By identifying cohesin's DNA entry gate(s) and through understanding the corresponding topology between the two the mechanism behind cohesin's activity is slowly deciphered and will aid in understanding how the SMC family at large functions.
X-ray crystal structures capture ATP-dependent chalcogenide exchange from selenocyanate at the nitrogenase Fe protein cluster in the absence of the MoFe protein, an unexpected result as the Fe protein cluster is not traditionally perceived as a site of substrate binding.
The flavoprotein NrdI in class Ib ribonucleotide reductase controls superoxide generation and metal site oxidation by tuning the redox properties of its flavin cofactor via steric strain induced by the formation of the R2b–NrdI protein complex.
Conall Mc Guinness, James C Walsh ... Till Böcking
Arc-shaped PFO oligomers consisting of at least four subunits can insert into the membrane in a kinetically controlled process opening a transmembrane pore, whereby post-insertion growth may ultimately lead to formation of a complete ring.
Tracking proteins in live cells is challenging due to technical limitations and biological complexity, but approaches based in Bayesian nonparametrics stand a decent chance at recovering a target protein's dynamic profile from noisy data.
