Most of the DNA in a cell is tightly wrapped around groups of proteins called histones, which gives the impression of beads on a string. These bead-like structures are called nucleosomes, and interactions between histones in different nucleosomes can link one nucleosome to another, to package the DNA into a very condensed form.

Viruses sometimes interact with this condensed DNA; for example, a virus called human cytomegalovirus is known to attach to condensed DNA when cells are preparing to divide. But the consequences of these interactions are not always clear.

Now, Fang, Chen et al. have worked out the three-dimensional structure of a protein from the cytomegalovirus while it is attached to a nucleosome. This structure revealed that the viral protein connects to same part of the histones that otherwise helps pull the nucleosomes together.

Further experiments then compared how the cytomegalovirus protein attaches to nucleosomes with the interaction between nucleosomes and a similar protein from a different virus. Both viral proteins were seen to interact with the same part of the histone protein, but in different ways.

Next, Fang, Chen et al. showed that the DNA is more loosely packed when the cytomegalovirus protein is attached to the nucleosomes. This was not the case for the similar protein from the other virus. The experiments show that small differences in the ways viral proteins interact with condensed DNA can change their effects on DNA packaging. Additionally, these findings may help scientists to better understand how the binding of the cytomegalovirus protein to the nucleosomes might affect this virus’s ability to infect or cause illness in humans.

In eukaryotes, nuclear DNA is highly packaged into chromatin by histones. The nucleosome, the basic repeating unit of chromatin, typically assembles 146–147 bp of DNA wrapped around a histone octamer consisting of two copies each of H3, H4, H2A and H2B (Luger et al., 1997a). Nucleosomes connected by linker DNA form a 10-nm array resembling “beads-on-a-string”. The binding of linker histone further compacts the linear array into a more condensed 30-nm chromatin fiber. The interaction between the N-terminal tail of histone H4 and a specific surface region on the neighboring nucleosome termed the“acidic patch” plays crucial roles for the formation of 30-nm chromatin fiber (Dorigo et al., 2004; Luger et al., 1997a; Song et al., 2014). The acidic patch is formed by a number of negatively charged residues of H2A and H2B, including Glu56, Glu61, Glu64, Asp90, Glu91 and Glu92 of H2A, and Glu102 and Glu110 of H2B (Luger et al., 1997a). Several nucleosome-binding proteins have been shown to specifically interact with the acidic patch. Therefore, it has been hypothesized that these proteins may play a role in regulating the higher-order chromatin structure by competing with the H4 N-terminal tails for binding to the acidic patch of the nucleosome (Kalashnikova et al., 2013; McGinty and Tan, 2015).

The 72-kDa immediately early 1 (IE1) protein of human cytomegalovirus (hCMV) plays critical roles in the viral early gene expression and DNA replication (Gawn and Greaves, 2002; Mocarski et al., 1996). In addition, IE1 has been long known to associate with condensed host chromatin during mitosis (Ahn et al., 1998; Dimitropoulou et al., 2010; Huh et al., 2008; Krauss et al., 2009; Lafemina et al., 1989; Nevels et al., 2004; Reinhardt et al., 2005; Shin et al., 2012; Wilkinson et al., 1998). The chromatin-tethering domain (CTD) located at the very C-terminal end of IE1 (a.a 476–491) is responsible for the association with the mitotic chromosome, specifically through the acidic patch of the nucleosome (Mucke et al., 2014; Reinhardt et al., 2005). However, the molecular mechanism underlying the association and the impact on the structure and function of host chromatin by IE1 remain to be determined. Here we provide an analysis of the structural basis for the interaction between the CTD of IE1 (IE1-CTD) and the nucleosome core particle (NCP) and explore the impact of their binding on the higher-order structure of chromatin.

