The core autophagy protein Atg8 plays non-autophagy roles through binding to an integral membrane protein Hfl1, which harbors an unusual Atg8-interacting motif.
Masroor Ahmad Paddar, Fulong Wang ... Vojo Deretic
Membrane atg8ylation but not canonical autophagy control retromer-dependent endosomal sorting of cargo, including glucose transporter GLUT1, of general import and of significance for control of tuberculosis.
Autophagic cargo receptors recruit the E3-like enzyme for Atg8 lipid conjugation to the cargo and thereby promote local formation of Atg8-positive autophagosomal membranes.
Phosphorylation of a core trafficking component, the COPII coat subunit Sec24, regulates cross-talk between the secretory and autophagy machinery during starvation.
Jessica T Chang, Caroline Kumsta ... Malene Hansen
Autophagic flux assays in the nematode Caenorhabditis elegans suggest that autophagy decreases during normal aging, whereas long-lived daf-2 and glp-1 mutants maintain autophagic capacity in distinct spatiotemporal-specific manners to extend lifespan.
Kumi Harada, Tetsuya Kotani ... Hitoshi Nakatogawa
How the autophagy-related E3 complex localizes to pre-autophagosomal structures and a non-E3 function of the complex were revealed, significantly advancing our understanding of the nucleation step in autophagosome formation.
A mechanistic dissection of how autophagy kinase Atg1 is activated in fission yeast reveals surprising differences from the known Atg1 activation mechanisms previously defined in budding yeast.
