous stability and evolutionary convergence

    Journal of Theoretical Biology 185:333–343.

    https://doi.org/10.1006/jtbi.1996.0312

    • Google Scholar
37. 1. Estrela S
    2. Brown SP

    (2018) Community interactions and spatial structure shape selection on antibiotic resistant lineages

    PLOS Computational Biology 14:e1006179.

    https://doi.org/10.1371/journal.pcbi.1006179

    • PubMed
    • Google Scholar
38. 1. Frank SA

    (1995) George price's contributions to evolutionary genetics

    Journal of Theoretical Biology 175:373–388.

    https://doi.org/10.1006/jtbi.1995.0148

    • PubMed
    • Google Scholar
39. 1. Frost I
    2. Smith WPJ
    3. Mitri S
    4. Millan AS
    5. Davit Y
    6. Osborne JM
    7. Pitt-Francis JM
    8. MacLean RC
    9. Foster KR

    (2018) Cooperation, competition and antibiotic resistance in bacterial colonies

    The ISME Journal 12:1582–1593.

    https://doi.org/10.1038/s41396-018-0090-4

    • PubMed
    • Google Scholar
40. 1. Fuentes-Hernandez A
    2. Plucain J
    3. Gori F
    4. Pena-Miller R
    5. Reding C
    6. Jansen G
    7. Schulenburg H
    8. Gudelj I
    9. Beardmore R

    (2015) Using a sequential regimen to eliminate Bacteria at sublethal antibiotic dosages

    PLOS Biology 13:e1002104.

    https://doi.org/10.1371/journal.pbio.1002104

    • PubMed
    • Google Scholar
41. 1. Geritz SAH
    2. Kisdi É.
    3. Meszéna G
    4. Metz JAJ

    (1998) Evolutionarily singular strategies and the adaptive growth and branching of the evolutionary tree

    Evolutionary Ecology 12:35–57.

    https://doi.org/10.1023/A:1006554906681

    • Google Scholar
42. 1. Gerrish PJ
    2. Lenski RE

    (1998) The fate of competing beneficial mutations in an asexual population

    Genetica 102-103:127–144.

    https://doi.org/10.1023/A:1017067816551

    • PubMed
    • Google Scholar
43. 1. Gjini E
    2. Brito PH

    (2016) Integrating antimicrobial therapy with host immunity to fight Drug-Resistant infections: classical vs. adaptive treatment

    PLOS Computational Biology 12:e1004857.

    https://doi.org/10.1371/journal.pcbi.1004857

    • PubMed
    • Google Scholar
44. 1. Gokhale S
    2. Conwill A
    3. Ranjan T
    4. Gore J

    (2018) Migration alters oscillatory dynamics and promotes survival in connected bacterial populations

    Nature Communications 9:1–10.

    https://doi.org/10.1038/s41467-018-07703-y

    • Google Scholar
45. 1. Greco WR
    2. Bravo G
    3. Parsons JC

    (1995)

    The search for synergy: a critical review from a response surface perspective

    Pharmacological Reviews 47:331–385.

    • PubMed
    • Google Scholar
46. 1. Greulich P
    2. Waclaw B
    3. Allen RJ

    (2012) Mutational pathway determines whether drug gradients accelerate evolution of drug-resistant cells

    Physical Review Letters 109:088101.

    https://doi.org/10.1103/PhysRevLett.109.088101

    • PubMed
    • Google Scholar
47. 1. Hallinen KM
    2. Karslake J
    3. Wood KB

    (2020) Delayed antibiotic exposure induces population collapse in enterococcal communities with drug-resistant subpopulations

    eLife 9:e52813.

    https://doi.org/10.7554/eLife.52813

    • PubMed
    • Google Scholar
48. 1. Hansen E
    2. Woods RJ
    3. Read AF

    (2017) How to use a chemotherapeutic agent when resistance to it threatens the patient

    PLOS Biology 15:e2001110.

