1. Biochemistry and Chemical Biology
  2. Developmental Biology

O-GlcNAc glycosylation orchestrates fate decision and niche function of bone marrow stromal progenitors

  1. Zengdi Zhang
  2. Zan Huang
  3. Mohamed Awad
  4. Mohammed Elsalanty
  5. James Cray
  6. Lauren E Ball
  7. Jason C Maynard
  8. Alma L Burlingame
  9. Hu Zeng
  10. Kim C Mansky
  11. Hai-Bin Ruan  Is a corresponding author
  1. Department of Integrative Biology and Physiology, University of Minnesota Medical School, United States
  2. Laboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, China
  3. National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, China
  4. Department of Medical Anatomical Sciences, College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, United States
  5. Department of Biomedical Education and Anatomy, The Ohio State University College of Medicine, and Division of Biosciences, The Ohio State University College of Dentistry, United States
  6. Department of Cell and Molecular Pharmacology & Experimental Therapeutics, Medical University of South Carolina, United States
  7. Department of Pharmaceutical Chemistry, University of California, San Francisco, United States
  8. Division of Rheumatology, Department of Internal Medicine, Mayo Clinic, United States
  9. Department of Immunology, Mayo Clinic, United States
  10. Department of Developmental and Surgical Sciences, School of Dentistry, University of Minnesota, United States
  11. Center for Immunology, University of Minnesota Medical School, United States
https://doi.org/10.7554/eLife.85464
Share this article
Cite this article
  1. Zengdi Zhang
  2. Zan Huang
  3. Mohamed Awad
  4. Mohammed Elsalanty
  5. James Cray
  6. Lauren E Ball
  7. Jason C Maynard
  8. Alma L Burlingame
  9. Hu Zeng
  10. Kim C Mansky
  11. Hai-Bin Ruan
(2023)
O-GlcNAc glycosylation orchestrates fate decision and niche function of bone marrow stromal progenitors
eLife 12:e85464.
https://doi.org/10.7554/eLife.85464
  2. Download BibTeX
  3. Download .RIS

Abstract

In mammals, interactions between the bone marrow (BM) stroma and hematopoietic progenitors contribute to bone-BM homeostasis. Perinatal bone growth and ossification provide a microenvironment for the transition to definitive hematopoiesis; however, mechanisms and interactions orchestrating the development of skeletal and hematopoietic systems remain largely unknown. Here, we establish intracellular O-linked β-N-acetylglucosamine (O-GlcNAc) modification as a posttranslational switch that dictates the differentiation fate and niche function of early BM stromal cells (BMSCs). By modifying and activating RUNX2, O-GlcNAcylation promotes osteogenic differentiation of BMSCs and stromal IL-7 expression to support lymphopoiesis. In contrast, C/EBPβ-dependent marrow adipogenesis and expression of myelopoietic stem cell factor (SCF) is inhibited by O-GlcNAcylation. Ablating O-GlcNAc transferase (OGT) in BMSCs leads to impaired bone formation, increased marrow adiposity, as well as defective B-cell lymphopoiesis and myeloid overproduction in mice. Thus, the balance of osteogenic and adipogenic differentiation of BMSCs is determined by reciprocal O-GlcNAc regulation of transcription factors, which simultaneously shapes the hematopoietic niche.

Editor's evaluation

Bone marrow stromal cells (BMSCs) can differentiate into a variety of cell types such as osteoblasts, chondrocytes, and adipocytes. The authors of this important study provide compelling and strong evidence that ablating O-GlcNAc transferase (OGT) in BMSCs impairs bone formation but promotes marrow adiposity. The results show that the balance of osteogenic and adipogenic differentiation of BMSCs is controlled by reciprocal O-GlcNAc regulation of lineage-specifying transcription factors, and highlights the importance of an intracellular glycosylation process of specific proteins in establishing the BM niche for hematopoiesis.

https://doi.org/10.7554/eLife.85464.sa0

Introduction

Mammalian bones support body structure, protect vital organs, and allow body movement. In addition, they provide an environment for hematopoiesis in the bone marrow (BM). Most bones in mammals are formed through endochondral ossification, which is initiated by mesenchymal condensation, followed by the differentiation of chondrocytes and perichondrial progenitors (Kobayashi and Kronenberg, 2021). Perichondrial progenitors expressing Osterix (encoded by the Sp7 gene) co-migrate with blood vessels into the primary ossification center, giving rise to osteoblasts and transient stromal cells in the nascent BM cavity (Chen et al., 2014; Liu et al., 2013; Maes et al., 2010; Mizoguchi et al., 2014). At the perinatal stage, Osterix+ progenitors contribute to osteo-lineages and long-lived BM stromal cells (BMSCs) that exhibit trilineage differentiation potential into osteocytes, chondrocytes, and adipocytes.

The decision of BMSC fate is controlled by a transcriptional network of pro-osteogenic and anti-adipogenic transcription factors that pre-establishes osteogenic enhancers in BMSCs for rapid bone formation (Rauch et al., 2019). RUNX family transcription factor 2 (RUNX2), by regulating osteogenic genes including Sp7, determines the osteoblast lineage from the multipotent BMSCs. Mice with Runx2 mutations completely lack skeletal ossification and die of respiratory failure (Komori et al., 1997). Runx2-haploinsufficient mice show specific skeletal abnormalities characteristic of human cleidocranial dysplasia (CCD), including persistent fontanels, delayed closure of cranial sutures, rudimentary clavicles, and dental abnormalities (Otto et al., 1997; Takarada et al., 2016). On the other hand, adipogenesis is driven by downregulation of pro-osteogenic factors, remodeling of the chromatin, and activation of adipogenic transcription factors, such as C/EBPs and PPARγ (Aaron et al., 2021; Rauch et al., 2019). BM adiposity is associated with bone loss in osteoporosis caused by aging, menopause, and anorexia nervosa (Bethel et al., 2013; Fazeli et al., 2013; Liu et al., 2015; Scheller et al., 2016). However, it is incompletely understood how these distinct types of transcription factors act cooperatively to determine lineage differentiation during neonatal skeletal development.

BMSCs and their lineage-differentiated progeny (e.g. osteoblasts and adipocytes) provide a niche microenvironment for hematopoiesis (Bianco and Robey, 2015; Calvi and Link, 2015; Morrison and Scadden, 2014; Wei and Frenette, 2018). Recent studies using single-cell technologies and lineage tracing experiments have started to unveil the complexity and heterogeneity of niche cell types, niche factors, and their actions. For example, BMSC-derived stem cell factor (SCF, encoded by the Kitl gene) and CXC chemokine ligand 12 (CXCL12) are required for the maintenance and differentiation of hematopoietic stem/progenitor cells (HSPCs) (Asada et al., 2017; Ding et al., 2012). A prominent subpopulation of perivascular BMSCs express adipocyte markers (Dolgalev and Tikhonova, 2021; Zhong et al., 2020; Zhou et al., 2017), and support steady-state and metabolic-stressed myelopoiesis by secreting SCF (Zhang et al., 2019). Meanwhile, osteolineage cells are crucial for lymphopoiesis (Wei and Frenette, 2018). Depleting Osterix+ cells halts B cell maturation and causes immune failure (Yu et al., 2016). IL-7, the most crucial factor for lymphoid progenitors, is expressed by a subset of BMSCs (Fistonich et al., 2018). While it is well accepted that myeloid and lymphoid progenitors may reside in distinct BM niches, it is unclear how BMSC heterogeneity is established during early development and whether cytokine expression is coordinated and controlled by the fate-defining transcriptional network in BMSCs.

