A Bayesian analysis of the association between Leukotriene A4 Hydrolase genotype and survival in tuberculous meningitis
Abstract
Tuberculous meningitis has high mortality, linked to excessive inflammation. However, adjunctive antiinflammatory corticosteroids reduce mortality by only 30%, suggesting that inflammatory pathophysiology causes only a subset of deaths. In Vietnam, the survival benefit of antiinflammatory corticosteroids was most pronounced in patients with a C/T promoter variant in the leukotriene A_{4} hydrolase (LTA4H) gene encoding an enzyme that regulates inflammatory eicosanoids. LTA4H TT patients with increased expression had increased survival, consistent with corticosteroids benefiting individuals with hyperinflammatory responses. However, an Indonesia study did not find an LTA4H TT genotype survival benefit. Here using Bayesian methods to analyse both studies, we find that LTA4H TT genotype confers survival benefit that begins early and continues longterm in both populations. This benefit is nullified in the most severe cases with high early mortality. LTA4H genotyping together with disease severity assessment may target glucocorticoid therapy to patients most likely to benefit from it.
eLife digest
Tuberculous meningitis is a serious infection of the lining of the brain, which affects over 100,000 people a year. Without treatment, it is always fatal: even with proper antibiotics, about a quarter of patients do not survive and many will have permanent brain damage. Overactive inflammation is thought to contribute to this process. Corticosteroid drugs, which dampen the inflammatory process, are therefore often used during treatment. However, they merely reduce mortality by 30%, suggesting that only some people benefit from them.
Two recent studies have linked the genetic makeup of individuals who have tuberculous meningitis to how they respond to corticosteroids. There were, in particular, differences in the LTA4H gene that codes for an inflammationcausing protein. According to these results, only individuals carrying highinflammation versions of the LTA4H gene would benefit from the treatment. Yet a third study did not find any effect of the genetic background of patients.
All three papers used frequentist statistics to draw their conclusions, only examining the percentage of people who survived in each group. Yet, this type of analysis can miss important details. It also does not work well when the number of patients is small, or when the effectiveness of a drug varies during the course of an illness.
Another method, called Bayesian statistics, can perform better under these limitations. In particular, it takes into account the probability of an event based on prior knowledge – for instance, that the risk of dying varies smoothly with time.
Here, Whitworth et al. used Bayesian statistics to reanalyse the data from these studies, demonstrating that death rates were correlated with the type of LTA4H gene carried by patients. In particular, corticosteroid treatment worked best for people with the high inflammation versions of the gene. However, regardless of genetic background, corticosteroids were not effective if patients were extremely sick before being treated.
The work by Whitworth et al. demonstrates the importance of using Bayesian statistics to examine the effectiveness of medical treatments. It could help to design better protocols for tuberculous meningitis treatment, tailored to the genetic makeup of patients.
Introduction
Tuberculous meningitis (TBM) is the most severe form of tuberculosis. Despite effective antimicrobial therapy, it results in 20–25% mortality in HIVnegative individuals and ~40% mortality in HIVpositive individuals (Stadelman et al., 2020; Thwaites et al., 2013). A longstanding hypothesis that an excessive intracerebral inflammatory response underlies TBM mortality (Shane and Riley, 1953) led to multiple trials of adjunctive antiinflammatory treatment with corticosteroids (e.g. dexamethasone) (Prasad et al., 2016; Wilkinson et al., 2017). Findings from a randomised controlled trial (RCT) in Vietnam that adjunctive dexamethasone improved survival by ~30% led to it becoming standard of care treatment (Thwaites et al., 2004). However, the modest benefit of adjunctive dexamethasone treatment suggested a heterogeneity in glucocorticoidresponsiveness (Donald and Schoeman, 2004; Schoeman and Donald, 2013). Findings in a zebrafish model of TB provided a testable hypothesis for a mechanism underlying this heterogeneity (Thwaites et al., 2004; Tobin et al., 2012; Tobin et al., 2010). The zebrafish findings suggested that either deficiency or excess of leukotriene A4 hydrolase (LTA4H), a key enzyme that regulates the balance of pro and antiinflammatory eicosanoids, increase susceptibility to TBM for opposite reasons  too little or too much inflammation (Tobin et al., 2012; Tobin et al., 2010). It became possible to test the prediction when a common human functional LTA4H promoter variant (rs17525495) was identified comprising a C/T transition that controlled LTA4H expression, with the T allele causing increased expression (Tobin et al., 2012). A retrospective analysis of patient LTA4H rs17525495 genotypes in the Vietnam dexamethasone RCT cohort confirmed the prediction (Thwaites et al., 2004; Tobin et al., 2012). Among HIVnegative patients, the survival benefit of dexamethasone was restricted to patients with the hyperinflammatory TT genotype, with CC patients potentially harmed by this treatment (Tobin et al., 2012). These findings supported the model that mortality from TBM was due to two distinct inflammatory states, and that LTA4H genotype might be a critical determinant of inflammation and consequently of the response to adjunctive antiinflammatory treatment. If true, then personalized genotypedirected adjunctive glucocorticoid treatment would be warranted, with the drug given only to TT patients. This would be particularly important given the possible harm to the hypoinflammatory CC group, as well as the adverse effects of longterm high dose treatment with a broadly acting immunosuppressant.
To further these findings, two new studies of the association of LTA4H genotype with TBM survival in HIVnegative patients were performed in Vietnam and Indonesia, respectively (Thuong et al., 2017; van Laarhoven et al., 2017). Because glucocorticoid adjunctive therapy had become standard of care owing to the benefit observed in the randomised controlled trial (Thwaites et al., 2004), all patients received it in both studies. Therefore, the prediction that could be tested was that TT mortality is less than CC+CT mortality. Whereas the Vietnam study confirmed this prediction, the Indonesia study did not. The Vietnam cohort had an overall mortality of 18.8%, similar to that reported in the literature (Stadelman et al., 2020). A striking feature of the Indonesia cohort was its more than twofold increased mortality in comparison with the Vietnam cohort. Moreover, most of the Indonesia cohort deaths occurred early with a median time to death of eight days versus 50 days in Vietnam (Table 1). This high early mortality raised the possibility that the impact of the LTA4H variant differs by disease severity, and may not be relevant in more severe disease (Fava and Schurr, 2017). If so, then the effects of the LTA4H genotype were being masked by the preponderance of extremely severe cases in the Indonesia cohort (Fava and Schurr, 2017).
Both studies used, as the primary metric of significance testing, Cox regression modelling, an approach that assumes that the ratio of hazard rates between groups is constant throughout the observed period (Bradburn et al., 2003; Greenland et al., 2016). Therefore, this analytical method could miss important differences in these studies of TBM, a disease which can present acutely yet have a prolonged timecourse with vastly differing mortality risks over time (Thuong et al., 2017; Thwaites et al., 2013; van Laarhoven et al., 2017). Moreover, testing the hypothesis that LTA4H effects are limited to specific disease severity grades requires subgroup analysis. The use of frequentist statistics would limit the ability to perform such subgroup analyses because the penalties it sets for multiple comparisons do not reflect realworld situations (Gelman and Loken, 2013; Smith and Ebrahim, 2002). Bayesian analysis is ideally suited to simultaneously estimate treatment effects in multiple subgroups because it results in different interactions with the number of results obtained which are much less problematic than those arising in frequentist analysis (Box 1 and Appendix 1).
Box 1.
Contrast of ‘95% significant’ in Bayesian and frequentist paradigms (MacKay, 2003).
Bayes: ‘A is significantly greater than B’ = Posterior probability that A greater than B is at least 0.95.
Frequentist: ‘A is significantly greater than B’ = For any circumstance where A is at most B, the probability of getting data in this critical region, as we did, was at most 0.05.
Therefore:
We expect 1 in 20 of Bayesianly (95%) significant results to be truly negative and therefore false positives;
We expect up to 1 in 20 of truly negative results to be frequentistly significant (at the 95% level) and therefore false positives.
Therefore (assuming all positives are at 95% level):
In the frequentist paradigm, the expected number of false positive results is proportional to the number of comparisons done on true negatives;
In the Bayesian paradigm, the expected number of false positive results is proportional to the number of apparent positive results, and unaffected by any vast number of accompanying apparent negative results.
Therefore, we used a Bayesian approach to analyse data from the two cohorts (Gelman and Loken, 2013; MacKay, 2003; Zampieri et al., 2020) (Appendix 2). Bayesian analysis also enables the detection of significant differences that might be limited to just a part of the timecourse and therefore would allow analysis to be independent of the kinetics of death in the Vietnam and Indonesia cohorts. Medical management decisions are guided by an assessment of the probabilities of outcome. In TBM, the question faced by the clinician is how likely is glucocorticoid therapy going to help or harm a patient. Bayesian paradigms, unlike frequentist ones, understand probability in a realworld way, using it to indicate the plausibility of a particular conclusion (MacKay, 2003; Zampieri et al., 2020). Finally, relevant to this reanalysis of completed clinical studies, Bayesian paradigms have less potential for bias arising from posthoc analysis (Appendix 1).
The severity gradespecific analyses, coupled with temporal analyses made possible by Bayesian methods, reveal that the LTA4H TT genotype is associated with survival in both cohorts.
Results
Characteristics of the Vietnam and Indonesia cohorts
The age range of patients was similar in the Vietnam and Indonesia cohorts with Indonesia patients tending to be younger (Table 1). We compared the cohorts for disease severity on presentation using both measures used in the studies, the Glasgow Coma Score (GCS) and the modified British Medical Research Council TBM grade (TBM grade) (Box 2). The Indonesia cohort had more severe disease on presentation by both measures (Table 1 and Figure 1). We used the TBM grade for further analyses because it divides patients into just three severity groups, making comparisons more feasible. Importantly, it also provides clinically relevant separation of GCS 15 patients, the most highly represented in both cohorts (Figure 1), into those with and without focal neurological signs.
Box 2.
TBM disease severity classification.
Glasgow Coma Score (GCS) A general measure of consciousness used for a wide range of neurological deficits, particularly brain trauma, by scoring eye opening and verbal and motor responses to stimuli, to assign a numerical value from 3 to 15 corresponding to decreasing severity, where 3 corresponds to completely unresponsive, deep coma and 15 to fully conscious (Teasdale and Jennett, 1974).
Modified British Medical Research Council (BMRC) TBM Grade A classification scheme specifically tailored to assess TBM severity. It is derived from the GCS, and additionally incorporates the presence of focal neurological signs. The TBM grade is scored between 1–3 corresponding to increasing severity, converse to the GCS classification.
GCS and BMRC Grade relationship BMRC Grade 1  GCS = 15 with no focal neurological signs; BMRC Grade 2  GCS = 11–14, or GCS = 15 with focal neurological signs, BMRC Grade 3  GCS < 10 (Heemskerk et al., 2011).