The complex of NCP with an IE1-CTD peptide (a.a. 476–491) was obtained by soaking the peptide into preformed NCP crystals, and a 2.8 Å structure was solved by molecular replacement. The structure shows one molecule of IE1-CTD bound to the NCP (Figure 1A; Figure 1—figure supplement 1). The presence of only one IE1-CTD peptide in the complex structure is due to the availability of only one side of the NCP surface in the preformed crystal lattice, as in the case of NCP in complex with the latency-associated antigen (LANA) of Kaposi’s sarcoma-associated herpes virus (KSHV) (Barbera et al., 2006). The IE1-CTD peptide adopts an extended, v-shaped conformation with a short α-helix at its C-terminus. In the complex structure, IE1-CTD is well positioned into the acidic patch of the nucleosome formed by H2A and H2B (Figure 1A and B). IE1-CTD contacts histone H2B at two distinct sites, the C-terminal portion of α1 and the N-terminal half of αC, through van der Waals interaction and intermolecular hydrogen bonds via its mainchain groups. Specifically, the amide and carbonyl groups of Thr480 make hydrogen bonds with the mainchain carbonyl and the sidechain amide groups of Gln44 of H2B (amino acid residue numbering following that in the reference of Luger et al., 1997a); the amide and carbonyl groups of Val484 bond the carboxylate group of Glu110 and the nitrogen atom of the imidazole ring of His106, respectively (Figure 1B). IE1-CTD contacts histone H2A via a number of sidechain contacts. His481 makes one hydrogen bond with Glu56 of H2A; Thr485 and Ser487 each makes one hydrogen bond with Glu64 of H2A; and Arg486 makes hydrogen bonds with Glu61 on α2, as well as with Asp90 and Glu92 located on αC of histone H2A. In addition, Met483 of IE1-CTD is placed in a hydrophobic pocket consisting of Leu23, and the aliphatic portion of Glu56 and Tyr57.

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The nucleosomal acidic patch is well known for hosting the binding of a number of proteins (Armache et al., 2011; Arnaudo et al., 2013; Barbera et al., 2006; Kato et al., 2013; Makde et al., 2010; McGinty et al., 2014; Wang et al., 2013; Yang et al., 2013). Most closely related to hCMV IE1 is LANA of KSHV (Barbera et al., 2006). The N-terminal CTD of LANA forms a hairpin-like structure that pokes into the acidic patch of the nucleosome (Figure 1C). The distinctly folded IE1-CTD and LANA-CTD share certain common features of nucleosome binding. Structural comparison reveals that three IE1-CTD residues, Met483, Arg486 and Ser487, occupy the same regions of the acidic patch and engage the same sets of histone residues as the corresponding LANA residues in binding the nucleosome (Figure 1C, D, 2A and B). In particular, Arg486 interacts with the acidic patch in a manner commonly found in the structures of protein-nucleosome complexes known to date (Figure 2A–F). A careful examination of the acidic patch reveals that it can be divided into three adjoining ligand-binding zones (Figure 2C). Zone I is formed by Glu61, Leu65, Asp90 and Glu92 of histone H2A, and Glu102 and Leu103 of histone H2B; Zone II is formed by histone H2A residues Tyr57, Ala60, Glu61 and Glu64, and the latter serves as a ridge separating zone I and II; and zone III is formed by Glu56 and Ala60 of H2A, and Val41, Gln44 and Glu110 of histone H2B. The binding of an arginine in zone I is conserved among all protein-NCP complexes known thus far. Thr485 of IE1 and Leu8 of LANA are bound in zone II, which is unoccupied in other NCP complexes (Figure 2). Main differences accounting for the specific interaction between IE1-CTD and NCP appear to reside in His481, which is bound in zone III and makes a hydrogen bond with Glu56 of H2A, and Thr485, which is bound to zone II and forms a hydrogen bond with Glu64 of H2A. And finally, the binding of the N-terminal segment of IE1-CTD spanning residues 476–480 to α1 of H2B is unique to IE1 (Figure 1B).

