The PIE-scope resolves the limitations in correlative cryo-focused ion beam milling by adding robustness and ease of use to the workflow.

The augmentation of resin-embedded sample blocks with magnetic resin allows remote actuation of ultrathin sections and deposition at high packing density onto silicon wafers for correlative light and electron microscopy.

Immunohistochemical labeling of antigens deep within mouse brain tissue sections was achieved without the need for lipid permeabilization, thereby preserving tissue ultrastructure and enabling correlative light and electron microscopy studies.

SerialFIB is an adaptable open-source software designed for streamlining, advanced development and automation of cryo-focused ion beam-based cellular preparations for in situ cryo-electron tomography.

Multiple imaging modalities resolved the ultrastructure of single hematopoietic stem cells in their endogenous niche, allowing identification of dopamine beta-hydroxylase positive cells as a functional niche cell type.

Quantitative super-resolution correlative light and electron microscopy reveals a constant glycine receptor density at native spinal cord synapses that is maintained in the oscillator mouse model of human hyperekplexia.

Building on previous work (Picco et al., 2015), we use a hybrid imaging approach to address the role of clathrin during endocytosis: we find that it has no role in shaping or elongating membrane invaginations, but it does contribute to the regularity of vesicle scission.

The development of high-resolution correlative imaging of metals and proteins reveals the interaction of copper and zinc with the synaptic architecture of neurons.

Correlative microscopy and electron cryo-tomography on apoptotic HeLa cells reveal remodeling of outer and inner mitochondrial membranes, Bax cluster ultrastructure and ATP synthase reorganization in ruptured mitochondria.

Integrating a cryogenic light microscope with a focused ion beam/scanning electron microscope, allows directly targeting of fluorescent structure when preparing a frozen-hydrated lamella, without the need for repositioning or fiducial markers.