Post-translational modifications (PTMs), including phosphorylation, acetylation, and ubiquitination, allow the precise regulation of stability, localization, and activity of BM transcriptional factors, such as RUNX2 (Chen et al., 2021; Kim et al., 2020), C/EBPs (Wang et al., 2022), and PPARγ (Brunmeir and Xu, 2018). It remains poorly defined how these modifications are coordinated in a spatio-temporal manner to calibrate skeletal development. Thousands of intracellular proteins are dynamically modified by a single O-linked N-Acetylglucosamine (O-GlcNAc) moiety at serine or threonine residues, termed O-GlcNAcylation (Hart et al., 2007; Ruan et al., 2013b; Yang and Qian, 2017). O-GlcNAc transferase (OGT), using UDP-GlcNAc derived from the hexosamine biosynthetic pathway as the substrate, controls diverse biological processes such as gene transcription, protein stability, and cell signaling (Hanover et al., 2012; Ruan et al., 2014; Ruan et al., 2012; Ruan et al., 2013a). In cell culture, O-GlcNAcylation promotes osteogenesis (Kim et al., 2007; Nagel and Ball, 2014) and suppresses adipogenesis (Ji et al., 2012). However, the physiological relevance of O-GlcNAcylation in skeletal development and remodeling has not been established. Here, we studied OGT in balancing osteogenic versus adipogenic programs and in controlling niche function of BMSC in mice. The multifaceted role of protein O-GlcNAcylation is achieved through reciprocal regulation of pro-osteogenic, pro-lymphopoietic RUNX2 and pro-adipogenic, pro-myelopoietic C/EBPβ.

Results

Loss of OGT in perinatal BMSCs leads to bone loss

To determine the in vivo role of protein O-GlcNAcylation in bone development, we deleted the X Chromosome-located Ogt gene using the Sp7GFP:Cre mice (Figure 1A). Floxed Ogtfl/fl were bred with Sp7GFP:Cre to generate Ogt conditional knockout (cKO) mice. Compared to Sp7GFP:Cre littermate controls, newborn Ogt cKO mice showed no obvious change in long bone formation (Figure 1B) but had a profound defect in the mineralization of flat bones of the calvaria (Figure 1C), suggesting impaired intramembranous ossification during the prenatal stage.

Figure 1 with 1 supplement see all
Download asset Open asset
Impaired osteogenesis in Ogt cKO mice.

(A) Mating strategy to generate Ogt cKO mice. Note that the Ogt gene is located on Chr. X, thus males are hemizygous Ogtfl/Y. (B, C) Whole mount Alizarin red and Alcian blue staining of newborn mice. (D, E) Long bone length (D) and gross morphology of 4–6 weeks old mice. (F, G) Goldner’s trichrome (F) and Safranin O (G) staining of femurs from 4-week-old mice. (H–L) Micro-CT of 6-week-old mice (H, n=3–4). Bone volume (BV, I), BV/tissue volume ratio (BV/TV, J), trabecular thickness (Tb.Th, K), and trabecular number (Tb.N, L) were calculated. Data are presented as mean ± SEM. *, p<0.05 by unpaired student’s t-test.

At 4–6 weeks of age, Ogt cKO mice were modestly shorter than the controls (Figure 1D and E). Histological analyses showed decreased bone volume and osteoblast number (Figure 1F) and shortened growth plate (Figure 1G) in Ogt cKO mice. Micro-CT scanning further showed that Ogt cKO mice had reduced trabecular bone volume, bone volume to tissue volume ratio, trabecular thickness, and trabecular numbers in the distal femur (Figure 1H–L). Ogt cKO mice represent typical bone and dental defects (Figure 1—figure supplement 1) as observed in Runx2-haploinsufficient mice (Otto et al., 1997; Takarada et al., 2016), suggesting that O-GlcNAcylation might control RUNX2 function. Moist food was provided to these animals after weaning to prevent malnutrition.

RUNX2 O-GlcNAcylation promotes osteogenesis

To investigate how OGT controls osteogenic differentiation of BMSCs, we first isolated primary BMSCs from control and Ogt cKO mice and induced them into osteoblast cells. Alkaline phosphatase staining revealed a reduction in mineralization of Ogt cKO BMSCs (Figure 2A). Similarly, treating mesenchymal C3H10T1/2 cells with an OGT inhibitor, OSMI-1, reduced mineralization (Figure 2B) and ablated calcium deposition (Figure 2C) after osteogenic differentiation. Parathyroid hormone (PTH) is a bone anabolic agent that requires RUNX2-dependent signaling (Krishnan et al., 2003). We found that PTH treatment of C3H10T1/2 cells increased global protein O-GlcNAcylation (Figure 2D). The ability of PTH to activate osteogenesis is completely abolished when OGT was inhibited by OSMI-1 (Figure 2E). Pharmacological activation of O-GlcNAcylation enhances RUNX2 activity and promotes osteogenic differentiation (Kim et al., 2007; Nagel and Ball, 2014). We mutated three known O-GlcNAc sites on RUNX2, Ser 32 and Ser 33 in the N-terminal transactivation domain and Ser 371 in the proline/serine/threonine-rich domain (Figure 2F), to alanine (3A), and found that mutant RUNX2 possessed less O-GlcNAcylation (Figure 2G). O-GlcNAcase (OGA) inhibition by Thiamet-G (TMG) increased O-GlcNAcylation of wildtype (WT) RUNX2, but to a much less extent in the 3A mutant (Figure 2G). OGT inhibition by OSMI-1 or O-GlcNAc mutation both impaired the transcriptional activity of RUNX2 on a luciferase reporter (Figure 2H). OSMI-1 could still suppress of luciferase activity of RUNX2-3A (Figure 2H), suggesting additional, unidentified O-GlcNAc sites (Figure 2G), which requires future investigation. Nevertheless, when overexpressed in C3H10T1/2 cells, RUNX2-3A substantially lost the ability to induce osteogenic differentiation (Figure 2I) or RUNX2-target gene expression (Figure 2J). These data demonstrate that O-GlcNAcylation is essential for RUNX2 activity and osteogenesis.

Figure 2
Download asset Open asset
RUNX2 O-GlcNAcylation is required for osteogenesis.

(A) Alkaline phosphatase (ALP) staining of control and Ogt cKO BMSCs differentiated to the osteogenic lineage. (B, C) Primary BMSCs, in the presence or absence of the OGT inhibitor OSMI-1, were induced for osteogenesis and stained for ALP (B) and Alizarin Red S (C). (D) Primary BMSCs were treated with PTH for the indicated time and subjected to Western blotting of total protein O-GlcNAcylation. (E) BMSCs were treated with PTH alone or together with OSMI-1, osteogenic differentiated, and stained for ALP (n=3). (G) Flag-tagged wildtype (WT) and O-GlcNAc mutant (3A) RUNX2 plasmids were overexpressed in HEK293 cells, and their O-GlcNAcylation was determined by Flag immunoprecipitation followed with O-GlcNAc western blot. (H) 6xOSE-luciferase activity in COS-7 cells transfected with WT or 3A-mutant RUNX2, in the presence or absence of the OGT inhibitor, OSMI-1. (I, J) C3H10T1/2 cells with lentiviral overexpression of RUNX2 were osteogenically differentiated and stained with ALP or Alizarin Red S (I). Expression of Bglap and Rankl was determined by RT-qPCR (J). Data are presented as mean ± SEM. *, p<0.05; **, p<0.01; and ***, p<0.001 by two-way ANOVA (H) or one-way ANOVA (J). Representative images from at least three biological replicates were shown in A, B, C, E, and I.

