Browse our latest Structural Biology and Molecular Biophysics articles

The structure of the yeast HOPS tethering complex suggests how this large complex may catalyze fusion by tethering Rab-decorated membranes and promoting the assembly of SNAREs.

The DCC-SMLM algorithm allows to determine protein oligomeric states in situ, using the information obtained from the colocalization of fluorescent markers, overcoming the need for extraction of proteins from cells or complicated experimental setups when using microscopy.

A combination of single molecule fluorescence with DMD simulations and disulfide mapping resolved the multistate structural landscape of the PSG supramodule from PSD-95, which explains how interdomain interactions within PSD-95 enable PDZ3 binding of the critical synaptic adhesion protein neuroligin.

Single-molecule fluorescence spectroscopy and molecular dynamics simulations illuminate the structure and dynamics of PSD-95, a protein involved in neural plasticity.

Transcriptional activity is characterized for five steroid receptors complexed with multiple ligands and it is shown that the extent of transcriptional activation in complexes is accurately described by conformational shifts in computationally-generated ensembles.

The mechanism of substrate binding cooperativity in human thymidylate synthase does not derive from millisecond dynamic interconversion between active and inactive conformations in solution, but instead results primarily from differential changes in faster side-chain motions (conformational entropy), facilitated by a disordered N-terminus.

Proximity-assisted photoactivation (PAPA) provides a new way to detect protein–protein interactions in single-molecule imaging of live cells.

The isolation of the cell membrane of single cells coupled with AFM enables the study and identification of native membrane proteins in a membrane fragment.

A new binding mode between Rabphilin 3A and SNAP-25 enables efficient SNARE complex assembly via inducing a conformation change in SNAP-25 SNARE motif.

The molecular mechanism that candidalysin uses to perforate membranes is unraveled, which opens the door to the rational design of inhibitors against candidiasis.