New intracellularly-expressed llama anti-EPS15 nanobodies reveal that pioneer clathrin-coat components function upstream of AP-2 clathrin adaptor deposition at the plasma membrane.
An unbiased RNAseq based strategy to identify targetable pathways and a streamlined lentiviral system provide a state of the art advance on direct fibroblast to neuron conversion.
Maria Pernemalm, AnnSofi Sandberg ... Janne Lehtiö
Comprehensive mass spectrometry analysis of human plasma proteome reveals tissue leakage proteins, describes variability between individual plasma proteomes and demonstrates protein transfer across the placenta during pregnancy.
The design and validation of an oligo-based DNA FISH library that targets the entire Caenorhabditis elegans genome at chromosome, three megabase, and 500 kb scales.
By using a specialized camera that corrects for eye blur, millions of single-blood-cells are imaged, and their speed measured, as they travel through the largest-to-smallest vessels of the retina.
Loïc Binan, François Bélanger ... Santiago Costantino
Single cells from a large heterogeneous population can be identified, isolated and clonally expanded using commonly available microscopy equipment and simple reagents, based solely on visual characteristics.
A transcriptome dataset of nearly 200 genetically identified mouse neuronal cell types revealed that short low-noise homeobox transcription factors and long neuronal effector genes best distinguish neuronal cell types.
O Sascha Yousefi, Matthias Günther ... Wolfgang WA Schamel
Controlling the duration of ligand binding to the T cell receptor by light shows that T cells discriminate stimulatory from non-stimulatory ligands by measuring the dynamics of ligand binding.
The F-box and U-box E3 ubiquitin ligase decoy library is a powerful tool for the functional characterization of redundant E3 ubiquitin ligases, like MAC3A and MAC3B, novel circadian clock regulators.
