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    1. Computational and Systems Biology
    2. Microbiology and Infectious Disease

    MiSiC, a general deep learning-based method for the high-throughput cell segmentation of complex bacterial communities

    Swapnesh Panigrahi et al.
    1. Physics of Living Systems
    2. Structural Biology and Molecular Biophysics

    Multi-color fluorescence fluctuation spectroscopy in living cells via spectral detection

    Valentin Dunsing et al.
    1. Chromosomes and Gene Expression
    2. Genetics and Genomics

    A precisely adjustable, variation-suppressed eukaryotic transcriptional controller to enable genetic discovery

    Asli Azizoglu et al.
    A new gene expression tool enables precise control of protein dosage over a large range and paves the way for new genetic discoveries.
    1. Cell Biology
    2. Developmental Biology

    Imaging cytoplasmic lipid droplets in vivo with fluorescent perilipin 2 and perilipin 3 knock-in zebrafish

    Meredith H Wilson et al.
    Fluorescent perilipin zebrafish lines are powerful new tools for studying lipid droplet dynamics in health and disease.
    1. Neuroscience

    Scanned optogenetic control of mammalian somatosensory input to map input-specific behavioral outputs

    Ara Schorscher-Petcu et al.
    A system to generate ‘remote touch’ in freely behaving mice can be utilized to map behavior caused by precise somatosensory inputs.
    1. Microbiology and Infectious Disease
    2. Plant Biology

    Host-associated microbe PCR (hamPCR) enables convenient measurement of both microbial load and community composition

    Derek S Lundberg et al.
    hamPCR is a cost-effective and transformative amplicon sequencing strategy to describe microbiota composition and measure its overall abundance, applicable to all samples in which host and microbial DNA are co-extracted.
    1. Cancer Biology
    2. Genetics and Genomics

    Multi-syndrome, multi-gene risk modeling for individuals with a family history of cancer with the novel R package PanelPRO

    Gavin Lee et al.
    1. Developmental Biology

    Image3C, a multimodal image-based and label-independent integrative method for single-cell analysis

    Alice Accorsi et al.
    Image3C is a new image-based analysis of cell population composition for research organisms in which detailed cellular phenotypes are unknown or for which species-specific reagents are not available.
    1. Neuroscience
    2. Physics of Living Systems

    Fast deep neural correspondence for tracking and identifying neurons in C. elegans using semi-synthetic training

    Xinwei Yu et al.
    A deep neural network trained on semi-synthetic data learns to quickly track and identify neurons in Caenorhabditis elegans.
    1. Neuroscience
    2. Physics of Living Systems

    Rapid mechanical stimulation of inner-ear hair cells by photonic pressure

    Sanjeewa Abeytunge et al.
    A new method is described to apply rapid and uniform mechanical forces to vestibular and cochlear hair cells.
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