LTA4H TT genotype association with survival becomes stronger with increasing disease severity in Vietnam
Because the Indonesia cohort was skewed towards more severe disease on presentation, one explanation for the lack of an LTA4H genotype association with survival in Indonesia was that the underlying association is overridden by severe disease, a strong independent correlate of mortality (Fava and Schurr, 2017; Wang et al., 2019). Indeed, a detailed comparison of the Vietnam and Indonesia cohorts showed that 76% of Indonesia patients presented in Grade 2 versus 47% of the Vietnam patients (Table 1). This increase was driven entirely by a shift from Grade 1 (9% vs 37% in Vietnam). The cohorts had nearly equal proportions of Grade 3 patients (15% each). Therefore, the ~2 foldincreased overall mortality in Indonesia could be largely accounted for by a corresponding increase of Grade 2 patients (1.6 fold higher than Vietnam). Disease severity as assessed by BMRC Grade or GCS at presentation is also a strong predictor of earlier death (Fava and Schurr, 2017; Wang et al., 2019), and the Indonesia patients died sooner (median time to death 8 days versus 50 days in Vietnam) (Table 1).
If LTA4H genotype associates with survival most strongly in mild disease, then the association seen for the entire Vietnam cohort (Thuong et al., 2017) should be strongest in Grade 1 patients. We tested this prediction with Bayesian analysis using a prior that was intentionally uninformative and very wide, while still being centred on clinicianexpected survival curves and hazard rates. We included additional parameters to allow for the possibility that not all patient deaths would be linked to the same mode of death. Importantly, the model and priors used allowed us to incorporate our preexisting knowledge that mortality risk to a population of TBM patients varies smoothly with time, rather than occurring at a number of discrete times common to all patients as is implied by the maximum likelihood solution illustrated by a KaplanMeier plot. The details of the model and the priors are in Appendix 2. The definitions of terms and abbreviations used throughout the paper are in Box 3.
Box 3.
Definitions and usages.
Definitions.
Posterior probability  the probability after seeing the data
Mean posterior survival probability at time T  the expectation after seeing the data of the fraction of patients that will still be alive at time T
Hazard rate  the fraction of those still surviving that will die per unit time. A high hazard rate at a particular time indicates that patients are at high risk of dying at that time
Mean posterior hazard rate at time T  the expectation after seeing the data of the hazard rate at time T
Abbreviated and example usages.
Onwards  for the rest of the 270 day observation period.
Throughout  for the entire 270 day observation period.
‘A was significantly greater than B at time T’  the posterior probability that A was greater than B, at time T, was at least 0.95.
‘A was not significantly different from B’  the posterior probability that A was greater than B was between 0.05 and 0.95 throughout.
‘Group A survival was 30% greater than group B’  the mean posterior survival probability at 270 days, p_{A}, was 30% absolute greater than the corresponding probability p_{B} for group B. (‘absolute’ here meaning that p_{A} = p_{B} + 0.3, and not that p_{A} = p_{B} × 1.3).
‘Probability that group A survival was better than group B at time T was 0.97’  the posterior probability that group A survival probability at time T was greater than group B survival probability at time T was 0.97 (Note that this is not a reference to the mean posterior survival probability).
'The hazard rate ratio for group A over group B peaked at X at time T and remained greater than Y throughout’ or ‘Group A had an Xfold higher relative risk of death at time T which remained greater than Y throughout’  the mean posterior hazard rate for group A, divided by that for group B, peaked at a value of X at time T and remained greater than Y at all times up to 270 days.
‘The probability that hazard rate for group A was greater than that of group B was 0.97 at time T’  the posterior probability was 0.97 that the hazard rate for group A at time T was greater than that for group B at time T (Note that this is not a reference to the mean posterior hazard rate).
‘The hazard rate for group A was greater than that for group B at time T’  the mean posterior hazard rate for group A was greater than that for group B at time T.
‘survival gap’  the difference in mean posterior survival probability between the two groups being considered at 270 days.
In the original Vietnam study, the TT genotype was associated with survival and the CC and CT genotypes had similarly increased mortality over TT (Thuong et al., 2017), so we compared TT survival to that of CT and CC combined (nonTT). We first confirmed that the TT variant distribution did not differ by grade on presentation (Table 2). Bayesian analysis confirmed that the TT genotype was associated with a survival advantage in the overall cohort (Figure 2A). Moreover, the analysis revealed that this survival advantage manifested early and persisted over most of the observation period (Figure 2A). The Bayesian method enables a more detailed evaluation of mortality risk over time through a hazard rate analysis. This analysis reinforced the significantly higher hazard rate for nonTT starting at 4 days and persisting through 120 days (Figure 2A, inset). As predicted by the earlier analysis, the survival probability between CC and CT was not significantly different at any time during the 270 day observation period (data not shown).
When we stratified the Vietnam patients by grade and LTA4H genotype, we got a surprising result. The LTA4H TT association with survival was barely present in Grade 1, a bit more in Grade 2, and strongest in Grade 3 where it reached significance (Figure 2B–D). Similar to the overall cohort, the Grade 3 increased survival probability for TT also manifested early and persisted throughout (Figure 2D). Hazard rate analysis again showed that nonTT patients had a greatly increased risk of mortality very early (Figure 2D, inset). The nonTT over TT hazard rate ratio peaked at 16 on day 3 (Figure 2D, inset). This early high peak dropped only gradually over time; it was 5 at day 100 and remained >1 throughout (data not shown). Again survival of CC and CT patients was not significantly different at any time during the 270 day observation period (data not shown).
In sum, our analysis revealed that in Vietnam, LTA4H TT was associated with survival, not in mild disease as suggested earlier (Fava and Schurr, 2017), but rather in the most severe disease grade. In fact, the bulk of the overall association was being driven by Grade 3 patients who constituted only 15.9% of the cohort (Table 1). NonTT patients were at greatest risk of dying within days of admission, a risk that diminished with time but remained greater than the TT patients throughout.
In Indonesia, the LTA4H TT genotype effect does not extend beyond Grade 2
Bayesian analysis found that, in the overall Indonesia cohort, survival of the TT patient group was higher than nonTT though falling short of significance (maximum probability 0.92) (Figure 2E). Moreover, this analysis detected that the hazard rate for nonTT patients was higher than TT patients from day 1 to day 13; the nonTT over TT ratio reached significance on days 2 and 3, at which time the nonTT hazard rate was twice that of TT. Thus, while the TT beneficial effect was weaker than in Vietnam (compare Figure 2E–A), hazard rate analysis showed that as in Vietnam, TT benefit manifested early (Figure 2E inset, compare to Figure 2A inset). For the gradestratified cohorts, the analysis of Grade 1 patients was uninformative as there was only one TT patient in this group who survived throughout (Table 2 and Figure 2F; also see Appendix 2, section 4). In Grade 2, the TT survival effect was significant, in contrast to Grade 2 Vietnam (compare Figure 2G–C). Rather, the pattern of the Grade 2 association was similar to Vietnam Grade 3 with a significant early TT survival benefit. As in Vietnam, the TT survival benefit started within days with an early hazard rate peak for the nonTT group. In Grade 3, the LTA4H TT effect was again absent (Figure 2H). As in Vietnam, survival of CC and CT patients was not significantly different at any time during the analysis for the overall cohort and for each of the three grades (data not shown).
Since Grade 2 patients constitute the bulk of the Indonesia cohort (75.5%), why was the LTA4H genotype effect in this grade not reflected in the overall cohort analysis? This was particularly curious given that in Vietnam the overall significant effect was being driven very substantially by Grade 3 patients who constituted only 15.9% of the cohort. We saw that the LTA4H TT benefit was weaker and less prolonged in Indonesia Grade 2 than in Vietnam Grade 3 (compare Figure 2G to D). NonTT patients had similar mortality in Indonesia Grade 2 and Vietnam Grade 3 (compare Figure 2G to D).
Thus, Bayesian analysis revealed an LTA4H TT survival association in Indonesia as well. The association being only in Grade 2 and not in Grade 3 patients suggested an upper limit of disease severity for its efficacy.
Gradeforgrade mortality rate differences may explain the difference in gradespecific LTA4H TT effects in Vietnam and Indonesia
Why might LTA4H effects stop after Grade 2 in Indonesia? A closer comparison of the overall survival between the two sites suggested that there were mortality differences between the two cohorts for all grades combined and in gradeforgrade comparisons that were LTA4Hindependent (Figure 2). For instance, Indonesia Grade 2 nonTT patients had a mortality risk similar to that of Vietnam Grade 3 nonTT patients (compare Figures 2D and 1G).
We confirmed by Bayesian analysis that within each cohort, mortality risk increased with grade severity (Thuong et al., 2017; van Laarhoven et al., 2017; Figure 3). From early on, Grade 1 survival was significantly greater than Grade 2, which was significantly greater than Grade 3 (Figure 3A and B). The hazard rate ratios highlighted that while the risk of increased mortality with higher grade was highest early, it was sustained longterm (Figure 3C and D). Importantly, both the survival and hazard rate analyses again pointed to an increased gradeforgrade risk of mortality in Indonesia over Vietnam. Next, we directly compared gradeforgrade mortality between the cohorts. Vietnam survival probability was higher in all grades, and significantly so for Grades 2 and 3 with survival gaps of 18% and 24%, respectively (Figure 4A,C and E). Similarly, hazard rates did not differ significantly between the cohorts in Grade 1 (Figure 4B), but there was a significant and substantial increase in early hazard rates for Indonesia Grades 2 and 3 (Figure 4D and F). We ruled out the possibility that this increased mortality was because the Indonesia patients had more severe disease within each grade as reflected in the GCS. While they had somewhat more severe disease in Grade 2, in Grade 3 where the difference was the most pronounced, they had milder disease (Figure 1).
In sum, these analyses show that the inherent higher mortality associated with more severe disease on presentation was sharply accentuated in Indonesia. Indonesia grade 2 patients experienced similar mortality risk as Vietnam Grade 3 patients with the Indonesia Grade 3 patients experiencing far greater mortality. This higher mortality could potentially explain the finding that the LTA4H TT survival advantage did not extend to Indonesia Grade 3 patients. It may be that the TT genotype advantage, in response to corticosteroid treatment, is overridden by other factors that cause extreme mortality.
Discussion
