New functionality in RELION-4.0 allows convenient structure determination from cryo electron tomography data to resolutions sufficient for de novo atomic modelling.

Phase contrast X-ray tomography based on a combination of parallel and cone beam geometry extends conventional histology by a third dimension and enables full quantication of tissue remodeling in COVID-19.

The automation of cryo-focused ion beam milling enables previously unfeasible cryo-electron tomography projects.

X-ray phase-contrast tomography reveals pathology of capillaries in heart tissue from patients who succumbed to Covid-19, as well as alterations in the three-dimensional cardiac tissue structure.

A detailed description of the structure of procentriole MT triplet by cryoET, along with its associated non-tubulin proteins and its assembly intermediates, reveals possible molecular mechanism for the procentriole assembly.

Cryo electron tomography provides the first high-resolution 3D axoneme structure from any pathogenic organism, revealing novel structures that support the unique motility of these pathogens through host tissues.

Direct observation of RNA Polymerase II transcription through a single nucleosome at near basepair resolution suggests a mechanism for selective control of gene expression.

Topographic maps can gradually increase the fidelity of sensory representations and improve signal-to-noise ratio across multiple cortical circuits, a generic architectural feature that depends solely on the modularity of topographic projections and recurrent inhibition.

SI topographic finger maps of older adults show signs of cortical aging but do not show classical hallmarks of cortical de-differentiation.

Ultrastructural analysis of optogenetically stimulated inner hair cell ribbon synapses reveals normally sized synaptic vesicles and enhanced vesicle docking at the active zone membrane.