Figure 4—figure supplement 1. | Spatial patterning of P granules by RNA-induced phase separation of the intrinsically-disordered protein MEG-3

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Spatial patterning of P granules by RNA-induced phase separation of the intrinsically-disordered protein MEG-3

Figure 4—figure supplement 1.

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Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, United States
Figure 4—figure supplement 1.
Download figureOpen in new tabFigure 4—figure supplement 1. Phase Separation assay titration.

DIC images of MEG-3 solutions in the presence of increasing concentrations of protein and RNA. MEG-3 was diluted into phase separation buffer (25 mM HEPES, pH7.4, NaCl adjusted to a final concentration of 150 mM) at room temperature. The solution was transferred to a glass bottom dish and the dish surface was photographed using an inverted DIC microscope 10 min after the initial dilution (room temperature).

DOI: http://dx.doi.org/10.7554/eLife.21337.010