Light absorption by the algal transcription factor Aureochrome 1a causes dimerization at the light-oxygen-voltage (LOV) sensing domain, which has implications for the design of synthetic photoreceptors for optogenetics.
Christina M Zimanyi, Percival Yang-Ting Chen ... Catherine L Drennan
Substrate and effector bound structures of a bacterial ribonucleotide reductase reveal the molecular basis by which substrate preference is modulated by a distal site on the enzyme.
A masked cryo-EM image classification approach and the structure of an inhibitor-bound complex provide insights into the molecular flexibility of the catalytic subunit of gamma-secretase.
A single-molecule approach is able to discriminate the redundant binding conformations of cellulosomal dockerin based on differences in their mechanical properties.
C Keith Cassidy, Benjamin A Himes ... Peijun Zhang
An atomic model of the bacterial chemosensory array obtained through the synthesis of cryo-electron tomography and large-scale molecular-dynamics simulations reveals a new kinase conformation during signaling events.
X-ray crystallography reveals that the Dna2 nuclease-helicase contains a long tunnel through which single-stranded DNA threads, and an allosteric mechanism for displacing the DNA-binding protein Rpa that restricts cleavage to the proper polarity.
Katja Gotthardt, Mandy Lokaj ... Alfred Wittinghofer
The ciliary G-protein Arl13B – which is often mutated in Joubert syndrome – is the Guanine nucleotide exchange factor for the G-protein Arl3 and exclusively localizes to cilia.
Daniel A Keedy, Lillian R Kenner ... James S Fraser
The conformations of the enzyme cyclophilin A that are essential for its catalytic activity are temperature dependent and exhibit diverse responses, which is consistent with a complex energy landscape.
