Browse our latest Biochemistry and Chemical Biology articles

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    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Membrane-mediated dimerization potentiates PIP5K lipid kinase activity

    Scott D Hansen, Albert A Lee ... Jay T Groves
    Dimerization promotes nonlinear kinetics in PIP5K-dependent lipid phosphorylation reactions.
    1. Biochemistry and Chemical Biology

    DNA passes through cohesin’s hinge as well as its Smc3–kleisin interface

    James E Collier, Kim A Nasmyth
    By identifying cohesin's DNA entry gate(s) and through understanding the corresponding topology between the two the mechanism behind cohesin's activity is slowly deciphered and will aid in understanding how the SMC family at large functions.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Selenocyanate derived Se-incorporation into the nitrogenase Fe protein cluster

    Trixia M Buscagan, Jens T Kaiser, Douglas C Rees
    X-ray crystal structures capture ATP-dependent chalcogenide exchange from selenocyanate at the nitrogenase Fe protein cluster in the absence of the MoFe protein, an unexpected result as the Fe protein cluster is not traditionally perceived as a site of substrate binding.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Redox-controlled reorganization and flavin strain within the ribonucleotide reductase R2b–NrdI complex monitored by serial femtosecond crystallography

    Juliane John, Oskar Aurelius ... Martin Högbom
    The flavoprotein NrdI in class Ib ribonucleotide reductase controls superoxide generation and metal site oxidation by tuning the redox properties of its flavin cofactor via steric strain induced by the formation of the R2b–NrdI protein complex.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Single-molecule analysis of the entire perfringolysin O pore formation pathway

    Conall Mc Guinness, James C Walsh ... Till Böcking
    Arc-shaped PFO oligomers consisting of at least four subunits can insert into the membrane in a kinetically controlled process opening a transmembrane pore, whereby post-insertion growth may ultimately lead to formation of a complete ring.
    1. Biochemistry and Chemical Biology
    2. Physics of Living Systems

    Recovering mixtures of fast-diffusing states from short single-particle trajectories

    Alec Heckert, Liza Dahal ... Xavier Darzacq
    Tracking proteins in live cells is challenging due to technical limitations and biological complexity, but approaches based in Bayesian nonparametrics stand a decent chance at recovering a target protein's dynamic profile from noisy data.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Multiple ciliary localization signals control INPP5E ciliary targeting

    Dario Cilleros-Rodriguez, Raquel Martin-Morales ... Francesc R Garcia-Gonzalo
    Four conserved ciliary localization signals in the Joubert syndrome-associated INPP5E phosphoinositide phosphatase control its ciliary accumulation.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Multi-step recognition of potential 5' splice sites by the Saccharomyces cerevisiae U1 snRNP

    Sarah R Hansen, David S White ... Aaron A Hoskins
    The yeast U1 snRNP recognizes multiple features of target RNAs to reversibly identify splicing-competent 5' splice sites.