Acid-sensing ion channels (ASICs) are trimeric cation-selective channels activated by decreases in extracellular pH. The intracellular N and C terminal tails of ASIC1 influence channel gating, trafficking, and signaling in ischemic cell death. Despite several x-ray and cryo-EM structures of the extracellular and transmembrane segments of ASIC1, these important intracellular tails remain unresolved. Here we describe the coarse topography of the chicken ASIC1 intracellular domains determined by FRET, measured using either fluorescent lifetime imaging or patch clamp fluorometry. We find the C terminal tail projects into the cytosol by approximately 35 Å and that the N and C tail from the same subunits are closer than adjacent subunits. Using pH-insensitive fluorescent proteins, we fail to detect any relative movement between the N and C tails upon extracellular acidification but do observe axial motions of the membrane proximal segments towards the plasma membrane. Taken together, our study furnishes a coarse topographic map of the ASIC intracellular domains while providing directionality and context to intracellular conformational changes induced by extracellular acidification.
All analyzed results contributing to this study are included in the manuscript and supporting files. Source data files have been provided for all figures containing data.
- David M Maclean
- Tyler Couch
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
- Leon D Islas, Universidad Nacional Autónoma de México, Mexico
- Received: March 30, 2021
- Accepted: July 21, 2021
- Accepted Manuscript published: July 22, 2021 (version 1)
© 2021, Couch et al.
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