Figure 3. | Examining kinesin processivity within a general gating framework

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Examining kinesin processivity within a general gating framework

Figure 3.

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Stanford University, United States; Pennsylvania State University, United States
Figure 3.
Download figureOpen in new tabFigure 3. Extending the NL by a single AA compromises processivity.

Mean run lengths as a function of applied force (mean ± SE; N = 49–818) for the constructs studied, acquired with an optical force clamp in the presence of 2 mM ATP (solid circles; color-coded according to the legend). DmK-WT (Milic et al., 2014) and hindering load data sets for DmK-3AA (Andreasson et al., 2015) are reproduced from our previous work. For all constructs, mean run lengths exhibited significant asymmetry, depending upon the direction of load. To obtain the unloaded run length (L0) and characteristic distance parameter (δL) for each construct, run length (L) data for hindering (−6 to 0 pN) and assisting loads (+2 to +20 pN) were separately fit to exponentials (solid lines; color-coded according to the legend) of the form L(F)=L0exp[|F|δL/kBT], where F is the force applied by the optical trap and kBT is Boltzmann's constant times the absolute temperature; parameter values are in Table 1. Inset cartoon, a graphical representation of the experimental geometry of the single-molecule assay (not to scale).