Empirical substitution matrices from experimental measurements on 31,614 variants accurately predict cellular protein abundance changes using only amino-acid substitution and whether the site is buried or exposed.
The optimized protocol for isolating small extracellular vesicles from small blood volumes, confirmed by independent methods including cryo-electron microscopy, ensures superior purity and high yield for reliable biomarker detection.
The dynamic internal fluidity of nuclear MORC2 condensates, rather than their mere assembly, is strictly required for transcriptional regulation and is selectively disrupted by neuropathy-linked mutations.
An evolution-guided framework is proposed to accelerate the discovery and therapeutic targeting of understudied dark channels by integrating sequence, structure, and functional data from diverse organisms.
Biochemical and genetic evidence identifies ATP5I as a biguanide target, redefining biguanide action through ATP synthase regulation and assembly, mitochondrial architecture, and mitochondrial protein turnover.