The finding in two independent cohorts in Vietnam collected from 2001 to 2004 and 2011–2015 that a common functional human variant was associated with responsiveness to adjunctive glucocorticoid treatment in TBM represented an ideal example of pharmacogenomics, coming as it did from mechanistic understanding of the underlying reason (Sadée and Dai, 2005; Thuong et al., 2017; Tobin et al., 2012). The potential importance of these findings was heightened with subsequent pathway analyses in the zebrafish (where the LTA4H link to disease severity was discovered) identifying more specific, inexpensive, steroidsparing drugs that might circumvent LTA4Hmediated pathology (Roca and Ramakrishnan, 2013; Roca et al., 2019). It was disappointing and puzzling when a similar study of TBM patients in Indonesia failed to find a significant association (van Laarhoven et al., 2017). In a commentary published alongside the Indonesia and second Vietnam studies, Fava and Schurr suggested that background genetic differences between the cohorts might account for the lack of an LTA4H association (Fava and Schurr, 2017). However, noting that the Indonesia cohort presented with more severe disease and died earlier, they postulated that rather than invoking an unknown genetic phenomenon, it was more likely that the beneficial effects of dexamethasone for the LTA4H TT genotype were nullified in more severe disease. This concept of the disease being too severe for outcomes to be influenced by intervention has precedent. The beneficial effect of fluoroquinolones in TBM is present in Grade1 or 2 disease, but lost in Grade 3 disease (Thwaites et al., 2011). To test if increased grade severity on presentation in Indonesia was sufficient to account for the loss of the LTA4H TT survival effect, a gradeforgrade analysis within and between the two cohorts was necessary, while taking into account the temporal changes in mortality risk over the several monthslong observation period. Moreover, both the magnitude and the time to mortality seemed to vary dramatically between the cohorts (Table 1). We realized that Bayesian methods were ideal for these complex and intrinsically multivariate comparisons and possibly the only path to a biologically and clinically relevant understanding.
When we analysed the Vietnam cohort separated by grade severity, we saw that there was indeed a relationship between LTA4H effect and grade severity. However, it was opposite to what had been predicted (Fava and Schurr, 2017). The TT survival benefit became stronger not weaker with increasing grade. The analysis also suggested the reason for this. Patients with mild disease on presentation did well regardless of LTA4H genotype, so that the added benefit of the TT genotype was small. In Indonesia, the LTA4H TT effect was present in Grade 2 and completely absent in Grade 3, a finding that was perplexing until we analysed the LTA4Hindependent mortality risk of the two cohorts. The Indonesia cohort did not just contain a greater number of patients presenting at a more severe grade as had been noted earlier (Fava and Schurr, 2017), but patients in this cohort had substantially higher early mortality than their Vietnam counterparts even gradeforgrade (Table 1). Indonesia patients had nearly twice the mortality risk in Grade 2 and ~50% higher risk in Grade 3. Tellingly, Grade 2 Indonesia overall mortality risk was virtually identical to Grade 3 Vietnam (38% versus 37.9%), suggesting that this level of overall mortality represents the boundary of the beneficial effect of LTA4H TT. Thus, LTA4H TT efficacy was limited by other factors that cause mortality. These factors appear independent of severity grade on presentation, and if they exceed a threshold (represented by about ~40% mortality) then the beneficial effect of LTA4H TT is lost. A limitation of this study is that we cannot definitively determine the causes of this LTA4H genotypeindependent excess Grade 3 mortality in Indonesia because the patient populations, hospital care protocols and the availability of intensive care differed between each study site. One possibility is that better ancillary care was possible in Vietnam where all patients were enrolled into a clinical trial versus only 17% in Indonesia (Thuong et al., 2017; van Laarhoven et al., 2017). The excess Grade 3 Indonesia mortality peaked within days, and optimized respiratory support, in particular, would be essential to keep patients alive through the early high risk stage in order allow for antiinflammatory effects of corticosteroids to benefit the TT patients. Thus the beneficial effect of dexamethasone to the LTA4H TT group may simply not have had time to come into play in Grade 3 Indonesia patients. A second possibility is that Indonesia Grade 3 patients presented with a greater degree of dysregulated inflammation in a manner not revealed by standard metrics of judging disease severity. If this were the case then corticosteroids might no longer be beneficial. Since both TT and nonTT patients suffered identical excess mortality risk, its cause would have been LTA4Hindependent. Indonesia patients tended to be younger than Vietnam patients in all grades (Table 1), and perhaps were more prone to develop such a response. Such a previously unrecognized form of dysregulated inflammation might be caused by other genetic variants uniquely present in the Indonesia patients and would work through an inflammatory pathway that is less responsive to dexamethasone.
Why was the LTA4H TT effect thought to be limited to the least severe patients in both cohorts (Fava and Schurr, 2017)? Two reasons might explain this. First, it was not appreciated that apart from having fewer Grade 1 patients, Indonesia patients also suffered higher gradeforgrade mortality risk (Fava and Schurr, 2017). Second, because of a paucity of less severe patients, a subgroup analysis was performed in an attempt to tease out an effect in this group. Patients were divided into GCS 14–15 (less severe) versus <13 (more severe), and a nonsignificant TT survival benefit was found in the GCS 14–15 group only, supporting the idea that TT effects, if any, were limited to the less severe group (Figure 5A and B). However, our analysis now shows the two reasons why this grouping resulted in the LTA4H TT effect going unrecognized: (1) GCS 15 does not separate patients by whether or not they have neurological signs, so it includes all BMRC Grade 1 and some BMRC Grade 2 patients (Box 2); (2) the grouping used resulted in the Grade 2 patients being split so that some were grouped with Grade 3 and the remaining with Grade 1; (3) of the 11 TT patients in the GCS 14–15 grouping only 1 TT patient was in Grade 1, with the remaining 10 in Grade 2, so the effect seen in their GCS 14–15 was entirely from Grade 2 patients (compare Figure 5B–C). In any case this subgroup TT effect would have been impossible to detect with the frequentist analyses used. The use of Bayesian methods has identified subgroups of TT patients that gain the most survival benefit from dexamethasone treatment. Our findings set the stage for using Bayesian methods for subgroup analyses of the ongoing trial with the goal of comparing the survival of CC and CT patients randomised to dexamethasone or placebo, and also comparing the survival of each of these groups individually and combined to TT patients who are all getting dexamethasone (Donovan et al., 2018; Donovan and Phu, 2020).
Finally, in addition to providing guidance for TBM pharmacogenomic approaches, we hope that our analyses highlight the unique value of Bayesian methods for providing guidance for other complex diseases with difficult treatment decisions. The vital importance of defining the patient populations and subgroups which will benefit the most from specialised interventions and treatments is increasingly appreciated (Sadée and Dai, 2005). This is not only to target such treatments to those who will benefit, but to avoid their adverse effects in those individuals who have little chance of experiencing a clinically relevant benefit from them. Our finding that the LTA4H TT genotype’s salutary role is incumbent on the optimization of other factors that maximize patient survival has broad implications for pharmacogenomic approaches.
Materials and methods
The anonymized patient cohort data used here has been previously described in detail (Thuong et al., 2017; van Laarhoven et al., 2017). Our analysis included all 439 HIVnegative patients from the Vietnam study and 376 of the 515 HIVnegative patients from the Indonesia study. The remaining 139 Indonesian patients were excluded for lack of information on LTA4H rs17525495 genotype (87), baseline disease severity (44) or outcome (8) (Supplementary file 1). All Vietnam patients were admitted to one of two tertiary care referral hospitals in Ho Chi Minh City, Vietnam: Pham Ngoc Thach Hospital for Tuberculosis and Lung Disease (designated Hospital 1) and the Hospital for Tropical Diseases (designated Hospital 2) (Heemskerk et al., 2016). All patients were treated with adjunctive dexamethasone for the first 6–8 weeks with the regimen adjusted to disease severity on presentation (Thwaites et al., 2004).
Patient cohorts were compared overall as well as stratified into disease severity groups based on the TBM grade and by LTA4H genotypes, into the TT group (previously linked to response to steroids) and the CC, CT, and combined CC and CT genotypes (nonTT group). The Bayesian analysis methods to compare survival probabilities are detailed in Appendix 2. We limited analysis to the first 9 months of the oneyear observation period in Indonesia to be compatible with the 9 month observation period in Vietnam. For the data in Tables 1 and 2, Bayesian posterior probabilities for the comparisons of genotype and GCS frequencies, for each bin separately and for the mean of the distribution, were based on a flat Dirichlet prior on the bin probabilities.
Comparisons of age and time to median mortality were done using comparisons of arithmetic means of distributions, allowing Bayesian model choice between the following distribution families: Gaussian, logGaussian, Student, logStudent, Gamma, inverseGamma, Gammapower. For age, logGaussian had overall the highest posterior probability; for time to median mortality, inverseGamma was preferred.
Appendix 1
Comparison of Bayesian and frequentist methods
1. Introduction
We give here a brief comparison of Bayesian methods with the more commonly encountered frequentist methods familiar to all scientists.
For context we consider a situation where we have collected some data $x$ (potentially a vector of many variables) involving two sets of patients A and B, and want to determine whether an underlying parameter $a$ of set A is greater or smaller than the corresponding parameter $b$ of set B; these form our two hypotheses H_{0} (that $a\le b$) and H_{1} (that $a>b$).
We describe first the primary characteristics of each method, and then consider the consequences for the further properties they display.
2. The Bayesian method
The Bayesian method answers the question:
What is the probability that $a>b$ given the data ?
giving answers of the form
The probability given the data that $a>b$ is 0.96.
or (with the same meaning)
The posterior probability that $a>b$ is 0.96.
In order to answer this question, the Bayesian method requires as input (a) the data $x$ and (b) probability distributions on each of the unknown parameters which describe what was known about their values before collecting the data. The latter are known as the "prior(s)". This is in keeping with reality — what we know after collecting some data depends not only on the data but also on what we knew before collecting the data. The answer is then calculated using Bayes’ theorem.
Note that it is not possible to say ‘We know nothing about the unknown parameters’, and indeed this is never the case (e.g. if the unknown parameter is probability of survival at one year, then we know it is between 0 and 1, and unlikely to be either 0 or 1 exactly). However, one can almost always (and in particular in the present context) give distributions which leave wide open the range of values the parameters could take.
As a result, a Bayesian analysis, in addition to reporting the results, also reports the priors used, and what difference changing the priors makes to the results — in this case see sections 2.3 and 5 of Appendix 2 for these points. If priors are chosen to permit a broad range of parameter values (as in the present paper), it is usually the case that changing them to any other such prior has little effect on the results, so long as there is a reasonable quantity of data.
3. The frequentist method
The frequentist method aims to (a) avoid having to consider what was known before collecting the data, and (b) to control the probability of deciding H_{1} is true if in fact H_{0} is true (the ‘type I error rate’). To achieve this aim it answers a more complicated question; we give a version of it specific to a particular level of frequentist ‘confidence’:
Did the data $x$ fall into a critical region C, which we chose before collecting any of the data, such that, for any set of values $h$ of the unknown parameters for which $a\le b$, the probability given $h$ of $x$ falling into C is at most 0.04 ?
If the answer is Yes, this fact is usually encoded in a statement such as
We are 96% (frequentist)confident that $a>b$ by the onesided Student ttest.
or just
