Mitochondrial Ca2+ uptake, a process crucial for bioenergetics and Ca2+ signaling, is catalyzed by the mitochondrial calcium uniporter. The uniporter is a multi-subunit Ca2+-activated Ca2+ channel, with the Ca2+ pore formed by the MCU protein and Ca2+-dependent activation mediated by MICU subunits. Recently, a mitochondrial inner membrane protein EMRE was identified as a uniporter subunit absolutely required for Ca2+ permeation. However, the molecular mechanism and regulatory purpose of EMRE remain largely unexplored. Here, we determine the transmembrane orientation of EMRE, and show that its known MCU-activating function is mediated by the interaction of transmembrane helices from both proteins. We also reveal a second function of EMRE: to maintain tight MICU regulation of the MCU pore, a role that requires EMRE to bind MICU1 using its conserved C-terminal polyaspartate tail. This dual functionality of EMRE ensures that all transport-competent uniporters are tightly regulated, responding appropriately to a dynamic intracellular Ca2+ landscape.https://doi.org/10.7554/eLife.15545.001
Like all power plants, mitochondria – the compartments inside our cells that supply energy – must adjust their energy output to match fluctuations in demand. Inside cells, the levels of calcium ions in the cytoplasm often signal such demands. Mitochondria therefore control their calcium ion levels with tightly regulated, membrane-embedded proteins that move calcium ions into and out of the mitochondria. One of these membrane machines, the mitochondrial calcium uniporter (MCU) complex, is a "smart channel" that admits calcium ions into the mitochondria only when their cytoplasmic levels exceed a threshold.
The MCU complex contains four essential proteins: MCU, which forms the pore through which the calcium ions enter the mitochondrion; MICU1 and MICU2, which act as “gatekeepers”, opening the pore only when the cell contains high levels of calcium ions; and EMRE, a small, mysterious protein. Why is EMRE required for the channel's operation, and how does it fit into the four-protein complex?
By comparing EMRE proteins from different species, constructing mutant forms of EMRE, and recording calcium ion transport in mitochondria from cultured human cells, Tsai, Phillips et al. show that EMRE has two key roles. First, it snuggles up against the MCU protein and forms an essential part of the calcium ion-selective pore. Second, it acts as molecular glue to fix the calcium ion-sensing MICU gatekeepers to the pore. These two linked functions ensure that the MCU complex switches on only when the cell contains high levels of calcium ions, preventing the cell becoming catastrophically overloaded with calcium ions and cell death.
Challenges for the future are to purify the MCU complex and reconstitute its ability to transport calcium ions from its component parts. This will help to determine the structure of the channel.https://doi.org/10.7554/eLife.15545.002
Ca2+ regulation of key mitochondrial processes such as ATP production and initiation of apoptosis is controlled by precise balance of Ca2+ influx and efflux across the mitochondrial inner membrane (Gunter et al., 2000; Rizzuto et al., 2012). Studies in the 1960s and '70s established that mitochondria from most eukaryotes, except for certain yeast species, can take up large quantities of Ca2+ from the cytosol into the matrix through a mechanism that is membrane potential dependent and strongly inhibited by ruthenium compounds such as Ru360 (Carafoli and Lehninger, 1971; Deluca and Engstrom, 1961; Ying et al., 1991). A few years ago, the field witnessed a groundbreaking achievement — identification of the MCU gene (Baughman et al., 2011; De Stefani et al., 2011). The 35-kDa MCU protein oliogomerizes with unknown stoichiometry to form a Ca2+-selective pore (Baughman et al., 2011). MCU possesses two transmembrane helices (TMHs) connected by a short loop that hosts a signature sequence (DIME) thought to contribute to a Ca2+-selective permeation site. The N- and C-terminal regions of MCU are exposed to the mitochondrial matrix, each with a coiled-coil sequence of unknown function.
It was subsequently found that MCU forms a complex with the mitochondrial Ca2+ uptake protein 1 (MICU1), which has co-evolved with MCU since early eukaryotic evolution (Baughman et al., 2011; Bick et al., 2012). In humans, MICU1 has two additional homologues, MICU2 and the neuron-specific MICU3 (Plovanich et al., 2013). The MICUs serve as the Ca2+-sensing gate that confers Ca2+-dependence to opening of the Ca2+-selective pore (Csordas et al., 2013; Mallilankaraman et al., 2012). In resting cellular conditions, where cytoplasmic Ca2+ is low, MICUs shut the pore to prevent excessive Ca2+ influx into the matrix, a dangerous process that could diminish inner membrane potential and trigger apoptotic cell death. Transient elevation of Ca2+ to the low μM range, detected by EF-hands in MICUs, releases this inhibition to open the channel (Csordas et al., 2013; Kamer and Mootha, 2014). To avoid confusion on nomenclature, we henceforth refer to the Ru-360 sensitive mitochondrial Ca2+ channel complex as the 'uniporter complex,' a molecular assembly of the pore-forming MCU protein along with associated regulatory subunits.
Recently, using quantitative mass spectroscopy, Mootha and colleagues discovered yet another component of the uniporter complex: the essential MCU regulator (EMRE), a small (~10 kDa) inner membrane protein found only in metazoa (Sancak et al., 2013). EMRE possesses a single TMH and a highly conserved C-terminal polyaspartate tail, typically composed of one glutamate followed by 5–7 aspartates. In humans, MCU-EMRE interaction is absolutely required for Ca2+ permeation via MCU (Kovacs-Bogdan et al., 2014; Sancak et al., 2013). However, an MCU homologue in D. discoideum, a species belonging to the EMRE-lacking Amoebazoa group in protists, is fully capable of conducting Ca2+ (Kovacs-Bogdan et al., 2014). The question naturally arises: what might be the physiological importance for MCU to become strictly dependent on EMRE for function in humans? What would be the consequence if human MCU could transport Ca2+ without EMRE?
We address these questions by mounting an extensive investigation of EMRE. We first sought to determine the protein's transmembrane topology, a problem that cannot be definitively resolved by standard protease digestion assays (Baughman et al., 2011; Vais et al., 2016) due to the small size of the protein’s extra-membrane regions. Two alternative strategies – directed mass-tagging and MCU-EMRE fusion construction - establish that EMRE exposes its N-terminal region to the matrix and C-terminus to the intermembrane space (IMS). Mutagenesis screening and domain-interaction analysis further demonstrate that EMRE supports Ca2+ transport by using its TMH to bind to MCU through its first TMH (TMH1). Moreover, EMRE also interacts with MICU1 via its C-terminal polyaspartate tail, a molecular contact that turns out to be crucial to retain MICUs in the uniporter complex to gate the MCU pore. These results lead to a molecular model wherein the dual 'MCU-activating' and 'MICU-retaining' functionalities of EMRE together play a crucial role in orchestrating uniporter responses to intracellular Ca2+ signaling.