Figure 2

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To reveal the determinants for the binding specificity of IE1-CTD, we carried out structure-guided mutagenesis of IE1-CTD and histones and analyzed their interactions using isothermal titration calorimetry (ITC). Wild-type IE1-CTD bound to recombinant human NCP with a dissociation constant (K_D) of 0.4 μM, while its mutant lacking the N-terminal segment interacting with α1 of H2B (Δ476–480) reduced the nucleosome-binding affinity to a K_D of 12 μM (Figure 3A and B). To put the binding affinities in perspective, an approximately five-fold weaker binding of full-length IE1 than IE1-CTD alone to NCP was observed (Figure 3—figure supplement 1A and B), possibly caused by self-inhibitory effects of other domains in the full-length protein. In comparison, IE1 lacking the CTD showed no detectable binding to NCP (Figure 3—figure supplement 1C). With IE1-CTD, the most severe reduction of binding affinity was seen in the H481A mutant, which has a K_D of 43.4 μM (Figure 3C), while loss of one hydrogen bond by substituting Thr485 with a valine brought the K_D to 11.3 μM (Figure 3D). To gain further insights into the ~hundred-fold reduction of binding affinity introduced by the H481A mutation, we measured the effect of histone H2A mutation on Glu56, which interacts with His481 via hydrogen bonding and Met483 by hydrophobic/van der Waals interaction. ITC measurement shows that the NCP reconstituted with the E56R mutant of H2A, a charge-swap mutant, lowered the binding to IE1-CTD to a level beyond detection (Figure 3E). This change of histone H2A also brought about a conspicuous reduction of the binding of LANA to NCP from a K_D of 0.25 to 23.4 μM (Figure 3F and G). LANA and IE1 share the methionine-mediated interaction with Glu56 in zone III of the acidic patch (Figure 2A and B), and the greater compromise of the binding of IE1-CTD to the H2A-E56R NCP reflects the importance of the His481-Glu56 hydrogen bond in the IE1-NCP complex, as compared to the van der Waals interaction between Thr14 of LANA and Glu56 of histone H2A (Figure 1B).

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The acidic patch of the nucleosome has been implicated in mediating higher-order chromatin folding via interaction with the N-terminal tail of histone H4 (Luger et al., 1997a; Schalch et al., 2005). Our previous cryo-EM structure of 30-nm chromatin fiber reveals that N-terminal tails of histone H4 are involved in inter-nucleosomal contacts between the tetranucleosomal structural units through the acidic patches of adjacent nucleosomes (Song et al., 2014). Since IE1-CTD is bound at the acidic patch, it is conceivable that IE1 binding may interfere with proper folding of the 30-nm chromatin fiber. To determine the extent by which the folding of chromatin fiber is affected by IE1 binding, we incubated IE1-CTD with the in vitro reconstituted 30-nm chromatin fiber assembled with an array of 12 tandem nucleosomes carrying repeats of 177 bp 601 DNA in the presence of linker histone H1, and analyzed the sample by analytical ultracentrifugation in sedimentation velocity (AUC) (Song et al., 2014). The nucleosomal arrays used for reconstituting 30-nm chromatin fiber were highly saturated and homogeneous, as examined by micrococcal nuclease (MNase) digestion and electron microscopy (Figure 4—figure supplement 1A and B). For comparison, the same batch of nucleosomal array (without H1) and 30-nm chromatin fiber (with H1) were used in AUC analysis. AUC experiments showed that, in the absence of IE1-CTD, the nucleosomal array sedimented with a median sedimentation coefficient S_ave (sedimentation coefficient at 50% boundary fraction) of 36 ± 1 S, and the 30-nm chromatin fiber sedimented at 51.5 ± 0.6 S (Figure 3A). In the presence of IE1-CTD, the 10-nm nucleosomal array was unaffected while S_ave of the 30-nm chromatin fiber shifted from 51.5 to 48 S, indicating that the binding of IE1-CTD made the chromatin fiber more loosely folded (Figure 4A). It should be emphasized that the IE1-CTD-containing chromatin fiber represents an altered chromatin state different from both the extended 10-nm nucleosomal array and the folded 30-nm chromatin fiber. This chromatin-alteration property of IE1-CTD is shared by the full-length IE1 and fully depends on the presence of CTD (Figure 4B and C). Further AUC analyses with IE1-CTD mutants showed that they essentially retained the ability to decondense the 30-nm chromatin fiber, possibly due to their incomplete loss of NCP-binding abilities (Figure 4D, E and F). By contrast, chromatin fibers reconstituted with the E56R mutant of histone H2A, previously shown to be unable to interact with IE1-CTD, displayed no alteration of chromatin folding by IE1-CTD (Figure 4G). These observations indicate that the binding of IE1-CTD at the acidic patch of the nucleosome modulates the higher-order structure of chromatin. It should be pointed out that not all acidic patch-binding proteins affect chromatin folding, as LANA does not alter the folding of 30-nm chromatin fiber in our AUC analysis (Figure 4H).

