1. Chromosomes and Gene Expression
  2. Genetics and Genomics
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Gene Expression: Transposons take remote control

  1. Julius Judd
  2. Cédric Feschotte  Is a corresponding author
  1. Cornell University, United States
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Cite this article as: eLife 2018;7:e40921 doi: 10.7554/eLife.40921
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Figures

Identification of genes controlled by LRT5HS elements.

Fuentes et al. used inactive Cas9 (red or green circles), guided by short RNAs (black hairpins), to deliver either repressive proteins (red crabs) to silence LTR5HS elements (left; CRISPRi), or activating proteins (green rockets) to activate LTR5HS elements (right; CRISPRa). Silencing the elements (represented by cyan boxes) repressed the expression of remote genes (white boxes), while activating the elements enhanced the expression of these genes: the level of expression associated with each gene is shown by a dark blue histogram, and the genome is represented by the black line. The Venn diagram shows that 275 genes (highlighted in pink) are both repressed by CRISPRi and activated by CRISPRa, while another 143 are repressed but not activated, and another 1886 are activated but not repressed.

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