Pathogen Evolution: Exploring accidental virulence
Not all microbes, such as bacteria and fungi, display virulent properties that allow them to infect host cells and cause disease. While some disease-causing pathogens need a host in order to replicate and survive, some can also live freely in the environment. However, how these pathogens go from innocuously living outside of a host to being able to cause disease within one remains an open question.
One leading theory suggests that these are ‘accidental’ pathogens. According to this ‘accidental virulence theory’, environmental factors cause microbes to gain properties that allow them to survive without a host. While these microbes can exist independently, the adaptations can also make them better equipped to survive, replicate and cause disease if they happen to encounter a potential host (Casadevall and Pirofski, 2007; Figure 1A). For example, microbes that have adapted to living in acidic environments, may also be able to survive in the acidic conditions of animal immune cells, enabling them to infect a host (Hackam et al., 1999). This theory challenges the idea that microbes only acquire the ability to causes disease through exposure and adapting to conditions in a specific host. Now, in eLife, Helen Murphy and colleagues from the College of William and Mary and Vanderbilt University – including Luke Ekdahl and Juliana Salcedo as joint first authors – report evidence supporting the accidental virulence theory using the traditionally non-pathogenic fungus Saccharomyces cerevisiae (Figure 1B; Ekdahl et al., 2023).
Despite S. cerevisiae, which is commonly referred to as baker’s yeast, being considered non-pathogenic, there are some instances in which it can infect humans (Enache-Angoulvant and Hennequin, 2005). This makes it an interesting model for studying how adaptations not related directly to virulence may coincidentally allow the yeast to infect a host. Additionally, there are already many readily available tools for teasing apart the mechanism of evolution and increased pathogenic potential in this model organism.
Ekdahl et al. investigated how S. cerevisiae characteristics were affected by exposure to plastic – a common component of modern environments due to microplastic pollution. The authors grew two separate S. cerevisiae strains on polystyrene beads for 350–400 generations. Throughout this period, only cells that adhered to the beads were transferred to each new bead culture. By doing this, the team created a selection pressure that was specific to the yeast’s interaction with the plastic and not related to how it interacts with the immune system of a potential host. To ensure genetic diversity and mixing of genes amongst the population, fungi that were in their sexual stage were mated during the process.
The experiments showed that selection of plastic-adhering S. cerevisiae led to the evolution of the fungus to display multicellular traits more frequently. Typically, S. cerevisiae is found in its single-celled oval yeast form. Here, the fungus made more elongated branch-like structures called pseudohyphae, better biofilms (durable structures that help microbial communities adhere to surfaces), and multicellular floating structures called ‘flors’.
All three of these multicellular phenotypes have been associated with increased ability of fungi, including S. cerevisiae, to infect mammals by allowing fungal communities to persist and ‘stick’ within the host (Roig et al., 2013). Ekdahl et al. found that some of these multicellular phenotypes, namely the flor and biofilm formation, correlated with plastic adherence. However, pseudohyphae formation was observed even when the fungi displayed low levels of adherence, indicating that it developed independently of this trait and was instead a result of nutrient limitation.
To investigate how these multicellular adaptations impact how well the fungus can cause disease, Ekdahl et al. infected wax moth larvae with S. cerevisiae. As well as allowing relatively rapid screening of a large number of microbes at once, results from this insect model often correspond with infections in mice (Smith and Casadevall, 2021). Interestingly, the findings showed that fungi that had adapted to display multicellularity also showed increased virulence compared to the original strains and adapted isolates that were non-multicellular. This serves as a striking proof of principle that non-living environmental factors, such as plastic surfaces, can coincidentally enhance the pathogenic potential of a microbe.
The work of Ekdahl et al. illustrates several important concepts and has implications for future work on fungal pathogens. First, the findings support the accidental virulence theory by showing that adaptation to an environmental factor, independent of a host, can incidentally increase a microbe’s ability to cause disease. Second, while the genetic basis of the increased multicellularity was not elucidated, the fungal specimens that were generated can be studied in the future to fully understand how the fungi gained these new characteristics. Lastly, showing that plastic adhesion can enhance fungal virulence serves as a warning as environmental pollution with microplastics becomes increasingly ubiquitous (Hale et al., 2020). Consequently, environmental fungi may become better adapted to living on plastic surfaces, resulting in them gaining virulent traits which could potentially contribute to emergence of new types of fungal infection.
References
-
Invasive Saccharomyces infection: a comprehensive reviewClinical Infectious Diseases 41:1559–1568.https://doi.org/10.1086/497832
-
BookPhagosomal acidification mechanisms and functional significanceIn: Gordon S, editors. In Advances in Cellular and Molecular Biology of Membranes and Organelles. Phagocytosis The Host. pp. 299–319.
-
A global perspective on microplasticsJournal of Geophysical Research 125:e2018JC014719.https://doi.org/10.1029/2018JC014719
Article and author information
Author details
Publication history
Copyright
© 2024, Smith
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Metrics
-
- 832
- views
-
- 84
- downloads
-
- 2
- citations
Views, downloads and citations are aggregated across all versions of this paper published by eLife.
Download links
Downloads (link to download the article as PDF)
Open citations (links to open the citations from this article in various online reference manager services)
Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)
Further reading
-
- Evolutionary Biology
Signs of ageing become apparent only late in life, after organismal development is finalized. Ageing, most notably, decreases an individual’s fitness. As such, it is most commonly perceived as a non-adaptive force of evolution and considered a by-product of natural selection. Building upon the evolutionarily conserved age-related Smurf phenotype, we propose a simple mathematical life-history trait model in which an organism is characterized by two core abilities: reproduction and homeostasis. Through the simulation of this model, we observe (1) the convergence of fertility’s end with the onset of senescence, (2) the relative success of ageing populations, as compared to non-ageing populations, and (3) the enhanced evolvability (i.e. the generation of genetic variability) of ageing populations. In addition, we formally demonstrate the mathematical convergence observed in (1). We thus theorize that mechanisms that link the timing of fertility and ageing have been selected and fixed over evolutionary history, which, in turn, explains why ageing populations are more evolvable and therefore more successful. Broadly speaking, our work suggests that ageing is an adaptive force of evolution.
-
- Evolutionary Biology
- Genetics and Genomics
The evolutionary origins of Bilateria remain enigmatic. One of the more enduring proposals highlights similarities between a cnidarian-like planula larva and simple acoel-like flatworms. This idea is based in part on the view of the Xenacoelomorpha as an outgroup to all other bilaterians which are themselves designated the Nephrozoa (protostomes and deuterostomes). Genome data can provide important comparative data and help understand the evolution and biology of enigmatic species better. Here, we assemble and analyze the genome of the simple, marine xenacoelomorph Xenoturbella bocki, a key species for our understanding of early bilaterian evolution. Our highly contiguous genome assembly of X. bocki has a size of ~111 Mbp in 18 chromosome-like scaffolds, with repeat content and intron, exon, and intergenic space comparable to other bilaterian invertebrates. We find X. bocki to have a similar number of genes to other bilaterians and to have retained ancestral metazoan synteny. Key bilaterian signaling pathways are also largely complete and most bilaterian miRNAs are present. Overall, we conclude that X. bocki has a complex genome typical of bilaterians, which does not reflect the apparent simplicity of its body plan that has been so important to proposals that the Xenacoelomorpha are the simple sister group of the rest of the Bilateria.