Quantitative analysis of mammalian GIRK2 channel regulation by G proteins, the signaling lipid PIP2 and Na+ in a reconstituted system

  1. Weiwei Wang
  2. Matthew R Whorton
  3. Roderick MacKinnon  Is a corresponding author
  1. Howard Hughes Medical Institute, The Rockefeller University, United States


GIRK channels control spike frequency in atrial pacemaker cells and inhibitory potentials in neurons. By directly responding to G proteins, PIP2 and Na+, GIRK is under the control of multiple signaling pathways. In this study, the mammalian GIRK2 channel has been purified and reconstituted in planar lipid membranes and effects of Gα, Gβγ, PIP2 and Na+ analyzed. Gβγ and PIP2 must be present simultaneously to activate GIRK2. Na+ is not essential but modulates the effect of Gβγ and PIP2 over physiological concentrations. Gαi1(GTPγS) has no effect, whereas Gαi1(GDP) closes the channel through removal of Gβγ. In the presence of Gβγ, GIRK2 opens as a function of PIP2 mole fraction with Hill coefficient 2.5 and an affinity that poises GIRK2 to respond to natural variations of PIP2 concentration. The dual requirement for Gβγ and PIP2 can help to explain why GIRK2 is activated by Gi/o, but not Gq coupled GPCRs.


eLife digest

Though every cell in the body is surrounded by a membrane, there are a number of ways that molecules can pass through this membrane to either enter or leave the cell. Proteins from the GIRK family form channels in the membranes of mammalian cells, and when open these channels allow potassium ions to flow through the membrane to control the membrane's voltage.

GIRK channels are found in the heart and in the central nervous system, and can be activated in a variety of ways. Sodium ions and molecules called ‘signaling lipids’ can regulate the activation of GIRK channels. These channels can also be caused to open by G proteins: proteins that are found inside cells and that help to transmit signals from the outside of a cell to the inside. Three G proteins—called Gα, Gβ, and Gγ—work together in a complex that functions a bit like a switch. When switched on, the Gα subunit is separated from the other two subunits (called Gβγ); and both parts can then activate different signaling pathways inside the cell.

The Gβγ subunits and a signaling lipid have been known to regulate the opening of GIRK channels for a number of years, but these events have only been studied in the context of living cells. The specific role of each molecule, and whether the Gα subunit can also regulate the GIRK channels, remains unknown. Now Wang et al. have produced one type of mouse GIRK channel, called GIRK2, in yeast cells, purified this protein, and added it into an artificial membrane. This ‘reconstituted system’ allowed the regulation of a GIRK channel to be investigated under more controlled conditions than in previous experiments.

Wang et al. found that the Gβγ subunits and the signaling lipid both need to be present to activate the GIRK2 channel. Sodium ions were not essential, but promoted further opening when Gβγ and the signaling lipid were already present. When locked in its ‘on’ state, the Gα subunit had no effect on GIRK2, but adding Gα locked in the ‘off’ state closed these channels by removing the Gβγ proteins.

The findings of Wang et al. suggest that it should be possible to use a similar reconstituted system to investigate what allows different G proteins to activate specific signaling pathways.



G protein gated inward rectifier K+ (GIRK) channels are inward rectifier K+ (Kir) channels whose activity is regulated by GTP binding proteins (G proteins) (Brown and Birnbaumer, 1988; Yamada et al., 1998). They are present in many different cell types including electrically excitable cells in the cardiovascular and nervous systems (Dascal et al., 1993; Lesage et al., 1994; Krapivinsky et al., 1995; Lesage et al., 1995; Karschin et al., 1996). GIRK channels regulate cellular electrical activity by modulating K+ conductance near the resting membrane potential. Specifically, stimulation of certain G protein coupled receptors (GPCRs) on a cell's surface leads to the opening of GIRK channels, which increases K+ conductance and opposes the initiation of action potentials. This inhibitory effect on excitation is best understood in the cardiovascular system, where vagus nerve stimulation slows heart rate through acetylcholine action on GPCRs in atrial pacemaker cells (Loewi and Navratil, 1926; Giles and Noble, 1976; Osterrieder et al., 1981). The ensuing activation of GIRK channels prolongs the interval between pacemaker action potentials, thus causing the heart rate to slow. GIRK channels are also abundant in the nervous system, where they modulate slow inhibitory postsynaptic currents in response to neurotransmitter stimulation (Luscher et al., 1997; Lujan et al., 2009).

Cellular electrophysiology experiments and biochemical analysis have yielded a rich understanding of GIRK channel function. As background to the questions addressed in the present study, the following conclusions have been established over the past several decades of study. First, the G protein ‘subunit’ called Gβγ (a tightly bound complex of β and γ protein polypeptides) is the major mediator through which GPCR stimulation opens GIRK channels (Pfaffinger et al., 1985; Logothetis et al., 1987; Reuveny et al., 1994; Wickman et al., 1994; Huang et al., 1995; Inanobe et al., 1995; Kofuji et al., 1995; Krapivinsky et al., 1995). When a GPCR is stimulated through the binding of a neurotransmitter (or drug) on the outside of the cell membrane it catalyzes guanine nucleotide exchange–GTP replaces GDP on the Gα subunit–and release of Gα(GTP) and Gβγ subunits on the cytoplasmic side (Gilman, 1987). These subunits are then free to activate their ‘target’ proteins: Gβγ activates GIRK. Second, the signaling lipid phosphatidylinositol 4,5-bisphosphate (PIP2) also regulates GIRK channel opening (Huang et al., 1998; Sui et al., 1998; Logothetis and Zhang, 1999). This property means that GIRK channels respond to more than one ligand and thus are under the control of more than one distinct signaling pathway. Third, the activity of certain GIRK channels, for example the neuronal GIRK2 channel, is also regulated by the concentration of intracellular Na+ (Petit-Jacques et al., 1999; Ho and Murrell-Lagnado, 1999a, 1999b). Intracellular Na+ concentration increases during excessive electrical excitation. Activation of GIRK2 under this circumstance may serve as a cell protective mechanism through action potential suppression. Finally, x-ray crystallography has recently provided atomic level descriptions of a neuronal GIRK channel, GIRK2, in the absence and presence of Gβγ, PIP2 and Na+ (Whorton and MacKinnon, 2011, 2013).

The present study addresses the following still unanswered questions. First, does the Gα(GTP) subunit also regulate GIRK function? The Gα subunit co-precipitates with GIRK in biochemical ‘pull-down’ experiments (Huang et al., 1995; Peleg et al., 2002; Ivanina et al., 2004; Rubinstein et al., 2009; Berlin et al., 2010, 2011) and also exhibits interaction with the channel's cytoplasmic domain as detected by solution NMR spectroscopy (Mase et al., 2012). Do these interactions underlie channel regulation by Gα? Second, how does GIRK channel activity depend on the concentration of PIP2 in the membrane? PIP2 is a signaling lipid whose concentration in the cell membrane changes (Lemmon, 2008; Balla, 2013). To know whether GIRK activity is responsive to these changes, a quantitative description of the channel's PIP2 concentration dependence is needed. Until now, no method has existed to change PIP2 in a known, quantitative and controlled manner in membranes in which GIRK channel activity is measured. Third, do Gβγ, PIP2 and Na+, act on the channel independently or do they function together? The issue of multi-ligand dependence is relevant to understanding the molecular biophysics of channel regulation and to understanding how multiple signaling pathways control membrane potential. At the level of molecular biophysics we wish to understand how multiple allosteric inputs regulate channel opening. At the level of cellular control of membrane potential we wish to understand GIRK's AND/OR relationship with respect to multiple signaling inputs.

