The exogenous DNA DSBs improve meiotic chromosome pairing in mouse inter-subspecific hybrids, thus providing an evidence for a DSB-dependent mechanism of the PRDM9-controlled synapsis failure and infertility.
Genome-wide recruitment of Mediator and Pol II is reduced in yeast lacking the Med2-Med3-Med15 tail module triad, and Mediator association with gene promoters depends on Pol II, Taf1, and TBP.
The pervasive transcription environment alters the efficiency of firing of replication origins, which are 'protected' by roadblock termination due to origin recognition complex (ORC) and pre-RC complexes.
Epigenetic restriction of herpes simplex virus occurs in a biphasic manner, in which ATRX maintains viral heterochromatin after an initial phase of chromatin deposition.
Mustafa Mir, Michael R Stadler ... Michael B Eisen
The probability of transcription factors binding to their target sites is choreographed through the formation of dynamic multi-protein hubs that transiently interact with actively transcribing genes.
Ilaria Pettinati, Pawel Grzechnik ... Christopher J Schofield
MBLAC1 is an endoribonuclease specific for replication dependent histone pre-mRNA processing during S-phase and may represent a new type of cancer target.
An eukaryotic specific system cooperates with the ribosome quality control pathway to maintain the reading frame during translation of slowly translated sequences.
Single-molecule experiments reveal that plectonemic supercoils occupy specific positions on DNA and a physical model relates this to the intrinsic curvature, providing an insight into how supercoiling organizes the genome.
Dhh1, Pat1, and Lsm1 target subsets of cellular mRNAs for decapping via interactions of these regulatory proteins with the C-terminal domain of Dcp2, the catalytic component of the decapping enzyme.
Eukaryotic mitochondrial RNA polymerases cap RNA with NAD with much higher efficiencies than nuclear RNA polymerase II; as a consequence, mitochondrial RNAs have remarkably high levels of NAD capping.