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Our structural analysis revealed that IE1-CTD binds the acidic batch of NCP in a distinct manner. A careful analysis of the landscape of the acidic patch reveals that it can be divided into distinct binding zones that host specific amino acid residues. The conserved binding mode of an arginine in zone I of NCP is important for the association of all NCP-binding partners known to date, while other zones of the acidic patch are involved in differential binding of individual partners. For example, zone III of the acidic patch hosts the binding of His481 of IE1-CTD and Thr14 of LANA, respectively. These two residues interact with Glu56 of histone H2A differently in the structure: while the τ nitrogen of the imidazole ring of His481 makes a hydrogen bond with the carboxylate group of Glu56 of histone H2A at a distance of approximately 2.9 Å, Thr14 of LANA contacts Glu56 of H2A approximately 5 Å away via van der Waals interaction. This difference in peptide-NCP interactions perhaps account for our observations that an E56R mutant affected the binding of IE1-CTD more severely than that with LANA.

For the purpose of dissecting the functions of individual NCP binding partners, one would ideally like to be able to isolate histone mutants that affect the binding of one protein but not others. The most promising such sites appear to lie in zone II and III, as they are less frequently occupied among known NCP binding proteins (Figure 2). However, the task is more challenging to distinguish IE1 and LANA bindings, as they both interact with all binding zones of the acidic patches. Nevertheless, the identification of the E56R variant of histone H2A as an IE1-noninteracting mutant has already served as a useful tool for assessing IE1’s ability to modulate the higher-order structure of chromatin. Interestingly, this chromatin-modulating activity is not shared by LANA. It was shown previously that the LANA peptide promotes the compaction of nucleosomal arrays, judged by an assay in which the folding of nucleosomal arrays was induced by magnesium ions (Chodaparambil et al., 2007). In qualitative agreement with the previous observation, we saw that the addition of LANA made the nucleosomal arrays sedimented slower. Nevertheless, unlike IE1-CTD, LANA has no effect on the preformed H1-containing 30-nm chromatin fiber. There are two possible reasons for the different behavior of LANA, one possibility is that LANA is bound to the chromatin fiber but did not cause any changes to the folding, and the other possibility is that LANA failed to bind 30-nm chromatin fiber. In either case, IE1 and LANA differ in their ability to affect the folding of 30-nm chromatin fiber. The contrasting properties of IE1 and LANA suggest that distinct modes of protein binding to the acidic patch of the nucleosome could exert differential influences to chromatin folding. The discovery of the chromatin modulating activity of IE1 should facilitate further mechanistic understanding of its functions in viral pathogenesis, particularly its impact on the chromatin structure of the host genome, such as transcriptional regulation.

