Recovery from sensory deprivation is slow and incomplete in adult visual cortex. In this study, we show that visual stimulation during locomotion, which increases the gain of visual responses in primary visual cortex, dramatically enhances recovery in the mouse. Excitatory neurons regained normal levels of response, while narrow-spiking (inhibitory) neurons remained less active. Visual stimulation or locomotion alone did not enhance recovery. Responses to the particular visual stimuli viewed by the animal during locomotion recovered, while those to another normally effective stimulus did not, suggesting that locomotion promotes the recovery only of the neural circuits that are activated concurrent with the locomotion. These findings may provide an avenue for improving recovery from amblyopia in humans.https://doi.org/10.7554/eLife.02798.001
Amblyopia, otherwise known as ‘lazy eye’, is a condition in which vision fails to develop normally during childhood, not due to problems with the eye itself but due to problems with the transmission of information from the eye to the brain. It occurs when disorders such as squint—in which the eyes point in different directions—cause the brain to continually ignore input from one eye, with the result that vision in that eye never fully develops.
If detected in infancy, amblyopia can be treated by surgery, although such interventions must be performed early because they are much less effective when used on adults. However, Kaneko and Stryker now present data suggesting that the adult mammalian visual system may be more amenable to change than previously thought.
Young mice were deprived of visual input to one eye, by having an eyelid sewn shut, during a critical period in the development of their visual systems. When the eye was re-opened at the age of 4–5 months, the mice showed reduced responses in the brain region corresponding to that eye. However, if the mice were then allowed to run on a treadmill for several hours a day while viewing a visual stimulus—either black and white gratings or random noise—the vision in their deprived eye showed a rapid and striking improvement. This improvement was not seen in mice that ran without a visual stimulus, or in mice that looked at the visual stimulus but did not run. Moreover, the improvement was specific to the particular stimulus viewed whilst running.
Although the mechanism behind this effect is unclear, it is known that running increases neuronal activity, and one possibility is that neurons that are active simultaneously—such as those encoding the visual stimulus—form stronger connections with one another: ‘neurons that fire together, wire together’. Further work is required to determine whether similar changes occur in the human visual system and, if they do, whether they could be applied to the treatment of amblyopia.https://doi.org/10.7554/eLife.02798.002
Depriving one eye of normal patterned vision during early life causes a loss of visual acuity (amblyopia) and neurons in the visual cortex lose responsiveness through the deprived eye. If such abnormal vision is left uncorrected, recovery of visual function in adulthood is slow and incomplete, both in humans and in higher mammals, reflecting the limited plasticity of mature cortex (Hubel and Wiesel, 1970; Mitchell and Sengpiel, 2009). The recent discovery that neurons in mouse visual cortex respond with more than two times the number of action potentials during active locomotion than when still (Niell and Stryker, 2010) led us to test whether this enhanced response might facilitate recovery from the effects of early-onset, long-term monocular visual deprivation (MD).
Monocular deprivation was initiated by suturing shut the right eyelid of C57BL/6 mice early in the critical period (P22–24) and was continued to 4 to 5 months of age. The right eye was then re-opened to allow for binocular vision (BV), and baseline cortical responses through the two eyes were recorded using intrinsic signal imaging. Both eyes remained open afterward, and changes in responsiveness were measured over the next 3 weeks (Figure 1A). For 4 hr each day during these 21 days of BV (21d-BV), experimental animals viewed a visual stimulus (VS) while being permitted to run on a freely rotating spherical treadmill with their heads fixed. We used contrast-modulated stochastic noise matched to the spatiotemporal frequency response of the mouse as the visual stimulus because it drives nearly all cells in the primary visual cortex to some extent (Niell and Stryker, 2008).
In control mice that were kept in the standard housing condition (home-cage), cortical responses to the closed eye in binocular visual cortex slowly increased over 21d-BV, at which point they were still well below the range of age-matched mice with normal visual experience (Figure 1B,D). In contrast, mice that experienced the noise stimulus during their daily running (VS+run) showed a remarkable increase in closed-eye responses after only 7 days of BV (Figure 1C,D). Examined in a separate set of animals, this effect of VS+run was already significant after 3 days of training (Figure 1—figure supplement 1). Responses through the open eye did not change significantly in either group (Figure 1B,C,E). As a result, the ocular dominance index (ODI), computed as a normalized difference between responses to two eyes, recovered much more rapidly in animals with visual experience during running than in controls (Figure 1F). Recovery using reverse occlusion (switching the eye closure) instead of BV was similarly enhanced by VS+run (Figure 1—figure supplement 2).
