Figure 5—figure supplement 1. | Lipid-mediated regulation of SKN-1/Nrf in response to germ cell absence

Open accessCopyright infoDownload PDFDownload figuresRelated content

Lipid-mediated regulation of SKN-1/Nrf in response to germ cell absence

Figure 5—figure supplement 1.

Affiliation details

Joslin Diabetes Center, United States; Harvard Medical School, United States; Boston University, United States
Figure 5—figure supplement 1.
Download figureOpen in new tabFigure 5—figure supplement 1. Representative ORO staining images with quantification.

Representative images divided into approximate quintiles, ordered by mean pixel intensity, are shown for each strain. BF images are presented on the left and background subtracted images with quantification on the right. (A) glp-1(bn18ts) and skn-1(zu135) mutants. (B) glp-1(ts) mutants treated with skn-1 and sbp-1 RNAi. Increased staining was observed both with skn-1 mutants and skn-1 RNAi. RNAi against sbp-1, which is required for lipogenesis (Yang et al., 2006), decreases ORO staining in both WT and glp-1(ts) genetic backgrounds. (C) glp-1(e2141ts) and daf-16(mu86) mutants. Numbers indicate mean pixel intensity above background (see ‘Materials and methods’ for additional details).

DOI: http://dx.doi.org/10.7554/eLife.07836.012