$a>b$ ($p=0.04$, onesided Student ttest).
Here the $p$ value is usually one minus the frequentist confidence, and ‘onesided Student ttest’ specifies a particular standard set of nested critical regions (one for each level of confidence). For all but the simplest of frequentist tests, the user only rarely attempts to visualise or imagine the nested family of critical regions, which are often/usually multidimensional, as would be true in the present case were we to use frequentist methods.
In doing this the frequentist method treats H_{0} differently from H_{1}, and thereby replaces the Bayesian’s prior with an asymmetry on the hypotheses that doesn’t correspond to any prior.
4. Consequent differences in behaviour
The frequentist’s avoidance of using a prior and emphasis on controlling the type I error rate together incur a significant cost.
First, it is an absolute requirement of the frequentist method that the critical region be specified before the data is collected. However, this requirement is very often ignored, and this can lead to bias in a posthoc analysis, for example if the investigator first tries a MannWhitney Utest, finds the result not significant, so tries a Student ttest, finds it significant, and so only reports the latter. Where the unknowns are more than 1dimensional, as in the present survival analyses, this ambiguity gets exponentially worse with the number of dimensions.
The Bayesian method has no such restriction: a data set may be analysed multiple times posthoc with different priors either to investigate the effect of changing the prior or because different workers have different prior beliefs.
However, both methods can potentially be biased by omitting to report findings which are not to the investigator’s liking. In this paper, the only important omission is that analysis was also done of the HIVpositive patients in Vietnam, and this was not reported in this paper. This was because, although it tended to confirm similar findings to those from the HIVnegative cohort, there were some points that were less clearcut and harder to interpret, and its publication therefore awaits completion of a clinical trial in which the benefit of dexamethasone is being examined for HIVpositive patients of all three genotypes by randomizing them to get dexamethasone or not (Donovan et al., 2018).
Second, repeated analyses at different stages of data collection and/or analyses of multiple subsets of the patients posthoc increase the type I error rate, and therefore incur penalties in the frequentist method, making it more difficult for any individual comparison to reach significance. In contrast the Bayesian method controls the posterior probability (which is unaffected by other independent analyses being done), and not the type I error rate. The effects of this difference are summarised in Box 1 in the main paper.
Third, frequentist analysis fails to respect commonsense realworld laws of inference. For example, it is often the case that a frequentist analysis will find that it is e.g. 95%confident that $a$ is nonzero, but 97.5%confident that $a>0$, a stronger statement than that $a\ne 0$. In contrast Bayesian methods, being rigorously derived from probability theory, obey the laws that probability already obeys.
Fourth, frequentist analysis gives priority to controlling the type I error rate (i.e. the probability that it concludes H_{1} holds if in fact H_{0} holds), and almost never controls the probability that it concludes that H_{0} holds if in fact H_{1} holds. (Some frequentist analyses do report ‘power calculations’, which calculate the probability that it concludes that H_{0} holds if in fact some subset of H_{1} holds, e.g. the part of H_{1} where $a>b+10$ rather than that just $a>b$.) In contrast Bayesian methods treat H_{0} and H_{1} identically.
Appendix 2
Supplementary methods
1. Overview of methods for survival analysis
We adopt the Bayesian paradigm. (For those who haven’t encountered Bayesian methods before, a comparison with frequentist hypothesis testing is given in Appendix 1.) Accordingly we define below a probabilistic generative model for patient lifetime $x$ given model parameters $\theta$ and a suitable prior distribution on $\theta$. We assume that, when observed, such lifetime data may be censored at known times $t$ that are independent of the underlying lifetimes and the model parameters, so that for each patient we observe either a time of death $x$ or a time of censoring $t$ at which the patient was alive.
We then collect a data set of values $\widehat{\mathbf{\mathbf{x}}}$ of $x$ or $t$ for a set of patients, and apply Bayes’ theorem to deduce the posterior distribution $P(\theta \widehat{\mathbf{\mathbf{x}}})$ of the model parameters.
Since this distribution is hard to visualise, we draw samples of $\theta$ from it using Markov chain Monte Carlo (MCMC) methods described below. For each such sample of $\theta$ we calculate the distribution of lifetime $x$ given $\theta$ and hence the survival probability $q(t\theta )$ and hazard rate $h(t\theta )$ functions against patient time since admission, using the following relationships:
An example resulting set of survival probability curves is shown in Appendix 2—figure 1, with the corresponding hazard rate curves being shown in Appendix 2—figure 2.
Now, given a set of such curves, we can calculate the mean posterior survival probability (resp. hazard rate) at each time point and plot the posterior mean survival probability (resp. hazard rate) against time. Similarly we can find the 2.5% and 97.5% centiles. Examples of the posterior mean and centile curves for survival probability corresponding to Appendix 2—figure 1 are shown in Appendix 2—figure 3.
Further, given two such sets of survival probability or hazard rate curves (such as the survival probability curves shown in Appendix 2—figure 4 and zoomed in in Appendix 2—figure 5), we can also calculate at each time point the probability that a value taken at that time from a random curve from set 1 is greater than a similar value taken from a random curve from set 2. As we can see from these two figures, this probability is higher at 10 days than at 1 day or 100 days. These probabilities, at each time point, can then be plotted giving in this example Appendix 2—figure 6, showing the probability that underlying survival in group 1 is greater than that in group 2 at each time point.
In order to carry out this process, we need to specifically define the lifetime distribution model $P(x\theta )$, and we now turn to this.
2. Lifetime model
We suppose that there exist an unknown number $j$ of different mechanisms causing death, and that each such mode of death has a different lifetime distribution.
2.1 Combination of different modes of death
Let $x$ denote a lifetime, that is, the time until a patient dies. Let $j\in \{1,2,\mathrm{\dots},J\}$ denote a particular mechanism (or mode) of death. Let $x}_{j$ denote the time at which mode $j$ would kill the patient; we set ${x}_{j}=\mathrm{\infty}$ to denote the possibility that that mode would never have killed the patient.
Then the patient’s time of death is given by
In particular $x=\mathrm{\infty}$ denotes the situation that the patient never dies (unlikely as this is).
2.2 Model of a single mode of death
We now drop the subscripts $j$, but assume that this subsection will be repeated $J$ times with the subscript $j$s added to every random variable, with each of the repetitions being independent as far as the model is concerned before being conditioned on observed data. When later we want to refer to the complete set of $J$ values of e.g. $p$, we will use bold face, e.g. $\mathbf{\mathbf{p}}=({p}_{1},{p}_{2},\mathrm{\dots},{p}_{J})$.
Thus we will set $P(xp,k,m,r)$, i.e. $P({x}_{j}{p}_{j},{k}_{j},{m}_{j},{r}_{j})$, to be such that with probability $p$, ${x}^{k}$ is Gamma distributed with parameters ${m}^{\prime}=m$ and ${r}^{\prime}=m{r}^{k}$, and otherwise $x=\mathrm{\infty}$. Thus we have
Here $p\in [0,1]$, $k\in \mathbb{R}\setminus \{0\}$, $m,r>0$.
Note that we have here a distribution which has both a discrete and a continuous part, so that $P(x\mathrm{\dots})$ is used as notation both for a probability and for a probability density: in other words, we have a continuous distribution for finite positive $x$, given by a density function, whose interpretation is that its integral from $x}_{1$ to $x}_{2$ is the probability that ${x}_{1}<x<{x}_{2}$; but as we have a nonzero probability $1p$ that $x=\mathrm{\infty}$, the integral from 0 (inclusive) to $\mathrm{\infty}$ (exclusive) of the density given by the first line of the above formula for $P(xp,k,m,r)$ must be $p$. On the other hand we have a discrete distribution for $x=\mathrm{\infty}$, and $1p$ is a probability, not a density.
By way of very approximate intuition: $p$ is the probability that a particular mode of death would kill the patient at a finite time; $r$ is the reciprocal of the overall timescale to deaths of those patients who die; $m$ governs how variable those times of death are – the smaller $m$ is, the more variable are the times of death; and the sign of $k$ plays a part in determining whether the hazard rate for this mode of death is increasing or decreasing, while the magnitude of $k$ governs how abruptly the spread of death time is cut off in the less spread out direction. Specifically, $k=0$ makes no sense, as then we would have ${x}^{k}=1$ for all $x$, and an invalid distribution would result (so it should not be a surprise that the prior on $k$ is bimodal with zero density at zero).
2.3 Priors on the parameters
We specify the priors on the parameters in two stages. First, we specify their general form, and second we choose specific values for the hyperparameters that then specify a unique prior.
2.3.1 General form of the priors
The total number $j$ of modes is itself to be considered a random variable, on which we put the prior
for $J\in {\mathbb{N}}^{*}=\{1,2,\mathrm{\dots}\}$ and for some fixed ${\alpha}_{\text{J}}\in [0,1).$
The prior for the parameters $p,m,r,k$ of each mode of death are taken to be independent, and as follows.
We take the prior on $p$ to be Beta, with positive real parameters ${\alpha}_{\text{p}},{\beta}_{\text{p}}>0$, so that
We take the prior on r to be Gamma, with parameters ${m}_{\text{r}},{r}_{\text{r}}>0$, so that
We take the prior on each of the parameters $k$ and $m$ to be the conjugate prior on each with respect to this parameterisation. Thus for positive real parameters ${a}_{\text{m}},{b}_{\text{m}}$ we have
Similarly for parameters ${N}_{\text{k}}\in \mathbb{N}$, ${a}_{\text{k}}\in {\mathbb{R}}_{+}$ and ${\mathbf{\mathbf{b}}}_{\text{k}}=({b}_{1},{b}_{2},\mathrm{\dots},{b}_{{N}_{\text{k}}}),{\mathbf{\mathbf{c}}}_{\text{k}}=({c}_{1},{c}_{2},\mathrm{\dots},{c}_{{N}_{\text{k}}})\in {\mathbb{R}}_{+}^{{N}_{\text{k}}}$ we have
2.3.2 Specific values of the hyperparameters and the resulting priors
Specific values were chosen for the hyperparameters by varying them and showing those users overseeing the analysis (namely LW, MT, PE, LR, of whom PE and LR are infectious diseases clinicians) the resulting distributions of samples of survival curves and hazard rates, then letting them choose the most appropriate prior given their background experience. The prior chosen was intentionally uninformative and very wide, while still being centred on the clinicianexpected survival curves and hazard rates.
The specific parameter values chosen were as follows: ${a}_{\text{J}}=0.8$
These result in the following depicted distributions for $J$, $p}_{j$, $m}_{j$, $r}_{j$, $k}_{j$, and hence for the depicted samples from the distributions for survival probability and hazard rate against time as well as the mean and 2.5% and 97.5% centiles for the last two: see Appendix 2—figures 7 to 15.
2.4 Summary
Thus the parameters $\theta$ consist of $\theta =(J,\mathbf{\mathbf{p}},\mathbf{\mathbf{m}},\mathbf{\mathbf{r}},\mathbf{\mathbf{k}})$, and the prior
a combination of densities of different dimensionalities. This can be regarded a mixture of models, one for each possible value of $J$, weighted by the prior on $J$: the model for $J=1$ has only one possible mode of death, while that for $J=2$ has two possible modes of death, which are independent, and so on for higher values of $J$.
