Thomas E Sladewski, Neil Billington ... Kathleen M Trybus
mRNA cargo orchestrates the activation of an in vitro reconstituted dynein-dynactin-Bicaudal D-Egalitarian complex for robust processive motion on microtubules.
Thomas Pohlmann, Sebastian Baumann ... Michael Feldbrügge
An endosomal component employs a novel PAM2-like motif to recruit a key RNA-binding protein, which explains how mRNAs and associated ribosomes are attached to endosomes during coupled transport.
Mark A McClintock, Carly I Dix ... Simon L Bullock
Reconstitution of dynein-based mRNA transport with purified components reveals a pivotal role of an RNA localisation signal in controlling the assembly and activity of the translocation machinery.
Ross Nieuwburg, Dmitry Nashchekin ... Daniel St Johnston
Dynactin acts as an anti-catastrophe factor that extends microtubule growth; posteriorly elucidating a new essential step in oskar mRNA localisation and providing a novel mechanism by which motor-dependent transport can amplify the polarity of MT networks.
Philipp Khuc Trong, Hélène Doerflinger ... Raymond E Goldstein
A three-dimensional description of the cytoskeletal arrangement, cytoplasmic flows, and cargo transport in stage 9 Drosophila oocytes accurately reproduces mRNA localizations in wild-type and mutant oocytes.
Posterior determination of Drosophila oocyte is defined by competition between kinesin-1 removing posterior determinants from the cortex and myosin-V anchoring them.
In vitro reconstitution of mRNA motility reveals the basis of directionally biased motion by groups of motors, their response to potential obstacles, and the consequences of reaching microtubule ends.
Helena Jambor, Vineeth Surendranath ... Pavel Tomancak
Genome wide analysis reveals that many transcripts are localized in cells of the ovary and that the localization status of mRNAs changes over time and in different cell types.