To determine whether VS or running alone enhances recovery, we tested two more groups of mice: one that ran on the ball without VS (run-only) and one that viewed the visual noise stimulus in the home cage but did not run on the ball (VS-only). Closed-eye responses and ODI in both of these groups increased only slowly, similar to those of the home-cage control group (Figure 1G,I). Running velocity and duration were similar between run-only and VS+run groups (Figure 1—figure supplement 3).
VS+run enhanced recovery of monocular visual areas as well (Figure 2). This effect was rapid (Figure 2—figure supplement 1) and was particularly prominent for a secondary monocular area, where the home cage and the run-only groups showed no significant improvement even after 21 days of BV.
Perception in humans can be strengthened by training in an experience-specific manner (reviewed in Sagi, 2011), and responses in mouse visual cortex are reported to do so as well (Frenkel et al., 2006). We next tested whether recovery of closed-eye responses is preferentially enhanced to the particular visual stimuli presented during running. Mice viewed either square bars drifting in eight evenly-spaced directions (barVS) or the contrast-modulated noise (noiseVS) during running each day for 3 weeks. Intrinsic signal responses to both bar and noise stimuli were measured weekly. Mice that experienced barVS+run showed significantly greater recovery of closed-eye responses to bars than to noise (Figure 3). Likewise, mice that experienced noiseVS+run showed significantly greater recovery of response to noise than to bars (Figure 3). Responses to the stimulus that was not experienced during running were similar to those in control mice presented with a blank screen during running. These observations reveal that enhanced recovery is stimulus-specific and suggest that only the specific visual cortical circuits that are active during locomotion recover.
To reveal changes in response properties of individual neurons, we made extracellular single unit recordings from neurons in layers 2/3 and 4 of binocular V1 contralateral to the closed eye. The use of 16-site silicon probes and spike-sorting methods (Niell and Stryker, 2008) enabled unbiased sampling from both responsive and unresponsive units. We examined five groups of mice with different treatments (Figure 4A). We first describe results from the broad-spiking, presumed excitatory cells that constitute ∼80% of the recordings.
Consistent with the intrinsic signal observations as described above, closed-eye responses in mice that viewed visual stimuli while running during 7d-BV recovered significantly from their profoundly decreased level after long-term monocular deprivation (LTMD), in a stimulus-specific manner (Figure 4, Figure 4—figure supplements 1, 2). Closed-eye responses to the optimal grating (preferred orientation and spatial frequency) were most increased in grating+run mice among groups (Figure 4B), while responses to contrast-modulated noise were improved most in noise+run mice (Figure 4C). Open-eye responses did not differ among four groups of monocularly deprived mice (Figure 4—figure supplement 1). As a result, ocular dominance recovered to normal levels with similar stimulus specificity (Figure 4B,C).
In normal animals, the majority of cells in layers 2–4 are orientation-selective (OSI >0.5, Figure 5A,B) and the OSI for the two eyes is similar in each binocular cell (Figure 5—figure supplement 1B). Closed-eye OSIs and the binocular correlation of OSI were significantly improved only in grating+run mice but not in other groups (Figure 5A,B, Figure 5—figure supplement 1B). Prolonged MD caused mismatch of the preferred orientation of two eyes (Figure 5C,D), consistent with requirement of binocular visual experience during the critical period to develop orientation matching (Wang et al., 2010). 7d-BV improved binocular matching almost to normal levels in grating+run but not in home-cage or noise+run, mice (Figure 5C,D). In addition, the distribution of the preferred spatial frequency in closed-eye responses was almost normalized in grating+run mice but not in home-cage or noise+run mice. (Figure 5E,F). Such shifts in preferred spatial frequency may account for improvements in behaviorally measured acuity (Mitchell and Sengpiel, 2009).
In response to the contrast-modulated noise stimuli, the peak firing of a cell occurs at the frequency of contrast modulation (F1, at 0.1 Hz). We assessed contrast sensitivity by calculating average value of contrast that elicits half-maximal response (C1/2). Contrast sensitivity was significantly impaired after LTMD and was restored almost completely in noise+run mice but not in gratings+run mice (Figure 6).
