Interactions of eIF4A and eIF4E with specific amino-acids in the N-terminal domain of DEAD-box helicase Ded1 enhance bulk polysome assembly and translation of reporter mRNAs with structured leaders in vivo.
Reconstitution of DNA loop extrusion in cellular contexts using Xenopus egg extracts shows that condensin extrudes DNA loops non-symmetrically in metaphase, whereas cohesin extrudes DNA loops symmetrically in interphase.
Alisha Schlichter, Margaret M Kasten ... Bradley R Cairns
A SWI/SNF-family chromatin remodeling subcomplex from yeast is identified (RSC1) that is specialized to slide nucleosomes residing on DNA sequences that confer partial nucleosome unwrapping, with assistance from accessory factors.
Edward J Banigan, Aafke A van den Berg ... Leonid A Mirny
Seemingly contradictory findings of single-molecule and in vivo experiments on a major mechanism of chromosome organization are reconciled by computationally investigating mechanisms of loop extrusion that are consistent with both.
Unbiased ChIP-seq screens and genetic knockouts of large Kruppel associated box zinc finger protein (KRAB-ZFP) clusters reveal that evolutionarily young KRAB-ZFPs play a redundant role in retrotransposon restriction in mice.
Madhusudhan Srinivasan, Marco Fumasoni ... Kim A Nasmyth
Sister chromatid cohesion is established during replication by two independent pathways operating in parallel, one converts chromosomal cohesin into cohesive structures while the other loads cohesin onto nascent DNAs.
Raeann Goering, Laura I Hudish ... J Matthew Taliaferro
The molecular determinants for neuronal subcellular RNA transport by FMRP are defined, with interactions between RNA G-quadruplexes and the RGG domain of FMRP being of critical importance.