The real difficulty in answering the above questions lies in the compositional complexity and uncertainty of living cell membranes—the natural system in which nearly all past experiments have been carried out. In this study we use the planar lipid bilayer technique to characterize the regulation of GIRK channels by G-proteins, PIP2 and Na+ ions. The bilayer technique is a total reconstitution method in which the channel and its various regulatory components are first purified to homogeneity and then combined. Every component of the system is known to within a small error in composition and amount, including the membrane, which is produced from either purified or synthetic lipids.


Robust GIRK2 activity depends on the presence of both Gβγ and PIP2 in the membrane

A schematic of the bilayer system depicts two chambers separated by a partition with a hole onto which lipid membranes are painted (Montal and Mueller, 1972; Miller and Racker, 1976; Figure 1B). Components are added by fusing lipid vesicles that contain channels and membrane-anchored G proteins and by adding Na+ or soluble short chain PIP2 molecules to either chamber. The chambers are connected electrically through a voltage-clamp circuit to record K+ currents across the membrane.

Structural depiction of GIRK2 regulation and horizontal planar bilayer configuration.

(A) View from the membrane plane of a GIRK2 channel (gray) bound to its activating ligands PIP2 (purple), Na+ (brown) and the heterodimeric G protein subunit Gβγ (blue, with the postulated geranylgeranyl group on the Gγ subunit drawn as blue lines) in surface representation (PDBID: 4KFM). There are four binding sites for each of these ligands on each GIRK2 homotetramer. (B) A schematic of the horizontal planar bilayer system used to characterize GIRK2 channel activity. Two solution-filled chambers in a polyoxymethylene block are separated by a piece of transparency film with a small hole (∼100 μm diameter). Lipid bilayers are formed across the hole by spontaneous thinning of a painted solution of lipid in decane. Membrane proteins are incorporated into the lipid bilayer by fusion of applied proteoliposomes. Soluble reagents are either directly applied to either chamber followed by thorough mixing or added via a local perfusion system. The two chambers are electrically voltage-clamped as indicated for current recordings.


The current trace in Figure 2A shows K+ currents in bilayer membranes in which GIRK2 channel-containing vesicles were fused in the absence of PIP2 and Gβγ. Brief channel openings appear as downward current spikes because the membrane voltage was held positive relative to ground (top chamber, Figure 1B) and the sign of current was inverted to conform to eletrophysiological convention. When PIP2 was added to the top chamber half way through the trace, channel activity remained essentially unchanged (Figure 2B). Similarly, addition of Gβγ in the absence of PIP2 did not perceptibly alter baseline channel activity (Figure 2C). Only when both PIP2 and Gβγ were present, independent of the order in which they were added, did we observe large K+ currents due to robust GIRK2 channel opening (Figure 2D,E). We note that the baseline current prior to the addition of the second ligand in Figure 2D,E, shown on an expanded current axis scale, is similar in magnitude to the currents in Figure 2B,C. It is thus clear in the bilayer assay that PIP2 or Gβγ alone are insufficient to elicit robust channel activation. Both of these ligands together are necessary. While past studies had reached the conclusion that both ligands are important—that one ligand enhances the affinity of the other (Huang et al., 1998; Yamada et al., 1998; Logothetis and Zhang, 1999)—the complexity of cell membranes obscured the simple conclusion so obvious here that both ligands are necessary to open the channel.

GIRK2 activation requires both Gβγ and PIP2.

Currents are plotted according to electrophysiology convention such that negative values represent inward current with respect to channel orientation. The same buffer containing 150 mM KCl was used in both chambers and the membranes were held at −50 mV (eliciting inward channel current). Initial compositions of each bilayer are indicated above each panel. Fusion of lipid vesicles was done manually and induced significant noise during application. This noise did not interfere with subsequent recording of current change. (A) Current spikes were observed after fusing GIRK2 vesicles into the lipid bilayer. (B and D) Gβγ is necessary for PIP2 activation of GIRK2. Currents were recorded from a bilayer containing either GIRK2 alone (B) or GIRK2+Gβγ (D) before and after application of 32 μM C8-PIP2 (indicated with arrow). Only when Gβγ was present (D) did application of PIP2 increase current through GIRK2. (C and E) PIP2 is necessary for Gβγ activation of GIRK2. Currents were recorded from a bilayer containing either GIRK2 alone (C) or GIRK2 and 2% brain PIP2 (E) before and after application of Gβγ vesicles (indicated with arrow). The amplifier gain was adjusted during the gap in the recording in (E). Insets in (D and E) show spontaneous openings of GIRK2 before application of Gβγ (compare to B and C). (F) Current recorded from a bilayer with GIRK2 and PIP2 before and after application of non-lipidated Gβγ (left side of recording) followed by application of lipidated Gβγ vesicles (right side of recording). Lipidated Gβγ (∼15 μM) robustly activated GIRK2 while high concentrations (∼116 μM) of non-lipidated Gβγ only slightly increased channel activity.


Figure 2F addresses the importance of the lipid anchor on the Gβγ subunit. The recording on the left side shows that when mutant Gβγ subunits devoid of the Gγ-linked geranylgeranyl lipid moiety (C68S) were added to solution (to ∼116 μM concentration) channels did not open robustly. When Gβγ subunits containing the lipid moiety (∼15 μM in lipid vesicles) were subsequently applied to the same membrane, large currents were elicited (Figure 2F, right side recording). This experiment shows that the lipid anchor is essential. The likely explanation is, by mediating membrane partitioning, the lipid anchor is required to achieve sufficiently high enough concentrations of Gβγ on the membrane surface to activate GIRK2.

Cellular electrophysiologists have referred to G protein activation of GIRK channels as ‘membrane delimited’ (Soejima and Noma, 1984; Breitwieser and Szabo, 1985; Pfaffinger et al., 1985; Logothetis et al., 1987; Reuveny et al., 1994; Wickman et al., 1994), meaning Gβγ stays stuck to the membrane when it is released from a GPCR and diffuses to its target. Here, this property of membrane targeting mediated by a lipid anchor is nicely replicated in the bilayer assay. The importance of the lipid anchor is also consistent with the orientation of Gβγ in the crystal structure, which shows the geranylgeranylated C-terminus of Gγ pointed toward the membrane (Figure 1A; Whorton and MacKinnon, 2013).