1. 1. Adams PD
   2. Afonine PV
   3. Bunkóczi G
   4. Chen VB
   5. Davis IW
   6. Echols N
   7. Headd JJ
   8. Hung L-W
   9. Kapral GJ
   10. Grosse-Kunstleve RW
   11. McCoy AJ
   12. Moriarty NW
   13. Oeffner R
   14. Read RJ
   15. Richardson DC
   16. Richardson JS
   17. Terwilliger TC
   18. Zwart PH

   (2010) PHENIX : a comprehensive python-based system for macromolecular structure solution

   Acta Crystallographica Section D Biological Crystallography 66:213–221.

   https://doi.org/10.1107/S0907444909052925

   • Google Scholar
2. 1. Ahn J-H
   2. Brignole EJ
   3. Hayward GS

   (1998) Disruption of PML subnuclear domains by the acidic IE1 protein of human cytomegalovirus is mediated through interaction with PML and may modulate a RING finger-dependent cryptic transactivator function of PML

   Molecular and Cellular Biology 18:4899–4913.

   https://doi.org/10.1128/MCB.18.8.4899

   • Google Scholar
3. 1. Armache K-J
   2. Garlick JD
   3. Canzio D
   4. Narlikar GJ
   5. Kingston RE

   (2011) Structural basis of silencing: Sir3 BAH domain in complex with a nucleosome at 3.0 a resolution

   Science 334:977–982.

   https://doi.org/10.1126/science.1210915

   • Google Scholar
4. 1. Arnaudo N
   2. Fernández IS
   3. McLaughlin SH
   4. Peak-Chew SY
   5. Rhodes D
   6. Martino F

   (2013) The N-terminal acetylation of Sir3 stabilizes its binding to the nucleosome core particle

   Nature Structural & Molecular Biology 20:1119–1121.

   https://doi.org/10.1038/nsmb.2641

   • Google Scholar
5. 1. Barbera AJ
   2. Chodaparambil JV
   3. Kelley-Clarke B
   4. Joukov V
   5. Walter JC
   6. Luger K
   7. Kaye KM

   (2006) The nucleosomal surface as a docking station for kaposi's sarcoma herpesvirus LANA

   Science 311:856–861.

   https://doi.org/10.1126/science.1120541

   • Google Scholar
6. 1. Chen P
   2. Zhao J
   3. Wang Y
   4. Wang M
   5. Long H
   6. Liang D
   7. Huang L
   8. Wen Z
   9. Li W
   10. Li X
   11. Feng H
   12. Zhao H
   13. Zhu P
   14. Li M
   15. Wang Q.-f.
   16. Li G

   (2013) H3.3 actively marks enhancers and primes gene transcription via opening higher-ordered chromatin

   Genes & Development 27:2109–2124.

   https://doi.org/10.1101/gad.222174.113

   • Google Scholar
7. 1. Chodaparambil JV
   2. Barbera AJ
   3. Lu X
   4. Kaye KM
   5. Hansen JC
   6. Luger K

   (2007) A charged and contoured surface on the nucleosome regulates chromatin compaction

   Nature Structural & Molecular Biology 14:1105–1107.

   https://doi.org/10.1038/nsmb1334

   • Google Scholar
8. 1. Dimitropoulou P
   2. Caswell R
   3. McSharry BP
   4. Greaves RF
   5. Spandidos DA
   6. Wilkinson GWG
   7. Sourvinos G

   (2010) Differential relocation and stability of PML-body components during productive human cytomegalovirus infection: detailed characterization by live-cell imaging

   European Journal of Cell Biology 89:757–768.

   https://doi.org/10.1016/j.ejcb.2010.05.006

   • Google Scholar
9. 1. Dorigo B
   2. Schalch T
   3. Kulangara A
   4. Duda S
   5. Schroeder RR
   6. Richmond TJ

   (2004) Nucleosome arrays reveal the two-start organization of the chromatin fiber

   Science 306:1571–1573.

   https://doi.org/10.1126/science.1103124

   • Google Scholar
10. 1. Dyer PN
    2. Edayathumangalam RS
    3. White CL
    4. Bao Y
    5. Chakravarthy S
    6. Muthurajan UM
    7. Luger K

    (2003) Reconstitution of nucleosome core particles from recombinant histones and DNA

    Methods Enzymol 375:23–44.

