Alexander S Hauser, Charlotte Avet ... David E Gloriam
The GPCR-G protein coupling map and selectivity insights will catalyze advances in receptor research, cellular signaling, and drug discovery exploiting G protein signaling bias to design safer drugs.
Development, validation, and use of an effector membrane translocation biosensor platform reveals G protein coupling selectivity signatures for 100 GPCRs that range from remarkable selectivity to full promiscuity toward the different G protein subtypes.
New open-source method contains all the tools for extracting open chromatin or RNA from thousands of single cells in parallel for <5% of the cost of state-of-the-art commercial alternatives, paving the way for whole-tissue atlasing and new protocol development.
The proposed method deciphers which low-level patterns (such as spikes) control high-level features (behavior) in relative small biological datasets, taking into account the statistical dependencies between such patterns.
Lisa L Kirkemo, Susanna K Elledge ... James A Wells
Enzyme tethering of promiscuous biotinylators to the cell surface allows for more rapid and efficient comparative surface proteomic analysis of cells and extracellular vesicles.
Giorgio Oliviero, Sergey Kovalchuk ... Ole N Jensen
Quantitative proteomics reveals the diversity and heterogeneity of nuclear proteomes in mammalian organs and identifies the molecular features of ageing.
Korin Sahinyan, Darren M Blackburn ... Vahab D Soleimani
Single myofiber ATAC-Seq provides assessment of chromatin accessibility of a single myofiber without the confounding effects of other cell types present in skeletal muscle.
Ten-fold Robust Expansion Microscopy (TREx) is a new method for 10-fold physical expansion of specimens in one round of gel embedding, enabling nanoscale imaging of antibody stains and ultrastructural detail.
