Genome-wide profiling of R-loops at near single-nucleotide resolution reveals distinct R-loop boundaries depending on the presence and location of the first exon-intron junction.

Genetic analyses in budding yeast coupled with biochemical assays have revealed an unexpected role for the Smc5/6 complex in cellular pathways responsible for the prevention of RNA formation in genomic DNA.

BisMapR reveals strand-specific R-loops with high resolution at small-scale genomic features including bidirectionally transcribed promoters and enhancers.

The transcriptional response elicited by the hormone estrogen leads to DNA double-strand breaks by promoting R-loop formation in breast cancer cells.

The deposition of 5-hydroxymethylcytosine epigenetic marks in active genes promotes the annealing of the nascent RNA to the template DNA strand, forming an R-loop.

The newly developed antibody-free techniques, HepG4-seq for G-quadruplexes and HBD-seq for R-loops, robustly reveal the comprehensive maps of native G-quadruplexes and R-loops, as well as their roles in transcriptional regulation.

Investigation of the role of triple-stranded nucleic acid, R-loops, in HIV-1 genome integration.

STAG proteins function independently of core cohesin at R-loops to facilitate cohesin loading.

RNA polymerase recruits the PcrA DNA helicase via a conserved interaction motif in order to remove R-loops and prevent conflicts with replication.

Reconstitution of orientation-specific R-loop-replisome collisions with purified proteins reveals the differential impact of R-loop-associated RNA:DNA hybrids and G-quadruplexes on replication fork progression.