To study uniporter subunits without interference from native mitochondrial proteins, we employed CRISPR/Cas9 to produce MCU-knockout (KO), EMRE-KO, or MCU/EMRE double KO (ME-KO) HEK 293 cell lines. A standard Ca2+ flux assay was used to evaluate uniporter activity. In a typical experiment, HEK cells were permeabilized with digitonin in the presence of a Ca2+-sensing fluorophore (CG-5N) and then treated with 10 μM extracellular Ca2+ (Figure 1A). In WT cells, Ca2+ is rapidly sequestered by mitochondria, and Ru360, a potent MCU inhibitor, arrests the uptake immediately (Figure 1A). (Henceforth for clearer data presentation, only the response of permeabilized cells to Ca2+ will be presented, as in the red box in Figure 1A, with arrowheads indicating Ru360 addition.) Consistent with previous reports (Baughman et al., 2011; De Stefani et al., 2011; Sancak et al., 2013), EMRE-, MCU-, or ME-KO mitochondria are completely devoid of uniporter activity, a deficiency rescued by supplying the deleted genes (Figure 1B).
Genome sequences imply that MCU and MICU proteins are ancient in eukaryotic evolution, while EMRE sequences appear only among metazoa. Indeed, MCU homologues from an amoeba, D. discoideum, and a green plant, A. thaliana, both of which lack EMRE, can alone mediate rapid, Ru360-sensitive mitochondrial Ca2+ uptake in ME-KO cells (Figure 1C). In contrast, metazoan MCU homologues from C. elegans and D. melanogaster require EMRE to transport Ca2+ (Figure 1C), as in humans. This striking difference raises questions regarding the biological purpose of EMRE in animals and offers opportunities for attacking questions of molecular mechanism.
To approach the physiological importance of EMRE’s regulatory function, we first ask how it assembles with other uniporter subunits into a channel complex. The orientation of EMRE in the inner membrane was determined by a cysteine-modification, mass-tagging method. In a typical experiment, mitoplasts (outside-out submitochondrial vesicles lacking the outer membrane) prepared from HEK 293 cells are incubated with a 5-kDa, membrane impermeant thiol-reactive reagent, polyethylene glycol maleimide (PEGM). If the protein of interest has a cysteine exposed to the external solution, PEGM would react with this cysteine and thus increase the protein’s molecular weight.
We first validated the assay on the known orientation of MCU (Kamer and Mootha, 2015; Murgia and Rizzuto, 2015). Human MCU possesses five cysteines, all in the N-terminal domain. If this region faces outward towards the IMS, PEGM would increase MCU’s mass by 5 kDa per residue modified. Experiments (Figure 2A), however, show that MCU mobility on SDS-PAGE is not altered by PEGM treatment unless the mitoplast membrane is first disrupted by the mild detergent dodecyl maltoside (DDM). The results thus confirm MCU’s Nin-Cin orientation, with both the N- and C-termini residing in the matrix.
To determine EMRE topology, we constructed mutants with unique cysteines (I49C or I97C) engineered on either side of the TMH of the naturally cysteineless EMRE. Like WT, these mutants support MCU-dependent Ca2+ flux in EMRE-KO cells (Figure 2—figure supplement 1). In intact mitoplasts free of native EMRE protein, PEGM readily labels I97C-EMRE but fails to react with I49C or the cysteineless WT protein, while after detergent pretreatment both mutants are labeled (Figure 2B). EMRE thus adopts a Nin-Cout orientation, with its C-terminal tail facing the IMS.
Figure 2C summarizes the inner-membrane topology of MCU and EMRE inferred here. This was further verified by fusing EMRE onto the C-terminus of MCU, thus forcing the orientation of the two proteins in tandem to conform to the above molecular picture (Figure 2C). The fusion construct was tested in ME-KO cells, where it was expressed as a full-length protein detectable by both MCU and EMRE antibodies (Figure 2D). As with WT MCU, PEGM fails to modify any of the five native cysteines in the N-terminus without detergent pretreatment (Figure 2E), thus implying that the fusion protein is inserted homogeneously into the inner membrane in a proper Nin-Cout orientation. Moreover, the MCU-EMRE fusion protein mediates robust, Ru360-sensitive mitochondrial Ca2+ uptake (Figure 2F), a powerful result corroborating the transmembrane orientation cartooned in Figure 2C.
We next investigate how EMRE interacts with MCU to support Ca2+ permeation. This issue is addressed using three EMRE variants (Figure 3A), with N- or C-termini largely deleted by replacing it with a foreign 'C8' epitope (PRGPDRPEGIEE) (Abacioglu et al., 1994) into either region, or with the TMH substituted by an artificial transmembrane 'WALP' helix (GWWLALALALALALALWWA) (Killian et al., 1996). These mutants, named ΔN-, ΔC-, or WALP-EMRE, are all properly targeted to EMRE-KO mitochondria (Figure 3B). Both ΔN- and ΔC-EMRE fully support uniporter function, but cells expressing WALP-EMRE exhibit no uniporter activity (Figure 3C). The results are surprising, as the strict conservation of EMRE’s C-terminal polyaspartate tail implies that it should carry out some sort of crucial function.
To locate EMRE residues critical for MCU-mediated Ca2+ transport, we performed tryptophan scanning mutagenesis to cover the entire transmembrane region (S64 to A92). This classical strategy posits that the large tryptophan side chain introduced at a protein interface is more likely to disrupt helical packing and hence function than when projecting towards lipid (Hong and Miller, 2000; Sharp et al., 1995). The results (Figure 3D, Figure 3—figure supplement 1) highlight a rather clean segregation of Trp-sensitive vs insensitive positions on a helical-wheel diagram. In particular, Trp substitution at G81 or S85 completely eliminates Ca2+ uptake via the uniporter complex. These two residues belong to a conserved Gxxx[G/A/S] motif (Figure 3E), frequently found to mediate packing of TMHs in membrane protein structures (Russ and Engelman, 2000).
