Prateek D Bansal, Maia Kinnebrew ... Diwakar Shukla
Multiple competing hypotheses concerning the cholesterol accessibility of oncoprotein Smoothened are quantitatively assessed and experimentally validated, showing that cholesterol takes two pathways from either membrane leaflet to the binding sites.
Mahesh A Vibhute, Corbin Machatzke ... Hannes Mutschler
Structure-guided engineering of a bacterial retron enables intracellular production of a light-up DNA aptamer whose purified DNA retains dye-activated fluorescence, establishing retrons as a scalable route to cellular aptamer synthesis.
Gabriel Valentin Senn, Leon Nissen, Yaakov Benenson
Design and integration of various biomolecular sensors for over-activated rat sarcoma (RAS) created synthetic gene circuits that distinguish mutant versus wild-type signaling and express a therapeutic protein to kill RAS-driven cancer cells.