Figure 2—source data 1

Raw uncropped images for panel D.

https://cdn.elifesciences.org/articles/85464/elife-85464-fig2-data1-v2.zip
Figure 2—source data 2

Raw uncropped images for panel G.

https://cdn.elifesciences.org/articles/85464/elife-85464-fig2-data2-v2.zip

In adult mice, Sp7 expression is restricted to osteoblast precursors. We treated Ogt cKO mice from pregnancy with doxycycline (Dox) and withdrew Dox at 10 weeks of age to induce Cre expression and OGT depletion only during adulthood (Figure 3A). Micro-CT showed that Ogt cKO mice had reduced bone volume, trabecular thickness, and bone mineral density (Figure 3B–E). Together, these results support the functional indispensability of OGT in the committed osteolineage for adult trabecular bone remodeling.

Figure 3
Download asset Open asset
Adult-onset deletion of OGT impairs trabecular bone formation.

(A) Dox treatment timeline in Ogt cKO to achieve osteoblast-specific deletion of OGT. (B–D) Micro-CT (B) showing reduced bone volume/tissue volume (C), trabecular thickness (D), and trabecular bone mineral density (E). Data are presented as mean ± SEM.*, p<0.05 by unpaired student’s t-test.

C/EBPβ O-GlcNAcylation inhibits the adipogenic specification of BMSCs

The osteogenic and adipogenic differentiation of BMSCs is generally considered mutually exclusive (Ambrosi et al., 2017). Concomitant with bone loss, we observed a massive accumulation of adipocytes in the bone marrow of Ogt cKO mice, shown by hematoxylin & eosin staining (Figure 4A) and immune-staining of the lipid droplet protein – perilipin (Figure 4B). Pdgfrα and Vcam1 (encoding CD106) have been recently identified as surface markers of adipogenic lineage cells in the BM that also express the Lepr and Adipoq genes (Figure 4—figure supplement 1; Baryawno et al., 2019; Zhong et al., 2020). Flow cytometric analysis of BMSCs revealed that Ogt cKO mice possessed more PDGFRα+VCAM1+ adipogenic progenitors than littermate controls (Figure 4C). To directly test if OGT deficiency biases BMSC differentiation toward the adipogenic lineage, we first induced the adipogenic differentiation of primary BMSCs and found increased lipid deposition in Ogt cKO mice (Figure 4D). Even under an osteogenic induction condition, adipo-lineage markers such as Adipoq and Vcam1 were significantly upregulated by OGT deficiency (Figure 4E and F). Furthermore, treating C3H10T1/2 mesenchymal cells with an OGA inhibitor TMG to increase protein O-GlcNAcylation, was able to substantially reduce perilipin protein expression (Figure 4G) and Pparg and Adipoq gene expression (Figure 4H, I). These data indicate that OGT inhibits the adipogenic program of BMSCs.

Figure 4 with 3 supplements see all
Download asset Open asset
O-GlcNAcylation inhibits BM adipogenesis.

(A, B) H&E (A) and Perilipin immunofluorescent staining (B) on femur sections from 4-week-old mice. (C) Flow cytometric quantification of PDGFRa+VCAM1+ preadipocytes frequencies within the live BM cells (n=3). (D) Adipogenic differentiation of primary BMSCs from control and Ogt cKO mice. Lipid was stained with Oil Red O and quantified to the right (n=4). (E, F) Primary BMSCs were osteogenic differentiated for 0 or 15 days. Expression of Adipoq (E) and Vcam1 (F) genes was determined by RT-qPCR (n=3). (G–I) C3H10T1/2 cells, treated with or without TMG, were adipogenic differentiated. Western blotting for perilipin and O-GlcNAc of differentiated cells (G) and RT-qPCR for adipogenic marker Pparg (H) and Adipoq (I) expression. (J–M) Adipogenic differentiation of C3H10T1/2 cells infected with lentiviral C/EBPβ. Oil Red O was stained (J) and quantified (K). Pparg (L) and Adipoq (M) gene expression was determined by RT-qPCR. Data are presented as mean ± SEM.*, p<0.05; **, p<0.01; and ***, p<0.001 by unpaired student’s t-test (C, D, K), one-way ANOVA (L, M), and two-way ANOVA (E, F, H, I).

Figure 4—source data 1

Mass spectrometry search results of all protein modifications (Table S1) and PPARγ2 O-GlcNAc sites (Table S2).

https://cdn.elifesciences.org/articles/85464/elife-85464-fig4-data1-v2.zip
Figure 4—source data 2

Raw uncropped images for panel G.

https://cdn.elifesciences.org/articles/85464/elife-85464-fig4-data2-v2.zip

We went on to determine the O-GlcNAc targets of OGT in suppressing adipogenesis. As an osteogenic regulator, RUNX2 also reciprocally suppresses the adipogenic program (Ahrends et al., 2014). However, such suppression was not dependent on O-GlcNAcylation, because O-GlcNAc-deficient RUNX2 displayed similar efficiency as the wildtype protein to reduce lipid deposition and perilipin expression in differentiated C3H10T1/2 cells (Figure 4—figure supplement 2). It is possible that O-GlcNAc on RUNX2 selectively facilitates the recruitment of transcriptional co-activators for osteogenesis but does not suppress the chromatin remodeling needed for the activation of adipogenic transcriptional factors.

PPARγ1 is O-GlcNAcylated at T54 in the A/B activation domain (Ji et al., 2012), corresponding to T84 in PPARγ2, the major isoform in adipocytes (Figure 4—figure supplement 3A). Mutating T84 in PPARγ2 did not ablate the ability of the OGA inhibitor TMG to suppress adipogenesis in C3H10T1/2 cells (data not shown), suggesting the existence of other unidentified O-GlcNAc sites on PPARγ2 or other target proteins than PPARγ2. Through mass spectrometry, we were able to map four additional O-GlcNAc sites on PPARγ2 (Figure 4—figure supplement 3A and Figure 4—source data 1). Intriguingly, mutating these four sites or together with T84 to alanine, render PPARγ2 incompetent to induce transcription and adipogenesis (Figure 4—figure supplement 3B, C). It suggests that PPARγ2 O-GlcNAcylation is essential for adipocyte maturation, but likely does not mediate the anti-adipogenic effect of OGT in perinatal BMSCs.

We then looked to C/EBPβ, an early transcription factor that specifies the adipogenic fate of BMSCs (Cao et al., 1991; Darlington et al., 1998). It has been reported that OGT modifies C/EBPβ to inhibit its transcriptional activity (Li et al., 2009; Qian et al., 2018). As expected, ablating O-GlcNAcylation of C/EBPβ (2A mutation) promotes adipogenic differentiation of C3H10T1/2 cells (Figure 4J and K). Taken together, we concluded that, by O-GlcNAcylating and reciprocally regulating RUNX2 and C/EBPβ, OGT is required for the proper allocation of skeletal progenitors into osteogenic versus adipogenic lineages during development.

OGT deficiency disrupts the BM niche

Skeletal development is concomitant with the establishment of definitive hematopoiesis in the BM. To test if OGT deficiency affects the niche function of Sp7+ cells for B-cell lymphopoiesis, we performed flow cytometry analyses of bone marrow of 4-week-old mice (Figure 5A, Figure 5—figure supplement 1 and Figure 5—source data 1; Hardy et al., 1991). No changes in the percentage of lineage-Sca-1+Kit+ (LSK) progenitor cells, common lymphoid progenitors (CLPs), Fraction A that contains pre-pro-B cells were observed between control and Ogt cKO mice (Figure 5B–D). While frequencies of Fraction B and C pro-B, pre-B, and immature B in Ogt cKO mice were drastically reduced (Figure 5E–I), demonstrating a developmental blockage from pre-pro-B to pro-B cells. In the peripheral blood, there was specific loss of CD19+B220+ B cells but not CD4+ or CD8+ T cells (Figure 5J–L). B-cell dysfunction observed here was similar to the phenotype in mice when all Sp7+ cells were depleted (Yu et al., 2016) or IL-7 was deleted in BMSCs (Cordeiro Gomes et al., 2016), indicating that O-GlcNAcylation is essential for the Sp7+ lineage cells to establish a niche environment for B-cell lymphopoiesis.

