Figure 8. | Toxoplasma gondii F-actin forms an extensive filamentous network required for material exchange and parasite maturation

Open accessCopyright infoDownload PDF

Toxoplasma gondii F-actin forms an extensive filamentous network required for material exchange and parasite maturation

Figure 8.

Affiliation details

University of Glasgow, United Kingdom; Cancer Research United Kingdom Beatson Institute, United Kingdom; University of Vermont, United States
Figure 8.
Download figureOpen in new tabFigure 8. Parasite-derived extracellular vesicles are transported in an F-actin dependent manner.

(A) Parasites co-expressing GAPM3-YFP and Cb-Halo were imaged every 6 min for 5 hr, F-actin can be seen initially connecting the basal end of the parasites before accumulating beneath the forming daughter cells where it appears to concentrate towards the rear of the new daughters during emergence and recycling of the maternal IMC. Note the sudden collapse of the mother IMC into vesicles that appear to move towards the IMC of the nascent daughter cells (arrow). Scale bar 10 μm. (B) In parasites endogenously expressing GAPM1a-YFP, extracellular vesicles could be observed in close apposition to Cb-Halo labelled filaments (arrow). Parasites expressing Cb-Halo were imaged every second for up to 5 min. Extracellular vesicles positive for GAPM1a-YFP were observed to move along F-actin filaments (arrows). Scale bar, 5 μm. (C) The number of vesicles per vacuole that moved within 5 min of imaging were quantified in the presence and absence of 500 nM Cyt-D. At least 60 vacuoles were counted over three independent experiments. ****p<0.0001.

DOI: http://dx.doi.org/10.7554/eLife.24119.020