Narrow-spiking cells are thought to correspond to inhibitory, predominantly fast-spiking, interneurons (McCormick et al., 1985; Bartho et al., 2004). Although a minority population, inhibitory cells play an important role in plasticity (Espinosa and Stryker, 2012). We observed several notable differences between broad- and narrow-spiking cells in the changes following LTMD and 7d-BV. First, spontaneous activity of narrow-spiking cells was greatly reduced after LTMD and did not change significantly after 7d-BV regardless of the treatment (Figure 7C,D), whereas those of broad-spiking cells were elevated after LTMD and significantly decreased toward normal level in mice after 7d-BV (Figure 7A,B). These opposite changes in spontaneous firing between broad- and narrow-spiking cells may reflect a homeostatic mechanism that alters excitatory–inhibitory balance to maintain cortical activity during prolonged deprivation. Second, recovery of closed-eye responses and ocular dominance to the gratings in narrow-spiking cells was only modest even in grating+run mice (Figure 8A, Figure 8—figure supplement 1A), whereas it was nearly complete in broad-spiking cells (Figure 4B). Third, responses of narrow-spiking cells to contrast-modulated noise through the deprived eye and ocular dominance were incompletely restored both in noise+run and grating+run mice to similar extents, showing no preference for the experienced stimulus (Figure 8B, Figure 8—figure supplement 1B). Fourth, responses of narrow-spiking cells through the open-eye were not elevated after LTMD either to the noise or grating stimulus, and did not change significantly in any of 7d-BV mice (Figure 8—figure supplement 1A,B). This lack of potentiation of open-eye responses during LTMD in narrow-spiking cells may contribute to potentiation of those in broad-spiking cells. The responses of narrow-spiking cells after LTMD suggest that deprivation reduces intracortical inhibition. Such a reduction may provide a starting point that allows meager deprived-eye excitatory pathways of the cortical circuit to drive activity upon re-opening. The incomplete recovery of orientation tuning of broad-spiking cells in spite of nearly full restoration of the magnitudes of responses may result from the very limited recovery in narrow-spiking cells, as inhibition is critical for generation of sharp orientation tuning in upper layer neurons in the primary visual cortex (Liu et al., 2011).
Recovery in adulthood from amblyopia induced by monocular visual deprivation during early life is slow and far from complete, even weeks after opening the deprived eye (Hubel and Wiesel, 1970; Mitchell and Sengpiel, 2009). In this study, we have used intrinsic signal imaging and single cell recording to find that locomotion, which is known to increase visual responses (Niell and Stryker, 2010), dramatically enhances the recovery of visual responses, almost to normal levels within a week. Surprisingly, the recovery of response was specific to the particular visual stimuli presented during locomotion. Neither locomotion alone nor visual stimulation alone promoted recovery. These findings suggest that recovery is facilitated only in the neural circuits that are activated during running.
Other manipulations have been reported to enhance adult plasticity (Bavelier et al., 2010), such as dark exposure (He et al., 2007), environmental enrichment (Sale et al., 2007), and antidepressant treatment (Maya Vetencourt et al., 2008). These treatments have in common a decrease in intracortical inhibition, consistent with our finding of reduced activity of inhibitory neurons. Such treatments may also increase locomotion, producing an active cortical state that facilitates plasticity. Interestingly, active hunting by barn owls, flying rather than walking or running in this case, dramatically increases adaptive plasticity of the auditory map in the superior colliculus (Bergan et al., 2005).
A number of mechanisms may contribute to the enhanced recovery described here. Locomotion may induce activity in neuromodulatory circuits that might enhance cortical function or plasticity (Carcea and Froemke, 2013), and it may also produce hemodynamic changes that might affect the operation of the cortical circuit (Seifert and Secher, 2011). The stimulus specificity of enhanced recovery, however, rules out any general explanation of recovery as an increase in cortical response to any stimulus. Most simply, the larger responses of the cortical neurons themselves during locomotion would be expected to enhance recovery through the operation of any Hebbian or spike-timing based plasticity mechanism that depends on neural activity (Feldman, 2009). Plasticity of excitatory connections would be further enhanced by the opposite changes in spontaneous firing of broad- and narrow-spiking neurons.
