Figure 2—figure supplement 3. | Human kinetochores are swivel joints that mediate microtubule attachments

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Human kinetochores are swivel joints that mediate microtubule attachments

Figure 2—figure supplement 3.

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University of Warwick, United Kingdom
Figure 2—figure supplement 3.
Download figureOpen in new tabFigure 2—figure supplement 3. Kinetochore swivel is also present between other pairs of kinetochore markers.

Example images of other pairs of kinetochore proteins tagged by fluorescent markers with visible swivel, including (clockwise from top left): CENP-A-Alexa488 and Ndc80-tagRFP; eGFP-CENP-A and Nnf1-Alexa594; Bub3-eGFP and mCherry-CENP-A; GFP-CENP-O and Ndc80-tagRFP; GFP-CENP-C and Ndc80-tagRFP; GFP-CENP-C and mCherry-Ndc80; CENP-C-Alexa488 and mCherry-Ndc80; and eGFP-CENP-A and mCherry-Mis12. Green dashed lines are the sister-sister axes, white dashed lines are intra-kinetochore axes. Scale bars 500 nm.

DOI: http://dx.doi.org/10.7554/eLife.16159.012