The sidedness of active channels in planar bilayers

In contrast to the membranes of cells, in which channels are inserted with one orientation, in the planar bilayer system channels and Gβγ subunits insert randomly and thus are oriented in both directions. The experiments in Figure 3 show, despite the dual orientation of proteins in the bilayer membrane, that short chain C8-PIP2 activates only one orientation of GIRK2 channels—corresponding to those channels with their physiologically intracellular surface pointed toward the chamber to which PIP2 was added. Demonstration of this fact made use of the GIRK channel inhibitor tertiapin-Q (TPNQ), a derivative of the peptide toxin tertiapin isolated from honeybee venom (Jin and Lu, 1999). TPNQ inhibits GIRK channels by binding to a specific receptor site on the extracellular surface of the channel. In the first experiment GIRK2 channels and Gβγ subunits were reconstituted into bilayer membranes randomly, C8-PIP2 was added to one side (bottom chamber), and an I-V curve was recorded (Figure 3A, squares). The solutions on both sides of the membrane contained equal concentrations of K+ and thus the I-V curve reverses at zero mV, but still exhibits the characteristic inward rectification of the GIRK channel. Next, TPNQ was added to the opposite side (top chamber) and essentially all of the currents were inhibited (Figure 3A, circles). The second experiment is similar to the first except C8-PIP2 and then TPNQ were added to the same side of the membrane (top chamber) (Figure 3C). In this case TPNQ did not block the K+ currents.

Activation of GIRK2 by C8-PIP2 is sided.

(A) Representative current–voltage relationship recorded from a bilayer containing GIRK2 and Gβγ. Robust current was recorded upon addition of C8-PIP2 to the bottom side of the bilayer (black squares) that is blocked by application of TPNQ to the top side (white circles). (B) Schematic of molecular interactions that explain the data in (A). (C and D) Analagous to (A and B), except that C8-PIP2 and TPNQ were applied to the same side of the bilayer. GIRK2 channels that are activated by C8-PIP2 ‘cytoplasmically’ can only be blocked by ‘extracellular’ TPNQ.


The interpretation of each experiment is shown pictorially (Figure 3B,D). C8-PIP2 (and Gβγ) activate a uniformly oriented population of channels from their intracellular surface, whereas oppositely oriented channels are not activated because they are not exposed to PIP2 on their physiologically intracellular side. This conclusion neatly accounts for complete inhibition of currents when TPNQ is added to the side opposite PIP2 (Figure 3A,B) and no inhibition when it is added to the same side (Figure 3C,D).

The role of Gαi1 in GIRK2 channel regulation

The Gα and Gβγ subunits act on specific target proteins to regulate their function. With GDP bound to the catalytic site of Gα, Gα binds to Gβγ, rendering both (Gα and Gβγ) unavailable to their targets. The role of G protein coupled receptors (GPCRs) is, upon stimulus, to catalyze GDP/GTP exchange on Gα and release of both Gα(GTP) and Gβγ to action. After a period of time, being a slow GTPase, Gα catalyzes the conversion of GTP back to GDP, allowing Gα(GDP) to again form a tight complex with Gβγ, thus terminating action (Gilman, 1987).

Gβγ is known to activate GIRK, but as stated in the introduction, several lines of evidence indicate that Gα can also interact directly with the GIRK channel (Huang et al., 1995; Rebois et al., 2006; Rubinstein et al., 2007; Schreibmayer, 2009; Berlin et al., 2010, 2011). The bilayer assay, in which every component is defined, allows a simple approach to address whether Gα plays a direct role in GIRK channel gating. When Gαi1 containing the non-hydrolyzable GTP analog GTPγS is applied to membranes containing GIRK channels there is no activation (Figure 4A). In this experiment GIRK2 channels were pre-fused with bilayer membranes containing 1% brain PIP2 (Balla, 2013). The characteristic brief baseline channel openings are observed, demonstrating that channels are present in the membrane, but no change in current is observed upon subsequent fusion of vesicles containing Gαi1(GTPγS). To confirm the presence of abundant channels, Gβγ-containing vesicles were subsequently fused with the same bilayer membrane and large K+ currents were evoked (Figure 4A, right side of trace). From this experiment it is clear that GIRK2 channels are activated by Gβγ but not by Gαi1(GTPγS), as summarized pictorially (Figure 4B).

i1(GTPγS) does not activate GIRK2.

Recordings were performed as in Figure 2. The increased signal during application of vesicles resulted from opening the chamber for electromagnetic shielding. (A) Current recorded from a bilayer with GIRK2 and 1% brain PIP2 before and after application of Gαi1(GTPγS) (left) followed by application of Gβγ (right). (B) Schematic of molecular interactions that explain the data in (A). Activation by Gβγ but not Gαi1(GTPγS) confirmed the presence of GIRK2 channels in the bilayer and their insensitivity to applied Gαi1.


While Gαi1(GTPγS) fails to affect GIRK2 channel gating in the absence of Gβγ, there remains the possibility that Gαi1(GTPγS) can function together with Gβγ to influence gating. To test this possibility we first fused GIRK2 channels and Gβγ into the bilayer and supplemented the top chamber with 32 μM C8-PIP2, followed by fusion of vesicles containing Gαi1(GTPγS). C8-PIP2 was used instead of brain PIP2 to ensure that all the activated channels would be oriented with their cytoplasmic side facing the top chamber. To facilitate fusion (Fisher and Parker, 1984), vesicles were applied in a solution containing 750 mM KCl. This concentration of KCl near the membrane surface produced a transient reduction of K+ current–until diffusion or mixing restored the local ion composition to bulk values–as shown in the empty vesicle control on the left side of the trace (Figure 5A). Fusion of Gαi1(GTPγS)-containing vesicles appeared essentially identical to empty vesicles (Figure 5B). Thus, GIRK2 channels are unresponsive to Gαi1(GTPγS) even after they have been activated by PIP2 and Gβγ.

i1(GDP) but not Gα i1(GTPγS) deactivates GIRK2 by sequestering Gβγ.

Recordings were performed as in Figure 2. (A and B) Current recording from a bilayer with GIRK2 and Gβγ activated by 32 μM C8-PIP2 before and after (A) addition of empty vesicles followed by (B) addition of Gαi1(GTPγS) vesicles (vesicle additions indicated by arrows). Note that the high salt concentration of the vesicle solution (750 mM KCl) used to facilitate fusion to the bilayer caused a similar transient reduction in K+ current in both (A) and (B). In both cases, current level returned to near pre-vesicle application levels after thorough mixing. (C) Current recording from a bilayer with GIRK2 and Gβγ activated by C8-PIP2 before and after application of vesicles containing Gαi1(GDP) (indicated by arrow). (D) Comparison of the effects of non-lipidated (soluble) Gαi1(GDP) and Gαi1(GTPγS) on activated GIRK2. Current at −50 mV holding potential (normalized to the value before addition of Gαi1) recorded from bilayers (±SEM, n = 3 bilayers) with GIRK2 and Gβγ activated by C8-PIP2 vs concentration of added Gαi1 species is plotted. The lines connecting data points have no theoretical meaning. (E) Illustration of molecular interactions that explain data in (AD).


