Browse our latest Biochemistry and Chemical Biology articles

Page 4 of 119
    1. Biochemistry and Chemical Biology
    2. Microbiology and Infectious Disease

    An essential, kinetoplastid-specific GDP-Fuc: β-D-Gal α-1,2-fucosyltransferase is located in the mitochondrion of Trypanosoma brucei

    Giulia Bandini et al.
    The activity, localization, and essentiality of TbFUT1, together with its ability to complement Leishmania parasites lacking the homologous gene, suggest the presence of an uncommon and conserved mitochondrial fucosylation pathway required for kinetoplastid parasites viability.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Bipartite binding and partial inhibition links DEPTOR and mTOR in a mutually antagonistic embrace

    Maren Heimhalt et al.
    The inhibitory protein DEPTOR make two widely separated interactions with mTOR that are both necessary for its unique partial mTOR inhibition, and DEPTOR is a more potent inhibitor of mutation- or RHEB-activated mTORC1 than basal mTORC1.
    1. Biochemistry and Chemical Biology
    2. Computational and Systems Biology

    Addressing shortfalls of laboratory HbA1c using a model that incorporates red cell lifespan

    Yongjin Xu et al.
    The person-specific adjusted HbA1c addresses non-glycaemic variation in laboratory HbA1c due to red blood cell lifespan differences, potentially providing a better marker to predict diabetes complications and guide glycaemic management.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Edge-strand of BepA interacts with immature LptD on the β-barrel assembly machine to direct it to on- and off-pathways

    Ryoji Miyazaki et al.
    In vivo crosslinking approaches revealed that the edge-strand of Escherichia coli metallopeptidase BepA interacts with an assembly intermediate of β-barrel outer membrane protein LptD on the outer membrane protein translocon for its targeting to degradation or maturation.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    An engineered transcriptional reporter of protein localization identifies regulators of mitochondrial and ER membrane protein trafficking in high-throughput CRISPRi screens

    Robert Coukos et al.
    HiLITR is a molecular reporter of protein localization that enables straightforward, large-scale, fluorescence-activated cell sorting-based discovery of genes that regulate complex cellular processes, such as mitochondrial and ER tail-anchored protein trafficking.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    β-hydroxybutyrate accumulates in the rat heart during low-flow ischaemia with implications for functional recovery

    Ross T Lindsay et al.
    β-Hydroxybutyrate accumulates in the perfused rat heart during ischaemia, driven by flux through both HMGCS and SCOT, with implications for functional recovery.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Characterization of the ABC methionine transporter from Neisseria meningitidis reveals that lipidated MetQ is required for interaction

    Naima G Sharaf et al.
    NmMetQ is a lipoprotein that tethers to membranes via a lipid anchor and has dual function and localization, playing a role in NmMetNI-mediated transport at the inner membrane and moonlighting on the bacterial surface.
    1. Biochemistry and Chemical Biology

    Concerted conformational dynamics and water movements in the ghrelin G protein-coupled receptor

    Maxime Louet et al.
    A combination of state-of-the-art biochemical, biophysical, and computational methods revealed that water molecules play a central role in signal propagation through G protein-coupled receptors.
    1. Biochemistry and Chemical Biology

    Disintegration promotes protospacer integration by the Cas1-Cas2 complex

    Chien-Hui Ma et al.
    Disintegration, regarded as an abortive side reaction antithetical to DNA transposition, can promote protospacer integration at the CRISPR locus via DNA repair pathways.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    McsB forms a gated kinase chamber to mark aberrant bacterial proteins for degradation

    Bence Hajdusits et al.
    The McsB kinase is critical for protein quality control in Gram-positive bacteria, assembling a gated phosphorylation chamber during heat-shock conditions to selectively mark misfolded proteins for degradation.