In particular, when comparing two subsets A and B of patients, we use the same prior to infer the posterior distribution of $\theta$ given $\widehat{\mathbf{\mathbf{x}}}$ for each subset of the patients independently. Thus a priori the probability at each time $t$ that ${q}_{\text{A}}(t)>{q}_{\text{B}}(t)$ is $\frac{1}{2}$, the probability that ${q}_{\text{A}}(t)<{q}_{\text{B}}(t)$ is $\frac{1}{2}$, and the probability that ${q}_{\text{A}}(t)={q}_{\text{B}}(t)$ is zero (though it has nonzero probability density). In other words we are asking how sure we are that ${q}_{\text{A}}(t)>{q}_{\text{B}}(t)$ given the data, reckoning the alternative to be that ${q}_{\text{A}}(t)<{q}_{\text{B}}(t)$, and taking for granted that the probability that the two are exactly the same is zero.
2.5 Rationale
The basic Gamma model is a frequently used model of a failure mode that goes through several stages of failing, each with an exponentially distributed lifetime, before final failure occurs. The additional effect of the parameter $k$ is to incorporate Weibulltype failure time models, allowing for both increasing and decreasing hazard rates. The parameter $p$ allows for the possibility that some modes of dying may not be relevant for all patients, while the combination of the $J$ submodels allows for a number of different types of mechanisms of death to be relevant.
In particular, we specifically used independent priors on the parameters of the subsets being compared because:
When offered the choice the users (namely LW, MT, PE, LR, GT of whom PE, LR, and GT are infectious diseases clinicians with GT having extensive experience with TB meningitis patients) indicated unanimously that this correctly represented their prior beliefs;
Because we restrict our use of posterior probabilities to those of the form $P(U>V\text{data})$ rather than $P(U>V+\alpha \text{data})$ for some $\alpha >0$, and because the additional effect of any nonindependent factors in the prior would be symmetric either side of the difference between the two subsets being zero, the effect of any nonindependent factors would be expected to be very small;
If they did not believe the priors on the relevant disjoint subsets were independent, the clinicians involved would find it very hard to specify exactly how similar each pair of subsets being compared should be expected to be.
3 MCMC methodology
We introduce additional variables $j}_{i$ for each patient $i$ which indicate whether the time of death was censored (value 0) or was caused by a particular mode $j$ of death (value $j\ne 0$ unknown). We also introduce variables ${x}_{i,j}$ of unknown values giving for each patient the time of death that would have resulted from mode $j$ if no other modes had killed the patient first. These variables take the specific value ${x}_{i,j}=\mathrm{\infty}$ if mode $j$ would in fact not have killed patient i at any finite time.
We initialise the parameters $J,\mathbf{\mathbf{p}},\mathbf{\mathbf{m}},\mathbf{\mathbf{r}},\mathbf{\mathbf{k}}$ from the prior and initialise the additional variables $\mathbf{\mathbf{j}}$ and $\mathbf{\mathbf{x}}$ randomly to any set compatible with those and the observed variables $\widehat{\mathbf{\mathbf{x}}}$. These variables then form ${\theta}_{1}=(J,\mathbf{\mathbf{p}},\mathbf{\mathbf{m}},\mathbf{\mathbf{r}},\mathbf{\mathbf{k}},\mathbf{\mathbf{j}},\mathbf{\mathbf{x}})$, the first of a sequence of samples ${({\theta}_{n})}_{n=1,\mathrm{\dots}}$ to be drawn.
3.1 Sampling methods
A thorough review of all the following methods is available either in Neal, 1993 or in Dagpunar, 1988 except where otherwise indicated.
The key point is that if we resample each variable by a method that satisfies detailed balance, and given other weak conditions which are here fulfilled, Feller’s theorem (Neal, 1993) then guarantees the sequence of samples $({\theta}_{n})$ will eventually converge to a sequence of samples from the desired distribution $P(\theta \widehat{\mathbf{\mathbf{x}}})$. The samples in this sequence will not be independent of each other, though the conditional distribution of ${\theta}_{{n}_{1}}$ given ${\theta}_{{n}_{0}}$ will also converge to $P(\theta \widehat{\mathbf{\mathbf{x}}})$ as ${n}_{1}\to \mathrm{\infty}$ with $n}_{0$ fixed, that is, to independence.
Sampling from the posterior was done by the MCMC technique of Gibbs sampling, that is, sampling from the following distributions palindromically:
$P(\mathbf{\mathbf{k}}\widehat{\mathbf{\mathbf{x}}},\mathbf{\mathbf{x}},J,\mathbf{\mathbf{j}},\mathbf{\mathbf{m}},\mathbf{\mathbf{r}},\mathbf{\mathbf{p}})$. This distribution has two parts (${k}_{j}>0$ and ${k}_{j}<0$), each of which is logconcave. We therefore first resample the sign of each $k}_{j$ using the MetropolisHastings algorithm (Neal, 1993), then use adaptive rejection sampling (Gilks, 2019) to resample the magnitude of $k}_{j$ given its sign, then resample the sign again to maintain detailed balance.
$P(\mathbf{\mathbf{p}}\widehat{\mathbf{\mathbf{x}}},\mathbf{\mathbf{x}},J,\mathbf{\mathbf{j}},\mathbf{\mathbf{m}},\mathbf{\mathbf{r}},\mathbf{\mathbf{k}})$. In this case the conditional distribution is from the Beta family, and standard methods (Dagpunar, 1988) are available to sample from it.
$P(\mathbf{\mathbf{m}}\widehat{\mathbf{\mathbf{x}}},\mathbf{\mathbf{x}},J,\mathbf{\mathbf{j}},\mathbf{\mathbf{r}},\mathbf{\mathbf{k}},\mathbf{\mathbf{p}})$. This distribution is log concave, so we may use adaptive rejection (Gilks, 2019) sampling to sample from it.
$P(\mathbf{\mathbf{r}}\widehat{\mathbf{\mathbf{x}}},\mathbf{\mathbf{x}},J,\mathbf{\mathbf{j}},\mathbf{\mathbf{m}},\mathbf{\mathbf{k}},\mathbf{\mathbf{p}})$. For each $j$, this distribution is in general a product of a Gamma distribution on $r}_{j$ and a much narrower Gamma distribution on ${r}_{j}^{{k}_{j}}$. We therefore sample from the Gamma relevant to the latter (Dagpunar, 1988), using this as a proposal distribution for the MetropolisHastings algorithm (Neal, 1993), resulting in the Hastings ratio coming from the Gamma on r_{j}.
$P(\mathbf{\mathbf{j}}\widehat{\mathbf{\mathbf{x}}},J,\mathbf{\mathbf{m}},\mathbf{\mathbf{r}},\mathbf{\mathbf{k}},\mathbf{\mathbf{p}})$ then $P(\mathbf{\mathbf{x}}\widehat{\mathbf{\mathbf{x}}},J,\mathbf{\mathbf{j}},\mathbf{\mathbf{m}},\mathbf{\mathbf{r}},\mathbf{\mathbf{k}},\mathbf{\mathbf{p}})$. The first of these is a discrete distribution which is trivial to sample from, and the second reduces to a truncated Gamma distribution. To sample from the latter we divide into two cases: if the shape parameter is $\ge 1$ the distribution is logconcave and we can use adaptive rejection sampling (Gilks, 2019) otherwise we use MetropolisHastings (Neal, 1993) with either an exponential or a Gamma proposal distribution, depending which is estimated to be likely to be quicker given the other parameters.
$P(J\widehat{\mathbf{\mathbf{x}}},\mathbf{\mathbf{x}},\mathbf{\mathbf{j}},\mathbf{\mathbf{m}},\mathbf{\mathbf{r}},\mathbf{\mathbf{k}},\mathbf{\mathbf{p}})$ (where only values of $j$ unused in $\mathbf{\mathbf{j}}$ are allowed to be removed) followed, if $J$ has increased, by sampling the new elements of $\mathbf{\mathbf{m}},\mathbf{\mathbf{r}},\mathbf{\mathbf{k}},\mathbf{\mathbf{p}}$ from the prior distributions on these variables. Resampling of $J$ uses a discrete conditional distribution, and is done using a proposal to either increase or decrease $J$ by 1, and applying the appropriate Hastings ratio (Neal, 1993) to reject the proposal in such a way as to achieve detailed balance.
10,000 samples were drawn from the posterior for each subset of the data considered (e.g. for Indonesian TT patients). The first 1500 samples were discarded and the remainder kept for analysis. To check that the software was correct we undertook two types of check:
The inference code was reviewed by somebody (RFS) different from its author (JC) looking for bugs, and those found were removed after RFS and JC had conferred to reach agreement on them.
Multiple sets of synthetic data were generated (for which the true values of $\mathbf{\mathbf{x}},J,\mathbf{\mathbf{j}},\mathbf{\mathbf{m}},\mathbf{\mathbf{r}},\mathbf{\mathbf{k}},\mathbf{\mathbf{p}}$ were therefore known) and the posterior distributions were compared with the true values (as for example in Appendix 2—figure 1 above).
3.2 Convergence checks
In addition we checked for convergence of the Markov chains by starting them from different random initial values of $\mathbf{\mathbf{x}},J,\mathbf{\mathbf{j}},\mathbf{\mathbf{m}},\mathbf{\mathbf{r}},\mathbf{\mathbf{k}},\mathbf{\mathbf{p}}$, then the two corresponding sets of output samples were compared as if they were made from two different sets of patients, giving plots analogous to Appendix 2—figures 4 and 6 above, such as those shown in Figures 16 and 17 below, which show that the two distributions are sufficiently close as to be in practice indistinguishable.
4. Example of inference
Because, in the Results section of this paper, one particular specific example of Bayesian inference occurs whose interpretation is slightly tricky, it seems appropriate to discuss it specifically here. This corresponds precisely to the comparison of TT and nonTT genotypes in Grade 1 Indonesia patients.
We refer to Appendix 2—figure 18. The situation before knowledge of the data is described by the prior mean survival probability curve in solid magenta and its 2.5% and 97.5% centiles in dotdash magenta, constructing the 95% prior confidence interval. (You may think, in the light of the data, that this prior is too pessimistic — but the point is that this is what was thought before knowledge of the data.) Note that the only parts of the plot outside this confidence interval are a small piece at the bottom left and an extremely thin sliver along the righthand part of the top edge.
We now collect the TT patients’ data: there is, however, only 1 TT patient, who survives until 1 year before being censored. A single patient, however, has only a small effect on the prior (just as a single headtoss would not convince you a coin was biased): this shifts the posterior for the TT group upwards to the red lines, mean (solid) and centiles (dotdash).
On the other hand when we collect the nonTT patients’ data, there are 33 of them, so they have a bigger effect, both raising the mean and narrowing the 95% posterior confidence interval to the corresponding green plots. Even though these 33 patients survive less well than the single TT patient, they lift the posterior mean more than does the single TT patient, but the green 95% posterior confidence interval is much narrower than the red one.
Finally, analogous to Appendix 2—figures 4 and 6, we can calculate the probability that the TT population survives better than the nonTT population at each time point, getting Appendix 2—figure 19: the conclusion is that it has become very slightly less probable that TT survives better than nonTT than it was before (before it was 0.5 precisely), but in essence the posterior probability that TT survives better than nonTT remains not far off 0.5 throughout the timecourse.
Of course, in most examples in the paper, there are more patients in both groups being compared, and we are more likely to get a more definite conclusion.
5. Sensitivity to choice of priors
Specifically for comparisons of TT and nonTT subsets, where the subsets consist of very different numbers of patients, there is particular scope for otherwise unexpected sensitivity to the choice of uninformative priors. To assess this we initially checked, for one such comparison, the effect of using a different prior, namely:
which results in the set of samples of survival probability curves shown in Appendix 2—figure 20.
As can be seen by comparing this with Appendix 2—figure 12, this alternative prior does not envisage nearly as many early deaths in the first few days as the chosen prior, but equally believes it to be more unlikely that survival would be near 100% at much later times.
The effect of this on a comparison of a small subset and a large subset would be expected to be to shift the posterior on the small subset upwards in the early period and downwards in the late period compared with the large subset, increasing the significance of the early comparison if the small subset survived better than the large subset at that time, and reducing it if in the other direction (and vice versa at late times).
In the case of the comparison shown in Appendix 2—figure 6 above, switching to the alternative prior gives Appendix 2—figure 21, indeed confirming this expectation: the peak significance, at around 10 days, increases from 0.994 to 0.996, while at 1 year the comparison probability reduces from 0.893 to 0.886 .
We remark, however, that even with this significant change in the prior, the inferred comparison probabilities change remarkably little. We have therefore not reported detailed comparisons of alternative priors throughout the results.
Data availability
Excel spreadsheets with all patient data are included.
References