The present findings do not allow a rigorous test of the hypothesis that Hebbian changes are responsible for the stimulus specificity of recovery; i.e., that the neurons or synapses that are driven better by the specific stimuli presented during locomotion become more powerful. Such a test would require monitoring of the responses of individual cells or the efficacy of the synapses that they make throughout the recovery period. However, responses to both grating and noise stimuli were measured in three large populations of broad-spiking neurons, one before recovery and two in separate groups of animals after recovery during exposure to grating or noise. Making a correspondence between the individual neurons in these samples, in order to create fictive repeated measures from the same neurons, may suggest whether the present data are compatible with the Hebbian hypothesis. Indeed, the fictive longitudinal data show that noise stimulation increased the responses of neurons originally responding better to noise more than it did for the neurons that responded better to grating, and vice versa (Figure 4—figure supplement 2). These findings are consistent with the Hebbian hypothesis.
In human visual cortex, it is unknown whether neuronal responses or plasticity are enhanced by locomotion. In monkeys, attention modulates the gain of cortical responses in higher visual areas, but these effects are very much smaller in V1 and smaller than those of locomotion in mice (Maunsell and Cook, 2002; Reynolds and Chelazzi, 2004). It will be interesting to determine whether V1 responses in humans are enhanced by locomotion and whether recovery from amblyopia in humans can be similarly enhanced.
C57BL/6 wild-type breeders were purchased from Jackson Laboratory (Bar Harbor, ME) and bred as needed. Animals were maintained in the animal facility at University of California San Francisco and used in accordance with Protocol AN098080-01D approved by the UCSF Institutional Animal Care and Use Committee. Monocular deprivation (MD) was performed as described (Gordon and Stryker, 1996) except that 2–3% isoflurane in oxygen was used for anesthesia. The lid of the right eye was sutured shut at P22–24. Mice were housed in the standard condition (12/12 dark–light cycle, free access to food and water) until P135∼150, at which time a custom stainless steel plate for head fixation was attached to the skull with dental acrylic under isoflurane anesthesia. The exposed surface of the skull was covered with a thin coat of nitrocellulose (New-Skin, Medtech Products Inc., NY) to prevent desiccation, reactive cell growth, and destruction of the bone structure. Animals were given a subcutaneous injection of carprofen (5 mg/kg) as a post-operative analgesic. 5–7 days after head-plate implantation, the closed eyelid was re-opened and groups of mice for the intrinsic signal imaging study underwent the first imaging session (day 0). The re-opened eyelid was left open afterward to allow binocular vision while animals were subjected to the different regimes of visual stimulation and locomotion as follows. (1) running with visual stimulation: The mouse was allowed to freely move its limbs and trunk on a foam ball floated on a stream of air, while its head was fixed via the implanted headplate that could be screwed into a rigid crossbar above the floating ball as described (Niell and Stryker, 2010). Two optical mice were used to measure the displacement of the ball as the mouse moved, allowing us to calculate the physical speed of the ball (Niell and Stryker, 2010). After re-opening the deprived eye, each animal spent 4 hr daily on the ball while viewing visual stimulus, over next 3 weeks (for intrinsic signal optical imaging) or 6 days (for single unit recording). Running was performed during the dark phase of the housing cycle, because we found that mice ran for longer times during this phase. (2) running without visual stimulation: Each animal ran on the floating ball exactly as the first group but viewed only a blank, mid-gray screen (∼35 lux). (3) visual stimulation alone without running (intrinsic signal imaging only): a transparent cage containing a group of mice with regular bedding materials was surrounded by four monitors with the distance of 25 cm between the center of the cage and the monitor. The visual stimulus was presented on the monitors continuously for 7–8 hr daily during which animals were allowed to behave freely without disruption by the experimenter. We extended the duration to 7–8 hr because animals were obviously active only 50–60% of time. (4) no running, no visual stimulation: mice were housed in the standard condition without running or specific visual stimulation. (5) Non-deprived control for single unit recording only: age-matched control animals, many of them being in the same litter as those in experimental groups, were housed in the standard condition with normal visual experience without running or specific visual stimulation.