In contrast, fusion of vesicles containing Gαi1(GDP) caused rapid disappearance of activated GIRK2 currents (Figure 5C). We also studied the effect of Gαi1(GDP) and Gαi1(GTPγS) using soluble Gαi1 subunits (produced in Escherichia coli) that did not contain a covalent membrane lipid anchor. At micromolar concentrations, soluble Gαi1(GDP) closed GIRK2 channels, whereas soluble Gαi1(GTPγS) had only a small effect most likely due to small amounts of Gαi1(GDP) present in the Gαi1(GTPγS) preparation (Figure 5D). Apparently Gαi1(GDP) in solution binds to membrane anchored Gβγ, rendering it unavailable to activate GIRK2, as depicted (Figure 5E). It is interesting to contrast the ability of soluble Gαi1(GDP) to close GIRK2 channels (Figure 5D) with the inability of soluble (lipid anchor-removed) Gβγ to open GIRK2 channels (Figure 2F). This observation suggests differences in the relative affinities of Gβγ for GIRK2 vs Gαi1(GDP) (The equilibrium dissociation constant for Gαi1(GDP) binding to Gβγ is ∼3 nM in Lubrol and ∼10 nM in lipids [Sarvazyan et al., 1998]). These differential affinities are likely important for G protein regulation of channel activity.

PIP2 mole fraction dependence of GIRK2 channel activation

The importance of the signaling lipid PIP2 to GIRK channel gating has been well documented through studies in cells (Huang et al., 1998; Sui et al., 1998; Rohacs et al., 2002; Gamper and Rohacs, 2012). Previous studies augmented PIP2 levels in cell membranes through the addition of PIP2 to undetermined baseline levels or reduced PIP2 levels through the use of PIP2-sequestering antibodies (Huang et al., 1998; Logothetis and Zhang, 1999; Rohacs et al., 2002; Inanobe et al., 2010). These approaches yielded qualitative data on the relationship between GIRK channel activity and PIP2 concentration. Here we aim to describe the quantitative relationship between GIRK channel activity and PIP2 levels.

The bilayer allows quantitative control of lipid composition. In a first experiment GIRK2 channels and Gβγ were reconstituted into planar lipid membranes without PIP2. Soluble C8-PIP2 was then perfused with a microperfusion pipette aimed directly at the membrane to activate channels: during perfusion of C8-PIP2 channels opened and during perfusion of buffer they closed as soluble C8-PIP2 diffused away from the membrane (Figure 6A). Repeating this experiment with different C8-PIP2 concentrations, or applying different concentrations of C8-PIP2 to the bath solution, gave the titration data shown (Figure 6B). In the graph, in order to compare current levels from different membranes, which in general contain different channel numbers, data were normalized to the current value measured in the presence of 28 μM C8-PIP2. The activation curve follows a sigmoid shaped concentration dependence, consistent with activation by multiple PIP2 molecules. The atomic structure shows four binding sites per channel (Figure 1A). The solid and dashed curves correspond to two different models of channel activation: the solid curve to the Hill equation, which assumes cooperativity between sites, and the dashed curve to a model in which all four binding sites must be occupied but binding is independent (non-cooperative). Both models conform to the data reasonably well. The most important point is that the shape of the titration curve is consistent with multiple binding events being required for channel activation.

Concentration dependence of GIRK2 activation by C8- and brain-PIP2.

(A) Current recorded from a bilayer containing GIRK2 and Gβγ during local perfusion of C8-PIP2 (black bars above recording) or buffer (white bars above recording). (B) Plot of current recorded from bilayers with GIRK2 and Gβγ (normalized to current at 28 μM C8-PIP2, mean ± SEM, n = 3 bilayers) vs concentration of C8-PIP2. Hill fit (solid line) gives an apparent dissociation constant of ∼15 μM and a Hill coefficient of ∼3.1. Fit to a non-cooperative model (‘Materials and methods’) in which simultaneous binding of 4 PIP2 molecules to one channel is required for channel opening is also shown (dotted line). (C) Current recorded from a bilayer with GIRK2, Gβγ and 1.38% (mole fraction) brain PIP2 during local perfusion of 32 μM C8-PIP2 (black bars above recording) or buffer (white bars above recording). Channels oriented with their extracellular side facing the PIP2 perfusion chamber were blocked with 100 nM TPNQ in perfusion buffers. As a way to normalize channel numbers in different membranes, the current value during perfusion of buffer was normalized to the current level during perfusion of 32 μM C8-PIP2. (D) Plot of current recorded from bilayers with GIRK2 and Gβγ (mean ± SEM, n = 3 bilayers) vs concentration of brain PIP2 in the membrane. Regression to Hill equation (solid line) resulted in an apparent dissociation constant of ∼0.8% mole fraction brain PIP2 and a Hill coefficient of ∼2.4. The dashed line shows regression to the same non-cooperative four sites model as in (B).


C8-PIP2 is a soluble form of PIP2 that partitions into the membrane. Given that the (unknown) partition coefficient is likely independent of C8-PIP2 concentration, the titration in Figure 6B is valid for assessing the shape of the titration curve. But what we really want to know is the activity dependence of GIRK2 as a function of the mole fraction of PIP2 inside the membrane. The practical difficulty in determining this is each measurement for a given mole fraction has to come from a different membrane; and each membrane has a different number of channels. In other words, to compare membranes we have to normalize with respect to the number of channels. To do this we produced membranes with a desired mole fraction of full-length PIP2 in the lipid mixture, recorded GIRK2 currents, and then applied C8-PIP2 to maximally activate (or nearly so), as shown (Figure 6C). Data from different bilayers could then simply be normalized with respect to their maximally activated current. The graph shows GIRK2 channel activation as a function of PIP2 mole fraction (Figure 6D). Here, as in the case of soluble C8-PIP2, activation is a sigmoid shaped function of PIP2 mole fraction, consistent with a requirement of multiple PIP2 molecules in channel activation. Moreover, the steepest portion of the graph (corresponding to a few tenths to one percent PIP2) is in agreement the known concentration range of PIP2 in the inner leaflet of cell membranes (Heck et al., 2007; van Meer et al., 2008; Balla, 2013). This mole fraction dependence suggests that naturally occurring variations of PIP2 in cell membranes should influence GIRK2 activity level.

Na+ concentration dependence of GIRK2 channel activation

Intracellular Na+ was shown previously to be an activator of GIRK2 channels (Petit-Jacques et al., 1999; Ho and Murrell-Lagnado, 1999a, 1999b; Inanobe et al., 2013). Here we aim to replicate Na+ dependence in the bilayer through quantitative measurements and to further our understanding of Na+ activation by investigating the relationship between Na+ and PIP2 activation. Figure 7 graphs GIRK2 channel activity as a function of internal Na+ or C8-PIP2 concentration (Figure 7A–C). In these experiments GIRK2 channels and twofold excess Gβγ (per channel subunit) were reconstituted into bilayer membranes. The 3 dimensional plot shows how these ligands' effects are interdependent (Figure 7A). As shown earlier, robust channel opening is absolutely dependent on the presence of both PIP2 and Gβγ (Figure 7C). By contrast, GIRK2 channels open in the absence of internal Na+, but opening increases as the Na+ concentration is increased. Viewed along the axis of PIP2 activation, the presence of internal Na+ steepens the dependence of channel opening on PIP2 concentration (Figure 7B). Thus, Na+ is not essential but augments channel opening. The effect of Na+ depends on the degree of activation by PIP2. In the presence of ample PIP2, channel activity increases as a function of Na+ concentration most steeply in the range 0–10 mM. This dependence seems well matched to the concentration range over which cytoplasmic Na+ levels change in response to excessive electrical excitation (Muller and Somjen, 2000; Somjen, 2002).