    https://doi.org/10.1371/journal.pbio.2001110

    • Google Scholar
49. 1. Hansen E
    2. Karslake J
    3. Woods RJ
    4. Read AF
    5. Wood KB

    (2020) Antibiotics can be used to contain drug-resistant Bacteria by maintaining sufficiently large sensitive populations

    PLOS Biology 18:e3000713.

    https://doi.org/10.1371/journal.pbio.3000713

    • PubMed
    • Google Scholar
50. 1. Hart Y
    2. Sheftel H
    3. Hausser J
    4. Szekely P
    5. Ben-Moshe NB
    6. Korem Y
    7. Tendler A
    8. Mayo AE
    9. Alon U

    (2015) Inferring biological tasks using pareto analysis of high-dimensional data

    Nature Methods 12:233–235.

    https://doi.org/10.1038/nmeth.3254

    • PubMed
    • Google Scholar
51. 1. Hegreness M
    2. Shoresh N
    3. Damian D
    4. Hartl D
    5. Kishony R

    (2008) Accelerated evolution of resistance in multidrug environments

    PNAS 105:13977–13981.

    https://doi.org/10.1073/pnas.0805965105

    • PubMed
    • Google Scholar
52. 1. Hermsen R
    2. Deris JB
    3. Hwa T

    (2012) On the rapidity of antibiotic resistance evolution facilitated by a concentration gradient

    PNAS 109:10775–10780.

    https://doi.org/10.1073/pnas.1117716109

    • Google Scholar
53. 1. Hernando-Amado S
    2. Sanz-García F
    3. Martínez JL

    (2020) Rapid and robust evolution of collateral sensitivity in Pseudomonas aeruginosa antibiotic-resistant mutants

    Science Advances 6:eaba5493.

    https://doi.org/10.1126/sciadv.aba5493

    • Google Scholar
54. 1. Imamovic L
    2. Ellabaan MMH
    3. Dantas Machado AM
    4. Citterio L
    5. Wulff T
    6. Molin S
    7. Krogh Johansen H
    8. Sommer MOA

    (2018) Drug-Driven phenotypic convergence supports rational treatment strategies of chronic infections

    Cell 172:121–134.

    https://doi.org/10.1016/j.cell.2017.12.012

    • PubMed
    • Google Scholar
55. 1. Imamovic L
    2. Sommer MO

    (2013) Use of collateral sensitivity networks to design drug cycling protocols that avoid resistance development

    Science Translational Medicine 5:204ra132.

    https://doi.org/10.1126/scitranslmed.3006609

    • PubMed
    • Google Scholar
56. 1. Iram S
    2. Dolson E
    3. Chiel J
    4. Pelesko J
    5. Krishnan N
    6. Güngör Ö
    7. Kuznets-Speck B
    8. Deffner S
    9. Ilker E
    10. Scott JG
    11. Hinczewski M

    (2021) Controlling the speed and trajectory of evolution with counterdiabatic driving

    Nature Physics 17:135–142.

    https://doi.org/10.1038/s41567-020-0989-3

    • Google Scholar
57. 1. Karslake J
    2. Maltas J
    3. Brumm P
    4. Wood KB

    (2016) Population density modulates drug inhibition and gives rise to potential bistability of treatment outcomes for bacterial infections

    PLOS Computational Biology 12:e1005098.

    https://doi.org/10.1371/journal.pcbi.1005098

    • PubMed
    • Google Scholar
58. 1. Kim S
    2. Lieberman TD
    3. Kishony R

    (2014) Alternating antibiotic treatments constrain evolutionary paths to multidrug resistance

    PNAS 111:14494–14499.

    https://doi.org/10.1073/pnas.1409800111

    • Google Scholar
59. 1. Koch G
    2. Yepes A
    3. Förstner KU
    4. Wermser C
    5. Stengel ST
    6. Modamio J
    7. Ohlsen K
    8. Foster KR
    9. Lopez D

    (2014) Evolution of resistance to a last-resort antibiotic in Staphylococcus aureus via bacterial competition

    Cell 158:1060–1071.