The daily visual stimulus for animals used in intrinsic signal imaging was either contrast-modulated noise movies or drifting square bars at the full range of orientations. The stimulus for mice employed in single unit recordings was either the noise or drifting sinusoidal gratings. Stimuli were generated in Matlab using Psychophysics Toolbox extensions (Brainard, 1997; Pelli, 1997), and displayed on a LCD monitor (60-Hz refresh rate) placed 25 cm from the mouse, spanning 52.5° (height) × 70° (width) of visual space. The drifting bar was full-length of the monitor, width of 2°, velocity of 25°/s, and drifting in eight equally spaced directions. The drifting sinusoidal gratings were shown for a duration of 1.5 s, at temporal frequency of 2 Hz, spatial frequency of 0.01, 0.02, 0.04, 0.08, 0.16, 0.32, and 0 (full-field flicker) cycles/° (cpd), in 12 equally spaced directions. The contrast-modulated Gaussian noise movie consisted of the Fourier-inversion of a randomly generated spatiotemporal spectrum with low-pass spatial and temporal cutoffs applied at 0.05 cpd and 4 Hz, respectively. To provide contrast modulation, the movie was multiplied by a sinusoid with a 10-s period. Movies were generated at 60 ×60 pixels and then smoothly interpolated by the video card to 480 × 480 to appear 30 × 30 cm on the monitor and played at 30 frames per second. Each movie was 5 min long and repeated for 4 hr total presentation.
5–7 days after the headplate implantation, the first imaging of intrinsic signals was performed to measure baseline responses through each eye. The mouse was anesthetized with isoflurane (3% for induction and 0.7% during recording) supplemented with intramuscular injection of chlorprothixene chloride (2 µg/g body weight), and the closed eyelid was carefully opened by slitting horizontally at the center of the fused lid just before the imaging session. Repeated optical imaging of intrinsic signals was performed as described (Kaneko et al., 2008). We monitored the concentration of isoflurane using an Ohmeda 5250 RGM (Datex-Ohmeda, Madison, WI) throughout each imaging session. Images were recorded transcranially through the window of the implanted headplate. Intrinsic signal images were obtained with a Dalsa 1M30 CCD camera (Dalsa, Waterloo, Canada) with a 135 × 50 mm tandem lens (Nikon Inc., Melville, NY) and red interference filter (610 ± 10 nm). Frames were acquired at a rate of 30 fps, temporally binned by four frames, and stored as 512 × 512 pixel images after binning the 1024 × 1024 camera pixels by 2 × 2 pixels spatially. We used two kinds of visual stimuli presented between −5° and 15° (azimuth) on the stimulus monitor (0° = center of the monitor aligned to center of the mouse) to record intrinsic signals in the binocular visual cortex: (1) 2°-wide bars, moving continuously and periodically upward or downward at a speed of 10°/sec; (2) the contrast-modulated noise movie, as described above. To record in the monocular visual area, the contrast-modulated noise was presented between 50° and 70° (azimuth) of the visual field. Visual stimuli were presented on a 40 × 30 cm monitor placed 25 cm in front of the mouse. The phase and amplitude of cortical responses at the stimulus frequency were extracted by Fourier analysis as described (Kalatsky and Stryker, 2003). Response amplitude was an average of at least four measurements. Ocular dominance index was computed as (R − L)/(R + L), where R and L are the peak response amplitudes through the right eye and the left eye, respectively, as described (Kaneko et al., 2008). All mice were kept under standard housing conditions with free access to food and water between recordings and daily running on the treadmill.
Data acquisition, visual stimuli, and spike analyses were performed as described (Niell and Stryker, 2008) with minor modifications. Briefly, after locating the binocular area of the primary visual cortex by recording intrinsic signals elicited by ipsilateral eye stimulation, a small craniotomy (∼2 mm diameter) was made over the binocular area through the window of the implanted headplate. A silicon multisite electrode with a tetrode configuration (model a2 × 2-tet-3mm-150-121, Neuronexus Technologies, MI) was inserted to a depth of <400 μm below the cortical surface to record cells in layer 2/3 and 4. Visual stimuli were generated in Matlab using the Psychophysics Toolbox extensions and displayed on a monitor placed 25 cm in front of the mouse as described above. In each animal in all treatment groups, the responsiveness of cells was measured using both drifting sinusoidal gratings and the contrast-modulated noise movie, as described above. Neuronal signals (spikes) were acquired using a System 3 workstation (Tucker–Davis Technologies, FL) and analyzed with custom software in Matlab (MathWorks, MA). Single units were identified by clustering spike waveforms using the FastICA Matlab package and KlustaKwik (Harris et al., 2000). Units were classified as broad- or narrow-spiking based on trough-to-peak time and the slope of the waveform 0.5 ms after the initial trough as described (Niell and Stryker, 2008).
