A novel family of nucleolus-enriched ncRNAs forms allele-specific territories on the nucleolar organizing region (NOR)-containing acrocentric chromosomes.

A score-based read selection strategy enables the assembly of novel full-length ribosomal RNA sequences for mosquitoes, which improves the physical and computational removal of interfering ribosomal RNA reads in RNA-seq and provides another molecular marker for taxonomic and phylogenetic inquiries.

The Dengue virus contains discretely folded RNA structures that confer the ability to resist a powerful host cell enzyme and produce a disease-inducing noncoding RNA.

E. coli ribosomes incapable of base-pairing with the Shine-Dalgarno sequence are still selective for annotated start sites, indicating these sites are hard-wired for initiation by other mRNA features.

An ancestral helical bundle governs whether the crRNA-processing nuclease of CRISPR-Cas12a is exploited by a phage-encoded allosteric anti-CRISPR.

The structures of full-length Las1-Grc3 complexes provide crucial insights into the activation mechanism of HEPN nuclease and the pathway of pre-rRNA processing in ribosome biosynthesis.

Directed evolution of the resistance enzyme Cfr under antibiotic selection identifies increased Cfr expression and stability as strategies to boost resistance and reveals that Cfr modification of the ribosome confers resistance by sterically occluding binding of antibiotics.

A mathematical model of bias in marker-gene and metagenomic sequencing measurements explains systematic errors in defined mixtures of microbial species, and enables quantitative and reproducible investigation of biological communities.

Genetic variation influencing the gut microbiome is abundant among incipient mammalian host species, and cospeciating bacterial taxa display a unique genetic architecture.

Integrative structural biology reveals a conformational equilibrium in the Box C/D methylation enzyme that regulates the extent of site-specific 2'-O-rRNA methylation in dependence of the RNA sequence.