Telomere length is linked to DNA replication, and we found excessive telomeric origin firing caused by several distinct mutants did not lengthen telomeres, narrowing possible mechanisms.
Jan Hendrik van Weerd, Rajiv A Mohan ... Vincent M Christoffels
Multiple variant transcriptional regulatory elements in >1 megabasepairs of noncoding DNA close to TBX3 affect gene expression and atrioventricular conduction system function in vivo.
Interactions of eIF4A and eIF4E with specific amino-acids in the N-terminal domain of DEAD-box helicase Ded1 enhance bulk polysome assembly and translation of reporter mRNAs with structured leaders in vivo.
Reconstitution of DNA loop extrusion in cellular contexts using Xenopus egg extracts shows that condensin extrudes DNA loops non-symmetrically in metaphase, whereas cohesin extrudes DNA loops symmetrically in interphase.
Alisha Schlichter, Margaret M Kasten ... Bradley R Cairns
A SWI/SNF-family chromatin remodeling subcomplex from yeast is identified (RSC1) that is specialized to slide nucleosomes residing on DNA sequences that confer partial nucleosome unwrapping, with assistance from accessory factors.
Edward J Banigan, Aafke A van den Berg ... Leonid A Mirny
Seemingly contradictory findings of single-molecule and in vivo experiments on a major mechanism of chromosome organization are reconciled by computationally investigating mechanisms of loop extrusion that are consistent with both.
Unbiased ChIP-seq screens and genetic knockouts of large Kruppel associated box zinc finger protein (KRAB-ZFP) clusters reveal that evolutionarily young KRAB-ZFPs play a redundant role in retrotransposon restriction in mice.