    https://doi.org/10.1016/S0076-6879(03)75002-2

    • Google Scholar
11. 1. Emsley P
    2. Cowtan K

    (2004) Coot : model-building tools for molecular graphics

    Acta Crystallographica Section D Biological Crystallography 60:2126–2132.

    https://doi.org/10.1107/S0907444904019158

    • Google Scholar
12. 1. Gawn JM
    2. Greaves RF

    (2002) Absence of IE1 p72 protein function during low-multiplicity infection by human cytomegalovirus results in a broad block to viral delayed-early gene expression

    Journal of Virology 76:4441–4455.

    https://doi.org/10.1128/JVI.76.9.4441-4455.2002

    • Google Scholar
13. 1. Huh YH
    2. Kim YE
    3. Kim ET
    4. Park JJ
    5. Song MJ
    6. Zhu H
    7. Hayward GS
    8. Ahn J-H

    (2008) Binding STAT2 by the acidic domain of human cytomegalovirus IE1 promotes viral growth and is negatively regulated by SUMO

    Journal of Virology 82:10444–10454.

    https://doi.org/10.1128/JVI.00833-08

    • Google Scholar
14. 1. Kalashnikova AA
    2. Porter-Goff ME
    3. Muthurajan UM
    4. Luger K
    5. Hansen JC

    (2013) The role of the nucleosome acidic patch in modulating higher order chromatin structure

    Journal of the Royal Society Interface 10:20121022.

    https://doi.org/10.1098/rsif.2012.1022

    • Google Scholar
15. 1. Kato H
    2. Jiang J
    3. Zhou B-R
    4. Rozendaal M
    5. Feng H
    6. Ghirlando R
    7. Xiao TS
    8. Straight AF
    9. Bai Y

    (2013) A conserved mechanism for centromeric nucleosome recognition by centromere protein CENP-c

    Science 340:1110–1113.

    https://doi.org/10.1126/science.1235532

    • Google Scholar
16. 1. Kingston IJ
    2. Yung JSY
    3. Singleton MR

    (2011) Biophysical characterization of the centromere-specific nucleosome from budding yeast

    Journal of Biological Chemistry 286:4021–4026.

    https://doi.org/10.1074/jbc.M110.189340

    • Google Scholar
17. 1. Krauss S
    2. Kaps J
    3. Czech N
    4. Paulus C
    5. Nevels M

    (2009) Physical requirements and functional consequences of complex formation between the cytomegalovirus IE1 protein and human STAT2

    Journal of Virology 83:12854–12870.

    https://doi.org/10.1128/JVI.01164-09

    • Google Scholar
18. 1. Lafemina RL
    2. Pizzorno MC
    3. Mosca JD
    4. Hayward GS

    (1989) Expression of the acidic nuclear immediate-early protein (iE1) of human cytomegalovirus in stable cell lines and its preferential association with metaphase chromosomes

    Virology 172:584–600.

    https://doi.org/10.1016/0042-6822(89)90201-8

    • Google Scholar
19. 1. Li G
    2. Margueron R
    3. Hu G
    4. Stokes D
    5. Wang Y-H
    6. Reinberg D

    (2010) Highly compacted chromatin formed in vitro reflects the dynamics of transcription activation in vivo

    Molecular Cell 38:41–53.

    https://doi.org/10.1016/j.molcel.2010.01.042

    • Google Scholar
20. 1. Luger K
    2. Mäder AW
    3. Richmond RK
    4. Sargent DF
    5. Richmond TJ

    (1997a) Crystal structure of the nucleosome core particle at 2.8 a resolution

    Nature 389:251–260.

    https://doi.org/10.1038/38444

    • Google Scholar
21. 1. Luger K
    2. Rechsteiner TJ
    3. Flaus AJ
    4. Waye MMY
    5. Richmond TJ

    (1997b) Characterization of nucleosome core particles containing histone proteins made in bacteria

    Journal of Molecular Biology 272:301–311.

