Jessica E Scholey, Stanley Nithianantham ... Jawdat Al-Bassam
The Bipolar Assembly domain of kinesin-5 comprises an anti-parallel four-helix bundle, which explains how kinesin-5 subunits assemble into bipolar tetramers with two motile ends that transmit forces while crosslinking and sliding adjacent microtubules during mitosis.
Tatyana Bodrug, Elizabeth M Wilson-Kubalek ... Jawdat Al-Bassam
Biochemical, single molecule, cell and structural biology studies reveal an interaction between the kinesin-5 tail and motor domains regulating high-force production, which is critical for microtubule sliding motility.
Precise hydrogen-bond stabilization of the neck linker docking onto the catalytic motor domain, is crucial for motor activity and intracellular functions of the bi-directional mitotic kinesin-5 Cin8.
Andrea Serra-Marques, Maud Martin ... Anna Akhmanova
The function and distribution of kinesin motors on exocytotic vesicles is dissected and visualized through a combination of gene knockout experiments, high-resolution microscopy and advanced data analysis.
Gabriel Cavin-Meza, Michelle M Kwan, Sarah M Wignall
Rapid depletion of dynein from oocytes yielded new insights into acentrosomal pole organization and also led to the first evidence that the kinesin-5 motor BMK-1 plays a role in microtubule organization during C. elegans meiosis.
Christopher Edelmaier, Adam R Lamson ... Meredith D Betterton
A computational model of fission yeast mitosis can interrogate mechanisms required for successful mitosis, the origin of spindle length fluctuations, and spindle force balance during assembly.
Charlotte Guyomar, Clément Bousquet ... Denis Chrétien
Tubulin engages unique lateral interactions without longitudinal ones during microtubule polymerization, leaving holes of a few subunits size potentially at the origin of tubulin exchange within their shaft.
Jonas R Knudsen, Kaspar W Persson ... Thomas Elbenhardt Jensen
Microtubule-dependent movement of the glucose transporter GLUT4 is important for insulin-stimulated muscle glucose uptake, involves the motor protein KIF5B, and is impaired in muscle from diet-induced obese mice.