Figure 5 with 1 supplement see all
Download asset Open asset
Impaired B lymphopoiesis and myeloid skewing in Ogt cKO mice.

(A) Schematic view of B cell development in the BM and blockade by stromal OGT deficiency (red X). (B–C) Flow cytometric quantification of LSK (B) and CLP (C) among live BM cells (n=4–6). (D–I) Flow cytometric quantification of fraction A (D), fraction B (E), fraction C (F), fraction C’ (G), fraction D (H), and immature B (I) frequencies among live BM lymphocytes (n=6–7). (J–L) Flow cytometric quantification of B220+ B cell (J), CD4+ T cell (K), and CD8+ T cell (L) percentages in the blood (n=3–4). (M, N) CMP/CLP ratio (M) and GMP/EMP ratio (N) in the BM (n=6–7). (O, P) Complete blood counting showing numbers of RBC (O) and neutrophil (P) (n=7–9). Data are presented as mean ± SEM. *, p<0.05; **, p<0.01; and ***, p<0.001 by unpaired student’s t-test.

Figure 5—source data 1

Antibodies used for flow cytometry.

https://cdn.elifesciences.org/articles/85464/elife-85464-fig5-data1-v2.zip

BM adiposity is associated with myeloid overproduction in conditions including aging, irradiation (Ho et al., 2019), osteopenia (Kajkenova et al., 1997), and obesity (Singer et al., 2014), indicating the supportive function of marrow adipocytes on demand-adapted myelopoiesis. Consistently with the increased BM adiposity found in Ogt cKO mice, we also observed biased HSPC differentiation toward the myeloid lineage, as shown by increased ratio of common myeloid progenitor (CMP) to common lymphoid progenitors (CLPs) and ratio of granulocyte-monocyte progenitors (GMP) to megakaryocyte-erythrocyte progenitors (MEP) in the BM (Figure 5M and N). As a result, increased numbers of red blood cells and neutrophils were observed in the blood of Ogt cKO mice (Figure 5O and P). Together, these results demonstrate that OGT deficiency in neonatal BMSCs establishes a BM environment that promotes myelopoiesis and simultaneously impairs B cell development.

Transcriptional regulation of niche cytokines by RUNX2 and C/EBPβ O-GlcNAcylation

BMSC-derived SCF (encoded by the Kitl gene) and IL-7 are required for the myeloid differentiation and B-cell development, respectively (Asada et al., 2017; Cordeiro Gomes et al., 2016; Ding et al., 2012). We sought to test if their expression is controlled by the same transcriptional network determining BMSC fate. Adipogenic differentiation of mesenchymal C3H10T1/2 cells concomitantly increased Kitl while decreased Il7 gene expression (Figure 6A and B). Simultaneous treatment with the OGT inhibitor OSMI-1 dampened Il7 expression before differentiation but enhanced Kitl expression in differentiated adipocytes (Figure 6A and B). On the other hand, osteogenic differentiation suppressed Kitl transcription, which could be further inhibited by TMG that elevated global O-GlcNAcylation (Figure 6C). While Il7 mRNA levels were not evidently affected by osteogenic differentiation, TMG stimulated its expression (Figure 6D). O-GlcNAcylation inhibits the adipogenesis specified by C/EBPβ but supports osteogenesis determined by RUNX2. In concert, C/EBPβ overexpression in C3H10T1/2 cells activated Kitl transcription and suppressed Il7 expression, which was further exacerbated by O-GlcNAc-deficient C/EBPβ (Figure 6E and F). However, RUNX2 overexpression decreased Kitl mRNA levels (Figure 6G). When compared to the wildtype, O-GlcNAc-defective RUNX2 was impaired in inducing Il7 expression (Figure 6H). Collectively, these results reveal that protein O-GlcNAcylation, by acting on BMSC lineage transcriptional factors, establishes a pro-lymphopoietic niche during neonatal bone development and at the same time prevents the myeloid-skewing, adipogenic BM environment.

Figure 6
Download asset Open asset
O-GlcNAc regulation of niche cytokine expression.

(A, B) C3H10T1/2 cells were treated with vehicle or OGT inhibitor OSMI and differentiated for adipocytes (n=4). Kitl (A) and Il7 (B) gene expression was determined by RT-qPCR. (C, D) C3H10T1/2 cells were treated with vehicle or OGA inhibitor TMG and induced for osteogenic differentiation (n=6). Kitl (C) and Il7 (D) gene expression was determined by RT-qPCR. (E–H) C3H10T1/2 cells were infected with lentiviruses expressing WT and O-GlcNAc-deficient C/EBPβ (E, F) or RUNX2 (G, H). Expression Kitl (E, G) and Il7 (F, H) was measured by RT-qPCR (n=6). (I) Proposed action of protein O-GlcNAcylation in regulating the BMSC niche function. Data are presented as mean ± SEM. *, p<0.05; **, p<0.01; ***, p<0.001 by two-way ANOVA (A–D) and one-way ANOVA (E–H).

Figure 6—source data 1

Sequences of oligos used for RT-qPCR.

https://cdn.elifesciences.org/articles/85464/elife-85464-fig6-data1-v2.zip

Discussion

Post-translational modification networks exist in the bone-BM organ to regulate its development and remodeling. Given that definitive hematopoiesis is matured in perinatal BM, it is tempting to hypothesize that the regulatory mechanisms guiding the development of bone also establish the BM niche for hematopoiesis. However, experimental evidence has been largely lacking so far. In the present study, we examined the vital role of the under-studied protein O-GlcNAcylation in determining the osteogenic versus adipogenic fate specification of BMSCs and in balancing the pro-lymphopoietic and pro-myelopoietic niche function of BMSCs. We showed that, by modifying and reciprocally regulating RUNX2 and C/EBPβ, O-GlcNAc orchestrates the early development of skeletal and hematopoietic systems (Figure 7).

Figure 7
Download asset Open asset
Working model of O-GlcNAc signaling in bone-BM development.

Multiple temporally and spatially distinct types of progenitors contribute to bone development and maintenance. In the early embryo, Sp7+ progenitors give rise to fetal bone tissues and transient stromal cells that disappear in early postnatal life (Mizoguchi et al., 2014). Perinatally, Sp7+ progenitors contribute to osteolineage cells and long-lived perivascular BMSCs that can be labeled by leptin receptor (Lepr) and adiponectin (Adipoq) (Zhong et al., 2020; Zhou et al., 2017). Recent evidence suggests that a significant portion of adult BMSCs and osteoblasts originate from collagen II (Col2)- and aggrecan (Acan)-expressing chondrocytes (Ono et al., 2014). Due to the fact that Sp7GFP:Cre targets osteoblasts, BMSCs, and a subset of chondrocytes (Chen et al., 2014; Liu et al., 2013), the current study could not delineate the exact developmental stages and the primary cellular compartments where OGT instructs bone development. Nonetheless, our ex vivo experiments and adult-onset targeting of OGT in Sp7+ osteoblasts, together with prior published in vivo and in vitro evidence (Andrés-Bergós et al., 2012; Nagel and Ball, 2014; Nagel et al., 2013), certainly reveal the indispensability of protein O-GlcNAcylation for chondro-osteogenic differentiation. While this study primarily focused early life bone development, it is warranted to further investigate the role of OGT in the transition to appositional remodeling during adulthood (Shu et al., 2021) and in osteoporosis pathogenesis during aging. Moreover, bone-forming skeletal stem cells (SSCs) are identified in other anatomical regions of long bones, such as growth plate, periosteum, and endosteum (Ambrosi et al., 2019). It remains undetermined whether O-GlcNAcylation is abundant in and controls the development and function of these SSC populations.

