Using single nucleus RNA sequencing, a complete catalogue of cell types was determined for the mouse iris, and this information was used as a starting point to define the effects of pupil dilation on gene expression and on nuclear morphology.
Methods for screening hundreds of DNA sequences for enhancer activity have been extended for use in vivo, uncovering novel enhancer elements active in the early postnatal brain.
A unique toolbox of novel mouse lines expressing optical effectors and sensors in cardiovascular, smooth muscle, and additional lineages allows extensive in vivo and ex vivo analysis of complex biological systems.
Tamara Garrido-Gomez, Nerea Castillo-Marco ... Carlos Simon
The in vivo transcriptomic fingerprint encoding defective decidualization comprises 120 genes associated with an imbalance hormonal signaling in the decidual endometria of women who developed severe preeclampsia.
Patrick C Hoffmann, Stefano L Giandomenico ... Wanda Kukulski
Combining cerebral organoid technology with cryo-correlative microscopy reveals the organization of cytoskeleton, membrane compartments, and protein synthesis machinery contributing to the rapid expansion of developing human axons.
Fluorescence fluctuation spectroscopy is implemented for detection of up to four molecular species, allowing users to quantify molecular interactions and stoichiometry of multicomponent complexes in live cells, in a wide range of biological processes, from membrane signaling to viral assembly.
