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    1. Neuroscience

    Cross-species analysis defines the conservation of anatomically segregated VMH neuron populations

    Alison H Affinati, Paul V Sabatini ... Alan C Rupp
    This atlas and analysis of conserved ventromedial hypothalamic nucleus (VMH) neuron populations provides an unbiased starting point for the analysis of VMH circuitry and function.
    1. Neuroscience

    Metal microdrive and head cap system for silicon probe recovery in freely moving rodent

    Mihály Vöröslakos, Peter C Petersen ... György Buzsáki
    A reusable system of 3D printed stainless steel microdrive and plastic head cap allows stable recording of electrophysiological data in mice and rats and easy recovery of silicon probes.
    1. Neuroscience

    Optogenetic strategies for high-efficiency all-optical interrogation using blue-light-sensitive opsins

    Angelo Forli, Matteo Pisoni ... Tommaso Fellin
    A new soma-targeted variant of the large-conductance blue-light-sensitive opsin CoChR combined with advanced optical stimulation methods allows high-efficiency all-optical neuronal imaging and stimulation in mouse brain in vivo.
    1. Developmental Biology

    Live-imaging of endothelial Erk activity reveals dynamic and sequential signalling events during regenerative angiogenesis

    Kazuhide S Okuda, Mikaela S Keyser ... Benjamin M Hogan
    Live-imaging of a transgenic reporter of vascular Erk signalling in zebrafish reveals sequential signalling mechanisms during the initiation of regeneration in wounded blood vessels.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    A subcellular map of the human kinome

    Haitao Zhang, Xiaolei Cao ... Bin Zhao
    An immunofluorescent analysis of 465 protein kinases revealed a substantial role of liquid-liquid phase separation in kinase localization and identified new kinase localizations to mitochondria and other organelles.
    1. Neuroscience

    EEG-based detection of the locus of auditory attention with convolutional neural networks

    Servaas Vandecappelle, Lucas Deckers ... Tom Francart
    Convolutional neural networks can decode whether a person is listening to a speaker on the left or right solely from 1 to 2s of EEG data.
    1. Cell Biology
    2. Computational and Systems Biology

    Artistoo, a library to build, share, and explore simulations of cells and tissues in the web browser

    Inge MN Wortel, Johannes Textor
    By letting users build interactive simulations directly in a web browser, Artistoo unlocks new ways to communicate, teach, and collaborate in computational biology research.
    1. Genetics and Genomics
    2. Neuroscience

    Thirst interneurons that promote water seeking and limit feeding behavior in Drosophila

    Dan Landayan, Brian P Wang ... Fred W Wolf
    Thirsty seeking interneurons in Drosophila differentially regulate water intake and feeding behavior.
    1. Neuroscience

    Standardized and reproducible measurement of decision-making in mice

    The International Brain Laboratory, Valeria Aguillon-Rodriguez ... Anthony M Zador
    A standard for complex mouse behavior that can be successfully reproduced across laboratories, along with open-access data and tools to implement provide a resource for reproducibility through collaborative open-science approaches.
    1. Neuroscience

    Permeabilization-free en bloc immunohistochemistry for correlative microscopy

    Kara A Fulton, Kevin L Briggman
    Immunohistochemical labeling of antigens deep within mouse brain tissue sections was achieved without the need for lipid permeabilization, thereby preserving tissue ultrastructure and enabling correlative light and electron microscopy studies.