O-GlcNAcylation is required for PPARγ to drive adipogenesis, but why did not OGT-deficient BMSCs arrest their differentiation after being committed to the adipogenic lineage. One speculation is that PPARγ O-GlcNAcylation is extremely low in homeostatic conditions, which might help explain the rare appearance of adipocytes in young BM. If so, the loss of PPARγ O-GlcNAcylation in Ogt cKO mice would not block adipogenesis. Second, the differentiation of marrow adipose tissue (MAT) is distinct from peripheral white adipose tissue (WAT). For instance, adipogenic BMSCs in adult mice already express large amount of Adipoq, which is only present in mature WAT adipocytes. Certain forms of genetic lipodystrophy (e.g. mutations in CAV1 and PTRF) selectively lose peripheral WAT but preserve MAT (Scheller et al., 2015). These findings suggest that MAT might be less dependent on PPARγ or able to adopt alternative differentiation when PPARγ is absent or inhibited. In fact, a recent publication reported a secondary adipogenic pathway in lipodystrophic 'fat-free' mice (Zhang et al., 2021). Lastly, our preliminary examination of old Ogt cKO mice revealed the resolution of BM adiposity, indicating that PPARγ and its O-GlcNAc modification become essential for the adipogenic differentiation of adult BMSCs.

Protein O-GlcNAcylation senses glucose availability (Hardivillé and Hart, 2014; Ruan et al., 2012), hormonal cues (Ruan et al., 2014; Ruan et al., 2017; Whelan et al., 2008), cellular stress (Martinez et al., 2017; Ruan et al., 2017), and immune signals (Chang et al., 2020; Liu et al., 2019; Zhao et al., 2020; Zhao et al., 2022) to maintain cellular and tissue homeostasis. Osteogenic differentiation of mesenchymal cells induces global O-GlcNAc levels (Kim et al., 2007; Nagel and Ball, 2014); however, the upstream mechanistic regulators of osteoblastic O-GlcNAcylation remain enigmatic. High glucose has been shown to promote O-GlcNAcylation and osteogenic differentiation of cartilage endplate stem cells (Sun et al., 2019). BMSCs preferentially use glycolysis for bioenergetics to support their self-renewal and multipotency (Ito and Suda, 2014; van Gastel and Carmeliet, 2021). Active aerobic glycolysis also fuels the high anabolic demand during bone formation. It would be important in the future to determine whether flux of the hexosamine biosynthetic pathway, a branch of glycolysis (Ruan et al., 2013b), increases to provide more UDP-GlcNAc for O-GlcNAc modification. We also showed here that PTH treatment increased protein O-GlcNAcylation. Signaling through the PTH receptor activates the cAMP-protein kinase A (PKA)-CREB pathway and the accumulation of inositol trisphosphate (IP3) and diacylglycerol (DAG), which further increase intracellular Ca2+ and PKC, respectively (Datta and Abou-Samra, 2009). Future experiments are required to determine if OGT enzymatic activity can be regulated by these signaling nodes, for example Ca2+/calmodulin-dependent protein kinase II (CaMKII) (Ruan et al., 2017). Sex differences in skeletal development, maintenance, and aging have been well appreciated. Whether BMSC O-GlcNAc signaling is differentially regulated between male and female animals, particularly during puberty and aging, is an important question that remains unaddressed. Since only male mice were investigated in the current study, it is unclear if the reciprocal regulation of RUNX2 and PPARγ by O-GlcNAcylation in determining the bone-fat balance is equally vital in females.

The BM microenvironment, composed of BMSCs, osteoblasts, adipocytes, sympathetic nerves, and vascular endothelial cells, has been highlighted as an important extrinsic factor for the maintenance and differentiation of distinct hematopoietic lineage progenitors (Bianco and Robey, 2015; Calvi and Link, 2015; Morrison and Scadden, 2014; Wei and Frenette, 2018). While the concomitant development, remodeling, and aging of the skeletal and hematopoietic systems have been observed in various pathophysiological conditions, mechanisms underlying the coordinated regulation of the two systems are less understood. Our current study has provided the first evidence that RUNX2, permitted by O-GlcNAcylation, not only is indispensable for the osteoblast development, but also establishes the endosteal niche for B lymphocytes by driving IL-7 expression (Figure 7). When OGT is deficient, the perivascular BMSCs are prone to adipogenic differentiation, which also activates C/EBPβ-dependent SCF expression and myelopoiesis. During aging, the parallel dysfunction of the skeletal and hematopoietic systems leads to osteoporosis, marrow fat accumulation, and myeloid hematopoietic skewing (Geiger et al., 2013). Whether BMSC aging is associated with O-GlcNAc decline and whether the balance between RUNX and C/EBPβ leads to bone-fat imbalances and niche dysfunction require future investigations.

