Minimal genetic modifications of endogenous mosquito genes allow the tissue-specific expression of anti-malarial effectors and convert them into efficient non-autonomous gene drives.

If released in the wild, current CRISPR-based gene drive systems designed to alter populations could spread much farther than intended, despite the evolution of drive resistance.

Genes drives utilizing synthetic target sites and the split-Cas9 system have similar performance to standard drives.

Generation of a home-and-rescue gene drive targeting an essential gene reveals confinable population modification.

Selfish genetic elements may either promote or prevent the evolution of reproductive barriers, depending on the extent of gene flow between species.

A Y-chromosome shredding gene drive that depletes the pool of XY males and effects mate limitation could be a viable tool for vertebrate pest control.

Combining CRISPR/Cas9 genome editing with gene drives may enable scientists to reversibly edit the genomes of diverse wild populations, an advance that could help eliminate diseases, support sustainable agriculture, and control invasive species.

Bidirectional promoter activity, which is inherent to the promoters of selection cassettes used for genome editing, causes divergent transcription that can disrupt expression of genes neighboring cassette insertion sites, causing potent off-target effects.

Highly efficient and confinable split gene drives are a new tool for the control of the dengue mosquito vector.

Strong reduction in malaria transmission by genetically modified mosquitoes is achieved in the laboratory using a dual gene drive that simultaneously propagates an antiparasitic factor and a parasite-detrimental mosquito mutation.