Interdependence of PIP2 and Na+ activation.

Channel activity is normalized to that at the condition in which 28 μM of PIP2 and 32 mM of Na+ is present. (A) A 3-D representation of the activation landscape of PIP2 and Na+. The surface is gradient colored according to activation. (B) Plot of percentage activity of GIRK2 channels in membranes with saturating Gβγ vs added C8-PIP2 concentration in the absence (red, n = 3) and presence (black, n = 3) of 32 mM Na+. PIP2 activated GIRK2 currents were ∼2.5-fold higher (at 28 μM) in the presence of 32 mM Na+. Hill fitting resulted in apparent dissociation constants of ∼15 μM and ∼12 μM and Hill coefficients of ∼3.1 and ∼1.5 in the absence and presence of Na+, respectively. (C) Plot of percentage activity of GIRK2 channels in membranes with saturating Gβγ vs concentration of added Na+ in the absence (red, n = 3) and presence (black, n = 3) of 28 μM C8-PIP2. The titration data were fit to a simple 1:1 dose response model with an apparent dissociation constant of ∼9 mM.



One question addressed in this study, motivated by past work showing that Gα can interact physically with at least some GIRK channels (Yatani et al., 1988; Huang et al., 1995; Peleg et al., 2002; Ivanina et al., 2004; Berlin et al., 2011), concerns the role of Gα vs Gβγ in GIRK channel activation. Using bilayer reconstitution we find the following. Gβγ activates GIRK2, as was previously shown (Logothetis et al., 1987; Reuveny et al., 1994; Wickman et al., 1994; Kofuji et al., 1995). Gαi1(GTP) does not activate GIRK2 and moreover does not appear to influence the activation of GIRK2 by Gβγ. These findings suggest, at least for the case of the activated form of Gαi1 (Gαi1(GTP)) and GIRK2, that physical interactions between these proteins, if they indeed exist, are without direct functional consequence.

i1 inhibits the GIRK2 channel activity only when it is in its GDP bound form, consistent with the canonical view that GIRK is activated by Gβγ and that GDP-bound Gαi1 sequesters Gβγ (Gilman, 1987; Wickman et al., 1994; Peleg et al., 2002). In the bilayer experiments the action of Gαi1(GDP) is so rapid (Figure 5C) as to suggest that it might bind to the channel and cause Gβγ to dissociate. We think, however, that such a displacement mechanism need not be invoked to account for the rapid inhibitory effect of Gαi1(GDP). In drawing this conclusion we appeal to the inefficiency of Gβγ to activate when it does not contain a lipid anchor. Presumably Gβγ fails under this circumstance because, without membrane partitioning, its local concentration near the channel is never high enough to achieve sufficient occupancy to open the channel. Thus, Gβγ apparently binds to the channel with low affinity, compatible with a rapid dissociation rate. Comparatively, Gβγ appears to bind Gαi1(GDP) with high affinity, as this would account for sequestration of Gβγ even when no lipid is present on Gαi1(GDP) (Figure 5D). Therefore, it seems likely that Gαi1(GDP) turns off GIRK by simply binding to the free (channel unbound) form of Gβγ and shifting the equilibrium away from channel occupancy by Gβγ.

Several further lines of evidence support the notion that Gβγ binds with low affinity to the GIRK channel. During biochemical purification we have never detected a complex between these two proteins on gel filtration even when both proteins are at concentrations in the 100 µM range (Whorton and MacKinnon, 2013). Using solution NMR spectroscopy, Shimada et al. estimated the affinity between Gβγ and the GIRK1 cytoplasmic domain (which accounts for the full protein–protein interaction surface) to be about 250 μM (Yokogawa et al., 2011). Using this value, if we conservatively assume an association rate of 105 M−1s−1 (well below the expected diffusion limit in solution) then the dwell time for a single Gβγ on the channel is calculated to be about 100 ms, a duration much shorter than the wash out time in our experiments. Finally, a low affinity interaction matches the relatively small surface area (∼700 Å2) of interaction between Gβγ and its binding site on GIRK2 in the crystal structure (Whorton and MacKinnon, 2013). Therefore, all observations are consistent with the idea that Gβγ is in rapid exchange with its binding site on the GIRK channel.

Given the apparent low affinity between Gβγ and the GIRK channel, the high affinity of Gβγ for Gα(GDP), and the absence of an effect of Gα on channel function, we think the following mechanism is most likely. GPCR activation generates Gβγ and Gα(GTP), then Gβγ activates GIRK, but once GTP is hydrolyzed the resultant Gα(GDP) removes Gβγ from the channel by equilibrium-mass action. This picture is simple and in good agreement with the accepted view, but it raises the following question. If the affinity of Gβγ for GIRK is so low and the interaction so transient, how does GPCR activation ever achieve sufficiently high concentrations of Gβγ in the membrane to activate the channel? It seems unlikely that a single GPCR located near a GIRK channel would be able to generate a high enough local concentration of subunits to activate it, especially if multiple bound Gβγ subunits are required, which we suspect is the case on the basis of the crystal structure (Whorton and MacKinnon, 2013). One way to explain this dilemma is to imagine that GIRK channels and GPCRs might be co-localized in relatively large patches on the membrane. Such an organization would exploit a fundamental property of 2-dimensional diffusion: a point source in an infinite space has no steady state solution. In other words, the transition between local and bulk Gβγ concentration will grow radially with activation time. Thus, in a patch of multiple GPCRs (and channels) a sufficiently high concentration of Gβγ on the membrane could be reached. The possibility of co-localization of GPCRs and GIRK channels raises an interesting challenge to the replication of cell-like signaling in the bilayer. It could be that additional cellular elements (e.g., cytoskeleton) are required to perfectly replicate the signaling behavior observed in cells.

Another question addressed in this study concerns the quantitative dependence of GIRK2 channel activity on the concentration of PIP2 in the membrane. In the presence of Gβγ, GIRK2 channel activity shows a sigmoid dependence on PIP2 mole fraction. A Hill plot yields a coefficient around 2.5. We cannot tell from our data whether PIP2 binding is cooperative (as the Hill model assumes) or non-cooperative (e.g., if multiple PIP2 molecules must bind, but independently, to open the channel). Cooperative or not, the sigmoid functional relationship points to multiple PIP2 molecules required to open the channel. This result is consistent with the crystal structures, which show four PIP2 molecules bound to the channel (Whorton and MacKinnon, 2011). The apparent binding constant for PIP2 is 0.8% mole fraction (Hill model), which, together with the Hill coefficient, describes the position of the steepest part of the sigmoid curve along the mole fraction axis. We see that GIRK2 activity is most responsive to PIP2 in the mole fraction range 0.1–1.0%. This is precisely the range over which PIP2 is known to vary in the inner membrane leaflet of cells (Balla, 2013). In other words, GIRK2 is poised to be under control by PIP2 membrane concentration.