    https://doi.org/10.1006/jmbi.1997.1235

    • Google Scholar
22. 1. Makde RD
    2. England JR
    3. Yennawar HP
    4. Tan S

    (2010) Structure of RCC1 chromatin factor bound to the nucleosome core particle

    Nature 467:562–566.

    https://doi.org/10.1038/nature09321

    • Google Scholar
23. 1. McCoy AJ
    2. Grosse-Kunstleve RW
    3. Adams PD
    4. Winn MD
    5. Storoni LC
    6. Read RJ

    (2007) Phaser crystallographic software

    Journal of Applied Crystallography 40:658–674.

    https://doi.org/10.1107/S0021889807021206

    • Google Scholar
24. 1. McGinty RK
    2. Henrici RC
    3. Tan S

    (2014) Crystal structure of the PRC1 ubiquitylation module bound to the nucleosome

    Nature 514:591–596.

    https://doi.org/10.1038/nature13890

    • Google Scholar
25. 1. McGinty RK
    2. Tan S

    (2015) Nucleosome structure and function

    Chemical Reviews 115:2255–2273.

    https://doi.org/10.1021/cr500373h

    • Google Scholar
26. 1. Mocarski ES
    2. Kemble GW
    3. Lyle JM
    4. Greaves RF

    (1996) A deletion mutant in the human cytomegalovirus gene encoding IE1(491aa) is replication defective due to a failure in autoregulation

    Proceedings of the National Academy of Sciences of the United States of America 93:11321–11326.

    https://doi.org/10.1073/pnas.93.21.11321

    • Google Scholar
27. 1. Mucke K
    2. Paulus C
    3. Bernhardt K
    4. Gerrer K
    5. Schon K
    6. Fink A
    7. Sauer E-M
    8. Asbach-Nitzsche A
    9. Harwardt T
    10. Kieninger B
    11. Kremer W
    12. Kalbitzer HR
    13. Nevels M

    (2014) Human cytomegalovirus major immediate early 1 protein targets host chromosomes by docking to the acidic pocket on the nucleosome surface

    Journal of Virology 88:1228–1248.

    https://doi.org/10.1128/JVI.02606-13

    • Google Scholar
28. 1. Murshudov GN
    2. Vagin AA
    3. Dodson EJ

    (1997) Refinement of macromolecular structures by the maximum-likelihood method

    Acta Crystallographica Section D Biological Crystallography 53:240–255.

    https://doi.org/10.1107/S0907444996012255

    • Google Scholar
29. 1. Nevels M
    2. Brune W
    3. Shenk T

    (2004) SUMOylation of the human cytomegalovirus 72-kilodalton IE1 protein facilitates expression of the 86-kilodalton IE2 protein and promotes viral replication

    Journal of Virology 78:7803–7812.

    https://doi.org/10.1128/JVI.78.14.7803-7812.2004

    • Google Scholar
30. 1. Otwinowski Z
    2. Minor W

    (1997) Processing of x-ray diffraction data collected in oscillation mode

    Macromolecular Crystallography, Pt A 276:307–326.

    https://doi.org/10.1016/S0076-6879(97)76066-X

    • Google Scholar
31. 1. Reinhardt J
    2. Smith GB
    3. Himmelheber CT
    4. Azizkhan-Clifford J
    5. Mocarski ES

    (2005) The carboxyl-terminal region of human cytomegalovirus IE1491aa contains an acidic domain that plays a regulatory role and a chromatin-tethering domain that is dispensable during viral replication

    Journal of Virology 79:225–233.

    https://doi.org/10.1128/JVI.79.1.225-233.2005

    • Google Scholar
32. 1. Schalch T
    2. Duda S
    3. Sargent DF
    4. Richmond TJ

    (2005) X-ray structure of a tetranucleosome and its implications for the chromatin fibre

    Nature 436:138–141.

    https://doi.org/10.1038/nature03686

    • Google Scholar
33. 1. Shin HJ
    2. Kim Y-E
    3. Kim ET
    4. Ahn J-H

    (2012) The chromatin-tethering domain of human cytomegalovirus immediate-early (iE) 1 mediates associations of IE1, PML and STAT2 with mitotic chromosomes, but is not essential for viral replication

