Browse our latest Biochemistry and Chemical Biology articles

Single particle cryo-electron microscopy associated with functional studies reveal a critical role of N- and C-terminal tails in the autoinhibition of the disease-related ATP8B1-CDC50A lipid flippase, the possible role of phosphorylation in autoinhibition relief, and strong activation by PI(3,4,5)P₃.

Poly(ADP-ribose) (PAR) Trackers (PAR-Ts) are optimized PAR sensors that allow the detection and quantification of PAR levels in extracts, living cells, and living tissues with high sensitivity, and with temporal and spatial precision, in many different experimental and biological systems.

A novel mechanism of intramembrane protease regulation involves a protein blocking the active site of the enzyme.

Spermidine-mediated superoxide generation affects iron homeostasis in Escherichia coli.

The proteasomal deubiquitinase UCHL5/UCH37 uses a face distinct from the canonical ubiquitin binding site to engage K48-linked ubiquitin chains and catalyze chain debranching.

Development, validation, and use of an effector membrane translocation biosensor platform reveals G protein coupling selectivity signatures for 100 GPCRs that range from remarkable selectivity to full promiscuity toward the different G protein subtypes.

Multiple cryo-electron microscopy structures of a plant ABC transporter exporting glutathione derivatives captured a closed conformation proposed to enforce the export directionality, while a complex central to the coupling mechanism containing both glutathione and closed nucleotide binding domains remains elusive.

Co-aggregation, amyloid formation, and unidirectional cross-seeding of prolactin and galanin for their efficient storage in secretory granules of pituitary and subsequent functional hormone release.

The crenarchaeon Sulfolobus synthesizes the N-glycan core in the identical way as all Eukaryotes, which strengthens the hypothesis that the eukaryotic N-glycosylation is acquired from an ancient archaeon during eukaryogenesis.

Identification of a heparan sulfate proteoglycan with a dedicated function in unconventional protein secretion reveals an intimate relationship between fibroblast growth factor 2 and Glypican-1 in cancer biology.