Co-immunoprecipitation (co-IP) experiments further confirm the GxxxS sequence as crucial to MCU-EMRE complex formation. EMRE variants were co-expressed with MCU carrying a C-terminal '1D4' epitope (TETSQVAPA) (MacKenzie et al., 1984) in ME-KO cells, and the MCU-EMRE complex was immobilized on a 1D4 affinity column for downstream analysis. WT EMRE is captured by MCU, but disruption of the GxxxS region by the G81W or S85W mutation prevents this association, while Trp substitution of residues elsewhere on the TMH does not (Figure 3F). These results taken together show that EMRE binds to MCU using a Gxxx[G/A/S] motif in the C-terminal half of its TMH, and that this binding is necessary to activate the Ca2+-conducting pore in the uniporter complex.
To examine the MCU side of the interaction with EMRE, we launched Trp-perturbation mutagenesis of both TMHs (residues L234 – T254 in TMH1; Y268 – V283 in TMH2). All mutants were expressed to near WT levels in MCU-KO cells (Figure 4—figure supplement 1), and were classified as either low- or high-impact on Ca2+ transport function (Figure 4A, Figure 4—figure supplement 1). Of the 16 Trp mutations in TMH2, only two (F269W, T271W, located near the N-terminal end of the helix) induce severe functional defects, as if most TMH2 residues project either to the lipid bilayer or an aqueous cavity. In contrast, the six Trp-sensitive positions in TMH1 tend toward one side of a helical wheel diagram, suggesting that this high-impact face might pack against other TMHs in the MCU-EMRE complex.
If a Trp mutation in MCU perturbs the function of human uniporter solely by interrupting EMRE binding, it should cause negligible functional impact when introduced at corresponding positions in D. discoideum MCU, because this homologue transports Ca2+ without EMRE (Figure 1C). We therefore introduced each of the eight high-impact Trp substitutions (Figure 4A) into D. discoideum MCU to test the mutational effect in ME-KO cells. The results (Figure 4—figure supplement 2) show that six of these mutations are functionally disruptive while two are fully active; these two active mutants correspond to L240W and A241W in TMH1 of human MCU, suggesting that these residues in human MCU contact EMRE.
A steric clash between a substituted Trp in MCU and a native residue in EMRE could in principle be alleviated by reducing the side-chain volume of that particular EMRE residue. We chose F77 in EMRE to test this idea, as it is on the same helical face as the GxxxS sequence identified above, and since the large Phe residue enables substantial shortening of the side chain. Accordingly, F77A EMRE was coexpressed with each of the 6 functionally defective MCU Trp mutants of TMH1. This EMRE mutant, which forms a functional channel with WT MCU, rescues Ca2+ transport with A241W but not with any of the other mutants (Figure 4—figure supplement 3). Similarly, the impaired uniporter function induced by A241F in MCU is rescued by F77A in EMRE. Co-IP experiments further show that A241F (or A241W) MCU pulls down F77A but not WT EMRE (Figure 4C, Figure 4—figure supplement 3). These results demonstrate that the combination of a large and a small side chain (F or W, A) on EMRE position 77 and MCU position 241 leads to proper transport function regardless of which protein the residues occupy. This 'side chain swap' experiment argues strongly that A241 in MCU’s TMH1 is in close proximity to F77 in the TMH of EMRE in the uniporter complex.
While analyzing MCU/EMRE from various species (Figure 1C), we noticed that human MCU (hMCU) forms functional Ca2+ channels with human EMRE (hEMRE) or with C. elegans EMRE (ceEMRE), but C. elegans MCU (ceMCU) supports mitochondrial Ca2+ uptake only with ceEMRE (Figure 4D). These results present an opportunity to test which of ceMCU’s two TMHs is responsible for discriminating against hEMRE. Accordingly, we produced two MCU chimeras, with TMH1 or TMH2 of ceMCU substituted by the corresponding region in hMCU. The chimera containing hMCU TMH1, though well expressed, is transport-inactive in the presence of either hEMRE or ceEMRE. The chimera containing hMCU TMH2, however, is activated by ceEMRE, while remaining unresponsive to hEMRE (Figure 4D). This result independently supports the proposal that TMH1 of MCU contains the interaction region for EMRE.
With the membrane disposition of the MCU-EMRE complex in hand, we now ask how EMRE interacts with MICU proteins (Sancak et al., 2013), which contain no apparent transmembrane sequences. To tackle this problem, we began by testing whether MICUs bind to EMRE from the matrix- or IMS-side of the inner membrane. Currently, the submitochondrial localization of MICU1 is unsettled (Csordas et al., 2013; Foskett and Madesh, 2014; Hoffman et al., 2013; Hung et al., 2014; Waldeck-Weiermair et al., 2015), and that of MICU2 has not been approached with rigorous methods (Vais et al., 2016). Moreover, although MICU1 is known to be a peripheral membrane protein (Csordas et al., 2013), it remains unclear if MICU2 is similarly attached to the inner membrane.
Inner membrane association of MICUs was probed by stripping mitoplasts of peripheral membrane proteins using alkaline Na2CO3 treatments. Figure 5A shows that MICU1 and cytochrome C (Cyt-C), but not the integral membrane protein Letm1, are extracted into Na2CO3 solution, a result consistent with a previous report (Csordas et al., 2013) that MICU1 is a peripheral membrane protein. Furthermore, nearly all MICU1 is membrane-bound, virtually none appearing in the IMS without Na2CO3 treatment, in contrast to Cyt-C, which is found in both the IMS and the membrane (Figure 5A). Similar experiments demonstrate that MICU2 remains membrane-associated even after harsher Na2CO3 extraction conditions (Figure 5B). The results thus establish that MICU1 and MICU2 are both confined to the inner-membrane surface under physiological conditions.
To test if MICU1 is exposed to the mitochondrial matrix or the IMS, we incubated mitoplasts with PEGM, relying on MICU1's native cysteines to report accessibility to the reagent from the mitoplast exterior. PEGM readily reacts with MICU1 (Figure 5C), showing that this subunit resides on the outer, IMS side of the inner membrane. Two issues regarding this experiment require comment. First, the PEGM-treated sample appears as a smear instead of a defined band, consistent with heterogeneous modification of the protein's 7 native cysteines. Second, a significant western-blot signal is observed roughly corresponding to MICU1 dimers, stable in SDS-PAGE conditions, as reported previously (Patron et al., 2014). This signal also shifts upward after PEGM treatment, suggesting that MICU1 dimers or higher oligomers are also localized to the IMS. Similar results in parallel experiments with MICU2 (Figure 5D) argue that both MICU1 and MICU2 are associated with the outer leaflet of the mitochondrial inner membrane, a location in harmony with the cytoplasmic Ca2+-sensing function of these proteins.