Survival analysis part II: multivariate data analysisan introduction to concepts and methodsBritish Journal of Cancer 89:431–436.https://doi.org/10.1038/sj.bjc.6601119

Tuberculous meningitisNew England Journal of Medicine 351:1719–1720.https://doi.org/10.1056/NEJMp048227

Adjunctive dexamethasone for the treatment of HIVuninfected adults with tuberculous meningitis stratified by leukotriene A4 hydrolase genotype (LAST ACT): Study protocol for a randomised double blind placebo controlled noninferiority trialWellcome Open Research 3:32.https://doi.org/10.12688/wellcomeopenres.14007.1

Evaluating the impact of LTA4H genotype and immune status on survival from tuberculous meningitisThe Journal of Infectious Diseases 215:1011–1013.https://doi.org/10.1093/infdis/jix052

ReportThe Garden of Forking Paths: Why Multiple Comparisons Can Be a Problem, Even When There Is No “Fishing Expedition” or “PHacking” and the Research Hypothesis Was Posited Ahead of TimeDepartment of Statistics, Columbia University.

Statistical tests, P values, confidence intervals, and power: a guide to misinterpretationsEuropean Journal of Epidemiology 31:337–350.https://doi.org/10.1007/s1065401601493

Intensified antituberculosis therapy in adults with tuberculous meningitisNew England Journal of Medicine 374:124–134.https://doi.org/10.1056/NEJMoa1507062

Bayesian Inference and Sampling Theory456–466, Information Theory Inference and Learning Algorithms, Bayesian Inference and Sampling Theory, Cambridge University Press.

ReportProbabilistic Inference Using Markov Chain Monte Carlo Methods, Technical Report CRGTR931, Department of Computer ScienceUniversity of Toronto.

Corticosteroids for managing tuberculous meningitisCochrane Database of Systematic Reviews 4:CD002244.https://doi.org/10.1002/14651858.CD002244.pub4

Pharmacogenetics/genomics and personalized medicineHuman Molecular Genetics 14 Spec No. 2:R207–R214.https://doi.org/10.1093/hmg/ddi261

BookTuberculous meningitisIn: Singh O. D, editors. Handbook of Clinical Neurology. Elsevier. pp. 1135–1138.https://doi.org/10.1016/B9780444529107.000337

Tuberculous meningitis: combined therapy with cortisone and antimicrobial agentsThe New England Journal of Medicine 249:829–834.https://doi.org/10.1056/NEJM195311192492101

Treatment outcomes in adult tuberculous meningitis: a systematic review and MetaanalysisOpen Forum Infectious Diseases 7:ofaa257.https://doi.org/10.1093/ofid/ofaa257

Leukotriene A4 hydrolase genotype and HIV infection influence intracerebral inflammation and survival from tuberculous meningitisThe Journal of Infectious Diseases 215:1020–1028.https://doi.org/10.1093/infdis/jix050

Dexamethasone for the treatment of tuberculous meningitis in adolescents and adultsNew England Journal of Medicine 351:1741–1751.https://doi.org/10.1056/NEJMoa040573

Randomized pharmacokinetic and pharmacodynamic comparison of fluoroquinolones for tuberculous meningitisAntimicrobial Agents and Chemotherapy 55:3244–3253.https://doi.org/10.1128/AAC.0006411

Tuberculous meningitis: more questions, still too few answersThe Lancet Neurology 12:999–1010.https://doi.org/10.1016/S14744422(13)701686

Tuberculous meningitisNature Reviews Neurology 13:581–598.https://doi.org/10.1038/nrneurol.2017.120

Effects of a resuscitation strategy targeting peripheral perfusion status versus serum lactate levels among patients with septic shock. A bayesian reanalysis of the ANDROMEDASHOCK trialAmerican Journal of Respiratory and Critical Care Medicine 201:423–429.https://doi.org/10.1164/rccm.2019050968OC
Decision letter

