Chandra M Khantwal, Sherwin J Abraham ... Merritt Maduke
The characterization of a previously unidentified “outward-facing open” conformational state provides a new framework for understanding the CLC transport mechanism.
Crystallography together with electron-resonance spectroscopy, molecular-dynamics simulations, and transport measurements reveal the molecular details of protein conformational change, and how this change contributes to function in a CLC-type chloride/proton exchanger.
Combined simulations and electrophysiological experiments show that the CLC channels and exchangers form physically distinct and evolutionarily conserved pathways through which Cl- and H+ ions move when crossing biological membranes.
Nicolai J Toft, Trine V Axelsen ... Ebbe Boedtkjer
Cellular acidity, capacity for net acid extrusion, and expression of acid-base transporters in human breast carcinomas independently predict variation in proliferative activity, lymph node metastasis, and patient survival.
The H+ influx coupled to nutrient uptake and the plasma membrane H+-ATPase are central actors of the activation of target of rapamycin complex 1 (TORC1) in budding yeast Saccharomyces cerevisiae..
The cryo-EM structure and functional characterization of the chloride-selective ion transporter Slc26a9 defines its oligomeric architecture and transport mechanism.
Helen Hertenstein, Ellen McMullen ... Stefanie Schirmeier
Detailed analysis of transporter expression and glucose uptake rates shows that carbohydrate import at the blood–brain barrier is adapted via TGF-βsignaling-dependent transcriptional regulation of carbohydrate transporters to protect the brain form effects of nutrient shortage.