Bruno Bockorny, Lakshmi Muthuswamy ... Senthil K Muthuswamy
Proteomic profiling of extracellular vesicle proteins from patients with benign and malignant pancreatic diseases offers a valuable resource for identifying biomarkers for pancreatic cancer detection.
A transcriptomic atlas of the mouse vomeronasal organ reveals novel receptor co-expression patterns and provides a framework for understanding sensory neuron lineage and receptor specification.
The spatial autocorrelation method Local Moran’s I was used for image segmentation to delineate pixel clusters representing congruous objects with fuzzy borders, in this case potassium ion channel clusters.
A single-nucleus atlas of bone repair uncovers the trajectories of periosteal skeletal stem/progenitor cells in response to fracture and identifies injury-induced fibrogenic cells as intermediate osteochondroprogenitors and key paracrine regulators.
While monitoring neuronal activity in freely moving Drosophila is challenging, HI-FISH enables brain-wide mapping of active neurons and reveals behavior-specific neural activity.
A deep learning-based system objectively and reliably scores the Rey–Osterrieth complex figure test, enhancing the assessment of visual memory deficits from hand-drawn images in clinical settings.
John H Day, Catherine M Della Santina ... Laurie A Boyer
A simple and accessible method for high-throughput super-resolution fluorescence microscopy that is compatible with commonly used staining practices, 96-well cell culture plates, and confocal microscopes.
The automated workflow significantly enhances the precision and scalability of cell cycle analysis, enabling high-throughput studies in both non-adherent and adherent cells for advanced cancer research and drug development.
Mighten C Yip, Mercedes M Gonzalez ... Craig R Forest
Patch-walking is a novel automated patch clamp approach for finding synaptic connections in brain tissue, yielding 80–92% more probed connections than traditional approaches.
A novel pair of isobaric crosslinking reagents is described, which allow relative quantification of crosslinker-modified peptides during mass spectrometry analysis for comparative structural studies of proteins and protein complexes.