Bavesh D KanaSenior and Reviewing Editor; University of the Witwatersrand, South Africa
In the interests of transparency, eLife publishes the most substantive revision requests and the accompanying author responses.
Acceptance summary:
Your description of the potential role of the LTA4H TT variant of Leukotriene A4 Hydrolase in mediating outcomes of tuberculosis meningitis has important implications for developing precision medicine approaches for this and other related inflammatory diseases. The use of Bayesian analysis in your study allowed for a detailed investigation of the contribution of this genotypic variant and has also pointed to other factors that may mediate treatment and disease outcomes, thus creating potential new avenues of research.
Decision letter after peer review:
Thank you for submitting your article "A Bayesian analysis of the association between Leukotriene A4 Hydrolase genotype and survival in tuberculous meningitis" for consideration by eLife. Your article has been reviewed by Bavesh Kana as the Senior Editor, a Reviewing Editor, and three reviewers. The reviewers have opted to remain anonymous.
The reviewers have discussed the reviews with one another and the Reviewing Editor has drafted this decision to help you prepare a revised submission.
As the editors have judged that your manuscript is of interest, but as described below that additional analyses are required before it is published, we would like to draw your attention to changes in our revision policy that we have made in response to COVID19 (https://elifesciences.org/articles/57162). First, because many researchers have temporarily lost access to the labs, we will give authors as much time as they need to submit revised manuscripts. We are also offering, if you choose, to post the manuscript to bioRxiv (if it is not already there) along with this decision letter and a formal designation that the manuscript is "in revision at eLife". Please let us know if you would like to pursue this option. (If your work is more suitable for medRxiv, you will need to post the preprint yourself, as the mechanisms for us to do so are still in development.)
Summary:
Two Tuberculosis meningitis (TBM) studies conducted in Vietnam and Indonesia to assess the role of polymorphisms in the leukotriene A4 hydrolase (LTA4H) gene, implicated in modulating inflammation, yielded divergent conclusions. The submission by Whitman and colleagues utilizes a Bayesian approach to further investigate the data associated with these studies in an attempt to reconcile the observations. The underlying premise is that the Indonesian cohort was skewed towards more severe disease at presentation, which nullified the effect of the LTA4H genotype with patient survival in the Indonesian cohort.
1) By using a Bayesian approach in the analysis of both previous studies the authors are able to reconcile, in part, the divergent findings of an impact of LTA4H polymorphisms on TBM survival.
2) An unexpected finding was the protective effect of the LTA4H TT genotype on TBM response to glucocorticoid therapy in Vietnamese grade 3 cases. In contrast, in the Indonesian study the impact was found for grade 2 but not grade 3 patients.
3) The lack of effect by the TT genotype in the Indonesian Grade 3 TBM patients could be, as shown in the discussion, due to individuals who are genetically hyper susceptible to uncontrolled inflammation independently of LTA4H, differences in treatment and/or access to topquality care.
The authors propose that LTA4H genotyping together with data on disease severity could be used to identify TB meningitis patients most likely to benefit from adjunctive glucocorticoid treatment. This is an important study and could shed light on the discordant results obtained from the studies conducted in Vietnam and Indonesia.
Essential revisions:
1) The authors state that the higher mortality in Indonesia was driven by a higher percentage of patients with Grade 2 disease (% with Grade 3 disease being equal in the two countries). They state that the LTA4H TT genotype effect does not extend beyond Grade 2 in Indonesia. In Vietnam though, the effect of TT is most pronounced in Grade 3. In people living with HIV, the TT effect was present in people with Grade 1 or 3 disease, but not with Grade 2 disease. The latter is dismissed as “likely spurious”. However, another explanation is that disparate results in different countries, different stages of disease, and different populations suggests that LTA4H is less useful than other factors (other genetics, environment, etc) in determining disease severity and possibility of treatment response with steroids. Can the authors provide a convincing argument otherwise?
2) Further to the point above, the MRC grade is generally thought to have good association with outcomes and thus, the similar mortality for Indonesia Grade 2 nonTT patients and Vietnam Grade 3 nonTT patients clearly suggests that grade and LTA4H genotyping may not always be able to identify TB meningitis patients most likely to benefit from adjunctive glucocorticoid treatment. Therefore, this conclusion should be toned down in the abstract and the manuscript. Perhaps LTA4H genotyping and mortality risk for each MRC grade (local to that setting) is most correlative of who is most likely to benefit from adjunctive glucocorticoid treatment? Importantly, it is still not clear why a gradespecific mortality difference was noted between the two cohorts. Was it related to the difference in critical / hospital care for these cohorts? Or other aspects? The authors speculate this but do not provide sufficient data, please address this.
3) In Indonesia, the average time to death was 8 days, and in Vietnam it was 50 days. The authors describe LTA4H TT effect as occurring over short or long periods of time, so this adds the element of longitudinal assessment and resulting effect to the mix. What effect would LTA4H play late in disease, once a patient is already on multidrug therapy as well as steroids, from a mechanistic point of view?
4) Was any analysis performed to test whether the patients removed from the cohorts did not skew the population? That is, were the demographic characteristics of the patients excluded from this analysis similar to those included in the study?
5) Provide p values for comparisons across the three subpopulations in Table 1 and Table 2 as well as Supplementary file 1?
6) While substantial details are presented in supplementary methods, a brief introduction (in simple language) on Bayesian methods would be useful for the reader to better understand the main methodology utilized in this study. For example, the statement in subsection “LTA4H TT genotype association with survival becomes stronger with increasing disease severity in Vietnam HIVnegative patients”, "Importantly, the model and priors used allowed us to incorporate our preexisting knowledge that mortality risk to a population of TBM patients varies smoothly with time, rather than occurring at a number of discrete times common to all patients as is implied by the maximum likelihood solution illustrated by a KaplanMeier plot." helps understand the basis for the use of Bayesian analysis.
7) In most clinical trials, assumptions, sample size calculations and outcome measures are predefined to limit bias related to posthoc analysis. Can the authors present some information on how bias was prevented? If this was not feasible, this should be listed as a study limitation.
8) These data are insightful, but as state above, it is still possible that another genotype or local characteristic of the population (Vietnam versus Indonesia) is / are confounding the results. For example, one of the coauthors of the current study has demonstrated that cerebral tryptophan metabolism (under strong genetic influence) is important for the outcome of TB meningitis (Lancet Infect Dis. 2018). Can the authors analyse the contribution other genetic factors based on currently available genotypic information from these cohorts or from other studies?
9) The authors suggest that “LTA4H genotyping together with disease severity assessment may target glucocorticoid therapy to patients most likely to benefit from it,” but they provide no road map for operationalising the finding. In whom should this genotyping be done? What role does the test result have in decisions regarding provision of steroids? Please provide a decision framework or a tool for use of this test plus data that a clinician would have.
[Editors' note: further revisions were suggested prior to acceptance, as described below.]
Thank you for resubmitting your work entitled "A Bayesian analysis of the association between Leukotriene A4 Hydrolase genotype and survival in tuberculous meningitis" for further consideration by eLife. Your revised article has been evaluated by Bavesh Kana (Senior Editor) and a Reviewing Editor.
The manuscript has been improved but there are some remaining issues that need to be addressed before acceptance, as outlined below:
Reviewers have accepted all revisions and explanations but request the following modifications:
You reword your conclusions to discuss additional limitations. For example, the statement, "Thus, LTA4H TT efficacy was limited by other factors that cause mortality. These factors appear independent of severity grade on presentation, and if they exceed a threshold (represented by about ~ 40% mortality) then the beneficial effect of LTA4H TT is lost." This is largely speculative and lacking in detail. What are these proposed factors? Carefully couching the conclusions in context of all limitations will enhance the value of your work and appeal to a broader audience who may be interested in these additional factors.
https://doi.org/10.7554/eLife.61722.sa1Author response
Essential revisions:
1) The authors state that the higher mortality in Indonesia was driven by a higher percentage of patients with Grade 2 disease (% with Grade 3 disease being equal in the two countries). They state that the LTA4H TT genotype effect does not extend beyond Grade 2 in Indonesia. In Vietnam though, the effect of TT is most pronounced in Grade 3. In people living with HIV, the TT effect was present in people with Grade 1 or 3 disease, but not with Grade 2 disease. The latter is dismissed as “likely spurious”. However, another explanation is that disparate results in different countries, different stages of disease, and different populations suggests that LTA4H is less useful than other factors (other genetics, environment, etc) in determining disease severity and possibility of treatment response with steroids. Can the authors provide a convincing argument otherwise?
First, we have decided to remove the HIV cohort data from the paper so as to not distract from the core finding of the paper, namely that the use of Bayesian methods has enabled us to see that the LTA4H TT genotype does improve survival in the context of dexamethasone in Indonesia also, and that this effect is affected by mortality driven by other factors. An ongoing dexamethasone trial in HIVpositive patients who will be assessed for LTA4H genotype and other detailed parameters should resolve the question of whether HIVpositive individuals fare better with dexamethasone and if so, whether the TT genotype is relevant to their dexamethasoneresponsiveness (Donovan et al., (2018).). We will use these analyses in that paper. The analytical methods and computational programs developed here will be invaluable in analyzing the results from that trial.
Second, we have no data on whether the LTA4H TT genotype determines disease severity and have not claimed that it does.
Finally, we respectfully differ from the reviewers’ suggestion that LTA4H is less useful than other factors in determining treatment response with steroids. Rather our careful and detailed analysis strongly suggests that the TT genotype does play a substantial and likely dominant role in steroid responsiveness, unless the patient is so sick upon entry with such advanced pathophysiology that they cannot be rescued by dexamethasone.
2) Further to the point above, the MRC grade is generally thought to have good association with outcomes and thus, the similar mortality for Indonesia Grade 2 nonTT patients and Vietnam Grade 3 nonTT patients clearly suggests that grade and LTA4H genotyping may not always be able to identify TB meningitis patients most likely to benefit from adjunctive glucocorticoid treatment. Therefore, this conclusion should be toned down in the abstract and the manuscript. Perhaps LTA4H genotyping and mortality risk for each MRC grade (local to that setting) is most correlative of who is most likely to benefit from adjunctive glucocorticoid treatment? Importantly, it is still not clear why a gradespecific mortality difference was noted between the two cohorts. Was it related to the difference in critical / hospital care for these cohorts? Or other aspects? The authors speculate this but do not provide sufficient data, please address this.