It has been established, as we also confirm here (Figure 5—figure supplement 1), that MICU1 forms a stable complex with MICU2 (Kamer and Mootha, 2014; Patron et al., 2014), and that MICU1, but not MICU2, is tightly associated with the MCU-EMRE complex (Kamer and Mootha, 2014; Sancak et al., 2013). It however remains uncertain if MICU1 interacts with MCU or EMRE (Hoffman et al., 2013; Sancak et al., 2013), a problem addressed using co-IP to examine association of FLAG-tagged MICU1 with MCU or EMRE expressed individually in ME-KO cells. As illustrated in Figure 5E, MICU1 can precipitate EMRE without MCU present and MCU without EMRE present. This observation rules out a required MICU1-interacting surface contributed by both MCU and EMRE. It also invites us to search for the molecular determinants mediating MICU1-EMRE interaction in the absence of MCU.
The IMS localization of MICU1 (Figure 5C) implies that EMRE binds to MICU1 via its C-terminal, IMS-exposed region containing the polyaspartate tail (EDDDDDD). This highly charged tail alerts us to a complementary polybasic sequence (KKKKR), which though conserved in MICU1, is absent in MICU2 (Hoffman et al., 2013). Indeed, co-IP experiments demonstrate that MICU1 pulls down ΔN- but not ΔC-EMRE (Figure 6A). Moreover, a MICU1 mutant carrying an electrostatically neutered sequence (KKKKR =>EQEQR) readily complexes with MICU2, but not with EMRE (Figure 6B). These results strongly argue that the EMRE-MICU1 interaction is mediated by this electrostatic pair. The strict conservation of these charged sequences further suggests that the EMRE-MICU1 interaction plays an important, previously unappreciated physiological role.
The MICU proteins act as the Ca2+-sensing gate in the uniporter complex, shutting the pore at resting cellular Ca2+ concentrations and opening it when cytoplasmic Ca2+ exceeds ~1 µM. By binding to both MCU and MICU1, EMRE might serve as an anchor to retain the MICU1-MICU2 pair near the Ca2+-conducting pore. If so, disrupting the MICU1-EMRE interaction would yield a population of channels free of MICUs, allowing unregulated, constitutive Ca2+ permeation from the cytosol into the matrix. To examine this idea, we quantify MCU-dependent Ca2+ uptake by following accumulation of the 45Ca2+ radioisotope into mitochondria in digitonin-permeabilized cells, an approach that allows free extramitochondrial Ca2+ to be buffered at well-defined submicromolar concentrations without sacrificing sensitivity.
We first examine the effect of MICU1 knockdown (KD) on mitochondrial Ca2+ uptake. Three HEK293 cell lines stably expressing distinct short hairpin RNAs (sh1 – 3) were generated, all exhibiting at least 70% decrease of MICU1 mRNA and normal levels of MCU or EMRE protein (Figure 6—figure supplement 1). Although insufficiently sensitive antibodies frustrate quantification of MICU1, the MICU1-KD cell lines show a profound functional alteration in 45Ca2+ uptake. At high Ca2+ (30 μM), rates of Ca2+ transport into WT and MICU1-KD mitochondria are similar, while as expected, uptake is virtually undetectable in ME-KO cells (Figure 6C). At low Ca2+ (0.5 μM), however, MICU1-KD mitochondria show massive Ca2+ accumulation in the matrix (Figure 6D), in dramatic contrast to WT, where very little Ca2+ uptake occurs. These observations confirm previous studies that identified MICU1 as a molecular element controlling Ca2+-dependent activation of the uniporter complex (Csordas et al., 2013; Mallilankaraman et al., 2012).
Functional manifestations of the EMRE-MICU1 interaction were further examined by comparing 45Ca2+ uptake supported by EMRE variants expressed in EMRE-KO cells. As above, WT-, ΔN-, or ΔC-EMRE all activate MCU-dependent Ca2+ uptake to a similar degree at high Ca2+ (Figure 6E). At low Ca2+, uptake is suppressed in mitochondria hosting WT or ΔN-EMRE, but is enhanced over 50-fold by MICU1 knockdown (Figure 6F). In contrast, Ca2+ rapidly enters mitochondria containing ΔC-EMRE, which cannot bind MICU1, and the rate is only trivially increased after MICU1 KD (Figure 6G). We thus conclude that MICU1 must bind EMRE to maintain uninterrupted engagement with the MCU pore, thus conferring Ca2+-dependent gating upon what would otherwise be constitutive Ca2+ leakage into the mitochondrial matrix.
In the past few years, the mitochondrial Ca2+ transport field has witnessed a molecular dawn following a half-century of functional phenomenology. It is now firmly established that the human mitochondrial Ca2+ uniporter is a Ca2+-activated Ca2+ channel composed of at least four proteins: MCU, EMRE, MICU1, and MICU2. The present work focuses mainly on EMRE, the least understood component of the channel complex. Impressed by the functional dependence on EMRE arising as metazoan uniporters evolved, we have endeavored to enrich our current view of the uniporter subunits and the physiological purposes of the domain interactions mediating their assembly. Results here establish (1) EMRE orientation in the inner membrane, (2) the molecular contacts governing EMRE interactions with MCU and MICU1, (3) the disposition of the MICU1-MICU2 complex on the outer surface of the inner-membrane, and (4) the functional purpose of the EMRE-MICU1 interaction. These findings lead to a molecular model (Figure 7) featuring a central role of EMRE in orchestrating uniporter responses to intracellular Ca2+ signaling.
When a Ca2+ channel in the plasma membrane opens, or when an intracellular store releases Ca2+, a cytoplasmic Ca2+ wave is generated. Once the wave hits mitochondria, Ca2+ can rise above ~1 μM, activating the uniporter to catalyze rapid Ca2+ entry into the mitochondrial matrix. Mitochondria can therefore serve as a buffer to shape intracellular Ca2+ signals (Demaurex et al., 2009). Moreover, Ca2+ entry boosts ATP output by accelerating the citric acid cycle, but excessive, sustained Ca2+ accumulation in the matrix triggers caspase-dependent apoptosis (Rizzuto et al., 2012). Thus, mitochondria can also decode Ca2+ stimulation as either metabolic or death signals. Failure of the uniporter to appropriately respond to Ca2+ signals would perturb these crucial physiological processes and could also produce other serious problems. For instance, if the uniporter fails to stay inactive under resting conditions, the large negative inner membrane potential of energized mitochondria would drive continual Ca2+ influx. Removing these Ca2+ ions requires the action of Na+/Ca2+ and Na+/H+ exchangers at a cost of 3 H+ entering into the matrix for each Ca2+ expelled. Unregulated, ‘leaky’ uniporters would therefore divert protons away from the FoF1-ATPase, partially uncoupling electron transport from ATP synthesis.
