Figure 3. | Toxoplasma gondii F-actin forms an extensive filamentous network required for material exchange and parasite maturation

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Toxoplasma gondii F-actin forms an extensive filamentous network required for material exchange and parasite maturation

Figure 3.

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University of Glasgow, United Kingdom; Cancer Research United Kingdom Beatson Institute, United Kingdom; University of Vermont, United States
Figure 3.
Download figureOpen in new tabFigure 3. Filamentous actin can be visualized by expression of Cb-Halo and Cb-Emerald.

(A) Sections of parasite vacuoles in two, four, and eight-cell stage stably transfected with Cb-Halo or Cb-Emerald (red). A filamentous network connecting individual cells (arrows) can be visualised using Cb-Halo and Cb-Emerald. Parasites were co-stained with IMC marker, GAP45 (green), DAPI staining (blue) Scale bar 5 µm. (B) Images of larger parasite vacuoles containing 16 or 32 parasites transfected with Cb-Emerald (red). Parasites were co-stained with IMC marker GAP45 (green). Note the formation of the extensive intravacuolar network with long filamentous tubes (see also Figure 7). Scale bar 10 µm (C) Top. Image of parasites expressing SAG1ΔGPI-GFP to label the dense granules and low and high levels of Cb-Halo. Scale bar 10 µm. Bottom. Directed granule run frequency in control (non-expressing) parasites and parasites expressing Cb-Halo at high and low levels. ***p<0.0001; students t-test. Total number of directed runs counted in control, low expression and high expression samples were 183, 150 and 1 respectively. Total number of vacuoles analysed from control, low expression and high expression were 19,17 and 14, respectively, from two independent transfections. Error bars are S.E.M.

DOI: http://dx.doi.org/10.7554/eLife.24119.009