Figure 1—figure supplement 1. | Epigenomic landscapes of retinal rods and cones

Open accessCopyright infoDownload PDFDownload figures

Epigenomic landscapes of retinal rods and cones

Figure 1—figure supplement 1.

Affiliation details

Johns Hopkins University School of Medicine, United States; The Salk Institute for Biological Studies, United States; Howard Hughes Medical Institute, The Salk Institute for Biological Studies, United States; Janelia Research Campus, Howard Hughes Medical Institute, United States; University of California San Diego, United States; The University of Western Australia, Australia; Johns Hopkins University, United States
Figure 1—figure supplement 1.
Download figureOpen in new tabFigure 1—figure supplement 1. Genetic labeling of mouse rod and cone photoreceptor nuclei.

(A–B) Immunohistochemistry for GFP (green) showing labeling of rod photoreceptors in adult Lmopc1-Cre; R26-CAG-LSL-Sun1-sfGFP-myc retina. Lmopc1-Cre is a rod-specific Cre transgene controlled by the mouse opsin promoter (Le et al., 2006). GFP is restricted predominantly to rods in the outer nuclear layer (ONL). Scale bar (B) 20 µm. (C) Immunohistochemistry for GFAP (red) in adult Lmopc1-Cre; R26-CAG-LSL-Sun1-sfGFP-myc retina. GFAP is normally expressed by retinal astrocytes and is upregulated in reactive Müller glia during retinal stress. Although astrocytes in this retina are strongly GFAP+, there is no detectable GFAP labeling in Müller glia. OS, outer segments; INL, inner nuclear layer; GCL, ganglion cell layer. Native GFP fluorescence is shown (green). Scale bar: 50 µm. (DE) Immunohistochemistry for GFP (green) and labeling with peanut agglutinin (PNA, red), a marker for cones, in adult HRGP-Cre; R26-CAG-LSL-Sun1-sfGFP-myc mice. HRGP-Cre is a cone-specific Cre transgene controlled by the human red/green pigment promoter (Le et al., 2004). HRGP-Cre predominantly recombines cones, as seen by their location, labeling with PNA, and DAPI staining (Solovei et al., 2009). Scale bars: 50 µm (D) and 10 µm (E). (F) A representative flow cytometry profile of nuclei sorted from HRGP-Cre; R26-CAG-LSL-Sun1-sfGFP-myc retinas. The thresholds used to define singlet nuclei (left) and GFP+ nuclei (right) are outlined in black.

DOI: http://dx.doi.org/10.7554/eLife.11613.004