The recent discovery of MICU proteins (Baughman et al., 2011; Mallilankaraman et al., 2012) has enhanced our mechanistic understanding of how cellular Ca2+ signals control uniporter activity. It is now clear that the mitochondrial response to physiological Ca2+ is mediated by the Ca2+-sensing gate formed by the MICU subunits that engage the pore-lining MCU proteins at the external face of the inner membrane. This modular design comes with a potential danger: that the gating and ion-conducing subunits might dissociate sporadically, producing unregulated Ca2+ channels. How could this problem be prevented? It is likely that MICU1 has a high intrinsic affinity to MCU, since at least part of the MICU1-MCU complex survives lengthy co-IP experiments. Moreover, diffusion of MICUs confined to two dimensions within the inner membrane raises the local density of MICUs near the MCU pore. These factors combined could in principle reduce the population of MICU-free, unregulated uniporters — a condition that might be particularly helpful to protist and plant mitochondria, where MCU and MICUs are the only components of the uniporter complex.
The small, single-pass membrane protein EMRE emerges in animals as a new subunit of the uniporter complex. A crucial finding here is that the presence of EMRE in the uniporter complex ensures that the channel conducts Ca2+ only when cytoplasmic Ca2+ rises above resting levels. This requires EMRE to use its polyaspartate tail to bind MICU1, as if it functions as 'molecular glue' to prevent dissociation of MICU1 from the MCU pore, a circumstance that would produce catastrophic Ca2+ leakage (Figure 7). Alternatively, EMRE might allosterically transmit the Ca2+ signal from MICUs to the pore; in this case, disrupting EMRE-MICU1 interaction would also prevent MICUs from properly gating the Ca2+ pathway. We consider this allosteric scenario unlikely, as the mechanism by which MICU1 gates MCU probably evolved in early eukaryotic evolution when EMRE was absent.
The understanding that EMRE safeguards mitochondria against inappropriate Ca2+ uptake helps us appreciate the physiological importance of the strict EMRE-dependence of uniporter function appearing in animals. As in any multisubunit protein, it is inevitable that EMRE might occasionally dissociate from MCU, and some tissues under natural or pathological conditions might express MCU in excess of EMRE. Under these situations, a population of EMRE-free uniporters could arise. These channels would also lack MICUs, which would no longer be EMRE-linked to the pore. But the MCU-activation function of EMRE would ensure that these channels would become inactive, preventing them from wreaking havoc on normal cell physiology (Figure 7).
We should point out that our results clash with several published assertions regarding the uniporter complex. First, single-channel recordings in planar lipid bilayers have been used to argue that the human MCU protein alone is sufficient to reconstitute a Ca2+ channel without EMRE (De Stefani et al., 2011; Patron et al., 2014). These recordings, however, obtained with in vitro-expressed protein of uncharacterized purity, show channel properties vastly different from uniporter currents directly patch-recorded from intact mitoplasts (Kirichok et al., 2004). Second, a recent study (Vais et al., 2016) using protease digestion argues that EMRE adopts a Nout-Cin orientation, opposite to that deduced here. Interpretation of the assay, however, is based on an unjustified assumption that the N- and C- termini of EMRE are digested at similar rates. In contrast, our results are supported by two lines of direct and independent evidence – mass tagging of substituted cysteines and the functional competence of the MCU-EMRE fusion protein (Figure 1). This same study also claims that EMRE uses its C-terminal tail to sense matrix Ca2+, producing a biphasic response of uniporter activity to matrix Ca2+. However, this phenomenon, to our best knowledge, has not been observed in any mitochondrial Ca2+ uptake experiments in the literature or in previous patch recordings (Kirichok et al., 2004). We also note a recent study appearing when our work was under review (Yamamoto et al., 2016) that deduced, using an epitope-tagging method, a Nin-Cout EMRE orientation fully consistent with our results. That study also shows that a Pro-to-Ala substitution 3 residues N-terminal to the predicted TMH - a region left unperturbed in our 22-residue deletion ΔN-EMRE construct - abolishes EMRE-MCU interaction. Thus, a small portion in the N-terminus of EMRE near the TMH might also be involved in binding MCU.
In summary, our results argue that EMRE mediates two distinct functions – MCU activation and MICU retention - through two distinct types of subunit-subunit interactions. These functions conspire to achieve a single physiological outcome: obligatory linkage of the Ca2+-conducting and Ca2+-sensing machineries, a necessary condition for the uniporter complex to respond rapidly and accurately to the elaborate Ca2+-signaling network that has evolved in animal cells.
Site-directed mutagenesis was performed using the QuickChange mutagenesis kit (Agilent). HEK 293 cells were grown in Dulbecco’s modified Eagle’s medium supplemented with 10% FBS, and were incubated at 37°C, 5% CO2. The HEK-293 cell line was supplied by Dr. D.E. Clapham and authenticated by short tandem repeat profiling conducted by ATCC, and was free of mycoplasma as determined by PCR based detection using a kit supplied by ATCC (30-1012K). Transient transfection was performed using Lipofectamine 3000 (Life Technologies), following manufacturer’s instructions. Cells were used for downstream analysis 1-2 days after transfection.
Stable knockdown was achieved by lentivirus, using the transfer vector pLKO.1 puro (Sigma) for U6-driven shRNA expression. The viral titer was determined with a p24 ELISA kit (Clontech, Mountain View, CA). WT HEK293 cells were exposed to the virus for 12 hr, using a multiplicity of infection of 5–10. Afterwards, the culture was incubated with 2 μg/mL puromycin for 2 days to eliminate untransduced cells. The efficiency of knockdown was evaluated by quantitative PCR (qPCR). Detailed qPCR procedure and the shRNA sequences are reported in Extended Experimental Procedures.
Gene knockout by CRISPR/Cas9 was performed using the published protocol (Ran et al., 2013). In brief, the pSpCas9(BB) vector containing the 20-nucleotide guide sequence was transfected into HEK 293 cells. After two days of incubation, single cells were isolated by serial dilutions, and expanded for 2–4 weeks. Gene KO was assessed by sequencing and Western blot. Two sets of guide sequences (see Supplementary file 1) were used to rule out off-target effects.