Finally this study addressed the interdependence of multiple ligands known to activate GIRK channels. Robust channel opening requires the presence of both Gβγ and PIP2. Earlier electrophysiological studies concluded that activation can occur at high concentrations of a single ligand and that the role of Gβγ, for example, was to enhance the stimulation brought about by PIP2 (Huang et al., 1998; Sui et al., 1998). Those studies were carried out in cells, where the composition of the membrane is not so certain. The planar bilayer experiments support a simple conclusion: Gβγ and PIP2 are simultaneously required to activate GIRK2. This conclusion is beautifully compatible with structural studies of GIRK2, which show that when PIP2 alone is bound the channel's inner gate remains closed (it exhibits the same closed conformation as when PIP2 is not bound) (Whorton and MacKinnon, 2011). But when both Gβγ and PIP2 are bound the cytoplasmic domain rotates with respect to the transmembrane domain and causes the inner helix gate to begin opening (Whorton and MacKinnon, 2013). PIP2 binds near the interface between the cytoplasmic domain and the transmembrane domain. We hypothesize that PIP2 strengthens allosteric communication between these two domains of the channel. Such a strengthening of interaction would allow conformational changes in the cytoplasmic domain, induced by Gβγ binding, to open the gate in the transmembrane domain.

Na+ is not sufficient and not necessary for GIRK2 channel activation. Na+ increases channel opening in the presence of Gβγ and PIP2 and thus Na+ is best described as a modulator of the other ligands' effects (Figure 7C). This conclusion is easily appreciated through inspection of the C8-PIP2 activation curve in the presence and absence of Na+ (Figure 7B). Not only does Na+ increase the current level at any given concentration of PIP2 (and a fixed concentration of Gβγ), but the shape of the PIP2 dependence curve is affected by Na+. In other words, Na+ does not simply scale the PIP2 dependence curve. This is explicable if the binding affinity/effect of PIP2 is coupled to the presence of Na+ (Ho and Murrell-Lagnado, 1999a, 1999b). In the crystal structure Na+ binds at a location in between Gβγ and PIP2. It is therefore reasonable to think that conformational changes induced by the binding of one ligand, for example Na+, would have consequences for the binding/effect of another ligand, for example Gβγ or PIP2. Although Na+ plays a modulatory rather than obligatory role in gating, its effect is substantial and occurs over the range in which cytoplasmic Na+ concentration is known to change when a cell is experiencing excessive electrical excitation (Muller and Somjen, 2000). These findings are in good agreement with the hypothesis that Na+ modulation renders GIRK2 a negative feedback safety element (Ho and Murrell-Lagnado, 1999a).

The dual requirement for both Gβγ and PIP2 may have important consequences for the control of cellular electrical excitability by different signaling pathways. Consider that Gβγ is released upon stimulation of all GPCRs, but GIRK channels only open specifically in response to Gi/o coupled GPCRs (Gi/o refers to G protein trimers containing i/o class Gα subunits) (Breitwieser and Szabo, 1985; Pfaffinger et al., 1985). It is easy to understand a possible origin of specificity when comparing Gi/o coupled GPCRs (which activate GIRK) and Gq coupled GPCRs (which do not activate GIRK) (Lei et al., 2001; Cho et al., 2005). Gq coupled GPCRs release Gβγ, but at the same time the Gαq subunit activates PLC, which depletes PIP2 through hydrolysis (Cho et al., 2005; Keselman et al., 2007; Sohn et al., 2007). In the absence of sufficient PIP2, Gβγ cannot activate GIRK. Indeed, recent experiments have shown in both atrial pacemaker cells (Cho et al., 2005) and certain neurons (Sohn et al., 2007; Yamamoto et al., 2014) that Gq coupled GPCR stimulation inhibits GIRK activation by Gi/o coupled GPCR stimulation. It is thus reasonable to think that absence of PIP2 accounts at least in part for the inability of Gq coupled GPCRs to activate GIRK. Gi/o coupled GPCRs on the other hand generate Gβγ without degrading PIP2 so both necessary ligands are available to activate GIRK. This kind of GPCR selectivity results because GIRK is an AND gate with respect to Gβγ and PIP2 activation. In addition to the more widespread (but not yet proved) idea of GPCR/target protein co-localization (Fowler et al., 2007; Labouebe et al., 2007; Cui et al., 2010; Luscher and Slesinger, 2010), AND gating of GPCR targets by multiple ligands provides a mechanism for selectivity in G protein signaling.

Materials and methods

Protein expression and purification

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Mouse GIRK2 (amino acid residues 52–380) was expressed in Pichia pastoris cells, extracted with decyl-β-D-maltopyranoside (DM) and purified as previously described (Whorton and MacKinnon, 2011). The protein was concentrated to ∼15 mg/ml after purification and used immediately for reconstitution.

Human G protein subunits β1 and γ2 were expressed in High Five (Invitrogen, Carlsbad, CA) insect cells by co-infection as previously described (Whorton and MacKinnon, 2013). Cell membranes were then prepared and G proteins were extracted from the membranes with Na-cholate (Sigma, St. Louis, MO) and purified as described (Whorton and MacKinnon, 2013) with the following modifications: 1% (wt/vol) of Na-Cholate was used in Talon resin (Clontech, Mountain View, CA) purification instead of 0.5% (wt/vol) of Anzergent 3–12 (Anatrace, Maumee, OH). After elution from Talon resin and digestion with PreScission protease, the protein was diluted to an imidazole concentration of 10 mM. The diluted solution was passed through a column packed with Talon resin pre-equilibrated with the same buffer without imidazole. The flow-through from this step was concentrated and loaded onto a Superdex 200 10/300 (GE Healthcare, Pittsburgh, PA) gel filtration column in the buffer 20 mM HEPES pH 8.0, 150 mM KCl, 5 mM DTT, 1 mM EDTA, 0.2% DM. The hetero-dimer Gβγ protein eluted as a major peak at ∼12 ml. Fractions containing this peak were pooled and concentrated to ∼30 mg/ml and were either used immediately or frozen in aliquots at −80°C.

The non-lipidated form of Gβγ was expressed by co-infection of High Five cells with two baculoviruses each containing Gβ1 and Gγ2 C68S mutant genes. The purification methods are the same as wild-type Gβγ except that no detergent was used.