    https://doi.org/10.1016/j.cell.2014.06.046

    • PubMed
    • Google Scholar
60. 1. Kohanski MA
    2. DePristo MA
    3. Collins JJ

    (2010) Sublethal antibiotic treatment leads to multidrug resistance via radical-induced mutagenesis

    Molecular Cell 37:311–320.

    https://doi.org/10.1016/j.molcel.2010.01.003

    • PubMed
    • Google Scholar
61. 1. Lehtonen J

    (2018) The price equation, gradient dynamics, and continuous trait game theory

    The American Naturalist 191:146–153.

    https://doi.org/10.1086/694891

    • PubMed
    • Google Scholar
62. 1. Lehtonen J
    2. Okasha S
    3. Helanterä H

    (2020) Fifty years of the price equation

    Philosophical Transactions of the Royal Society B: Biological Sciences 375:20190350.

    https://doi.org/10.1098/rstb.2019.0350

    • Google Scholar
63. 1. Levy SB
    2. Marshall B

    (2004) Antibacterial resistance worldwide: causes, challenges and responses

    Nature Medicine 10:S122–S129.

    https://doi.org/10.1038/nm1145

    • PubMed
    • Google Scholar
64. 1. Lipsitch M
    2. Levin BR

    (1997) The population dynamics of antimicrobial chemotherapy

    Antimicrobial Agents and Chemotherapy 41:363–373.

    https://doi.org/10.1128/AAC.41.2.363

    • Google Scholar
65. 1. Loewe S

    (1953)

    The problem of synergism and antagonism of combined drugs

    Arzneimittel-Forschung 3:285–290.

    • PubMed
    • Google Scholar
66. 1. Lopatkin AJ
    2. Huang S
    3. Smith RP
    4. Srimani JK
    5. Sysoeva TA
    6. Bewick S
    7. Karig DK
    8. You L

    (2016) Antibiotics as a selective driver for conjugation dynamics

    Nature Microbiology 1:16044.

    https://doi.org/10.1038/nmicrobiol.2016.44

    • PubMed
    • Google Scholar
67. 1. Lopatkin AJ
    2. Meredith HR
    3. Srimani JK
    4. Pfeiffer C
    5. Durrett R
    6. You L

    (2017) Persistence and reversal of plasmid-mediated antibiotic resistance

    Nature Communications 8:1689.

    https://doi.org/10.1038/s41467-017-01532-1

    • PubMed
    • Google Scholar
68. 1. Lukačišin M
    2. Bollenbach T

    (2019) Emergent gene expression responses to drug combinations predict Higher-Order drug interactions

    Cell Systems 9:423–433.

    https://doi.org/10.1016/j.cels.2019.10.004

    • PubMed
    • Google Scholar
69. 1. Lukačišinová M
    2. Fernando B
    3. Bollenbach T

    (2020) Highly parallel lab evolution reveals that epistasis can curb the evolution of antibiotic resistance

    Nature Communications 11:3105.

    https://doi.org/10.1038/s41467-020-16932-z

    • PubMed
    • Google Scholar
70. 1. Luria SE
    2. Delbrück M

    (1943) Mutations of Bacteria from virus sensitivity to virus resistance

    Genetics 28:491–511.

    https://doi.org/10.1093/genetics/28.6.491

    • PubMed
    • Google Scholar
71. 1. Maltas J
    2. Krasnick B
    3. Wood KB

    (2020) Using selection by nonantibiotic stressors to sensitize Bacteria to antibiotics

    Molecular Biology and Evolution 37:1394–1406.

    https://doi.org/10.1093/molbev/msz303

    • PubMed
    • Google Scholar
72. 1. Maltas J
    2. Wood KB

    (2019) Pervasive and diverse collateral sensitivity profiles inform optimal strategies to limit antibiotic resistance

    PLOS Biology 17:e3000515.

    https://doi.org/10.1371/journal.pbio.3000515

    • PubMed
    • Google Scholar
73. 1. Meredith HR
    2. Lopatkin AJ
    3. Anderson DJ
    4. You L