For Western blot, proteins on an SDS gel were transferred onto nitrocellulose membranes, which were blocked by 5% milk in TBS, and then incubated with the primary antibody diluted in TBST (TBS + 0.1% Tween-20). Signal development was done using alkaline phosphatase conjugated secondary antibodies (Pierce) and the NBT/BCIP substrate (Life Technologies). The primary antibody and dilution used: α-MCU (Sigma, HPA016480, 1:2000), α-EMRE (Santa Cruz, 86337, 1:400), α-FLAG (Sigma, F1804, 1:4000), α-V5 (Life Technologies, 46–0705, 1:5000), α-Cyt-C (Santa Cruz, 13156, 1:1000), α-β-actin (Santa Cruz, 69879, 1:500), α-Letm1 (Abcam, 55434, 1:2000). Monoclonal anti-1D4 and -C8 antibodies were produced in house.
All co-IP experiments were performed at 4 °C. Transfected HEK 293 cells were grown in a 10-cm dish to confluency, were harvested, and then lysed in 1-mL solubilization buffer (SB, 100 mM NaCl, 20 mM Tris, 1 mM EGTA, 25 mM DDM, pH 7.5-HCl), supplemented with an EDTA-free protease inhibitor cocktail (cOmplete Ultra, Roche). The cell lysate was clarified by centrifugation, and a small portion of the sample was taken for whole cell lysate analysis. Antibody-conjugated Sepharose beads (25 μL) were added, and after 1 h, the beads were collected on a mini column, washed with 2-mL SB, and eluted with 200-μL SDS-gel loading buffer for Western blot. Antibody affinity gel used: FLAG (Sigma, A2220), V5 (Sigma, A7345). 1D4 and C8 affinity gels were produced using 20-mg 1D4 or C8 antibody per 1-g Sepharose 4B (GE Healthcare).
Mitoplasts were formed at 4°C by standard procedures that yield outside-out, stable transport vesicles. Protease inhibitor (cOmplete Ultra, Roche) was present in all steps. HEK 293 cells from a 15-cm dish were pelleted, resuspended in 2-mL mitochondria resuspension buffer (MRB, 250 mM sucrose, 5 mM HEPES, 1 mM EGTA, pH 7.2-KOH), and lysed by passing through a 27.5 g needle 15 – 20 times. Nuclei and cell debris were removed by spinning the cell lysate at 1000 g for 10 min. The supernatant was spun down at 10,000 g for 10 min, resuspended in 2-mL MRB, and then spun down again to pellet crude mitochondria. To obtain mitoplasts, mitochondria were resuspended in 800-μL hypotonic shock buffer (5 mM sucrose, 5 mM HEPES, 1mM EGTA, pH 7.2-KOH), and subjected to osmotic shock for 10 min. Then 200 μL of high-salt storage buffer (750 mM KCl, 100 mM HEPES, 2.5 mM EGTA, pH 7.2-KOH) was added, and mitoplasts were subsequently sedimented by centrifugation at 20,000 g for 10 min. The supernatant, which contains proteins in the outer membrane and the intermembrane space, was collected if further analysis is required.
Mitoplasts were resuspended in the thiol-modification buffer (100 mM NaCl, 50 mM MOPS, pH 7.0-NaOH), to which 1 mM PEGM (Sigma) in the presence or absence of 1 mM DDM (Anatrace) was added. The samples were incubated for 1–4 hr at RT before the reaction was quenched with 5 mM cysteine. All reagents were prepared fresh before experiments.
Mitoplasts were resuspended either in carbonate extraction buffer (120 mM NaCO3, pH 10.5- or 11.5-NaOH) or in a control solution (250 mM sucrose, 25 mM Tris, pH 7.0-HCl). The samples were incubated at 4°C or RT for 1 hr, and then spun down with ultracentrifugation at 200,000 g for 1 hr. The supernatant contains proteins extracted by carbonate, while the membrane pellet containing integral membrane proteins.
All Ca2+ uptake assays were repeated at least 3 times on multiple preparations, and traces in figures show typical responses. For the fluorescence-based assay, 107 HEK 293 cells were suspended in 10-mL Ca2+ flux wash buffer (CWB, 120 mM KCl, 25 mM HEPES, 2 mM KH2PO4, 1 mM MgCl2, 50 μM EGTA, pH 7.2-KOH), pelleted at 1000 g for 5 min, and resuspended in 2.5-mL recording buffer (RB, 120 mM KCl, 25 mM HEPES, 2 mM KH2PO4, 1 mM MgCl2, 5 mM succinate, pH 7.2-KOH). 2 mL of the cell suspension was loaded into a stirred quartz cuvette in a Hitachi F-2500 spectrophotometer (ex: 506 nm, ex-slit: 2.5 nm, em: 532 nm, em-slit: 2.5 nm, sampling frequency: 2 Hz), with the temperature maintained at 37°C by a circulating bath. In a typical experiment, reagents were added into the cuvette in the following order: 0.5-μM calcium green 5N (Life Technologies), 30-μM digitonin (Sigma), 10-μM CaCl2, and 2-μM Ru360 (Santa Cruz). Under these conditions, peak free Ca2+ concentrations were close to 10 μM (11 + 6 SD, N=40, as determined by calibration). Because of uncertainties in protein concentration, Ca2+ uptake activity is reported as a linear fit to the fluorescent signal obtained in the first 10 s after addition of 10-μM CaCl2. Activity was not altered by 5 µM thapsigargin.
For the 45Ca2+ based uptake assay, 2 x 106 cells were suspended in 1.5-mL CWB, spun down at 2,000 g for 1 min, and resuspended again in 200-μL CWB, supplemented with 5 μM thapsigargin and 30 μM digitonin. To initiate Ca2+ flux, 100 μL of the cell suspension was transferred to either 400-μL high-Ca2+ flux buffer (RB + 10 μM EGTA and 40 μM 45CaCl2) or 400-μL low-Ca2+ flux buffer (RB + 0.69 mM EGTA, 0.5 mM CaCl2, and 20 uM 45CaCl2, pH 7.2-KOH), with both solution containing 5-μM thapsigargin and 30-μM digitonin. At desired time points, 100 μL of the reaction mixture was added into 5-mL ice-cold CWB, and then filtered through 0.45-μm nitrocellulose membranes (EMD-Millipore) on a vacuum filtration manifold (Millipore model 1225). The membrane was washed immediately with 5-mL ice cold CWB, and later transferred into scintillation vials for counting. 45Ca2+ radioisotope was purchased from Perkin Elmer, with a specific activity of 12.5 mCi/mg.
Sequences of MCU or EMRE homologues were collected using PSI-BLAST search of ~100 species. EMRE was identified by the presence of the polyaspartic tail, and MCU by the conserved DIME loop. Multiple sequence alignment was performed using the ClustalW2 online server (Larkin et al., 2007). The helical wheels were plotted using Antheprot v 6.4 (Deleage et al., 2001). Mitochondrial targeting sequence prediction was carried out using the TargetP 1.1 online server (Emanuelsson et al., 2007).
