AlphaFold-Multimer was used to investigate extracellular protein interactions involved in mammalian egg-sperm recognition, suggesting a putative pentameric complex that includes TMEM81, a sperm protein not previously involved in gamete recognition.

Conditional deletion of Bmal1 in the forebrain/SCN reveals an essential role of a functional master clock in maintaining synchronized and high amplitude rhythms of peripheral clocks.

TMEM95, a protein localized to the sperm acrosome cap, is essential for fertilization.

A plant autophagy pathway transports chloroplast stroma and envelope components into the vacuole through the division and encapsulation of the site of chloroplasts that is associated with developing autophagosome.

TIRF microscopy in living pancreatic beta cells lacking one or two of Rab27 effectors and the exocyst components reveals how each molecule functions in sequence or in parallel in multiple redundant paths and rate-limiting processes in granule exocytosis.

Analyses of knockout mice and a biochemical screen of interacting proteins reveal that exophilin-8 forms a large network of protein interactions to efficiently retain secretory granules beneath the plasma membrane for exocytosis.

Highly specific gene manipulation in mouse thymic epithelial cells (TECs) shows that fine-tuning of β-catenin expression is required for TECs to generate an optimal thymic microenvironment to support T-cell development.

Thymocyte-attracting CCL21-expressing embryonic mTECs carry a developmental potential to give rise to self-antigen-displaying Aire-expressing mTECs.

Responses to odour mixtures are more discriminable in awake, behaving mice due to a combination of dampened responses keeping responses in the linear range and pattern decorrelation, with these two mechanisms operating on different timescales.

Pathogenic LRRK2kinase requires Rab10 and RILPL1 to block primary cilia formation, shortening cilia on cholinergic neurons needed for a hedgehog driven circuit that supports dopaminergic neurons in mouse brain.