For the preparation of Gαi1 protein, cDNA of human Gαi1 was cloned into pFastBac (Invitrogen) vector without any affinity purification tag. High Five insect cells were co-infected with three baculoviruses each bearing one of the following proteins: Gαi1, Gβ1 and Gγ2. Gαi1 was purified by first binding the Gαi1βγ heterotrimer to Talon resin and then eluting just the Gαi1 subunit by dissociating it from the heterotrimer with aluminum tetrafluoride AIF4 as previously described (Kozasa, 2004). Briefly, cell membranes containing all three G protein subunits were extracted with Na-cholate in the buffer system used for Gβγ purification supplemented with 3 mM MgCl2 and 10 µM GDP. After binding the extracted protein to Talon resin and washing with 10 mM imidazole, Gαi1 was eluted in the same buffer supplemented with 50 mM MgCl2, 30 µM AlCl3, 10 mM NaF, and 30 µM GDP. Since this elution step specifically disrupts the Gαi1-Gβγ interaction, the eluted fraction is fairly pure. The eluent is then concentrated and loaded onto a Superdex 200 10/300 gel filtration column in the buffer 20 mM HEPES pH 8.0, 150 mM KCl, 5 mM DTT, 2 mM MgCl2, 0.2% DM. Gαi1 eluted as a major peak at ∼14.5 ml. Fractions containing this peak were pooled and concentrated to ∼10 mg/ml. For exchange of nucleotide on Gαi1, either 2 mM GDP or GTPγS were added to the protein, then incubated at 37°C for 30 min. Slight precipitation usually occurs, but can be clarified by centrifugation at 4°C. The binding status of Gαi1 was assayed by a controlled trypsin digestion assay (Mazzoni et al., 1991; Marin et al., 2001) in which the activated form of Gαi1 (GTPγS) is protected from digestion by N-tosyl-L-phenylalanyl chloromethyl ketone (TPCK) treated trypsin. Purified Gαi1 proteins were put on ice until used.

To obtain non-lipidated Gαi1 protein, Gαi1 cDNA was cloned into pET-28a(+) vector (Novagen, Billerica, MA) with an N-terminal hexahistidine tag and transformed into BL21 E. coli for protein expression. In order to obtain non-lipidated protein, we did not co-express with N-myristoyltransferase (NMT) (Mumby and Linder, 1994). At an OD600 of ∼0.9. 1 mM IPTG was used to induce expression, which was continued at 37°C for 8 hr. The same buffers were used for non-lipidated Gαi1 purification as for non-lipidated Gβγ except that MgCl2 was added to 2 mM and GDP to 10 µM. The purification steps are essentially the same as non-lipidated Gβγ. After gel filtration, protein was concentrated and nucleotide exchange (GDP or GTPγS) was performed as described above for lipidated Gαi1. The function of the proteins were tested with a histidine tag based pull down assay using Gβγ.

Reconstitution of proteoliposomes

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A lipid mixture composed of 3:1 (wt:wt) 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) : 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (POPG) was used for reconstitution of GIRK2 channels and G proteins into lipid vesicles. The reconstitution procedure is similar to that previously reported (Heginbotham et al., 1999; Long et al., 2007; Tao and MacKinnon, 2008; Brohawn et al., 2012) with some modifications. Briefly, 20 mg/ml of the above lipid mixture was dispersed by sonication and then solubilized with 20 mM DM. GIRK2 and Gβγ were diluted with reconstitution buffer (10 mM potassium phosphate pH 7.4, 150 mM KCl, 1 mM EDTA and 3 mM DTT) supplemented with 0.2% DM to 2 mg/ml (∼51 µM) and 4.8 mg/ml (∼106 µM), respectively. Equal volumes of 20 mg/ml solubilized lipid mixture was combined with each of these protein solutions to make protein: lipid (wt:wt) ratios of 1:10 and 1:4.2 with a final lipid concentration of 10 mg/ml. For co-reconstitution of GIRK2 with Gβγ, the two proteins were mixed, resulting in final concentrations of 2 mg/ml and 4.8 mg/ml respectively. Subsequently, this solution was mixed with an equal volume of 20 mg/ml solubilized lipid mixture. Detergent was removed by dialysis against reconstitution buffer at 4°C for 4–6 days. For reconstitution of Gαi1(GTPγS) and Gαi1(GDP), the proteins were diluted to 4 mg/ml (∼100 µM) with reconstitution buffer supplemented with 0.2% DM and 2 mM of respective nucleotides. After mixing the diluted Gαi1 protein solutions with equal volumes of 20 mg/ml solubilized lipid mixture, the detergent was removed by dialysis against reconstitution buffer supplemented with either 10 μM GTPγS or GDP, at 4°C for 4–6 days. The resulting proteoliposomes were flash-frozen with liquid nitrogen in 20 μl aliquots and stored at −80°C until needed.


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Bilayer experiments were performed as previously described (Ruta et al., 2003) with the following modifications: 20 mg/ml of a lipid solution in decane composed of 2:1:1 (wt:wt:wt) of 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) : 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) : 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (POPS) was painted over a ∼100 μm hole on a piece of polyethylene terephthalate transparency film that separates two chambers in a polyoxymethylene block (Figure 1B). In some experiments, 1–4% (wt%) of brain PIP2 (Avanti Polar Lipids, Alabaster, AL) was added into the lipid mixture. In most experiments the same buffer (10 mM potassium phosphate pH 7.4, 150 mM KCl, 1 mM EDTA) was used in both chambers. Voltage across the lipid bilayer was clamped with an Axopatch 200B amplifier in whole-cell mode. The analog current signal was low-pass filtered at 1 kHz (Bessel) and digitized at 20 kHz with a Digidata 1322A digitizer. Digitized data were recorded on a computer using the software pClamp (Molecular Devices, Sunnyvale, CA). For local perfusion, a SmartSquirt8 ValveLink Micro-Perfusion System (Automate Scientific, Berkeley, CA) was used with a 250 μm diameter perfusion pencil positioned just above the lipid bilayer. Recordings were performed at room temperature.

The Hill equation used for fitting titration data is:

(1) I=I0+(ImaxI0)xnkn+xn,

where I is the observed current, I0 is the basal current (spontaneous opening current and leak, very close to 0), Imax is the maximum current when channels are fully activated, x is ligand concentration and n is the Hill coefficient.

Under the assumption that binding of each PIP2 to GIRK2 is non-cooperative (independent), the fraction of PIP2 binding sites occupied was expressed as:

(2) q=xkd+x,

where q is the fraction of occupied PIP2 binding sites, x is ligand concentration and kd is the apparent dissociation constant. For a channel with four PIP2 binding sites the fraction of channels with all four sites occupied is:

(3) p=(44)×q4×(1q)0=q4.

If channel opening corresponds (is equivalent) to occupancy by four PIP2 molecules then the total current is:

(4) I=Imax×p,

where Imax is the maximum current when all channels are activated. Substituting Equation 2 and 3 into Equation 4 gives

(5) I=Imax×(xkd+x)4.

We used Equation 5 in fitting titration data points. Although likely overly-simplified, the purpose of using this model is to emphasize that a sigmoidal dose–response does not necessarily indicate cooperativity among binding sites.


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Decision letter

  1. Richard Aldrich
    Reviewing Editor; The University of Texas at Austin, United States

eLife posts the editorial decision letter and author response on a selection of the published articles (subject to the approval of the authors). An edited version of the letter sent to the authors after peer review is shown, indicating the substantive concerns or comments; minor concerns are not usually shown. Reviewers have the opportunity to discuss the decision before the letter is sent (see review process). Similarly, the author response typically shows only responses to the major concerns raised by the reviewers.