Whole cell RNA was extracted from HEK 293 cells grown in 6-well plates using TRIzol. Residual DNA was removed using the TURBO DNA-free kit (Life Technologies). First strand cDNA synthesis was performed with 1 μg RNA using M-MuLV reverse transcriptase (NEB), following manufacturer’s instructions. The sample was subsequently digested with RNaseH (NEB). qPCR was performed with SsoFast EvaGreen Supermixes (BIO-RAD), using 0.5 μM β-actin or MICU1 primers, and 0.5, 2.5, 5, or 10 ng RNA for producing a standard curve. Detection of the PCR product was done with a CFX96 real-time PCR detection system (BIO-RAD), using the following protocol: 95°C for 30 s, 50 cycles of 95°C for 5 s and 57°C for 5 s. The sequence of the primers is provided in Supplementary file 1.
ΔCt was calculated by subtracting the Ct for β-actin from the Ct for MICU1, with 3 independent RNA extractions and qPCR measurements using 2.5 ng whole RNA. ΔΔCt was calculated by subtracting the mean ΔCt for control WT cells from the mean ΔCt for each stable MICU1 knockdown cells. The results were presented as the percentage of MICU1 mRNA in MICU1 knockdown cells relative to MICU1 mRNA in WT control, using the equation% mRNA = 1/2|ΔΔCt|.
Epitope mapping and topology of baculovirus-expressed HIV-1 gp160 determined with a panel of murine monoclonal antibodiesAIDS Research and Human Retroviruses 10:371–381.https://doi.org/10.1089/aid.1994.10.371
Evolutionary diversity of the mitochondrial calcium uniporterScience, 336, 10.1126/science.1214977, 22605770.
A survey of the interaction of calcium ions with mitochondria from different tissues and speciesThe Biochemical Journal 122:681–690.https://doi.org/10.1042/bj1220681
ANTHEPROT: An integrated protein sequence analysis software with client/server capabilitiesComputers in Biology and Medicine 31:259–267.https://doi.org/10.1016/S0010-4825(01)00008-7
Regulation of plasma membrane calcium fluxes by mitochondriaBiochimica Et Biophysica Acta 1787:1383–1394.https://doi.org/10.1016/j.bbabio.2008.12.012
Regulation of the mitochondrial Ca(2+) uniporter by MICU1 and MICU2Biochemical and Biophysical Research Communications 449:377–383.https://doi.org/10.1016/j.bbrc.2014.04.146
The lipid-protein interface of a shaker K(+) channelThe Journal of General Physiology 115:51–58.https://doi.org/10.1085/jgp.115.1.51
The molecular era of the mitochondrial calcium uniporterNature Reviews. Molecular Cell Biology 16:545–553.https://doi.org/10.1038/nrm4039
Reconstitution of the mitochondrial calcium uniporter in yeastProceedings of the National Academy of Sciences of the United States of America 111:8985–8990.https://doi.org/10.1073/pnas.1400514111
Mitochondria as sensors and regulators of calcium signallingNature Reviews. Molecular Cell Biology 13:566–578.https://doi.org/10.1038/nrm3412
The gxxxg motif: A framework for transmembrane helix-helix associationJournal of Molecular Biology 296:911–919.https://doi.org/10.1006/jmbi.1999.3489
Features of mota proton channel structure revealed by tryptophan-scanning mutagenesisProceedings of the National Academy of Sciences of the United States of America 92:7946–7950.https://doi.org/10.1073/pnas.92.17.7946
EMRE is a matrix Ca(2+) sensor that governs gatekeeping of the mitochondrial Ca(2+) uniporterCell Reports, 14, 10.1016/j.celrep.2015.12.054, 26774479.
Rearrangement of MICU1 multimers for activation of MCU is solely controlled by cytosolic Ca(2.)Scientific Reports, 5, 10.1038/srep15602, 26489515.
Analysis of the structure and function of EMRE in a yeast expression systemBiochimica Et Biophysica Acta (BBA) - Bioenergetics, 10.1016/j.bbabio.2016.03.019.
David E ClaphamReviewing Editor; Howard Hughes Medical Institute, Boston Children's Hospital, United States
In the interests of transparency, eLife includes the editorial decision letter and accompanying author responses. A lightly edited version of the letter sent to the authors after peer review is shown, indicating the most substantive concerns; minor comments are not usually included.
Thank you for submitting your article "A Central Role of EMRE in Regulation of the Mitochondrial Uniporter Complex" for consideration by eLife. Your article has been favorably evaluated by Gary Westbrook (Senior Editor) and three reviewers, one of whom, David Clapham, is a member of our Board of Reviewing Editors. The reviewers discussed the reviews with one another and the Senior Editor has drafted this decision to help you prepare a revised submission.
As you will see, all reviewers were enthusiastic about the quality and relevance of the work, and its publication after revision in eLife. Reviewers 2 and 3 had very minor points as listed below. Reviewer 2 made some extensive comments, but note these were considered "suggestions". We ask that you consider these points in your revised manuscript.
Minor points from Reviewers 2 and 3:
1) A new paper in BBA reports functional mutagenesis of EMRE, showing that deletion of amino acids at EMRE's N terminus abolishes function, in conflict with the current paper. The discrepancy ought to be reconciled or at least discussed.
2) Figure 2B: Western blot: what was the molecular weight for EMRE?
3) Figure 5C and D: The predicted mol. wt. for MICU1 and MICU2 are approximately 54 and 50 KDa respectively. The observed bands in Western blots are near 64 KDa for both. How do you explain the difference? You also mentioned that MICU1 antibody was not good enough to quantify the MICU1 expression level in MICU1 knockdown cell lines. A comment on reliability of your MICU1 antibodies may be needed.
4) Figure 5B indicates that MICU2 remains membrane-bound even after harsher Na2CO3 treatment where as MICU1 comes off. This result seems to contradict the reports indicating that MICU2 binds MCU-complex via MICU1 either by forming a disulfide bond or other (Patron et al, 2014 as well as Kamer et al, 2014). This result was not discussed at all considering its importance.
5) Figure 5E shows that MICU1 can precipitate MCU in the absence of EMRE. Doesn't this observation contradict the strict requirement of EMRE C-terminal for MICU1 engagement with the pore-forming subunit (MCU)?