    (2015a) Bacterial temporal dynamics enable optimal design of antibiotic treatment

    PLOS Computational Biology 11:e1004201.

    https://doi.org/10.1371/journal.pcbi.1004201

    • PubMed
    • Google Scholar
74. 1. Meredith HR
    2. Srimani JK
    3. Lee AJ
    4. Lopatkin AJ
    5. You L

    (2015b) Collective antibiotic tolerance: mechanisms, dynamics and intervention

    Nature Chemical Biology 11:182–188.

    https://doi.org/10.1038/nchembio.1754

    • PubMed
    • Google Scholar
75. 1. Michel JB
    2. Yeh PJ
    3. Chait R
    4. Moellering RC
    5. Kishony R

    (2008) Drug interactions modulate the potential for evolution of resistance

    PNAS 105:14918–14923.

    https://doi.org/10.1073/pnas.0800944105

    • PubMed
    • Google Scholar
76. 1. Moreno-Gamez S
    2. Hill AL
    3. Rosenbloom DI
    4. Petrov DA
    5. Nowak MA
    6. Pennings PS

    (2015) Imperfect drug penetration leads to spatial monotherapy and rapid evolution of multidrug resistance

    PNAS 112:E2874–E2883.

    https://doi.org/10.1073/pnas.1424184112

    • PubMed
    • Google Scholar
77. 1. Munck C
    2. Gumpert HK
    3. Wallin AI
    4. Wang HH
    5. Sommer MO

    (2014) Prediction of resistance development against drug combinations by collateral responses to component drugs

    Science Translational Medicine 6:262ra156.

    https://doi.org/10.1126/scitranslmed.3009940

    • PubMed
    • Google Scholar
78. 1. Nichol D
    2. Jeavons P
    3. Fletcher AG
    4. Bonomo RA
    5. Maini PK
    6. Paul JL
    7. Gatenby RA
    8. Anderson AR
    9. Scott JG

    (2015) Steering evolution with sequential therapy to prevent the emergence of bacterial antibiotic resistance

    PLOS Computational Biology 11:e1004493.

    https://doi.org/10.1371/journal.pcbi.1004493

    • PubMed
    • Google Scholar
79. 1. Nichol D
    2. Rutter J
    3. Bryant C
    4. Hujer AM
    5. Lek S
    6. Adams MD
    7. Jeavons P
    8. Anderson ARA
    9. Bonomo RA
    10. Scott JG

    (2019) Antibiotic collateral sensitivity is contingent on the repeatability of evolution

    Nature Communications 10:334.

    https://doi.org/10.1038/s41467-018-08098-6

    • PubMed
    • Google Scholar
80. 1. Ogbunugafor CB
    2. Eppstein MJ

    (2017) Competition along trajectories governs adaptation rates towards antimicrobial resistance

    Nature Ecology & Evolution 1:0007.

    https://doi.org/10.1038/s41559-016-0007

    • Google Scholar
81. Book

    1. Otto SP
    2. Day T

    (2011) A Biologist's Guide to Mathematical Modeling in Ecology and Evolution

    Princeton University Press.

    https://doi.org/10.1111/j.1442-9993.2007.01856.x

    • Google Scholar
82. 1. Pál C
    2. Papp B
    3. Lázár V

    (2015) Collateral sensitivity of antibiotic-resistant microbes

    Trends in Microbiology 23:401–407.

    https://doi.org/10.1016/j.tim.2015.02.009

    • PubMed
    • Google Scholar
83. 1. Parker GA
    2. Smith JM

    (1990) Optimality theory in evolutionary biology

    Nature 348:27–33.

    https://doi.org/10.1038/348027a0

    • Google Scholar
84. 1. Pena-Miller R
    2. Laehnemann D
    3. Jansen G
    4. Fuentes-Hernandez A
    5. Rosenstiel P
    6. Schulenburg H
    7. Beardmore R

    (2013) When the most potent combination of antibiotics selects for the greatest bacterial load: the smile-frown transition