Methods

Key resources table
Reagent type (species) or resourceDesignationSource or referenceIdentifiersAdditional information
Genetic reagent
(Mus musculus)		Sp7GFP:Cre (B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J)Jackson LaboratoryRRID:IMSR_JAX:006361
Genetic reagent
(Mus musculus)		Ogtfl/fl (B6.129-Ogttm1Gwh/J)Jackson LaboratoryRRID:IMSR_JAX:004860
Cell line (Mus musculus)Primary BMSCThis paperFrom long bones of mouse
Cell line (Mus musculus)C3H10T1/2ATCCCCL-226Verified by ATCC and tested negative for mycoplasma
Cell line (Homo sapiens)HEK293FTInvitrogenR70007Verified by Invitrogen and tested negative for mycoplasma
Cell line (Cercopithecus aethiops)COS7ATCCCRL-1651Verified by ATCC and tested negative for mycoplasma
Transfected construct (Mus musculus)6xOSE2-lucPhimphilai et al., 2006
Transfected construct (Renilla reniformis)pGL4-hRlucPromega#E688A
Transfected construct (Mus musculus)PPREx3-TK-lucAddgene#1015
AntibodyAnti-Perilipin
(Rabbit monoclonal)		Cell Signaling Technology9349TIF(1:200)
AntibodyAnti-B220 (Rat monoclonal)Life Technologies67-0452-82FC(1:200)
AntibodyAnti-CD43
(Rat monoclonal)		BD Biosciences553271FC(1:200)
AntibodyAnti-CD24 (Rat monoclonal)Biolegend101822FC(1:1000)
AntibodyAnti-Ly-51 (Rat monoclonal)Biolegend108305FC(1:200)
AntibodyAnti-CD127 (Rat monoclonal)Tonbo Biosciences20–1271 U100FC(1:200)
AntibodyAnti-CD25 (Rat monoclonal)Life Technologies63-0251-82FC(1:200)
AntibodyAnti-CD19
(Rat monoclonal)		Biolegend115545FC(1:200)
AntibodyBiotin-conjugated lineage antibodies
(Rat monoclonal)		Biolegend133307FC(1:200)
AntibodyAnti-CD4
(Rat monoclonal)		Biolegend100403FC(1:200)
AntibodyAnti-CD5
(Rat monoclonal)		Biolegend100603FC(1:200)
AntibodyAnti-CD8
(Rat monoclonal)		Biolegend100703FC(1:200)
AntibodyAnti-CD127-APC
(Rat monoclonal)		eBioscience17-1271-82FC(1:100)
AntibodyAnti-c-Kit-APC-eFluor780
(Rat monoclonal)		eBioscience47-1171-82FC(1:400)
AntibodyAnti-Sca-1-Super Bright 436
(Rat monoclonal)		eBioscience62-5981-82FC(1:100)
AntibodyAnti-CD34-PE
(Rat monoclonal)		Biolegend152204FC(1:100)
AntibodyAnti-FcγR-PerCP-eFluor710
(Rat monoclonal)		eBioscience46-0161-80FC(1:400)
AntibodyAnti-CD150-BV605
(Rat monoclonal)		Biolegend115927FC(1:100)
AntibodyAnti-CD48-BUV395
(Rat monoclonal)		BD Biosciences740236FC(1:100)
AntibodyAnti-CD45-BUV395
(Rat monoclonal)		BD Biosciences564279FC(1:400)
AntibodyAnti-Ter119-BV421
(Rat monoclonal)		Biolegend116234FC(1:400)
AntibodyAnti-CD31-BV421
(Rat monoclonal)		Biolegend102424FC(1:400)
AntibodyAnti-PDGFRa-Super Bright 600
(Rat monoclonal)		eBioscience63-1401-82FC(1:100)
AntibodyAnti-VCAM1-PE
(Rat monoclonal)		Biolegend105713FC(1:100)
Recombinant DNA reagentRUNX2-WTThis paperpLV-EF1a-RUNX2-WT-IRES-HygroSee Methods; available upon request
Recombinant DNA reagentRUNX2-3A/ RUNX2-3SAThis paperpLV-EF1a-RUNX2-3Mut-IRES-HygroSee Methods; available upon request
Recombinant DNA reagentPPARλ2-WTThis paperpLVX- PPARλ2-WT-PuroSee Methods; available upon request
Recombinant DNA reagentPPARλ2-T84AThis paperpLVX- PPARλ2-T84A-PuroSee Methods; available upon request
Recombinant DNA reagentPPARλ2-4AThis paperpLVX- PPARλ2-4A-PuroSee Methods; available upon request
Recombinant DNA reagentPPARλ2-5AThis paperpLVX- PPARλ2-5A-PuroSee Methods; available upon request
Recombinant DNA reagentC/EBPβ-WTThis paperpCDH-CMV-Cebpb-WT-P2a-PuroSee Methods; available upon request
Recombinant DNA reagentC/EBPβ–2AThis paperpCDH-CMV-Cebpb-2MUT-P2a-PuroSee Methods; available upon request
Commercial assay or kitQ5 Site-Directed Mutagenesis KitNEB#E0554
Commercial assay or kitDual-Luciferase Assay SystemPromegaE1910
Commercial assay or kitTransporter 5 Transfection ReagentPolysciences26008–1 A
Chemical compound, drugParathyroid hormone (PTH)GenscriptRP01001
Chemical compound, drugOSMI-1SigmaSML1621-5MG
Chemical compound, drugThiamet-G (TMG)BiosynthMD08856
Chemical compound, drugDoxycycline foodBio-ServS3888
Chemical compound, drugIBMXCAYMAN13347
Chemical compound, drugDexamethasoneSigmaD4902
Chemical compound, drugInsulinSigma91077 C
Chemical compound, drugRosiglitazoneSigmaR2408-10MG
Chemical compound, drugAscorbic acidSigmaA4403-100MG
Chemical compound, drugβ-GlycerophosphateSanta cruzsc-220452
Software, algorithmFlowJBD Life SciencesV10

Animals

All animal experiments were approved by the institutional animal care and use committee of the University of Minnesota (protocol # 2112–39682 A). All the mice were group-housed in light/dark cycle- (6am-8pm light), temperature- (21.5 ± 1.5 °C), and humidity-controlled (30–70%) room, and had free access to water and regular chow (Teklad #2018) unless otherwise indicated. Moist food was provided to constitutive Sp7GFP:Cre animals to circumvent tooth defects and prevent malnutrition. All mice were maintained on a C57BL6 background. Due to the X-chromosome localization of the Ogt gene, only male mice were used in the study if not specified in the text or figures. To suppress Cre activity, designated breeders were fed a diet containing 200 mg/kg doxycycline (Bio-serv, S3888).

BMSC isolation, culture, and differentiation

BMSC were isolated from the long bones as described previously (Zhu et al., 2010). The fragments of long bones were digested with collagenase II for 30 min. The released cells were discarded, and the digested bone fragments were cultivated in the BMSCs growth medium (alpha-MEM supplemented with 10% FBS). Once confluent, cells were switched to either adipogenic differentiation medium (alpha-MEM supplemented with 20% FBS, 500 µM IBMX, 1 µM Dexamethasone, 10 µg/ml Insulin and 1 µM Rosiglitazone) for the first 2 days. The medium was then changed to adipocyte differentiation base medium (α-MEM supplemented with 20% FBS, 10 µg/ml Insulin and 1 µM Rosiglitazone) for the next 4 days followed by oil red O staining. For osteogenic differentiation, cells were induced with osteoblast differentiation medium (α-MEM supplemented with 10% FBS, 0.3 mM ascorbic acid, 10 mM β-glycerophosphate, 0.1 µM Dexamethasone) for 14 days followed by ALP staining or for 28 days followed by Alizarin red staining.

Cell culture, plasmids, and lentiviruses

HEK 293, COS7, and C3H10T1/2 (ATCC, CCL-226) cells were cultured with DMEM plus 10% of FBS. The mouse RUNX2-Myc/DDK plasmid was purchased from OriGene (MR227321), then subcloned into pLV-EF1a-IRES-Hygro (Addgene #85134). Mouse PPARγ2 with a N-terminal MYC tag was subcloned into pLVX-Dsred-puro plasmid. C/EBPβ plasmids were kindly provided by Dr. Xiaoyong Yang at Yale University and then subcloned into pCDH-CMV-P2a-Puro. O-GlcNAc sites were mutated into alanine with Q5 Site-Directed Mutagenesis Kit (NEB#E0554). Lentivirus was packed as previously described (Huang et al., 2022). Briefly, 293 FT cells were transfected with over-expression plasmids pSPAX2, and pMD2.G. Media with lentivirus were filtered and added into C3H10T1/2 cells. Seventy-two hr after infection, cells were then selected with drugs according to the resistance genes they possessed.

Luciferase assay

For Runx2 luciferase assay, empty or RUNX2 vectors were transfected into COS7 cells with Lipofectamine, together with 6xOSE2-luc (Phimphilai et al., 2006) and pGL4-hRluc vectors in which either firefly or Renilla luciferase genes were expressed under the control of the RUNX2-specific or the constitutive SV40 promoter, respectively. After 6 hr, cells were washed three times and with the addition of 50 µM OSMI-1. Cells were incubated for an additional 48 hr in growth medium containing 5% serum. Luminescent signals were generated using the Dual-Luciferase Assay System (Promega). Relative light units (RLU) for the 6xOSE2 reporter were normalized against pGL4-hRLuc values as an internal control for transfection efficiency. For PPARγ2 luciferase assays, C3H10T1/2 cells were transfected with Transporter 5 Transfection Reagent (Polysciences) following manufacture’s protocol. PPARγ2 transcriptional activity was determined using the PPREx3-TK-luc reporter (Addgene, #1015).