Reviewer #1 general assessment:
Tsai et al. investigate EMRE, a non- pore-forming subunit of the mitochondrial uniporter, (the MCU, or MiCa channel). They approach the relevant orientation and functional questions with a large number of experimental methods. First, the authors use gene editing to develop assays in permeabilized HEK cells. MCU homologs from amoeba and plants mediate Ca uptake alone, while worms, flies, and human MCU requires EMRE. Cysteine and tryptophan scanning/substitution mutagenesis then enables them to verify that MCU's N and C termini are inside the mitoplast, while EMRE's N terminus is inside the mitoplast and its C terminus outside. Chimeras and other experiments then suggest that TMH1 of MCU is the interaction region for EMRE. Other subunits, the relatively soluble subunits MICU1 and MICU2, are shown to associate with the outer leaflet of the inner mitochondrial membrane, and that EMRE associates with MICU1 by electrostatic interactions. MICU function is verified as regulating Ca entry, and the authors propose that MICU1 must bind EMRE to engage the MCU pore. Thus, MICUs regulate what would otherwise be an unregulated Ca pore. An important finding is that EMRE polyaspartate tail ensures that the channel conducts Ca only when cytoplasmic Ca rises above its resting levels. In short, EMRE mediates MICU retention and MCU retention. Based on the lack of EMRE in lower species, the authors conclude that EMRE arose as a safety mechanism, allowing MICU1 gating of MCU only if EMRE is present.
This work clarifies many of the issues that have plagued MCU subunit function and orientation. The methods are robust and the conclusions are likely sound. The only additional experiments the field needs to settle remaining conformational questions is the high resolution structure of the MCU/EMRE/MICU1/2/MCUR1 complex.
Reviewer #2 general assessment and major comments:
This is a new paper from a premiere ion channel biochemistry lab. The paper focuses on the macromolecular organization of the mitochondrial calcium uniporter complex, which in humans consists of five proteins: MCU (the pore), MICU1/2 (calcium sensors in the IMS), and EMRE (a small membrane protein that relays MICU1/2 calcium sensing to the open state of MCU). The study combines clever biochemical methods (e.g., artificial transmembrane domains, tryptophan mutagenesis, and protein fusions) to shed insight into the complex, with a focus on EMRE and its interactions with MCU and MICU1. The topic is timely. Some important suggestions designed to help the authors improve what could be a very important paper:
1) This paper reports a mix of novel and not so novel results – hence novel findings are buried. I would encourage the authors to emphasize the novel and de-emphasize results that are confirmatory. For example, the topology of MCU, IMS localization of MICU1/2, gatekeeping activity by MICU1, and dual functions of EMRE (keeping MCU open and linking MICU1/2 to MCU) are all well-established now. The novel findings include the topology of EMRE, the MCU-EMRE fusion (which could be a very important tool for the field), EMRE interaction with TM1, and interaction between MICU1 and EMRE.
2) In MCU knockout cell lines and mouse tissues, loss of MCU causes loss of EMRE expression. In many figures, the authors claim loss of interaction (by IP) between EMRE and MCU, but EMRE protein expression seems normal. This raises the possibility that the IP conditions used here do not reflect the in vivo binding status of these two proteins. For example, in Figure 3F, the authors mutate EMRE and evaluate which variants are able to interact with MCU. The S85W mutant evidently expresses very well, yet does not interact with MCU. Can the authors provide additional evidence, e.g., BN-PAGE, demonstrating a lack of a shift of the MCU holocomplex?
3) The authors claim that the δ-N, δ-C and WALP EMRE localize to the mitochondria. However, it's not clear how pure their mitochondrial preps are without markers for other organelles. Alternatively, they could consider simple microscopy experiments to look for co-localization with mitochondrial markers. Again, it is surprising to see that WALP EMRE expresses so well, even though it does not bind to MCU. How do the activities of δ-N and δ-C compare to WT? Please add WT EMRE traces and expression to Figure 3B and C.
4) One of the important new claims relates to the nature of the interaction between MCU and EMRE. The authors have performed tryptophan scanning mutagenesis of both transmembranes of MCU and identify six in TM1 versus two in TM2 that disrupt function. Based on this difference (six versus two), the authors prioritize TM1 as being more important for EMRE binding. They then show that two of these mutations can be introduced into the DdMCU without impairing function – and since DdMCU operates independently of EMRE, prioritize these two as important for human MCU binding to EMRE. Finally, the authors perform an amino acid swap between MCU and EMRE to support the conclusion that A241 in MCU and F77 in EMRE directly interact.
I have some concerns about the logic and inference. First, it's not clear how the authors can exclude the possibility that the other four residues on TM1, or the other two residues on TM2, are important for EMRE binding. Second, if this model is correct, then the MCU chimera with TM1 from human, and TM2 from C. elegans, ought in principle be activated by human EMRE, yet it is not. Third, in these functional studies shown in Figure 3—figure supplement 1, IPs are not performed, so we do not know if EMRE is actually bound or not bound to MCU. The authors ought to review these data, add the necessary controls, and then state their conclusions carefully. Also, the authors cannot claim direct interaction – this is nuanced in the text, but in the figure legends, direct interaction is claimed.
5) The co-IP data presented in Figure 5E suggests that MCU can interact with MICU1 independent of EMRE, and that MICU1 can interact with EMRE independent of MCU. These experiments lack proper controls. The authors could express another mitochondrial IM protein as a negative control.
Reviewer #3 general assessment:
This is an extremely important paper that clarifies the general architecture of the MCU complex and sheds light on the physiological function of EMRE and MICU1. The experiments are carefully planned and conducted. The paper is essentially ready for publication.https://doi.org/10.7554/eLife.15545.018
- Ming-Feng Tsai
- Christopher Miller
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
We thank Dr. Vamsi Mootha for plasmids and valuable advice, Daniel Turman, Dr. Nicolas Last, Dr. Joel Meyerson, Dr. Randy Stockbridge, and Dr. Rocio Finol-Urdaneta for insightful comments of the manuscript. We are particularly grateful to Dr. Daniel Oprian for assistance for raising antibodies and producing the associated affinity media, and for supporting M.R. to participate in this project. This work is supported in part by NIH Grant R01-GM107023. The authors declare no conflict of interest.
- David E Clapham, Reviewing Editor, Howard Hughes Medical Institute, Boston Children's Hospital, United States
- Received: February 25, 2016
- Accepted: April 19, 2016
- Accepted Manuscript published: April 21, 2016 (version 1)
- Accepted Manuscript updated: May 6, 2016 (version 2)
- Version of Record published: June 3, 2016 (version 3)
- Version of Record updated: June 6, 2016 (version 4)
- Version of Record updated: March 2, 2017 (version 5)
© 2016, Tsai et al
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