    PLOS Biology 11:e1001540.

    https://doi.org/10.1371/journal.pbio.1001540

    • PubMed
    • Google Scholar
85. 1. Podnecky NL
    2. Fredheim EGA
    3. Kloos J
    4. Sørum V
    5. Primicerio R
    6. Roberts AP
    7. Rozen DE
    8. Samuelsen Ø
    9. Johnsen PJ

    (2018) Conserved collateral antibiotic susceptibility networks in diverse clinical strains of Escherichia coli

    Nature Communications 9:3673.

    https://doi.org/10.1038/s41467-018-06143-y

    • PubMed
    • Google Scholar
86. Book

    1. Podolsky SH

    (2015) The Antibiotic Era: Reform, Resistance, and the Pursuit of a Rational Therapeutics

    Johns Hopkins University Press.

    https://doi.org/10.3201/eid2106.150212

    • Google Scholar
87. 1. Price GR

    (1970) Selection and covariance

    Nature 227:520–521.

    https://doi.org/10.1038/227520a0

    • PubMed
    • Google Scholar
88. 1. Price GR

    (1972) Extension of covariance selection mathematics

    Annals of Human Genetics 35:485–490.

    https://doi.org/10.1111/j.1469-1809.1957.tb01874.x

    • PubMed
    • Google Scholar
89. 1. Read AF
    2. Day T
    3. Huijben S

    (2011) The evolution of drug resistance and the curious orthodoxy of aggressive chemotherapy

    PNAS 108 Suppl 2:10871–10877.

    https://doi.org/10.1073/pnas.1100299108

    • PubMed
    • Google Scholar
90. 1. Rodriguez de Evgrafov M
    2. Gumpert H
    3. Munck C
    4. Thomsen TT
    5. Sommer MO

    (2015) Collateral resistance and sensitivity modulate evolution of High-Level resistance to drug combination treatment in Staphylococcus aureus

    Molecular Biology and Evolution 32:1175–1185.

    https://doi.org/10.1093/molbev/msv006

    • PubMed
    • Google Scholar
91. 1. Roemhild R
    2. Gokhale CS
    3. Dirksen P
    4. Blake C
    5. Rosenstiel P
    6. Traulsen A
    7. Andersson DI
    8. Schulenburg H

    (2018) Cellular hysteresis as a principle to maximize the efficacy of antibiotic therapy

    PNAS 115:9767–9772.

    https://doi.org/10.1073/pnas.1810004115

    • PubMed
    • Google Scholar
92. 1. Roemhild R
    2. Linkevicius M
    3. Andersson DI

    (2020) Molecular mechanisms of collateral sensitivity to the antibiotic nitrofurantoin

    PLOS Biology 18:e3000612.

    https://doi.org/10.1371/journal.pbio.3000612

    • PubMed
    • Google Scholar
93. 1. Rosenkilde CEH
    2. Munck C
    3. Porse A
    4. Linkevicius M
    5. Andersson DI
    6. Sommer MOA

    (2019) Collateral sensitivity constrains resistance evolution of the CTX-M-15 β-lactamase

    Nature Communications 10:1–10.

    https://doi.org/10.1038/s41467-019-08529-y

    • PubMed
    • Google Scholar
94. 1. Russ D
    2. Kishony R

    (2018) Additivity of inhibitory effects in multidrug combinations

    Nature Microbiology 3:1339–1345.

    https://doi.org/10.1038/s41564-018-0252-1

    • PubMed
    • Google Scholar
95. 1. Santos-Lopez A
    2. Marshall CW
    3. Scribner MR
    4. Snyder DJ
    5. Cooper VS

    (2019) Evolutionary pathways to antibiotic resistance are dependent upon environmental structure and bacterial lifestyle

    eLife 8:e47612.

    https://doi.org/10.7554/eLife.47612

    • PubMed
    • Google Scholar
96. 1. Shoval O
    2. Sheftel H
    3. Shinar G
    4. Hart Y
    5. Ramote O
    6. Mayo A
    7. Dekel E
    8. Kavanagh K
    9. Alon U