Drifting sinusoidal gratings as described above (2.5 min/set) were repeated six times for each eye (30 min total). The average spontaneous firing rate for each unit was calculated by averaging the rate over all blank condition presentations. Responses to each orientation and spatial frequency were calculated by averaging the spike rate during the 1.5 s presentation and subtracting the spontaneous firing rate. For assessing ocular dominance, we first selected units responsive to the optimal grating (≥ 2.0 spikes/s) through either eye and then calculated ODI for each unit as the difference between the contralateral (deprived) and ipsilateral (open) eyes divided by the sum of the two eyes' responses (Kaneko et al., 2008).
The preferred orientation was determined by averaging the response across all spatial frequencies, and calculating half the complex phase of the value . Given this fixed preferred orientation θpref, the tuning curve was fitted with the sum of two Gaussians centered on θpref and θpref + π, of different amplitudes A1 and A2 but equal width θ, with a constant baseline B. From this fit, we derived an orientation selectivity index (OSI) and the width of the selectivity tuning. OSI was calculated as the depth of modulation from the preferred orientation to its orthogonal orientation θortho = θpref + π /2, as (Rpref − Rortho)/(Rpref + Rortho). Tuning width was the half-width at half-maximum of the fit above the baseline. The difference in preferred orientation between the two eyes was calculated by subtracting ipsilateral from contralateral preferred orientation along the 180° cycle (−90° ∼ +90°). The absolute values of these differences were used in statistical analyses. The spatial frequency tuning curve was determined from the response at the preferred orientation (θpref) described above and was fit to a difference of two Gaussians.
We probed the overall responsiveness of cells using movies of stochastic noise with defined spatial and temporal frequency spectra. The contrast of movies was periodically modulated so that each movie transitioned sinusoidally from a gray background to full contrast movie and back to gray again, with a 10-s period. This generally resulted in a periodic modulation of firing and also helped to maintain high firing rates throughout the presentation by preventing habituation. The movie was shown for 10–15 min for each eye, and the cells' responsiveness was presented as the response amplitude at the frequency of the contrast modulation (F1 response, the first harmonic at 0.1 Hz). A form of contrast–response curve was generated by plotting the averaged response over all cycles to the contrast. Average value of the contrast that elicits half-maximal response (C1/2) was used to measure contrast sensitivity.
Data were presented as mean ± SEM or median ± SE in the main text, as well as cumulative frequency distributions in the supplemental figures, unless otherwise indicated. Standard error of the median was calculated by a bootstrap. Response magnitudes and ocular dominance index data obtained from intrinsic signal imaging experiments were analyzed by using repeated measures ANOVA to assess changes from baseline as well as differences between treatments, which were followed by multiple comparisons with Bonferroni correction. Electrophysiological single unit data were analyzed using either one-way ANOVA (for normally distributed data) or Kruskal–Wallis one-way ANOVA (for the rest), followed by Bonferroni or Dunn multiple comparisons between treatments. Difference in cumulative frequency distribution was analyzed using the Kolmogorov–Smirnov test. These statistical analyses were performed using Prism 5 (GraphPad Software, CA) or Matlab (MathWorks, MA).
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Sacha B NelsonReviewing Editor; Brandeis University, United States
eLife posts the editorial decision letter and author response on a selection of the published articles (subject to the approval of the authors). An edited version of the letter sent to the authors after peer review is shown, indicating the substantive concerns or comments; minor concerns are not usually shown. Reviewers have the opportunity to discuss the decision before the letter is sent (see review process). Similarly, the author response typically shows only responses to the major concerns raised by the reviewers.
Thank you for sending your work entitled “Exercise promotes recovery of function in adult visual cortex” for consideration at eLife. Your article has been favorably evaluated by Eve Marder (Senior editor) and 2 reviewers, one of whom, Sacha Nelson, is a member of our Board of Reviewing Editors.
The Reviewing editor and the other reviewer discussed their comments before we reached this decision, and the Reviewing editor has assembled the following comments to help you prepare a revised submission.