Thank you for sending your work entitled “Quantitative analysis of mammalian GIRK2 channel regulation by G proteins, PIP2 and Na+ in a reconstituted system” for consideration at eLife. Your article has been favorably evaluated by John Kuriyan (Senior editor) and Richard Aldrich (Board of Reviewing Editors) and two external reviewers (Donald Hilgemann and Christopher Miller).

The Reviewing editor and the other reviewers discussed their comments before we reached this decision, and the Reviewing editor has assembled the following comments to help you prepare a revised submission.

In this elegant, thorough, and important fundamental analysis of GIRK activation mechanisms, the authors exploit the membrane reconstitution approach for precisely the purposes le bon Dieu (Ef Racker) invented it. For what seems like millennia of time and kilograms of publications on cellular patch-recording, GIRK channels have been understood to be activated on the intracellular side by G-proteins, PIP2, and in some case Na+ ions. But the complexity of the cell membrane, even when detached from the cell itself, has stymied a quantitative analysis of these effectors. This is not a persnickety pedant's problem of being unable to dot some inconsequential i's and cross some trivial t's, but is a basic deficit that has persistently prevented the field from developing a satisfactory picture of GIRK function. For many channels, such functional landscapes were well worked out long before crystal structures appeared, but for GIRK it's the reverse situation. The various GIRK structures that have been emerging from MacKinnon's lab over past years have been a bit thin on the function that the structures would be so valuable in explaining. Instead, features such as PIP2 and Na+ activation had almost to be “read into” the new structures rather than being explained by them.

With this paper, the situation changes. Now, the important GIRK effectors are examined with full reductionist control, of both protein and lipid bilayer composition: G-protein subunits charged with relevant nucleotides, PIP2 etc, all in quantitatively manipulable amounts. The conclusions are not surprising – the basic lessons have been long suspected, both from qualitative probing by cellular electrophysiology and by MacKinnon's previous GIRK structures – but they are deeply satisfying and now quantitative. In essence, the functions explain the structures, for a refreshing change. Particularly important is – at last – a proper activation curve for PIP2, real PIP2 from real brains, along with a comparison to the convenient, commonly used exogenous C8-analog. It's also satisfying to see a Hill-fit (nH=2.7) accompanied by an unapologetic assertion that this, while consistent with involvement of 4 binding sites, long-envisioned in cartoons and now seen in crystal structures, cannot adequately distinguish cooperative from independent activator binding. In addition, the effect of Na+ as a modulator of PIP2 action is clearly shown by direct experiments, simple on paper but never before properly implemented. The authors even treat old-timers with a brief historical frisson by re-visiting the non-role of Gα, lubrol and all, reminding them of the Great Clapham-Brown Debates of yore that established for the first time the central place of Gβγ in GIRK function. Finally, the exposition relates the mechanistic minutiae uncovered here to central biological roles of GIRKs in cardiac and neuronal electrical behaviors.

The main issue that needs to be addressed is that the authors need to more clearly lay out the state of understanding of this system previous to their present contributions. They should more directly describe the context, within the existing knowledge, of each of their experimental conclusions and more greatly emphasize which of their conclusions are confirmatory (but done in a superior experimental system) and which are unprecedented.


Author response

The authors need to more clearly lay out the state of understanding of this system previous to their present contributions. They should more directly describe the context, within the existing knowledge, of each of their experimental conclusions and more greatly emphasize which of their conclusions are confirmatory (but done in a superior experimental system) and which are unprecedented.

To this issue the Introduction is (and was) structured with a second paragraph describing what is known: “As background to the questions addressed in the present study, the following conclusions have been established over the past several decades of study…” This is followed by a third paragraph: “The present study addresses the following still unanswered questions…”

For further clarification we have added to the Results section a statement emphasizing the new understanding of an obligatory dual requirement for both Gbg and PIP2 and its distinction from past descriptions. This is again presented in the Discussion section on the concept of GIRK as an AND gate.

We have also added to the Results section an introductory paragraph under the subheading “PIP2 mole fraction dependence…” describing what was known and what we are aiming to understand. A similar explanatory introduction has been added under the subheading “Na+ concentration dependence…”

The first paragraph of the Discussion (and beginning of the second) has been rewritten to emphasize what was known about Gbg versus Ga interactions with GIRK and why the question of a potential role for Ga is still relevant today. Our experiments show no functional effect, but the case remains unsettled for GIRK1 and Gai3 and other pairings.

We hope these additions are clarifying and not too redundant regards what is confirmatory (i.e., that Gbg activates and Ga does not), what represents a more quantitative understanding (i.e. GIRK2 activation as a function of PIP2 mole fraction and as a function of Na+), and what is new (i.e., the absolute requirement for both Gbg and PIP2 and the relationship between PIP2 and Na+ in GIRK2 activation).


Article and author information

Author details

  1. Weiwei Wang

    Laboratory of Molecular Neurobiology and Biophysics, Howard Hughes Medical Institute, The Rockefeller University, New York, United States
    WW, Conception and design, Acquisition of data, Analysis and interpretation of data, Drafting or revising the article
    Competing interests
    The authors declare that no competing interests exist.
  2. Matthew R Whorton

    Laboratory of Molecular Neurobiology and Biophysics, Howard Hughes Medical Institute, The Rockefeller University, New York, United States
    MRW, Conception and design, Drafting or revising the article
    Competing interests
    The authors declare that no competing interests exist.
  3. Roderick MacKinnon

    Laboratory of Molecular Neurobiology and Biophysics, Howard Hughes Medical Institute, The Rockefeller University, New York, United States
    RMK, Conception and design, Analysis and interpretation of data, Drafting or revising the article
    For correspondence
    Competing interests
    The authors declare that no competing interests exist.


Howard Hughes Medical Institute

  • Roderick MacKinnon

National Institute of General Medical Sciences (NIHGM43949)

  • Roderick MacKinnon

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.


We thank Joel Butterwick, Xiao Tao and Emily Brown for advice on biochemistry and reconstitution of the channels; Stephen Brohawn for comments on the manuscript; Y Hsiung for assistance with insect cell culture; and members of the MacKinnon laboratory for helpful discussions. This work was supported in part by NIHGM43949. RM is an investigator in the Howard Hughes Medical Institute.

Reviewing Editor

  1. Richard Aldrich, The University of Texas at Austin, United States

Publication history

  1. Received: June 13, 2014
  2. Accepted: July 17, 2014
  3. Accepted Manuscript published: July 20, 2014 (version 1)
  4. Version of Record published: August 19, 2014 (version 2)


© 2014, Wang et al.

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.


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  1. Weiwei Wang
  2. Matthew R Whorton
  3. Roderick MacKinnon
Quantitative analysis of mammalian GIRK2 channel regulation by G proteins, the signaling lipid PIP2 and Na+ in a reconstituted system
eLife 3:e03671.

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