2,772 results found
    1. Cell Biology
    2. Neuroscience

    A library of MiMICs allows tagging of genes and reversible, spatial and temporal knockdown of proteins in Drosophila

    Sonal Nagarkar-Jaiswal, Pei-Tseng Lee ... Hugo J Bellen
    A large collection of functional EGFP tagged proteins derived from MiMIC insertions allows examination of protein expression in unfixed tissues and efficient tissue specific reversible knock down of proteins.
    1. Cell Biology
    2. Neuroscience

    A genetic toolkit for tagging intronic MiMIC containing genes

    Sonal Nagarkar-Jaiswal, Steven Z DeLuca ... Hugo J Bellen
    Building on previous work (Nagarkar-Jaiswal et al., 2015), we report an elegant genetic strategy for endogenous tagging of MiMIC containing genes.
    1. Developmental Biology
    2. Neuroscience

    A cell cycle-independent, conditional gene inactivation strategy for differentially tagging wild-type and mutant cells

    Sonal Nagarkar-Jaiswal, Sathiya N Manivannan ... Hugo J Bellen
    A conditional gene inactivation system that permits the tracking of protein expression, and creates fluorescently-labelled mutant cells in both developing and adult Drosophila tissues.
    1. Cell Biology

    Age-dependent deterioration of nuclear pore assembly in mitotic cells decreases transport dynamics

    Irina L Rempel, Matthew M Crane ... Liesbeth M Veenhoff
    In replicative ageing yeast cells, an age-dependent impediment in proper assembly of nuclear pore complexes is associated with altered nuclear transport.
    1. Genetics and Genomics

    An expanded toolkit for gene tagging based on MiMIC and scarless CRISPR tagging in Drosophila

    David Li-Kroeger, Oguz Kanca ... Hugo J Bellen
    New technology for manipulating genes in Drosophila facilitates gene tagging and precise modification of virtually any gene.
    1. Cell Biology
    2. Microbiology and Infectious Disease

    Malaria parasite LIMP protein regulates sporozoite gliding motility and infectivity in mosquito and mammalian hosts

    Jorge M Santos, Saskia Egarter ... Gunnar R Mair
    A small surface protein regulates gliding motility and host cell invasion by the sporozoite stage of the malaria parasite.
    1. Genetics and Genomics

    Loss of centromere function drives karyotype evolution in closely related Malassezia species

    Sundar Ram Sankaranarayanan, Giuseppe Ianiri ... Kaustuv Sanyal
    The centromeres in Malassezia species, by breakage or inactivation, facilitate genome rearrangements that can result in varying karyotypes and contribute to the evolution of these species.
    1. Computational and Systems Biology
    2. Chromosomes and Gene Expression

    Avoidance of stochastic RNA interactions can be harnessed to control protein expression levels in bacteria and archaea

    Sinan Uğur Umu, Anthony M Poole ... Paul P Gardner
    Stochastic mRNA and non-coding RNA hybridisation has a major impact on protein expression.
    1. Cell Biology
    2. Neuroscience

    Dynamics and nanoscale organization of the postsynaptic endocytic zone at excitatory synapses

    Lisa AE Catsburg, Manon Westra ... Harold D MacGillavry
    The postsynaptic endocytic zone is a highly organized structure composed of a diversity of endocytic proteins.
    1. Cell Biology

    Selective sorting and destruction of mitochondrial membrane proteins in aged yeast

    Adam L Hughes, Casey E Hughes ... Daniel E Gottschling
    A new mitochondrial protein degradation pathway selectively sorts and destroys a subset of the mitochondrial membrane proteome to protect mitochondria in times of stress.

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