We are puzzled by the reviewers’ reasoning that “… the similar mortality for Indonesia Grade 2 nonTT patients and Vietnam Grade 3 nonTT patients clearly suggests that grade and LTA4H genotyping may not always be able to identify TB meningitis patients most likely to benefit from adjunctive glucocorticoid treatment.” In both cohorts, there is a clear association between MRC grade and survival with the Indonesia cohort faring worse at every grade. This finding came as a surprise to all of us because we had all thought that the difference in overall survival between the cohorts was simply because Indonesia had more severe Grade patients. This surprising result first became apparent when we analysed the TT versus nonTT data in Figure 2. To validate this, we performed the Gradespecific survival analyses in Figure 3 and then the direct headon comparison of gradespecific survival in the two cohorts in Figure 4. If we take all these results together, the most parsimonious conclusion is that the TT genotype provides a benefit up to a point of no return, i.e. up to Grade 2 in Indonesia. After that it cannot help, for the reasons described in the paper and reiterated in the following paragraph. It is not surprising therefore, that in the absence of the TT genotype, Indonesia Grade 2 patients have a similar mortality to Grade 3 Vietnam patients, just as they do in the overall cohort (Figure 3).
In response to the reviewers’ further queries, we have ruled out that there was a hidden increased severity in Grade 3 Indonesia as reflected by the GCS scores which are sensitive for smaller changes in severity in Grades 2 and 3. While Grade 2 disease in Indonesia was somewhat more severe in Vietnam, Grade 3 disease was less severe. These new analyses are presented in Supplementary file 1 and in the Discussion.
Furthermore, we don’t think that we have downplayed the genotypeindependent mortality factors. Rather, we have discussed them as extensively as we can in the Discussion. The Abstract also explicitly specifies that genotypebased therapy has its limitations: “However, its benefit is nullified in the most severe cases where other factors cause early mortality.” Based on our understanding of the levels of care available in Indonesia and Vietnam our suspicion is that some or most of these differences are attributable to the ability to provide expert intensive respiratory and other critical care support at the study site hospitals. We know that all patients in Vietnam were cared for in hospitals with critical care expertise whereas in Indonesia this was only possible for a small fraction of the cohort. However, this is a hypothesis that can only be addressed by patientchart level review and by determination of the mortality rates for other critical illnesses at the same participating hospitals. We do not have those data, and thus have to leave this as an unproven hypothesis. Therefore, all that we can say is what is already in the Discussion: “The more likely possibility is that better ancillary care was possible in Vietnam where all patients were enrolled into a clinical trial versus only 17% in Indonesia (Thuong et al., 2017; van Laarhoven et al., 2017). Optimized respiratory support, in particular, would be essential to keep patients alive through the early highrisk stage in order allow for antiinflammatory effects of corticosteroids to benefit the TT patients.”
3) In Indonesia, the average time to death was 8 days, and in Vietnam it was 50 days. The authors describe LTA4H TT effect as occurring over short or long periods of time, so this adds the element of longitudinal assessment and resulting effect to the mix. What effect would LTA4H play late in disease, once a patient is already on multidrug therapy as well as steroids, from a mechanistic point of view?
As shown in Figure 2, the LTA4H TT survival benefit starts early, with hazard rate ratios peaking in the range of 17 days. The finding that the biggest effect of dexamethasone on this genotype occurs early is not surprising given its role in countering inflammation. The reviewers are right – the effect wanes only gradually and in most cases does not vanish for several months or even through the analysis period. This is reflected in the TTnonTT survival gap gradually increasing through this period in almost all cases. We think that this is more likely because of a knockon benefit of the early reduction of acute inflammation by steroids rather than a different effect of the TT genotype in distinct pathophysiology operant only later. The former model would be more consistent with our understanding of TBM pathogenesis.
4) Was any analysis performed to test whether the patients removed from the cohorts did not skew the population? That is, were the demographic characteristics of the patients excluded from this analysis similar to those included in the study?
This information is now included in Supplementary file 1. We have decided that a formal analysis is not appropriate given that the reason that the patients were excluded is because they had key information missing. We knew the ages of all the patients who were excluded and the Bayesian comparison probability of the mean of the excluded distribution being greater than the included on was not significant (P 0.85, inverseΓ preferred distribution).
5) Provide p values for comparisons across the three subpopulations in Table 1 and Table 2 as well as Supplementary file 1?
Bayesian comparison probabilities have now been computed for these comparisons for each line of Table 1 and Table 2 and described in the Materials and methods. The original Supplementary file 1, which pertained to the HIVpositive patients has been removed. (The new Supplementary file 1 contains the information described in the point 4 response above.)
6) While substantial details are presented in supplementary methods, a brief introduction (in simple language) on Bayesian methods would be useful for the reader to better understand the main methodology utilized in this study. For example, the statement in subsection “LTA4H TT genotype association with survival becomes stronger with increasing disease severity in Vietnam HIVnegative patients”, "Importantly, the model and priors used allowed us to incorporate our preexisting knowledge that mortality risk to a population of TBM patients varies smoothly with time, rather than occurring at a number of discrete times common to all patients as is implied by the maximum likelihood solution illustrated by a KaplanMeier plot." helps understand the basis for the use of Bayesian analysis.
Thank you for encouraging us to describe this methodology further. We have added this information in Appendix 1, comparing frequentist and Bayesian paradigms and refer to it at the end of the paragraph discussing Bayesian analysis (Introduction).
7) In most clinical trials, assumptions, sample size calculations and outcome measures are predefined to limit bias related to posthoc analysis. Can the authors present some information on how bias was prevented? If this was not feasible, this should be listed as a study limitation.
A major strength of Bayesian analysis is that bias due to posthoc subgroup analysis doesn't arise, unless one only provides a selected subset of the results that suit one’s thesis. In response to the reviewers’ comment, we have included in the Introduction the following sentence:
“Finally, relevant to this reanalysis of completed clinical studies, Bayesian paradigms have less potential for bias arising from posthoc analysis (Appendix 2).” We have also included details of this aspect of Bayesian analysis in Appendix 2 section 1.3. Since, we have now decided to withhold the HIVpositive data for the time being, we note that in this section, we have mentioned in it that we left out the HIVpositive data with the following lines:
“In this paper, the only important omission is that analysis was also done of the
HIVpositive patients in Vietnam, and this was not reported in this paper. This was because, although it tended to confirm similar findings to those from the HIVnegative cohort, there were some points that were less clear cut and harder to interpret, and its publication therefore awaits completion of a clinical trial in which the benefit of dexamethasone is being examined for HIVpositive patients of all three genotypes by randomizing them to get dexamethasone or not.”
8) These data are insightful, but as state above, it is still possible that another genotype or local characteristic of the population (Vietnam versus Indonesia) is / are confounding the results. For example, one of the coauthors of the current study has demonstrated that cerebral tryptophan metabolism (under strong genetic influence) is important for the outcome of TB meningitis (Lancet Infect Dis. 2018 May;18(5):526535). Can the authors analyse the contribution other genetic factors based on currently available genotypic information from these cohorts or from other studies?
This is a good idea but beyond the scope of this study which was focused on the Indonesia LTA4H conundrum. It is our hope that the powerful analytical methods that we have developed here will be used by the community, and we are of course providing the code and methods with a commitment to help answer questions as possible.
9) The authors suggest that “LTA4H genotyping together with disease severity assessment may target glucocorticoid therapy to patients most likely to benefit from it,” but they provide no road map for operationalising the finding. In whom should this genotyping be done? What role does the test result have in decisions regarding provision of steroids? Please provide a decision framework or a tool for use of this test plus data that a clinician would have.
This is an interesting idea. Upon reflection, however, we think it is premature to suggest a decision algorithm until further information becomes available. In particular, we await the results of the ongoing trial randomizing CC and CT patients to dexamethasone or placebo. As with the HIVpositive patients, he analysis methods and computational programs developed for this study will be invaluable in analyzing the results of this trial.
[Editors' note: further revisions were suggested prior to acceptance, as described below.]
The manuscript has been improved but there are some remaining issues that need to be addressed before acceptance, as outlined below:
Reviewers have accepted all revisions and explanations but request the following modifications:
You reword your conclusions to discuss additional limitations. For example, the statement, "Thus, LTA4H TT efficacy was limited by other factors that cause mortality. These factors appear independent of severity grade on presentation, and if they exceed a threshold (represented by about ~ 40% mortality) then the beneficial effect of LTA4H TT is lost." This is largely speculative and lacking in detail. What are these proposed factors? Carefully couching the conclusions in context of all limitations will enhance the value of your work and appeal to a broader audience who may be interested in these additional factors.
We thank the reviewers for their careful review and are very pleased that they have approved our revisions.
We have made the following changes in response to their one outstanding request (Discussion section):
1) We have explicitly stated that we do not know for certain what the cause of excess Grade 3 LTA4H deaths in Indonesia is.
2) We have more clearly divided up the possible causes into nongenetic, ancillary care related causes and LTA4Hindependent other genetic causes.
We hope this meets with the reviewers’ approval. The changes are here for the reviewers’ convenience:
“Thus the beneficial effect of dexamethasone to the LTA4H TT group may simply not have had time to come into play in Grade 3 Indonesia patients. A second possibility is that Indonesia Grade 3 patients presented with a greater degree of dysregulated inflammation in a manner not revealed by standard metrics of judging disease severity. If this were the case then corticosteroids might no longer be beneficial. Since both TT and nonTT patients suffered identical excess mortality risk, its cause would have been LTA4Hindependent. Indonesia patients tended to be younger than Vietnam patients in all grades (Table 1), and perhaps were more prone to develop such a response. Such a previously unrecognized form of dysregulated inflammation might be caused by other genetic variants uniquely present in the Indonesia patients and would work through an inflammatory pathway that is less responsive to dexamethasone.”
https://doi.org/10.7554/eLife.61722.sa2Article and author information
Author details
Funding
National Institutes of Health (NIAID ULTIMATE project 1R01AI14578101)
 Arjan van Laarhoven
 Reinout van Crevel
Wellcome Trust (Wellcome International Intermediate Fellowship 206724/Z/17/Z)
 Nguyen Thuy Thuong Thuong
Wellcome Trust (110179/Z/15/Z)
 Guy E Thwaites
Wellcome Trust (Principal Research Fellowship 103950/Z/14)
 Lalita Ramakrishnan
National Institutes of Health (R37 AI054503)
 Lalita Ramakrishnan
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Acknowledgements
We thank R Troll for evaluating the two published cohort studies, realizing that Bayesian analysis could provide answers and initiating the collaboration with the Bayesian statistician RS.
Senior and Reviewing Editor
 Bavesh D Kana, University of the Witwatersrand, South Africa
Publication history
 Received: August 3, 2020
 Accepted: November 22, 2020
 Version of Record published: January 8, 2021 (version 1)
 Version of Record updated: January 15, 2021 (version 2)
Copyright
© 2021, Whitworth et al.
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
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