7,216 results found
    1. Neuroscience

    Hipposeq: a comprehensive RNA-seq database of gene expression in hippocampal principal neurons

    Mark S Cembrowski, Lihua Wang ... Nelson Spruston
    Gene expression was analyzed in hippocampal principal cells, enabling cellular phenotyping and revealing novel organizational principles; complementing this, a publicly available website is released to provide outside analysis and visualization of hippocampal RNA-seq data in an accessible fashion.
    1. Genetics and Genomics
    2. Neuroscience

    Probe-Seq enables transcriptional profiling of specific cell types from heterogeneous tissue by RNA-based isolation

    Ryoji Amamoto, Mauricio D Garcia ... Constance L Cepko
    Probe-Seq is a cell type specific bulk RNA sequencing method that can be applied to a wide-ranging tissue types from both vertebrates and invertebrates without genetic labeling.
    1. Computational and Systems Biology
    2. Genetics and Genomics

    Identifying gene expression programs of cell-type identity and cellular activity with single-cell RNA-Seq

    Dylan Kotliar, Adrian Veres ... Pardis C Sabeti
    A novel algorithm, consensus non-negative matrix factorization (cNMF), accurately identifies gene expression programs underlying cell-type identity and cellular activities from single-cell RNA-Seq data.
    1. Chromosomes and Gene Expression
    2. Microbiology and Infectious Disease

    A new protocol for single-cell RNA-seq reveals stochastic gene expression during lag phase in budding yeast

    Abbas Jariani, Lieselotte Vermeersch ... Kevin J Verstrepen
    A new high-throughput method for single-cell RNA-seq in yeast cells shows how stochastic expression of glucose-repressed genes contributes to cell-to-cell differences during adaptation to an environmental change.
    1. Chromosomes and Gene Expression

    Chromatin-associated RNA sequencing (ChAR-seq) maps genome-wide RNA-to-DNA contacts

    Jason C Bell, David Jukam ... Aaron F Straight
    ChAR-seq is a massively parallelized de novo RNA mapping assay, which is capable of generating hundreds to thousands of RNA-binding maps with no a priori knowledge of target RNAs.
    1. Developmental Biology

    Single nuclei RNA-seq of mouse placental labyrinth development

    Bryan Marsh, Robert Blelloch
    A time-course of single nuclei RNA-seq of the mouse placenta identifies trophoblast subtypes and the genes, signaling events, and transcriptional networks important for their differentiation, maintenance, and function.
    1. Developmental Biology

    The Drosophila Nab2 RNA binding protein inhibits m6A methylation and male-specific splicing of Sex lethal transcript in female neuronal tissue

    Binta Jalloh, Carly L Lancaster ... Ken Moberg
    The disease-associated RNA binding protein Nab2 is required to inhibit m6A RNA methylation in female Drosophila neuronal tissue and controls key splicing events such as sex-specific splicing of the Sex-lethal RNA.
    1. Chromosomes and Gene Expression
    2. Developmental Biology

    Emergent dynamics of adult stem cell lineages from single nucleus and single cell RNA-Seq of Drosophila testes

    Amelie A Raz, Gabriela S Vida ... Margaret T Fuller
    Single nucleus and single cell RNA-seq data uncover novel transcriptomic perspectives in Drosophila testis biology and provide access to user-friendly tools for further scientific discovery in the field.
    1. Developmental Biology
    2. Genetics and Genomics

    Novel cell types and developmental lineages revealed by single-cell RNA-seq analysis of the mouse crista ampullaris

    Brent A Wilkerson, Heather L Zebroski ... Olivia Bermingham-McDonogh
    The elucidation of the cellular composition of the crista and the genes expressed in each cell type is a critical step toward understanding inner ear development, function, and vestibulopathies.
    1. Developmental Biology

    An improved zebrafish transcriptome annotation for sensitive and comprehensive detection of cell type-specific genes

    Nathan D Lawson, Rui Li ... Lihua Zhu
    Computational efforts yield a zebrafish transcriptome annotation that provides more sensitive and comprehensive gene detection over existing annotations when used for bulk or single-cell RNA-seq.

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