The present study is a rigorous examination of the impact of locomotion and visual stimulation on the recovery of mice from amblyopia. The authors make the surprising and interesting (and potentially clinically important) discovery that pairing visual stimulation with the animal's own locomotion dramatically improves the responses through the eye that underwent long-term deprivation. Further, the authors go further to demonstrate that major forms of selectivity, including orientation and spatial frequency selectivity, are dramatically improved by the new protocol compared to locomotion or visual stimulation alone (or home-cage experience).
The reviews raised two major issues, both of which should be quite straightforward to deal with.
1) One feature of the results would benefit from clarification perhaps requiring additional analysis. The authors find that responses to a noise stimulus or bar stimulus are selectively enhanced by prior exposure to the same stimulus while running. They suggest that a Hebbian synaptic plasticity model may account for these results. This predicts that to the extent that the enhancement is stimulus specific, the stimuli should activate different circuits. One might imagine, for example, that the selective enhancement would be especially pronounced for cells that respond much better to one stimulus than the other, while cells that respond to both stimuli equally might be expected to show more balanced enhancement (although this need not be the case if the two stimuli activate the postsynaptic neuron through separate sets of synapses). Because the authors have recorded responses to both sets of stimuli in many individual cells they should be able to address this question of whether selective enhancement depends on selective response. This would potentially strengthen the interpretation in terms of an underlying plasticity mechanism. Although this analysis may be complicated by differences in the responses to gratings vs. bars, these differences are likely less prominent and presumably the authors have some handle on how these responses differ.
2) In the Title and Abstract, the authors refer to locomotion or running with the more generic term “exercise”. We appreciate that the authors are going for big picture appeal, but we are concerned that many readers will insert their own interpretation for “exercise”. In fact, the findings the authors report are related to a specific activity, locomotion/running for an extended period of time (several hours). In our opinion, the authors should use a specific term such as “running” or “locomotion”. The paper is very clear and careful; it would be shame for the conclusions to be misapplied by someone who only looks at the Title/Abstract. (Maybe “exercise” as a keyword?)https://doi.org/10.7554/eLife.02798.023
Thank you for the reviews of our manuscript. We have carried out the analysis suggested as major issue #1 and we have incorporated new text in the manuscript, a new paragraph in the Discussion, and a new supplemental figure (Figure 4–figure supplement 2) to address the conclusions that are sustained by this analysis. Following the suggestion outlined in the reviewers’ major issue #2, we have also revised the manuscript title and excised the word ‘exercise’ from our text at several points.https://doi.org/10.7554/eLife.02798.024
- Michael P Stryker
- Megumi Kaneko
The funders reviewed the study design as a specific aim of a grant application but had no role in data collection and interpretation or the decision to submit the work for publication.
This work was supported by NIH grant R01 EY02874 and T32 MH089920. We thank the members of the Stryker laboratory for critical reading, Cristopher Niell for assistance with microelectrode recording, and SW Zucker for suggesting the earth mover distance algorithm.
Animal experimentation: This study was performed in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. All of the animals were handled according to protocols approved by the UCSF institutional animal care and use committee under IACUC Protocol AN098080-01D.
- Sacha B Nelson, Brandeis University, United States
- Received: March 14, 2014
- Accepted: May 26, 2014
- Version of Record published: June 26, 2014 (version 1)
© 2014, Kaneko and Stryker
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
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Viewing a visual stimulus whilst running helps mice that have lost their sight in one eye to make a near-complete recovery.
Internal state alters sensory behaviors to optimize survival strategies. The neuronal mechanisms underlying hunger-dependent behavioral plasticity are not fully characterized. Here we show that feeding state alters C. elegans thermotaxis behavior by engaging a modulatory circuit whose activity gates the output of the core thermotaxis network. Feeding state does not alter the activity of the core thermotaxis circuit comprised of AFD thermosensory and AIY interneurons. Instead, prolonged food deprivation potentiates temperature responses in the AWC sensory neurons, which inhibit the postsynaptic AIA interneurons to override and disrupt AFD-driven thermotaxis behavior. Acute inhibition and activation of AWC and AIA, respectively, restores negative thermotaxis in starved animals. We find that state-dependent modulation of AWC-AIA temperature responses requires INS-1 insulin-like peptide signaling from the gut and DAF-16 FOXO function in AWC. Our results describe a mechanism by which functional reconfiguration of a sensory network via gut-brain signaling drives state-dependent behavioral flexibility.