    (2012) Evolutionary trade-offs, pareto optimality, and the geometry of phenotype space

    Science 336:1157–1160.

    https://doi.org/10.1126/science.1217405

    • PubMed
    • Google Scholar
97. Book

    1. Smith JM

    (1982) Evolution and the Theory of Games

    Cambridge University Press.

    https://doi.org/10.1017/CBO9780511806292

    • Google Scholar
98. 1. Sorg RA
    2. Lin L
    3. van Doorn GS
    4. Sorg M
    5. Olson J
    6. Nizet V
    7. Veening JW

    (2016) Collective resistance in microbial communities by intracellular antibiotic deactivation

    PLOS Biology 14:e2000631.

    https://doi.org/10.1371/journal.pbio.2000631

    • PubMed
    • Google Scholar
99. 1. Tan C
    2. Smith RP
    3. Srimani JK
    4. Riccione KA
    5. Prasada S
    6. Kuehn M
    7. You L

    (2012) The inoculum effect and band-pass bacterial response to periodic antibiotic treatment

    Molecular Systems Biology 8:617.

    https://doi.org/10.1038/msb.2012.49

    • PubMed
    • Google Scholar
100. 1. Taylor PD

     (1996) The selection differential in quantitative genetics and ess models

     Evolution 50:2106–2110.

     https://doi.org/10.1111/j.1558-5646.1996.tb03598.x

     • Google Scholar
101. 1. Tekin E
     2. Beppler C
     3. White C
     4. Mao Z
     5. Savage VM
     6. Yeh PJ

     (2016) Enhanced identification of synergistic and antagonistic emergent interactions among three or more drugs

     Journal of the Royal Society Interface 13:20160332.

     https://doi.org/10.1098/rsif.2016.0332

     • Google Scholar
102. 1. Tekin E
     2. Savage VM
     3. Yeh PJ

     (2017) Measuring higher-order drug interactions: a review of recent approaches

     Current Opinion in Systems Biology 4:16–23.

     https://doi.org/10.1016/j.coisb.2017.05.015

     • Google Scholar
103. 1. Tekin E
     2. Yeh PJ
     3. Savage VM

     (2018) General form for interaction measures and framework for deriving Higher-Order emergent effects

     Frontiers in Ecology and Evolution 6:166.

     https://doi.org/10.3389/fevo.2018.00166

     • Google Scholar
104. 1. Torella JP
     2. Chait R
     3. Kishony R

     (2010) Optimal drug synergy in antimicrobial treatments

     PLOS Computational Biology 6:e1000796.

     https://doi.org/10.1371/journal.pcbi.1000796

     • PubMed
     • Google Scholar
105. 1. Trindade S
     2. Sousa A
     3. Xavier KB
     4. Dionisio F
     5. Ferreira MG
     6. Gordo I

     (2009) Positive epistasis drives the acquisition of multidrug resistance

     PLOS Genetics 5:e1000578.

     https://doi.org/10.1371/journal.pgen.1000578

     • PubMed
     • Google Scholar
106. Preprint

     1. Vasse M
     2. Bonhoeffer S
     3. Frenoy A

     (2020) Ecological effects of stress drive bacterial evolvability under sub-inhibitory antibiotic treatments

     bioRxiv.

     https://doi.org/10.1101/2020.06.30.181099

     • Google Scholar
107. 1. Waxman D
     2. Gavrilets S

     (2005) Issues of terminology, gradient dynamics and the ease of sympatric speciation in adaptive dynamics

     Journal of Evolutionary Biology 18:1214–1219.

     https://doi.org/10.1111/j.1420-9101.2005.00953.x

     • PubMed
     • Google Scholar
108. 1. Wood K
     2. Nishida S
     3. Sontag ED
     4. Cluzel P

     (2012) Mechanism-independent method for predicting response to multidrug combinations in Bacteria

     PNAS 109:12254–12259.