Histology

Bone tissues were fixed in formalin solution at 4 °C for 24 hr. Tissue embedding, sectioning, and hematoxylin and eosin staining were performed at the Comparative Pathology Shared Resource of the University of Minnesota. For immunostaining, the tissues were embedded in OCT then cut into 7 µm slides. After three times of PBS wash, the slides were incubated with blocking buffer (3% BSA in PBS) for 1 hr, then immersed with anti-Perilipin (Cell Signaling Technology, #9349) antibody overnight at 4 °C. For immunofluorescence, PBS-washed slides were incubated with a fluorescent secondary antibody at room temperature for 1 hr, and then mounted with VECTASHIELD Antifade Mounting Medium with DAPI after three times of PBS wash. A Nikon system was used for imaging. Goldner’s trichrome and Safranin O staining were performed at Servicebio, China.

micro-CT

The samples were scanned with an in vitro micro-CT device (Skyscan 1272, Bruker micro-CT) with scanning parameters of: Source Voltage = 60 kV, Source Current = 166 µA, exposure 897ms/frame, average of 3 frames per projection, Rotation Step (deg)=0.200 and 0.25 mm Aluminum filter. The specimens were scanned at high resolution (2016×1344 pixels) with an Isotropic voxel size of 7.1 μm. Reconstructions for X-ray projections and re-alignment were performed using the Skyscan software (NRecon and DataViewer) (v. 1.7.3.1, Brüker micro-CT, Kontich, Belgium). Ring artefact and beam hardening corrections were applied in reconstruction. Datasets were loaded into SkyScan CT-Analyzer software for measurement of BMD. Calibration was performed with 0.25- and 0.75 mg/mL hydroxyapatite mice phantoms provided by SkyScan. For cancellous and cortical bone analysis, the scanning regions were confined to the distal metaphysis, 100 slices starting at 0.5 mm proximally from the proximal tip of the primary spongiosa for the cancellous portion and 100 slices starting at 4.5 mm proximally from the center of intercondylar fossa for the cortical portion.

O-GlcNAc mass spectrometry

Myc-tagged PPARγ2 was co-transfected with OGT into 15 cm-dishes of 293T cells and purified by immunoprecipitation with anti-c-Myc agarose beads (Pierce), followed by PAGE gel electrophoresis. The corresponding PPARγ2 band was cut for in gel Trypsin (Promega) digestion. Tryptic peptides were analyzed by on-line LC-MS/MS using an Orbitrap Fusion Lumos (Thermo) coupled with a NanoAcquity UPLC system (Waters) as we previously reported (Liu et al., 2019; Zhao et al., 2022). Peaklists were generated using PAVA (UCSF) and searched using Protein Prospector 5.23.0 against the SwissProt database and a randomized concatenated database with the addition of the recombinant PPARγ2 sequence. HexNAcylated peptides were manually verified.

Real-time RT-PCR

RNA was isolated with Trizol and reverse transcribed into cDNA with the iScript cDNA Synthesis Kit. Real-time RT-PCR was performed using iTaq Universal SYBR Green Supermix and gene-specific primers (Figure 6—source data 1) on a Bio-Rad C1000 Thermal Cycler.

Flow cytometry

For PDGFRa+VCAM1+preadipocytes, BM cells were stained with anti-CD45, anti-Ter119, anti-CD31, anti-PDGFRa, and anti-VCAM1. We gated the PDGFRa+VCAM1+ cells after excluding the CD45+Ter119+CD31+ cells. For B-cell lymphopoiesis, BM cells were stained in PBS containing 1% (w/v) bovine serum albumin on ice for 30 min, with anti-B220 (Life Technologies, 67-0452-82), anti-CD43 (BD Biosciences, 553271), anti-CD24 (Biolegend, 101822), anti-Ly-51 (Biolegend, 108305), anti-CD127 (Tonbo Biosciences, 20–1271 U100), anti-CD25 (Life Technologies, 63-0251-82) and anti-CD19 (Biolegend, 115545). For hematopoietic stem and progenitor cells, BM cells were stained with a cocktail of biotin-conjugated lineage antibodies CD3e, B220, Ter119, Mac-1 and Gr-1 (Biolegend, 133307), CD4 (Biolegend, 100403), CD5 (Biolegend, 100603), CD8 (Biolegend, 100703), followed by Streptavidin-AF488 (Biolegend, 405235). Cells were then stained with CD127-APC (eBioscience, 17-1271-82), c-Kit-APC-eFluor780 (eBioscience, 47-1171-82), Sca-1-Super Bright 436 (eBioscience, 62-5981-82), CD34-PE (Biolegend, 152204) and FcγR-PerCP-eFluor710 (eBioscience, 46-0161-80), CD150-BV605 (Biolegend, 115927), and CD48-BUV395 (BDBioscience, 740236). Fixable Viability Dye was used to exclude dead cells as instructed by the manufacturer. A complete list of used antibodies was shown in Figure 5—source data 1. Flow cytometry was performed on an LSR Fortessa H0081 or X20 and analyzed with FlowJo.

Quantification and statistical analysis

Results are shown as mean ± SEM. N values (biological replicates) and statistical analysis methods are described in figure legends. The statistical comparisons were carried out using two-tailed unpaired Student’s t-test and one-way or two-way ANOVA with indicated post hoc tests with Prism 9 (Graphpad). Differences were considered significant when p<0.05; *, p<0.05; **, p<0.01; ***, p<0.001.

Data availability

All data generated or analyzed during this study are included in the manuscript and supporting file. Supplementary tables have been provided for Mass spectrometry, primer sequences, and antibody list.

References

Article and author information

Author details

  1. Zengdi Zhang

    Department of Integrative Biology and Physiology, University of Minnesota Medical School, Minneapolis, United States
    Contribution
    Formal analysis, Investigation, Methodology, Writing - original draft
    Contributed equally with
    Zan Huang
    Competing interests
    No competing interests declared

  2. Zan Huang

    1. Department of Integrative Biology and Physiology, University of Minnesota Medical School, Minneapolis, United States
    2. Laboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China
    3. National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing, China
    Contribution
    Formal analysis, Funding acquisition, Investigation, Methodology, Writing - original draft
    Contributed equally with
    Zengdi Zhang
    Competing interests
    No competing interests declared

  3. Mohamed Awad

    Department of Medical Anatomical Sciences, College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, Pomona, United States
    Contribution
    Data curation, Investigation, Methodology
    Competing interests
    No competing interests declared

  4. Mohammed Elsalanty

    Department of Medical Anatomical Sciences, College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, Pomona, United States
    Contribution
    Supervision, Methodology
    Competing interests
    No competing interests declared

  5. James Cray

    Department of Biomedical Education and Anatomy, The Ohio State University College of Medicine, and Division of Biosciences, The Ohio State University College of Dentistry, Columbus, United States
    Contribution
    Resources, Methodology
    Competing interests
    No competing interests declared

  6. Lauren E Ball

    Department of Cell and Molecular Pharmacology & Experimental Therapeutics, Medical University of South Carolina, Charleston, United States
    Contribution
    Investigation
    Competing interests
    No competing interests declared

  7. Jason C Maynard

    Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, United States
    Contribution
    Data curation, Investigation, Methodology
    Competing interests
    No competing interests declared

  8. Alma L Burlingame

    Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, United States
    Contribution
    Resources, Supervision
    Competing interests
    No competing interests declared

  9. Hu Zeng

    1. Division of Rheumatology, Department of Internal Medicine, Mayo Clinic, Rochester, United States
    2. Department of Immunology, Mayo Clinic, Rochester, United States
    Contribution
    Supervision, Funding acquisition, Investigation
    Competing interests
    No competing interests declared

