Salmonella Typhi and Salmonella Paratyphi A elaborate distinct systemic metabolite signatures during enteric fever
- Umeå University, Sweden
- The Hospital for Tropical Diseases, Vietnam
- Patan Academy of Health Sciences, Nepal
- The Hospital for Tropical Diseases, Nepal
Abstract
The host-pathogen interactions induced by Salmonella Typhi and Paratyphi A during enteric fever are poorly understood. This knowledge gap, and the human restricted nature of these bacteria, limit our understanding of the disease and impede the development of new diagnostic approaches. To investigate metabolite signals associated with enteric fever we performed mass spectrometry (GCxGC/TOFMS) on plasma from patients with Typhi and Paratyphi A infections and controls, identifying 695 individual metabolite peaks. Applying supervised pattern recognition, we found highly significant and reproducible metabolite profiles separating Typhi cases, Paratyphi A cases, and controls, calculating that a combination of six metabolites could define the etiological agent. For the first time we show that reproducible and serovar specific systemic biomarkers can be detected during enteric fever. Our work defines several biologically plausible metabolites that can be used to detect enteric fever, and unlocks the potential of this method in diagnosing other systemic bacterial infections.
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Ethics
Human subjects: The institutional ethical review boards of Patan Hospital and The Nepal Health Research Council and the Oxford Tropical Research Ethics Committee in the United Kingdom approved this study. All adult participants provided written informed consent for the collection and storage of all samples and subsequent data analysis, written informed consent was given for all those under 18 years of age by a parent or guardian (Arjyal et al. 2011).
Copyright
© 2014, Näsström et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Salmonella Typhi and Salmonella Paratyphi A elaborate distinct systemic metabolite signatures during enteric fever
eLife 3:e03100.
https://doi.org/10.7554/eLife.03100
Further reading
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- Epidemiology and Global Health
- Microbiology and Infectious Disease
The use of metabolomics could lead to improved diagnostics for enteric fever.
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- Immunology and Inflammation
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