     https://doi.org/10.1073/pnas.1201281109

     • PubMed
     • Google Scholar
109. 1. Wood KB
     2. Wood KC
     3. Nishida S
     4. Cluzel P

     (2014) Uncovering scaling laws to infer multidrug response of resistant microbes and Cancer cells

     Cell Reports 6:1073–1084.

     https://doi.org/10.1016/j.celrep.2014.02.007

     • PubMed
     • Google Scholar
110. 1. Wood KB
     2. Cluzel P

     (2012) Trade-offs between drug toxicity and benefit in the multi-antibiotic resistance system underlie optimal growth of E. coli

     BMC Systems Biology 6:48.

     https://doi.org/10.1186/1752-0509-6-48

     • PubMed
     • Google Scholar
111. 1. Woods RJ
     2. Read AF

     (2015) Clinical management of resistance evolution in a bacterial infection: a case study

     Evolution, Medicine, and Public Health 2015:281–288.

     https://doi.org/10.1093/emph/eov025

     • PubMed
     • Google Scholar
112. 1. Yoshida M
     2. Reyes SG
     3. Tsuda S
     4. Horinouchi T
     5. Furusawa C
     6. Cronin L

     (2017) Time-programmable drug dosing allows the manipulation, suppression and reversal of antibiotic drug resistance in vitro

     Nature Communications 8:15589.

     https://doi.org/10.1038/ncomms15589

     • PubMed
     • Google Scholar
113. 1. Yurtsev EA
     2. Chao HX
     3. Datta MS
     4. Artemova T
     5. Gore J

     (2013) Bacterial cheating drives the population dynamics of cooperative antibiotic resistance plasmids

     Molecular Systems Biology 9:683.

     https://doi.org/10.1038/msb.2013.39

     • PubMed
     • Google Scholar
114. 1. Yurtsev EA
     2. Conwill A
     3. Gore J

     (2016) Oscillatory dynamics in a bacterial cross-protection mutualism

     PNAS 113:6236–6241.

     https://doi.org/10.1073/pnas.1523317113

     • PubMed
     • Google Scholar
115. 1. Zhang Q
     2. Lambert G
     3. Liao D
     4. Kim H
     5. Robin K
     6. Tung CK
     7. Pourmand N
     8. Austin RH

     (2011) Acceleration of emergence of bacterial antibiotic resistance in connected microenvironments

     Science 333:1764–1767.

     https://doi.org/10.1126/science.1208747

     • PubMed
     • Google Scholar
116. 1. Zimmer A
     2. Katzir I
     3. Dekel E
     4. Mayo AE
     5. Alon U

     (2016) Prediction of multidimensional drug dose responses based on measurements of drug pairs

     PNAS 113:10442–10447.

     https://doi.org/10.1073/pnas.1606301113

     • PubMed
     • Google Scholar
117. 1. Zimmer A
     2. Tendler A
     3. Katzir I
     4. Mayo A
     5. Alon U

     (2017) Prediction of drug cocktail effects when the number of measurements is limited

     PLOS Biology 15:e2002518.

     https://doi.org/10.1371/journal.pbio.2002518

     • PubMed
     • Google Scholar

Author details

1. Erida Gjini

   Center for Computational and Stochastic Mathematics, Instituto Superior Tecnico, University of Lisbon, Portugal, Lisbon, Portugal

   Contribution

   Conceptualization, Formal analysis, Investigation, Methodology, Writing - original draft, Writing - review and editing

   For correspondence

   erida.gjini@tecnico.ulisboa.pt

   Competing interests

   No competing interests declared

   "This ORCID iD identifies the author of this article:" 0000-0002-0493-3102

2. Kevin B Wood

   Departments of Biophysics and Physics, University of Michigan, Ann Arbor, United States

   Contribution

   Conceptualization, Formal analysis, Investigation, Methodology, Writing - original draft, Writing - review and editing

   For correspondence

   kbwood@umich.edu

   Competing interests

   No competing interests declared

   "This ORCID iD identifies the author of this article:" 0000-0002-0985-7401

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