  10. Kim C Mansky

    Department of Developmental and Surgical Sciences, School of Dentistry, University of Minnesota, Minneapolis, United States
    Contribution
    Resources, Supervision
    Competing interests
    No competing interests declared

  11. Hai-Bin Ruan

    1. Department of Integrative Biology and Physiology, University of Minnesota Medical School, Minneapolis, United States
    2. Center for Immunology, University of Minnesota Medical School, Minneapolis, United States
    Contribution
    Conceptualization, Resources, Supervision, Funding acquisition, Writing - original draft, Writing - review and editing
    For correspondence
    hruan@umn.edu
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-3858-1272

Funding

National Natural Science Foundation of China (32170847)

  • Zan Huang

National Institutes of Health (R01 AI162678)

  • Hu Zeng

National Institutes of Health (R01 AI139420)

  • Hai-Bin Ruan

National Institutes of Health (R01 AI162791)

  • Hai-Bin Ruan

Dr. Miriam and Sheldon G. Adelson Medical Research Foundation

  • Jason C Maynard
  • Alma L Burlingame

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Acknowledgements

We thank Dr. Xiaoyong Yang for kindly providing wildtype and mutant C/EBPβ plasmids; Dr. Renny Franchesci for providing the 6xOSE2-luc plasmid; and Alexis Nagel for Runx2 luciferase assay. This work was supported by the National Natural Science Foundation of China, China (32170847) to ZH, NIH/NIAID (R01 AI162678) to HZ, the Miriam and Sheldon G Adelson Medical Research Foundation to JCM and ALB, and NIH/NIAID (R01 AI139420 and R01 AI162791) to H-BR.

Ethics

All animal experiments were approved by the institutional animal care and use committee of the University of Minnesota (protocol # 2112-39682A).

Copyright

© 2023, Zhang, Huang et al.

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

Metrics

  • 1,637
    views
  • 299
    downloads
  • 10
    citations

Views, downloads and citations are aggregated across all versions of this paper published by eLife.

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Downloads (link to download the article as PDF)

Open citations (links to open the citations from this article in various online reference manager services)

Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)

  1. Zengdi Zhang
  2. Zan Huang
  3. Mohamed Awad
  4. Mohammed Elsalanty
  5. James Cray
  6. Lauren E Ball
  7. Jason C Maynard
  8. Alma L Burlingame
  9. Hu Zeng
  10. Kim C Mansky
  11. Hai-Bin Ruan
(2023)
O-GlcNAc glycosylation orchestrates fate decision and niche function of bone marrow stromal progenitors
eLife 12:e85464.
https://doi.org/10.7554/eLife.85464

Share this article

https://doi.org/10.7554/eLife.85464

Categories and tags

Research organism

Further reading

    1. Biochemistry and Chemical Biology
    2. Microbiology and Infectious Disease

    Malaria parasites require a divergent heme oxygenase for apicoplast gene expression and biogenesis

    Amanda Mixon Blackwell, Yasaman Jami-Alahmadi ... Paul A Sigala
    Research Article

    Malaria parasites have evolved unusual metabolic adaptations that specialize them for growth within heme-rich human erythrocytes. During blood-stage infection, Plasmodium falciparum parasites internalize and digest abundant host hemoglobin within the digestive vacuole. This massive catabolic process generates copious free heme, most of which is biomineralized into inert hemozoin. Parasites also express a divergent heme oxygenase (HO)-like protein (PfHO) that lacks key active-site residues and has lost canonical HO activity. The cellular role of this unusual protein that underpins its retention by parasites has been unknown. To unravel PfHO function, we first determined a 2.8 Å-resolution X-ray structure that revealed a highly α-helical fold indicative of distant HO homology. Localization studies unveiled PfHO targeting to the apicoplast organelle, where it is imported and undergoes N-terminal processing but retains most of the electropositive transit peptide. We observed that conditional knockdown of PfHO was lethal to parasites, which died from defective apicoplast biogenesis and impaired isoprenoid-precursor synthesis. Complementation and molecular-interaction studies revealed an essential role for the electropositive N-terminus of PfHO, which selectively associates with the apicoplast genome and enzymes involved in nucleic acid metabolism and gene expression. PfHO knockdown resulted in a specific deficiency in levels of apicoplast-encoded RNA but not DNA. These studies reveal an essential function for PfHO in apicoplast maintenance and suggest that Plasmodium repurposed the conserved HO scaffold from its canonical heme-degrading function in the ancestral chloroplast to fulfill a critical adaptive role in organelle gene expression.

    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Endogenous oligomer formation underlies DVL2 condensates and promotes Wnt/β-catenin signaling

    Senem Ntourmas, Martin Sachs ... Dominic B Bernkopf
    Research Article

    Activation of the Wnt/β-catenin pathway crucially depends on the polymerization of dishevelled 2 (DVL2) into biomolecular condensates. However, given the low affinity of known DVL2 self-interaction sites and its low cellular concentration, it is unclear how polymers can form. Here, we detect oligomeric DVL2 complexes at endogenous protein levels in human cell lines, using a biochemical ultracentrifugation assay. We identify a low-complexity region (LCR4) in the C-terminus whose deletion and fusion decreased and increased the complexes, respectively. Notably, LCR4-induced complexes correlated with the formation of microscopically visible multimeric condensates. Adjacent to LCR4, we mapped a conserved domain (CD2) promoting condensates only. Molecularly, LCR4 and CD2 mediated DVL2 self-interaction via aggregating residues and phenylalanine stickers, respectively. Point mutations inactivating these interaction sites impaired Wnt pathway activation by DVL2. Our study discovers DVL2 complexes with functional importance for Wnt/β-catenin signaling. Moreover, we provide evidence that DVL2 condensates form in two steps by pre-oligomerization via high-affinity interaction sites, such as LCR4, and subsequent condensation via low-affinity interaction sites, such as CD2.

    1. Biochemistry and Chemical Biology
    2. Physics of Living Systems

    Reversing protonation of weakly basic drugs greatly enhances intracellular diffusion and decreases lysosomal sequestration

    Debabrata Dey, Shir Marciano ... Gideon Schreiber
    Research Article

    For drugs to be active they have to reach their targets. Within cells this requires crossing the cell membrane, and then free diffusion, distribution, and availability. Here, we explored the in-cell diffusion rates and distribution of a series of small molecular fluorescent drugs, in comparison to proteins, by microscopy and fluorescence recovery after photobleaching (FRAP). While all proteins diffused freely, we found a strong correlation between pKa and the intracellular diffusion and distribution of small molecule drugs. Weakly basic, small-molecule drugs displayed lower fractional recovery after photobleaching and 10- to-20-fold slower diffusion rates in cells than in aqueous solutions. As, more than half of pharmaceutical drugs are weakly basic, they, are protonated in the cell cytoplasm. Protonation, facilitates the formation of membrane impermeable ionic form of the weak base small molecules. This results in ion trapping, further reducing diffusion rates of weakly basic small molecule drugs under macromolecular crowding conditions where other nonspecific interactions become more relevant and dominant. Our imaging studies showed that acidic organelles, particularly the lysosome, captured these molecules. Surprisingly, blocking lysosomal import only slightly increased diffusion rates and fractional recovery. Conversely, blocking protonation by N-acetylated analogues, greatly enhanced their diffusion and fractional recovery after FRAP. Based on these results, N-acetylation of small molecule drugs may improve the intracellular availability and distribution of weakly basic, small molecule drugs within cells.