    Journal of General Virology 93:716–721.

    https://doi.org/10.1099/vir.0.037986-0

    • Google Scholar
34. 1. Song F
    2. Chen P
    3. Sun D
    4. Wang M
    5. Dong L
    6. Liang D
    7. Xu R-M
    8. Zhu P
    9. Li G

    (2014) Cryo-EM study of the chromatin fiber reveals a double helix twisted by tetranucleosomal units

    Science 344:376–380.

    https://doi.org/10.1126/science.1251413

    • Google Scholar
35. 1. Wang F
    2. Li G
    3. Altaf M
    4. Lu C
    5. Currie MA
    6. Johnson A
    7. Moazed D

    (2013) Heterochromatin protein Sir3 induces contacts between the amino terminus of histone H4 and nucleosomal DNA

    Proceedings of the National Academy of Sciences of the United States of America 110:8495–8500.

    https://doi.org/10.1073/pnas.1300126110

    • Google Scholar
36. 1. Wilkinson GM
    2. Kelly C
    3. Sinclair JH
    4. Rickards C
    5. Rickards C

    (1998) Disruption of PML-associated nuclear bodies mediated by the human cytomegalovirus major immediate early gene product

    Journal of General Virology 79:1233–1245.

    https://doi.org/10.1099/0022-1317-79-5-1233

    • Google Scholar
37. 1. Yang D
    2. Fang Q
    3. Wang M
    4. Ren R
    5. Wang H
    6. He M
    7. Sun Y
    8. Yang N
    9. Xu R-M

    (2013) Nalpha-acetylated Sir3 stabilizes the conformation of a nucleosome-binding loop in the BAH domainn

    Nature Structural & Molecular Biology 20:1116–1118.

    https://doi.org/10.1038/nsmb.2637

    • Google Scholar

Author details

1. Qianglin Fang

   1. National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
   2. College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China

   Contribution

   QF, Prepared nucleosomes and proteins, Performed crystallization and ITC measurements, Acquisition of data, Analysis and interpretation of data, Drafting or revising the article

   Contributed equally with

   Ping Chen

   Competing interests

   The authors declare that no competing interests exist.

2. Ping Chen

   National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China

   Contribution

   PC, Performed AUC experiments and analyzed data, Acquisition of data, Analysis and interpretation of data, Drafting or revising the article

   Contributed equally with

   Qianglin Fang

   Competing interests

   The authors declare that no competing interests exist.

3. Mingzhu Wang

   National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China

   Contribution

   MW, Collected crystal diffraction data and solved the structure, Edited the manuscript, Acquisition of data, Analysis and interpretation of data

   Competing interests

   The authors declare that no competing interests exist.

4. Junnan Fang

   1. National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
   2. College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China

   Contribution

   JF, Performed AUC experiments and analyzed data, edited the manuscript, Acquisition of data, Analysis and interpretation of data

   Competing interests

   The authors declare that no competing interests exist.

5. Na Yang

   1. National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
   2. College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China

   Contribution

   NY, Edited the manuscript, Conception and design, Analysis and interpretation of data

   Competing interests

   The authors declare that no competing interests exist.

6. Guohong Li

   1. National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
   2. College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China

   Contribution

   GL, Conception and design, Analysis and interpretation of data, Drafting or revising the article

   For correspondence

   liguohong@sun5.ibp.ac.cn

   Competing interests

   The authors declare that no competing interests exist.

7. Rui-Ming Xu

   1. National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
   2. College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China

   Contribution

   R-MX, Conception and design, Analysis and interpretation of data, Drafting or revising the article

   For correspondence

   rmxu@sun5.ibp.ac.cn

   Competing interests

   The authors declare that no competing interests exist.

   "This ORCID iD identifies the author of this article:" 0000-0002-6537-4623

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