Micropatterned differentiation of mouse pluripotent stem cells gives rise to regionally distinct cell types arising in embryos at gastrulation.

Activation of Wnt/β-catenin signaling in endothelial progenitors derived from human pluripotent stem cells partially induces the specialized blood–brain barrier phenotype while the same treatment in matured endothelial cells is less efficacious.

Identifying the pathways that support human naive-state pluripotent stem cells provides insights into the signalling-based regulation of human pluripotency and enables informed decisions to improve conditions for pluripotent cell culture.

A panel of chimpanzee induced pluripotent stem cells (iPSCs) will help realise the potential of iPSCs in primate studies, and in combination with genomic technologies, transform studies of comparative evolution.

Naive hPSCs can readily give rise to human trophoblast stem cells, thus demonstrating their extraembryonic lineage potential and providing a new model system to study human trophectoderm specification.

CEP83 plays a central role in regulating the development of intermediate mesoderm (IM) nephron progenitors, which may involve direct effects of CEP83 in the nephron progenitor differentiation program and indirect lateral plate mesoderm-mediated effects on the IM.

Selective activation of FZD7 signaling with an engineered WNT mimetic promotes early developmental programs, including endodermal lineage specification, in human pluripotent stem cells.

Treatment of human pluripotent stem cell-derived myotubes with a cocktail of small molecules induces their maturation, as shown by gene expression, biochemical and functional assays.

Directed differentiation of stem cells can generate ventral-anterior foregut spheroids that can expand into three-dimensional lung organoids with striking structural, cellular and molecular similarities to the human fetal lung.

In vitro model of germline specification in marmosets established by directed differentiation of induced pluripotent stem cells, which transcriptionally resemble pre-migratory primordial germ cells in vivo.